Informacja

Drogi użytkowniku, aplikacja do prawidłowego działania wymaga obsługi JavaScript. Proszę włącz obsługę JavaScript w Twojej przeglądarce.

English (EN)·Polski (PL)·Yкраїнський (UK)
Przejdź do strony domowej biblioteki Centralna Biblioteka Wojskowa Link otwiera się w nowym oknie Logo biblioteki Prolib Integro - strona głównaCentralna Biblioteka Wojskowa

Wyszukujesz frazę "Atomic Force Microscopy" wg kryterium: Temat

  Lista filtrów
Wyrównaj
Wyświetlanie 1-25 z 63
Tytuł:
Ion Beam Induced Surface Modification of ta-C Thin Films
Autorzy:
Berova, M.
Sandulov, M.
Tsvetkova, T.
Kitova, S.
Bischoff, L.
Boettger, R.
Powiązania:
https://bibliotekanauki.pl/articles/1033772.pdf
Data publikacji:
2017-08
Wydawca:
Polska Akademia Nauk. Instytut Fizyki PAN
Tematy:
carbon
ion implantation
atomic force microscopy
Opis:
Thin film samples (d ≈40 nm) of tetrahedral amorphous carbon (ta-C), deposited by filtered cathodic vacuum arc, were implanted with Ga⁺ at ion energy E =20 keV and ion fluences D=3×10¹⁴-3×10¹⁵ cm¯² and N⁺ with the same energy and ion fluence D=3×10¹⁴ cm¯². The Ga⁺ ion beam induced surface structural modification of the implanted material, displayed by formation of new phase at non-equilibrium condition, which could be accompanied by considerable changes in the optical properties of the ta-C films. The N⁺ implantation also results in modification of the surface structure. The induced structural modification of the implanted material results in a considerable change of its topography and optical properties. Nanoscale topography and structural properties characterisation of the Ga⁺ and N⁺ implanted films were performed using atomic spectroscopy analysis. The observed considerable surface structural properties modification in the case of the higher fluence Ga⁺ implanted samples results from the relatively high concentration of introduced Ga⁺ atoms, which is of the order of those for the host element.
Źródło:
Acta Physica Polonica A; 2017, 132, 2; 299-301
0587-4246
1898-794X
Pojawia się w:
Acta Physica Polonica A
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
The impact of the light exposure on the morphological properties of selected photoresists
Autorzy:
Sikora, Andrzej
Janus, Paweł
Sierakowski, Andrzej
Powiązania:
https://bibliotekanauki.pl/articles/174640.pdf
Data publikacji:
2019
Wydawca:
Politechnika Wrocławska. Oficyna Wydawnicza Politechniki Wrocławskiej
Tematy:
photolitography
polymer degradation
atomic force microscopy
Opis:
In this paper we present the investigation aimed at the photoresist roughness change determination as a reliable estimator of the exposition rate in the processing verification in semiconductor industry. By employing atomic force microscopy as the 3D high resolution surface imaging tool, we tested twelve popular photoresists in terms of the morphological properties changes, while the following radiation doses were applied. Basing on high precision, and repetitive sample positioning, it was possible to perform the tests with high degree of confidence and observe the roughness change dynamics. Various profiles of roughness changes were observed, showing the need for individual study of each material. Moreover, it was possible to select the photoresists which due to poor homogeneity and small roughness changes are not suitable to such a verification. According to our knowledge, no such study was performed so far.
Źródło:
Optica Applicata; 2019, 49, 1; 177-185
0078-5466
1899-7015
Pojawia się w:
Optica Applicata
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Diffuse-layer surface potentials of colemanites mined in Turkey
Autorzy:
Senol-Arslan, Dilek
Drelich, Jaroslaw W.
