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    Wyświetlanie 1-6 z 6
    Tytuł:
    Effects of Rhizobium, Nitrogen and Phosphorus Fertilizers on Growth, Nodulation, Yield and Yield Attributes of Soybean at Pawe Northwestern Ethiopia
    Autorzy:
    Tarekegn, Masresha Abitew
    Kibret, Kibebew
    Powiązania:
    https://bibliotekanauki.pl/articles/1178676.pdf
    Data publikacji:
    2017
    Wydawca:
    Przedsiębiorstwo Wydawnictw Naukowych Darwin / Scientific Publishing House DARWIN
    Tematy:
    Bradyrhizobium japonicum
    Fertilizers
    Nitrogen Fixation
    Opis:
    Owing to the rising costs of chemical fertilizers and the growing environmental concerns, there is an ever increasing interest in the role of soil microorganisms in crop nutrition and soil fertility restoration. A field study was therefore conducted at Pawe Agricultural Research Center during 2010 main cropping season to determine the influence of Bradyrhizobium japonicum inoculation and N and P fertilizers application on nodulation, yield and yield attributes of soybean. Three levels of N (0, 11.5 and 23 kg N ha-1); three levels of P (0, 23 and 46 kg P2O5 ha-1) with two levels of Rhizobium were arranged in RCBD in factorial combinations with three replications. Nodule number, nodule fresh and dry weights, plant height, number of pods and seeds per plant, 100 seeds weight and grain yield responded significantly to the interaction effects of B. japonicum inoculation and application of N and P fertilizers but nodule volume, seed and biomass yields, and harvest index were significantly affected by the main effects of any one or more of the factors and interaction of any two of the factors. The maximum numbers of nodules of 80.26, fresh and dry weights of 3.77 and 0.99 gm./plant respectively; were measured by combined effect of 11.5 kg N/ha, 46 kg P2O5/ha and B. japonicum. Similarly the highest nodule volume of 3.53 and 3.27 ml/plant were measured after applications of 46 kg P2O5/ha with B. japonicum and with 11.5 kg N/ha respectively. The highest plant heights of 79.26 cm followed by 76.94 cm were measured by application of 46 kg P2O5/ha with B. japonicum and 46 kg P2O5/ha itself respectively. Seed yield significantly increased to 11.91 gm /plant and 15.97 gm /plant following application of B. japonicum alone and 46 kg P2O5/ha with B. japonicum respectively. Applied 23 kg/ha brought the highest plant biomass of 27.25 gm/plant. Inoculation with 46 kg P2O5/ha and the combined effects of 11.5 kg N/ha, 46 kg P2O5/ha and B. japonicum resulted 73.93 and 80.66 number of pods per plant respectively. The maximum 100.60 numbers of seeds per plant were counted during inoculation of B. japonicum with 46 kg P2O5/ha. The maximum 100-seed weight of 16.96 gm and grain yield of 3151.88 kg/ha were produced by combined application of 11.5 kg N/ha, 46 kg P2O5/ha and B. japonicum. Each nodule attributes were significantly and positively correlated each other and with each yield and yield attributes. The results showed that growth and yield potential of soybean and an increase N2 fixing can be achieved by using B. japonicum and P fertilizer with reduced level of N as starter fertilizer.
    Źródło:
    World Scientific News; 2017, 67, 2; 201-218
    2392-2192
    Pojawia się w:
    World Scientific News
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Mass spectrometry identification of membrane-bound respiratory nitrate reductase from Bradyrhizobium sp. (Lupinus)
    Autorzy:
    Polcyn, Władysław
    Powiązania:
    https://bibliotekanauki.pl/articles/1040683.pdf
    Data publikacji:
    2008
    Wydawca:
    Polskie Towarzystwo Biochemiczne
    Tematy:
    immunoblot screening
    membrane-bound nitrate reductase [EC 1.7.99.4]
    enzyme purification
    Bradyrhizobium sp. (Lupinus)
    rhizobia
    mass spectrometry sequencing
    Opis:
    Respiratory nitrate reductase (NR) from Bradyrhizobium sp. (Lupinus) USDA 3045 has biochemical properties of the membrane-bound NR type. However, in the completely sequenced rhizobium genomes only genes for the periplasmic type of dissimilatory NR were found. Therefore purification and identification of the enzyme by tandem mass spectrometry (MS/MS) was undertaken. MS/MS spectra representing 149 unique tryptic peptides derived from purified 137-kDa subunit matched the NCBInr-deposited NarG sequences. MS/MS sequencing of two other SDS/PAGE bands (65- and 59-kDa) identified them as derivatives of the NarH-type protein. Applying additional validation criteria, 73% of the sequence of the NarG subunit (902 aa) and 52% of NarH sequence (266 aa) was assembled (UniProt KB acc. no. P85097 and P85098). This is the first unambiguous identification of an active NarGH-like NR in rhizobia. Moreover, arguments are provided here for the existence of a functional enzyme of this type also among other rhizobial species, basing on immunoblot screening and the presence of membrane-associated NR-active electrophoretic forms.
