Temat: Brucella melitensis - Katalog OPAC zbiorów

Skocz do pozycji: 1.

Tytuł:: Molecular cloning of the OMP19 gene from Brucella melitensis strain H38 and its antigenicity compared to that of commercial OMP19
Autorzy:: Uslu, A.
Sanioglu Golen, G.
Agah Tekindal, M.
Sakmanoglu, A.
Sayın, Z.
Denizli, O.
Gok, A.
Erganis, O.
Powiązania:: https://bibliotekanauki.pl/articles/16630377.pdf
Data publikacji:: 2022
Wydawca:: Polska Akademia Nauk. Czasopisma i Monografie PAN
Tematy:: rOMP19 antigen
Brucella melitensis
recombinant protein
Opis:: Brucellosis is a worldwide zoonosis, that can still be classified as endemic despite its ancient origins which causes economic losses and public health problems. Although effectively controlled by vaccination in animals, there is currently no vaccine for use in humans. Outer Membrane Proteins (OMP) that play an active immunogenic and protective role in the Brucellae family. OMP19 is present in all Brucella species as a surface antigen and is a potent immunogen responsible for Brucellosis intracellular infection. For this reason, the study was aimed to be used safely as a potential recombinant vaccine candidate against all Brucella infections, especially in humans and pregnant animals. This study evaluated a Brucella lipoprotein antigen, i.e. 19 kilodalton (kDa) outer membrane protein (OMP19), which was amplified and cloned into the pETSUMO vector system. The immunogenic power of the purified recombinant OMP19 antigen against brucellosis was compared with that of OMP19 (Raybiotech Inc, USA) in a mouse model and the obtained rOMP19 antigen was found to be similar to the commercially available recombinant protein.
Źródło:: Polish Journal of Veterinary Sciences; 2022, 25, 4; 561-569
1505-1773
Pojawia się w:: Polish Journal of Veterinary Sciences
Dostawca treści:: Biblioteka Nauki

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Skocz do pozycji: 2.

Tytuł:: Identification of Brucella melitensis from camel’s blood by vitek2 and real time polymerase chain reaction
Autorzy:: Manivannan, Kavitha
Ramasamy, Malathi
Sundaresan, Uma
Moustafa, Samar M.
Sherloumay
Mariyam, Safna
Powiązania:: https://bibliotekanauki.pl/articles/38695730.pdf
Data publikacji:: 2024-03-30
Wydawca:: Uniwersytet Rzeszowski. Wydawnictwo Uniwersytetu Rzeszowskiego
Tematy:: Brucella melitensis
brucellosis
camels
conventional PCR
RT-PCR
serological assays
Opis:: Introduction and aim. Brucellosis is a zoonotic disease. Experimental clinical and laboratory diagnosis is still facing problems in identifying the organism. The present study will diagnose a Brucella infection in camel blood in Qatar using serological assays. Isolation and identification were performed on a camel blood sample. Brucella in bacterial isolates was determined by real-time polymerase chain reaction (RT-PCR) as a gold standard test. Material and methods. A total of 220 samples, 200 random serum samples, and 20 EDTA blood samples were selected among the above-mentioned random samples, and 20 serum samples from camel handlers were collected from Al Shahaniya province, Qatar. The Rose Bengal test (RBT), buffered antigen plate agglutination test (BAPAT), and enzyme linked immunosorbent assay (cELISA) for the monoclonal antibody in serum samples were performed using commercially available kits. For the molecular detection of Brucella, conventional PCR and real-time PCR (GPS kit) were used for the genus-specific insertion sequence IS711. Brucella melitensis (MICROBOSS Hightech GmbH kit) was used to identify subspecies. Results. The results identified by vitek2 compact (30%) showed B. melitensis in 6 samples out of 20 isolates. Both conventional (66.67%) and RT-PCR (83.33%) analyses supported this, demonstrating the presence of Brucella. These tests also showed that Brucella species were present in Rose Bengal 182/200 (91%), BAPAT 182/200 (91%), and cELISA (90%) 180/200 in camel serum. Conclusion. To conclude, the prevalence of brucellosis in dromedary camels is higher in this region, and as a matter of urgency, measures should be taken to control the disease.
Źródło:: European Journal of Clinical and Experimental Medicine; 2024, 22, 1; 94-101
2544-2406
2544-1361
Pojawia się w:: European Journal of Clinical and Experimental Medicine
Dostawca treści:: Biblioteka Nauki

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Skocz do pozycji: 3.