Powiązania:
https://bibliotekanauki.pl/articles/2146938.pdf
Data publikacji:
2022
Wydawca:
Politechnika Wrocławska. Oficyna Wydawnicza Politechniki Wrocławskiej
Tematy:
colemanite
surface potential
atomic force microscopy
boron
DLVO
Opis:
Colemanite crystal specimens were handpicked at Kestelek, Emet (Hisarcik, Espey), Bigadic mines in Turkey for characterization of their composition and surface potential. X-ray diffraction analysis revealed no differences in mineralogical makeup of the crystals, but elemental analysis indicated differences in the type of trace (<0.1 wt%) elements. Zeta potential measurements showed small differences in zeta potential values, with isoelectric points (iep) varying from about pH = 9.6 to pH = 10.2. However, no correlation was found between iep and the type of trace elements. Additionally, atomic force microscopy (AFM) was employed to measure the colloidal interactions between a silicon nitride (Si3N4) cantilever tip and colemanite crystal surfaces in 1 wt% colemanite-saturated aqueous solutions at three different pHs (8.4, 9.4 and 11). The Derjaguin-Landau-Verwey-Overbeek theory (DLVO) was applied to examine the AFM tip interactions with colemanite surfaces in an aqueous solution of colemanite saturated solutions. The results revealed attractive forces at pH = 8.4 and 9.4 and repulsive forces at pH = 11, confirming the location of an isoelectric point for colemanite specimens somewhere between pH = 9.5 and pH = 10.1. Theoretical analysis of the force curves using the DLVO theory allowed for assessment of both surface charge density and surface potential for colemanite specimens used in this study.
Źródło:
Physicochemical Problems of Mineral Processing; 2022, 58, 5; art. no. 151933
1643-1049
2084-4735
Pojawia się w:
Physicochemical Problems of Mineral Processing
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
The effect of Galleria mellonella hemolymph polypeptides on Legionella gormanii
Autorzy:
Chmiel, Elżbieta
Palusinska-Szysz, Marta
Zdybicka-Barabas, Agnieszka
Cytryńska, Małgorzata
Mak, Paweł
Powiązania:
https://bibliotekanauki.pl/articles/1039350.pdf
Data publikacji:
2014
Wydawca:
Polskie Towarzystwo Biochemiczne
Tematy:
Legionella gormanii
Galleria mellonella
apolipophorin III
Atomic Force Microscopy
Opis:
Among Legionella species, which are recognized to be pathogenic for humans, L. gormanii is the second prevalent causative agent of community-acquired pneumonia after L. pneumophila. Anti-L. gormanii activity of Galleria mellonella hemolymph extract and apolipophorin III (apoLp-III) was examined. The extract and apoLp-III at the concentration 0.025 mg/ml caused 75% and 10% decrease of the bacteria survival rate, respectively. The apoLp-III-induced changes of the bacteria cell surface were analyzed for the first time by atomic force microscopy. Our studies demonstrated the powerful anti-Legionella effects of the insect defence polypeptides, which could be exploited in drugs design against these pathogens.
Źródło:
Acta Biochimica Polonica; 2014, 61, 1; 123-127
0001-527X
Pojawia się w:
Acta Biochimica Polonica
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Enhancing capabilities of Atomic Force Microscopy by tip motion harmonics analysis
Autorzy:
Babicz, S.
Smulko, J.
Zieliński, A.
Powiązania:
https://bibliotekanauki.pl/articles/199918.pdf
Data publikacji:
2013
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
atomic force microscopy (AFM)
harmonics
van der Waals forces
Opis:
Motion of a tip used in an atomic force microscope can be described by the Lennard-Jones potential, approximated by the van der Waals force in a long-range interaction. Here we present a general framework of approximation of the tip motion by adding three terms of Taylor series what results in non-zero harmonics in an output signal. We have worked out a measurement system which allows recording of an excitation tip signal and its non-linear response. The first studies of spectrum showed that presence of the second and the third harmonics in cantilever vibrations may be observed and used as a new method of the investigated samples characterization.
Źródło:
Bulletin of the Polish Academy of Sciences. Technical Sciences; 2013, 61, 2; 535-539
0239-7528
Pojawia się w:
Bulletin of the Polish Academy of Sciences. Technical Sciences
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Structural Aging and Degradation of Human Fingernail Plates Upon Cosmetic Agents
Autorzy:
Kulesza, S.
Bramowicz, M.
Gwoździk, M.
Wilczyński, S.
Goździejewska, A. M.
