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    Wyświetlanie 1-4 z 4
    Tytuł:
    Different MACS sorting strategies for the enrichment of Lin– (CD34+ CD45–) hematopoietic progenitor cells: preliminary study
    Autorzy:
    Vašíček, Jaromír
    Baláži, Andrej
    Parkányi, Vladimír
    Bauer, Miroslav
    Powiązania:
    https://bibliotekanauki.pl/articles/2183569.pdf
    Data publikacji:
    2018-12-31
    Wydawca:
    Uniwersytet Pedagogiczny im. Komisji Edukacji Narodowej w Krakowie
    Tematy:
    CD34
    CD45
    flow cytometry
    hematopoietic stem cells
    MACS
    qPCR
    rabbit
    Opis:
    Magnetic-activated cell sorting (MACS) has become a standard method for the isolation of hematopoietic stem/progenitor cells (HSC/HPC) in human or mouse model using CD34 antibodies. However, at the present there is no useable CD34 antibody that could be successfully used for the selection of rabbit HSC/HPC. Therefore, the aim of this preliminary study was to remove all mature cells (CD45+) from the heterogeneous mixture of rabbit peripheral blood and bone marrow mononuclear cells (PBMCs and BMMCs) in order to enrich these cell populations for the CD34+ cells. Briefly, cells were incubated with a CD45 antibody and proper magnetic microbeads. Three different MACS sorting strategies were used in the experiment that differed mainly in the sample loading rate and the number of used magnetic columns. Control (unsorted) and sorted cells were assessed for the sorting efficiency (% of double positive cells for CD45 and Labelling Check Reagent - LCR) by flow cytometry and for the relative expression of CD34 antigen by qPCR. According to flow cytometry, Depl025 mode showed the best sorting efficiency in terms of the lowest percentages of CD45+LCR+ cells for rabbit PBMCs as well as BMMCs. qPCR analysis confirmed this mode as the best in terms of the relative CD34 expression for rabbit PBMCs. However, higher relative expression of CD34 in BMMCs was obtained by other mode - Posselds. In conclusion, this study demonstrates a possible enrichment of rabbit (CD34+) HSC/HPC by the magnetic depletion of mature hematopoietic (CD45+) cells.
    Źródło:
    Annales Universitatis Paedagogicae Cracoviensis Studia Naturae; 2018, 3 (suppl.); 83-89
    2543-8832
    2545-0999
    Pojawia się w:
    Annales Universitatis Paedagogicae Cracoviensis Studia Naturae
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Effects of Dissipation and Noise on Chaotic Transport in Superlattices
    Autorzy:
    Balanov, A.
    Greenaway, M.
    Fromhold, T.
    Powiązania:
    https://bibliotekanauki.pl/articles/1585111.pdf
    Data publikacji:
    2009-11
    Wydawca:
    Polska Akademia Nauk. Instytut Fizyki PAN
    Tematy:
    73.21.Cd
    72.20.Ht
    05.45.-a
    Opis:
    We study the effects of dissipation and noise on chaotic electron dynamics, which accompany charge transport in semiconductor superlattices with an applied bias voltage and a tilted magnetic field. We consider the evolution of different chaotic trajectories as decoherence increases, and show that below a critical level of the dissipation rate, dissipative chaos plays an important role in the electron transport. However, by increasing the dissipation rate above the critical level, chaotic dynamics disappear and electrons only demonstrate regular motion. We also investigate how the presence of random fluctuations affects magnetotransport in superlattices and reveal a counter-intuitive non-monotonic dependence of electron drift velocity upon the noise intensity.
    Źródło:
    Acta Physica Polonica A; 2009, 116, 5; 733-740
    0587-4246
    1898-794X
    Pojawia się w:
    Acta Physica Polonica A
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Metallurgical, Mechanical and Electrochemical Behavior Study of the Lamellar Gray Cast Iron Treated with Vanadium
    Autorzy:
    Azzoug, M.
    Boutarek, N.