    Źródło:
    Acta Biochimica Polonica; 2008, 55, 4; 753-760
    0001-527X
    Pojawia się w:
    Acta Biochimica Polonica
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Nitrate-related down-regulation of respiratory nitrate reductase from Bradyrhizobium sp. (Lupinus)
    Autorzy:
    Polcyn, Władysław
    Powiązania:
    https://bibliotekanauki.pl/articles/1040684.pdf
    Data publikacji:
    2008
    Wydawca:
    Polskie Towarzystwo Biochemiczne
    Tematy:
    multiple enzyme forms
    membrane-bound nitrate reductase [EC 1.7.99.4]
    down-regulation
    nitrate and nitrite induction
    stability of subunits
    Bradyrhizobium sp. (Lupinus)
    Opis:
    Previously, we showed that anaerobic induction of respiratory nitrate reductase (NR) activity in Bradyrhizobium sp. (Lupinus) USDA 3045 is strongly enhanced by nitrate or nitrite through de novo synthesis. Here, multiple NR-active soluble forms, ranging from 75 kDa to 190 kDa, were observed under anaerobic conditions. Electrophoretic activity band patterns differed depending on the level and the type of the N oxyanion added. The intensity of the membrane-bound NR activity band of 230 kDa changed with time along with consumption of 2 mM nitrate. It was associated with a parallel 5-fold increase and then 2-fold reduction in the amount of membrane-bound NR protein. In contrast, on 4 mM nitrate, the level of NR protein was much more stable, apparently due to slower nitrate depletion. Moreover, in cells anaerobically grown without nitrate addition, a 42-kDa derivative of NR degradation was immunodetected, which was not observed if nitrate was present in the medium. These findings suggest that the amount of the respiratory NR protein could be negatively regulated by endogenous proteases in relation to the level of nitrate available. It seems, therefore, that multiple native forms might be not different isoenzymes but immature complexes or derivatives of the enzyme protein turnover. This report adds to a modest list of bacterial enzymes apparently regulated by proteolysis, such as GS, MurAA, EnvA, GdhA, and MetA.
    Źródło:
    Acta Biochimica Polonica; 2008, 55, 4; 761-766
    0001-527X
    Pojawia się w:
    Acta Biochimica Polonica
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Response of soybean to inoculation with Bradyhrizobia spp. strains: effect on root nodulation, yield and residual soil nitrogen
    Autorzy:
    Getu, Abebe
    Gashu, Kindu
    Kerebih, Bitewulign
    Wale, Sefinew
    Getaneh, Dessalegn
    Powiązania:
    https://bibliotekanauki.pl/articles/1062932.pdf
    Data publikacji:
    2019
    Wydawca:
    Przedsiębiorstwo Wydawnictw Naukowych Darwin / Scientific Publishing House DARWIN
    Tematy:
    Bradyrhizobium japonicum
    Glycine max
    Jabitehnan
    bradyrhizobia
    fertilizer
    inoculation
    soybean
    strain
    Opis:
    Soybean (Glycine max (L.) Merrill) is an exotic crop to Ethiopia. Hence, inoculation with effective Bradyrhizobia spp. is crucial to improve its productivity. This study was therefore conducted in West Gojam Zone of Amhara Region in 2015 and 2016 aimed at evaluating the effect of bradyrhizobia strains (MAR-1495, SB-12 and TAL-379) with and without phosphorus fertilizer (23 kg P2O5 ha-1) on root nodulation and yield of soybean, and on residual soil nitrogen. The treatments were laid in randomized complete block design including a satellite control treatment with three replications. Effect of the use of rhizobia strains and P fertilizer on nodulation, yield and yield components of soybean was found statistically significant (P<0.05). The highest grain and dry matter yields of 2.7 and 6.4 t ha-1, respectively, were obtained from MAR-1495+P which was statistically at par with the yields obtained from MAR-1495 alone. A grain yield advantage of 30.8% over the control treatment was found from the use of MAR-1495 alone. Similarly, the maximum number of effective nodules (14.9) per plant was recorded from MAR-1495+P which was statistically at par with the number of effective nodules (11.6) per plant counted from MAR-1495 inoculated treatment. The highest residual soil N exceeding the control treatment by 0.029% (0.29 g total N per kg soil) was obtained from MAR-1495 alone. Therefore, inoculation with the strain MAR-1495 was found the most effective for improved soybean production in the study district and similar agroecologies.