Powiązania:
https://bibliotekanauki.pl/articles/354662.pdf
Data publikacji:
2019
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
Atomic Force Microscopy
Scanning Electron Microscopy
fractal characterization
nanoscale property mapping
Opis:
The knowledge whether and how chemical species react with tissues is important because of protection against harmful factors, diagnose of dermatological diseases, validation of dermatological procedures as well as effectiveness of topical therapies. In presented work the effects of chemical agents on plates of human fingernails were studied using Atomic Force Microscopy and Scanning Electron Microscopy. Apart from that, mapping of the elastic properties of the nails was also carried out. To obtain reliable measures of spatial evolution of the surface variations, recorded images were analyzed in terms of scaling invariance brought by fractal geometry, instead of common though not unique statistical measures.
Źródło:
Archives of Metallurgy and Materials; 2019, 64, 1; 181-184
1733-3490
Pojawia się w:
Archives of Metallurgy and Materials
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Microscopic analysis of the nanostructures impact on endothelial cells
Autorzy:
Kołodziejczyk, Agnieszka Maria
Kucińska, Magdalena
Jakubowska, Aleksandra
Siatkowska, Małgorzata
Sokołowska, Paulina
Kotarba, Sylwia
Makowski, Krzysztof
Komorowski, Piotr
Walkowiak, Bogdan
Powiązania:
https://bibliotekanauki.pl/articles/1844983.pdf
Data publikacji:
2020
Wydawca:
Akademia Górniczo-Hutnicza im. Stanisława Staszica w Krakowie. Polskie Towarzystwo Biominerałów
Tematy:
nanostructures
atomic force microscopy
transmission electron microscopy
scanning electron microscopy
cell morphology
Opis:
Nowadays nanostructures are more and more often designed as carriers for drug delivery, especially to improve the drug pharmacokinetics and pharmaco-dynamics. Numerous kinds of nanostructures are considered a good prospect for medical applications thanks to their small size, acceptable biocompatibility and toxicity. Due to the fact that nanotechnology is a new field of science, every nano-scale product must be thoroughly examined regarding its toxicity to the human body. This study provides new insights into effects of exposing endothelial cells to the selected nanostructures. Dendrimers of the fourth generation (PAMAMs), multi-walled carbon nanotubes (MWCNTs) and silver nanoparticles (SNPs) were used to evaluate nanostructures influence on endothelial cells in vitro. The nanostructures were evaluated via transmission electron microscopy and dynamic light scattering technique. The cells previously exposed to the nanostructures were observed and analyzed via the atomic force microscopy and scanning electron microscopy to obtain a quantitative evaluation of the cells morphology. The presence of multi-walled carbon nanotubes and silver nanoparticles on the cells surface was confirmed by the scanning electron microscopy. Our results confirm that the surface association and/or uptake of nanostructures by the cells resulting from physicochemical and biological processes, affect the cells morphology. Morphological changes can be induced by the membrane proteins interaction with nanomaterials, which trigger a sequence of intracel-lular biological processes.
Źródło:
Engineering of Biomaterials; 2020, 23, 154; 2-8
1429-7248
Pojawia się w:
Engineering of Biomaterials
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Microscopic analysis of the nanostructures impact on endothelial cells
Autorzy:
Kołodziejczyk, Agnieszka Maria
Kucińska, Magdalena
Jakubowska, Aleksandra
Siatkowska, Małgorzata
Sokołowska, Paulina
Kotarba, Sylwia
Makowski, Krzysztof
Komorowski, Piotr
Walkowiak, Bogdan
Powiązania:
https://bibliotekanauki.pl/articles/970974.pdf
Data publikacji:
2020
Wydawca:
Akademia Górniczo-Hutnicza im. Stanisława Staszica w Krakowie. Polskie Towarzystwo Biominerałów
Tematy:
nanostructures
atomic force microscopy
transmission electron microscopy
scanning electron microscopy
cell morphology
Opis:
Nowadays nanostructures are more and more often designed as carriers for drug delivery, especially to improve the drug pharmacokinetics and pharmaco-dynamics. Numerous kinds of nanostructures are considered a good prospect for medical applications thanks to their small size, acceptable biocompatibility and toxicity. Due to the fact that nanotechnology is a new field of science, every nano-scale product must be thoroughly examined regarding its toxicity to the human body. This study provides new insights into effects of exposing endothelial cells to the selected nanostructures. Dendrimers of the fourth generation (PAMAMs), multi-walled carbon nanotubes (MWCNTs) and silver nanoparticles (SNPs) were used to evaluate nanostructures influence on endothelial cells in vitro. The nanostructures were evaluated via transmission electron microscopy and dynamic light scattering technique. The cells previously exposed to the nanostructures were observed and analyzed via the atomic force microscopy and scanning electron microscopy to obtain a quantitative evaluation of the cells morphology. The presence of multi-walled carbon nanotubes and silver nanoparticles on the cells surface was confirmed by the scanning electron microscopy. Our results confirm that the surface association and/or uptake of nanostructures by the cells resulting from physicochemical and biological processes, affect the cells morphology. Morphological changes can be induced by the membrane proteins interaction with nanomaterials, which trigger a sequence of intracel-lular biological processes.