    Drıf, R.
    Madaouı, N.
    Boulgheb, K.
    Powiązania:
    https://bibliotekanauki.pl/articles/1398720.pdf
    Data publikacji:
    2016-04
    Wydawca:
    Polska Akademia Nauk. Instytut Fizyki PAN
    Tematy:
    68.35.bd
    81.05.uf
    37.10.-x
    81.40.Cd
    64.75.Nx
    82.45.Bb
    Opis:
    This study is concerned with the research and development in the field of materials science, leading to the industrial applications especially for the development of materials by moulding process. The purpose of this study was to determine the influence of vanadium on microstructural, mechanical and electrochemical properties of gray cast iron with lamellar graphite EN-FGL250. We investigated the effect of adding element (vanadium), during the casting of the metal in the mould, in a powder form having a particle size of 0.5 mm with the amounts of 1%, 3% and 5% in weight percent on microstructural and mechanical properties of gray cast iron with lamellar graphite. The originality of this work is the addition of the vanadium powder during the last stage of cooling of the melted gray cast iron EN-FGL250. These additions have a significant impact on the solidification phenomenon since the deposited vanadium powder into the sand moulds creates the new sites of nucleation and absorbs a lot of heat leading to the fast cooling. From the experimental results, we can confirm that the cooling rate directly affects the microstructural, mechanical and electrochemical behavior of the cast gray iron treated with vanadium. As result, it was observed that there is a slight decrease of the elasticity modulus of the work pieces, and a reduction of the maximum tensile resistance R_{m}. Finally, the addition of vanadium considerably reduces the corrosion current of gray cast iron treated with vanadium.
    Źródło:
    Acta Physica Polonica A; 2016, 129, 4; 548-551
    0587-4246
    1898-794X
    Pojawia się w:
    Acta Physica Polonica A
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Influence of Cu Layer Thickness on Morphology and Magnetic Properties of Co/Cu Nanowires
    Autorzy:
    Kac, M.
    Kopec, M.
    Cieniek, L.
    Zarzycki, A.
    Kac, S.
    Maximenko, A.
    Dutkiewicz, E.
    Marszalek, M.
    Powiązania:
    https://bibliotekanauki.pl/articles/1030657.pdf
    Data publikacji:
    2018-02
    Wydawca:
    Polska Akademia Nauk. Instytut Fizyki PAN
    Tematy:
    75.30.Gw
    75.60.Ej
    75.75.Cd
    78.67.Rb
    81.07.Gf
    82.45.Qr
    Opis:
    We studied changes of morphology and magnetic properties of Co/Cu multilayered nanowires, electrodeposited in polycarbonate membranes, as a function of Cu layer thickness. The morphology and structure of wire assemblies with an average diameter of 200 nm and length of 10 μ m, investigated by X-ray diffraction and scanning electron microscopy techniques, revealed polycrystalline structure of Cu and Co layers with smooth lateral surface of nanowires. Overdeposited nanowires created caps which showed flower-like dendrites with shape changing as a function of Cu thickness and electrodeposition parameters. Chemical composition of Co and Cu nanowires analysed by energy dispersive spectroscopy and proton induced X-ray emission showed Cu nanowires free from Co atoms while in Co nanowires, Cu contamination with concentration below 10% was observed. The oxidation traces observed in single-component Cu nanowires did not appear in multilayered nanowires. Magnetic measurements indicated easy axis of magnetization in membrane plane for nanowires with Cu thickness smaller than 20 nm, whereas for larger Cu thicknesses isotropic orientation of magnetization was observed. The presence of Cu atoms in single-component Co nanowires resulted in the appearance of magnetic anisotropy with easy axis along nanowire axis and the increase of coercivity value.
    Źródło:
    Acta Physica Polonica A; 2018, 133, 2; 302-305
    0587-4246
    1898-794X
    Pojawia się w:
    Acta Physica Polonica A
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
      Wyświetlanie 1-4 z 4

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