Tytuł:: Brucellosis in humans - etiology, diagnostics, clinical forms
Autorzy:: Galinska, E.M.
Zagorski, J.
Powiązania:: https://bibliotekanauki.pl/articles/50412.pdf
Data publikacji:: 2013
Wydawca:: Instytut Medycyny Wsi
Tematy:: brucellosis
human disease
etiology
diagnostics
clinical form
Brucella
occupational exposure
serological diagnostics
etiological factor
Brucella melitensis
Brucella abortus
Brucella suis
Brucella canis
Brucella neotomae
Brucella ovis
Brucella marina
Brucella microti
Brucella inopinata
Opis:: Brucellosis in humans is a zoonosis of greatly varied clinical image. It occurs on all inhabited continents. The course of the disease may be acute, sub-acute or chronic. The etiologic factors of brucellosis are small, aerobic Gram-negative rods of the genus Brucella, which currently contains ten species: B. abortus, B. suis, B. ovis, B. melitensis, B. canis, B. neotomae, B. pinnipedialis, B. ceti, B. microti and B. inopinata. In humans, the disease is caused mainly by: B. melitensis as the most pathogenic species, followed by B. suis, whereas B. abortus is considered as the mildest type of brucellosis. The natural reservoir of the germ and the source of infection in humans are infected domestic animals, primarily cattle, sheep, goats, as well as wild animals. Infection in humans occurs by penetration through damaged skin, conjunctiva, and more rarely via the alimentary route by the consumption of infected products. Especially exposed are: veterinarians, veterinary technicians, insemination service employees, zoo technicians, farmers working on multi-herd farms (production cooperatives), e.g. cattlemen, also private farmers, employees of slaughter houses and meat processing enterprises. A basis for diagnosing brucellosis are serologic tests which allow the detection of antibodies occurring in response to infection, performed with the use of the following methods: agglutination test, complement fixation test, Coombs test, 2-mercaptoethanol agglutination test, and Burnet’s intradermal allergy test which detects the state of hypersensitivity of the infected organism to Brucella abortus rods.
Źródło:: Annals of Agricultural and Environmental Medicine; 2013, 20, 2
1232-1966
Pojawia się w:: Annals of Agricultural and Environmental Medicine
Dostawca treści:: Biblioteka Nauki

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Skocz do pozycji: 4.

Tytuł:: Integrated microsystem for multiplexed genosensor detection of biowarfare agents
Autorzy:: Ema, Sagbor Grinity
Mwadwo, Kwumasi Sylvester
Paul, Bosu P.
Powiązania:: https://bibliotekanauki.pl/articles/1119284.pdf
Data publikacji:: 2016
Wydawca:: Przedsiębiorstwo Wydawnictw Naukowych Darwin / Scientific Publishing House DARWIN
Tematy:: Bacillus anthracis
Bacteriophage lambda
Brucella melitensis
Burkholderia mallei
Coxiella burnetii
Electrochemical DNA biosensor. Biowarfare agent
Francisella tularensis
Multiplex detection
Self-assembled monolayer (SAM)
Yersinia pestis
Opis:: An early, rapid and definite detection for the presence of biowarfare agents, pathogens, viruses and toxins is required due to their harmful effect to human population. Those potentially encountering the aforementioned include people involved in civil rescue and security, homeland security, military operations, as well as public transportation securities such as airports, metro and railway stations. This work informs the reader of an electrochemical genosensor with an integrated microsystem array combined with microtube fluidics that allows simultaneous detection of different biowarfare agents such as Bacillus anthracis, Brucella melitensis, Bacteriophage lambda, Francisella tularensis, Burkholderia mallei, Coxiella burnetii, Yersinia pestis, and Bacillus thuringiensis var. kurstaki. The chip electrode arrays were modified via coimmobilisation of a 1:100 (mol/mol) mixture of a thiolated probe and a polyethyleneglycol terminated bipodal thiol. Herein, PCR products from relevant biowarfare agents were detected reproducibly through a sandwich assay format with the target hybridised between a surface immobilised probe into the electrode and a horseradish peroxidase-labelled secondary reporter probe, which provided an enzyme based electrochemical signal. Cross-reactivity studies over potential interfering DNA sequences have demonstrated high selectivity using the developed platform producing high-throughput.
Źródło:: World News of Natural Sciences; 2016, 5; 20-32
2543-5426
Pojawia się w:: World News of Natural Sciences
Dostawca treści:: Biblioteka Nauki