Źródło:
Engineering of Biomaterials; 2020, 23, 154; 2-8
1429-7248
Pojawia się w:
Engineering of Biomaterials
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Development and utilization of the nanomarkers for precise AFM tip positioning in the investigation of the surface morphology change
Autorzy:
Sikora, A
Powiązania:
https://bibliotekanauki.pl/articles/174750.pdf
Data publikacji:
2013
Wydawca:
Politechnika Wrocławska. Oficyna Wydawnicza Politechniki Wrocławskiej
Tematy:
atomic force microscopy (AFM)
material science
environmental tests
nanolithography
nanomarker
Opis:
The investigation of the surface properties changes at micrometer and nanometer scale, due to the presence of various factors such as: temperature, solar radiation or magnetic field, requires suitable diagnostic methods. Atomic force microscopy (AFM) is one of the most popular measurement techniques providing necessary resolution. As complex experiments may require multiple moving of the sample between instruments and AFM, one can find quantitative comparison of the results unreliable when the measurements are performed without precise positioning of investigated surface and different areas are analyzed. In this work, the utilization of the nanoscratching method in terms of development of the nanomarkers set is presented, as the solution for precise positioning of the sample in order to perform the multi-step imaging of small surface area (1 μm×1 μm). Various materials were used to verify the versatility of the developed method. Also, the observation of the influence of the UV radiation on the polycarbonate sample was demonstrated as the example proving the application potential of the approach.
Źródło:
Optica Applicata; 2013, 43, 1; 163-171
0078-5466
1899-7015
Pojawia się w:
Optica Applicata
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Piezoresistive sensors for atomic force microscopy - numerical simulations by means of virtual wafer fab
Autorzy:
Dębski, T.
Barth, W.
Rangelow, I.W.
Domański, K.
Tomaszewski, D.
Grabiec, P.
Jakubowski, A.
Powiązania:
https://bibliotekanauki.pl/articles/307644.pdf
Data publikacji:
2001
Wydawca:
Instytut Łączności - Państwowy Instytut Badawczy
Tematy:
atomic force microscopy (AFM)
piezoresistive sensors
technology simulation
technology characterization
Opis:
An important element in microelectronics is the comparison of the modelling and measurements results of the real semiconductor devices. Our paper describes the final results of numerical simulation of a micromechanical process sequence of the atomic force microscopy (AFM) sensors. They were obtained using the virtual wafer fab (VWF) software, which is used in the Institute of Electron Technology (IET). The technology mentioned above is used for fabrication of the AFM cantilevers, which has been designed for measurement and characterization of the surface roughness, the texturing, the grain size and the hardness. The simulation are very useful in manufacturing other microcantilever sensors.
Źródło:
Journal of Telecommunications and Information Technology; 2001, 1; 35-39
1509-4553
1899-8852
Pojawia się w:
Journal of Telecommunications and Information Technology
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Nanoindentation and atomic force microscopy of thin surface layers formed on the shaft surface after burnishing process
Autorzy:
Kuznetsova, T.
Zubar, T.
Chizik, S.
Miszczak, A.