    Źródło:
    World Scientific News; 2019, 124, 2; 94-106
    2392-2192
    Pojawia się w:
    World Scientific News
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Straight and branched (ω-1)-hydroxylated very long chain fatty acids are components of Bradyrhizobium lipid A
    Autorzy:
    Choma, Adam
    Komaniecka, Iwona
    Powiązania:
    https://bibliotekanauki.pl/articles/1039947.pdf
    Data publikacji:
    2011
    Wydawca:
    Polskie Towarzystwo Biochemiczne
    Tematy:
    symbiosis
    fatty acids
    LPS
    Bradyrhizobium
    lipid A
    Opis:
    Lipopolysaccharides of seven Bradyrhizobium strains and three whole-cell fatty acid preparations from bacteria isolated from nodules of Sarothamnus scoparius (Common Broom) were studied for the presence of very long chain (ω-1)-hydroxy fatty acids. Several such fatty acids were identified. Among them, straight-chain as well as mono- and dimethyl branched acids with chains in the range from 26 to 34 carbon atoms were found. Pyrrolidides and 4,4-dimethyloxazoline derivatives were used to determine the branching position. Carbons at the (ω-10) and/or (ω-11) positions in alkyl chains were points of attachment of methyl groups. These data complete the structure of bradyrhizobial lipid A with important details. The obtained results can be applied in the chemotaxonomy of Bradyrhizobium.
    Źródło:
    Acta Biochimica Polonica; 2011, 58, 1; 51-58
    0001-527X
    Pojawia się w:
    Acta Biochimica Polonica
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Survival of rhizobia on seeds, nodulation and growth of soybean as influenced by synthetic and natural seed-applied fungicides
    Autorzy:
    Martyniuk, Stefan
    Kozieł, Monika
    Gałązka, Anna
    Powiązania:
    https://bibliotekanauki.pl/articles/2168304.pdf
    Data publikacji:
    2016-12
    Wydawca:
    Instytut Uprawy Nawożenia i Gleboznawstwa – Państwowy Instytut Badawczy
    Tematy:
    oybean
    carboxin
    thiuram
    tea tree oil
    seed dressings
    Bradyrhizobium japonicum
    nodulation
    Opis:
    Soybean seeds (cv. Aldana) were treated with a synthetic seed dressing composed of carboxin (20%) and thiuram (20%) as the active ingredients or with a natural fungicidal preparation containing 23.8% of natural oil extracted from tea tree, and next day untreated (control) and treated with the above mentioned preparations soybean seeds were pelleted with inoculant containing Bradyrhizobium japonicum – the symbiotic bacterium of this plant. Shortly (within 1–2 hours) after seed inoculation and after 24 hours of storing the inoculated seeds at room temperature seed samples were collected to count rhizobial numbers on seeds. At the same sampling times seeds were also sown into pots filled with perlite moistened with water solution of plant nutrients (without N). After 4 weeks of plant growth in a growth chamber numbers of root nodules were counted and fresh and dry mass of shoots were determined. The studied chemical seed dress- ings had no significant effects on numbers of soybean rhizobia on seeds, and on nodulation and growth of soybean plants when seeds were analyzed or planted shortly after their inoculation with B. japonicum. However, the studied preparations significantly reduced numbers of soybean rhizobia on seeds stored for 24 h at room temperature, but only the synthetic one affected adversely nodulation and some growth parameters of soybean plants that developed from the stored seeds
    Źródło:
    Polish Journal of Agronomy; 2016, 27; 96-99
    2081-2787
    Pojawia się w:
    Polish Journal of Agronomy
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
      Wyświetlanie 1-6 z 6

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