Labuda, W.
Powiązania:
https://bibliotekanauki.pl/articles/242839.pdf
Data publikacji:
2016
Wydawca:
Instytut Techniczny Wojsk Lotniczych
Tematy:
microstructure
atomic force microscopy
nanoindentation
microhardness
Young's modulus
burnishing process
Opis:
The article presents the experimental results of investigation of the pumps shaft surface made of stainless steel after burnishing process. The process of burnishing shafts proposed here aims at increasing the service durability of marine pump shafts of seawater installations, which should give economic benefits in comparison with traditional methods. Burnishing process enables the achievement of high smoothness of machined surface together with the surface layer hardening. This process has been performed in industrial experience on universal machine tools and on CNC machines but it is regarded as plastic tooling. Therefore, the final formation of dimensions and service properties with the use of burnishing constitutes a chipless and dustless treatment, which allows for ranking burnishing among ecological tooling methods. The properties of the surface layers determine the tribological properties of the shaft. The methods of scanning electron microscopy (SEM), atomic force microscopy (AFM) and nanoindentation (NI) were used. The morphology of the changed layers formed by plastic deforming of steel during burnishing treatment was investigated on micro- and nanolevel by SEM and AFM after 1, 2, 3 and 4 passed of burnishing tool. The values of microhardness and Young´s modulus were measured on the shaft cross section from the surface till the 50 – 300 μm depth. The presence of the soft changed layer on the hardened shaft surface after burnishing process determines the decrease of the coefficient of friction during exploitation.
Źródło:
Journal of KONES; 2016, 23, 3; 303-310
1231-4005
2354-0133
Pojawia się w:
Journal of KONES
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Evaluation of Differences between Fe₃O₄ Micro- and Nanoparticles Properties
Autorzy:
Duriagina, Z.
Tepla, T.
Kulyk, V.
Powiązania:
https://bibliotekanauki.pl/articles/1030923.pdf
Data publikacji:
2018-04
Wydawca:
Polska Akademia Nauk. Instytut Fizyki PAN
Tematy:
Fe₃O₄ micro- and nanoparticles
atomic-force microscopy
magnetization
superparamagnetism
Opis:
Small sizes of nanoparticles lead to the appearance of new unique functional properties. Under transition to nanosizes in metals and their compounds new specific characteristics appears. In this work, the microstructural and magnetic properties of Fe₃O₄ nanoparticles (Fe₃O₄-NP) have been compared with those of commercially available Fe₃O₄ microparticles (Fe₃O₄-MP) and detailed analysis of differences has been carried out. The synthesis of Fe₃O₄-NP was carried out by means of colloidal method performed without the use of surfactants. Commercial and synthesized particles were characterized using NTEGRA Prima (NT-MDT) atomic force microscope. For magnetic properties investigations we used the method of vibrating sample magnetometer.
Źródło:
Acta Physica Polonica A; 2018, 133, 4; 869-872
0587-4246
1898-794X
Pojawia się w:
Acta Physica Polonica A
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Atomic force microscopy studies of the adhesive properties of DPPC vesicles containing β-carotene
Autorzy:
Augustyńska, Dominika
Jemioła-Rzemińska, Małgorzata
Burda, Kvetoslava
Strzałka, Kazimierz
Powiązania:
https://bibliotekanauki.pl/articles/1039795.pdf
Data publikacji:
2012
Wydawca:
Polskie Towarzystwo Biochemiczne
Tematy:
atomic force microscopy
dipalmitoylphosphatidylcholine
β-carotene
liposome adhesion
thermal transition
Opis:
A role of carotenoids as modulators of physical properties of model and biological membranes has been already postulated. However, there is a lack of information on the influence of these pigments on interactions between the lipids which form such membranes. This paper applies atomic force microscopy (AFM) in to study the effects of β-carotene on the adhesion properties of DPPC multilamellar liposomes. This allowed us to gain, for the first time, a direct insight into the interactions between the components in model systems on a molecular level. We observe that the adhesive forces in DPPC multilamellar liposomes containing 1mol% of β-carotene decrease exponentially with increasing temperature, and that at about 37°C they diminish. In the case of pure liposomes the decline in adhesion is of a different nature and the adhesive forces disappear at 34°C. The adhesive forces are about 5 times higher at 31°C in the presence of β-carotene than in its absence. However, measurements using differential scanning calorimetry (DSC) showed a shift of the lamellar-to-undulled-lamellar phase transition toward lower temperatures by about 0.8±0.2°C in a system containing β-carotene. The enthalpy changes (ΔH) of this transition are similar for both systems. For the main transition, gel-to-liquid crystalline, the peak is shifted by about 0.5±0.1°C, and ΔH decreases by about 30% in liposomes treated with β-carotene in comparison to pure liposomes. Our results suggest increased cooperation between liposome components in a system with enriched β-carotene, which cause a change in phase transition temperatures. Moreover, these interactions are very sensitive to temperature.
Źródło:
Acta Biochimica Polonica; 2012, 59, 1; 125-128
0001-527X
Pojawia się w:
Acta Biochimica Polonica
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Dynamic force measurements of avidin-biotin and streptavdin-biotin interactions using AFM
Autorzy:
de Odrowąż Piramowicz, Marzena
Czuba, Paweł
Targosz, Marta
Burda, Kvĕtoslava
Szymoński, Marek
Powiązania:
https://bibliotekanauki.pl/articles/1041274.pdf
Data publikacji:
2006
Wydawca:
Polskie Towarzystwo Biochemiczne
Tematy:
atomic force microscopy
rupture force
loading rate
streptavidin
avidin
biotin
dissociation rate
Opis:
Using atomic force microscopy (AFM) we performed dynamic force measurements of the adhesive forces in two model systems: avidin-biotin and streptavidin-biotin. In our experiments we used glutaraldehyde for immobilization of (strept)avidin on the tip and biotin on the sample surface. Such interface layers are more rigid than those usually reported in the literature for AFM studies, when (strept)avidin is coupled with biotinylated bovine albumin and biotin with agarose polymers. We determined the dependence of the rupture forces of avidin-biotin and streptavidin-biotin bonds in the range 300-9600 pN/s. The slope of a semilogarithmic plot of this relation changes at about 1700 pN/s. The existence of two different regimes indicates the presence of two activation barriers of these complexes during the dissociation process. The dissociation rates and activation energy barriers, calculated from the Bell model, for the avidin-biotin and streptavidin-biotin interactions are similar to each other for loading rates >1700 pN/s but they are different from each other for loading rates < 1700 pN/s. In the latter case, the dissociation rates show a higher stability of the avidin-biotin complex than the streptavidin-biotin complex due to a larger outer activation barrier of 0.8 kBT. The bond-rupture force is about 20 pN higher for the avidin-biotin pair than for the streptavidin-biotin pair for loading rates < 1700 pN/s. These two experimental observations are in agreement with the known structural differences between the biotin binding pocket of avidin and of streptavidin.
Źródło:
Acta Biochimica Polonica; 2006, 53, 1; 93-100
0001-527X
Pojawia się w:
Acta Biochimica Polonica
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Cartilage tissue examination using atomic force microscopy
Autorzy:
Paluch, Jarosław
Markowski, Jarosław
Pilch, Jan
Smółka, Wojciech
Jasik, Krzysztof Piotr
Kilian, Filip
Likus, Wirginia
Bajor, Grzegorz
Chrobak, Dariusz
Glowka, Karsten
Starczewska, Oliwia
Powiązania:
https://bibliotekanauki.pl/articles/27324036.pdf
Data publikacji:
2022
Wydawca:
Akademia Górniczo-Hutnicza im. Stanisława Staszica w Krakowie. Polskie Towarzystwo Biominerałów
Tematy:
atomic force microscopy
cartilage
biopolymers
chondrocytes
intercellular matrix
mikroskop
biopolimery
tkanka
Opis:
Life sciences, a field closely intertwined with human biology and physiology, employ various research methods, including morphology studies and quantitative analysis through non-destructive techniques. Biological specimens often consist of three-phase structures, characterized by the presence of gas, liquid, and solid components. This becomes crucial when the chosen research methodology requires the removal of water from samples or their transfer to a cryostat. In the current research, mechanical and topographical examination of cartilage was performed. The materials were generously provided by the Department of Anatomy at the Medical University of Silesia, thereby eliminating any concerns regarding their origin or ethical use for scientific purposes. Our research methodology involved the application of atomic force microscopy (AFM), which minimally disrupts the internal equilibrium among the aforementioned phases. Cartilage, recognized as a ‘universal support material’ in animals, proves to be highly amenable to AFM research, enabling the surface scanning of the examined material. The quantitative results obtained facilitate an assessment of the internal structure and differentiation of cartilage based on its anatomical location (e.g., joints or ears). Direct images acquired during the examination offer insights into the internal structure of cartilage tissue, revealing morphological disparities and variations in intercellular spaces. The scans obtained during the measurements have unveiled substantial distinctions, particularly in the intercellular ‘essence’, characterized by granularities with a diameter of approximately 0.5 μm in ear cartilage and structural elements in articular cartilage measuring about 0.05 μm. Thus, AFM can be a valuable cognitive tool for observing biological samples in the biological sciences, particularly in medicine (e.g. clinical science).
Źródło:
Engineering of Biomaterials; 2022, 25, 167; 17--23
1429-7248
Pojawia się w:
Engineering of Biomaterials
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Application of epoxy functional silanes in the preparation of DNA microarrays
Autorzy:
Frydrych-Tomczak, E.
Uszczynska, B.
Ratajczak, T.
Markiewicz, W.T.
Figlerowicz, M.
Nowicki, M.
Maciejewski, H.
Chmielewski, M.K.
Powiązania:
https://bibliotekanauki.pl/articles/80900.pdf
Data publikacji:
2014
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
DNA microarray
gene expression
organofunctional silane
oligonucleotide
atomic force microscopy
goniometer
Źródło:
BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology; 2014, 95, 1
0860-7796
Pojawia się w:
BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
The study of harmonic imaging by AFM
Badania harmonicznych w obrazowaniu AFM
Autorzy:
Babicz, S.
Powiązania:
https://bibliotekanauki.pl/articles/156072.pdf
Data publikacji:
2011
Wydawca:
Stowarzyszenie Inżynierów i Techników Mechaników Polskich
Tematy:
mikroskop sił atomowych
powierzchnia
harmoniczne
atomic force microscopy (AFM)
surface
harmonics
Opis:
Atomic Force Microscopy (AFM) is a powerful tool for the analysis of surface samples with accuracy of single atoms. The existing methods include surface roughness, porosity and hardness of the test portion of the sample. The article presents the preliminary studyof a new AFM method of surface analysis. The study indicates that there may be a correlation between intensity of a harmonic resonance frequency of the needle and the system response. The suggested correlation can characterize elasticity of the analyzed surface.
Mikroskop sił atomowych (ang. Atomic Force Microscope - AFM) został wynaleziony w 1986 roku [1] jako alternatywa dla skaningowego mikroskopu tunelowego (ang. Scanning Tunneling Microscope - STM), którego nie można użyć do badań nad materiałami nieprzewodzącymi. AFM umożliwia pomiary materiałów zanurzonych w cieczach, co pozwala badać żywe preparaty biologiczne w warunkach zbliżonych do ich naturalnego środowiska [2]. W artykule przedstawiono zasadę pracy mikroskopu (rys. 1) oddziaływującego siłami van der Waalsa (opisanymi funkcją Lennarda - Jonesa) między ostrzem skanującym a próbką (1) (rys. 2) [3]. Opisano trzy podstawowe tryby pracy mikroskopu: kontaktowy, przerywany [4-6] oraz bezkontaktowy (2). Opierając się na dotychczasowych badaniach [7] wyznaczających różne właściwości materiału w zależności od ich budowy (rys. 3, rys. 4) przebadano próbkę warystora (rys. 5) pod kątem obecności i poziomu kolejnych harmonicznych pobudzającej częstotliwości rezonansowej w odpowiedzi układu. Przeprowadzone pomiary wskazują, że może istnieć związek między intensywnością kolejnych harmonicznych, a właściwościami badanej powierzchni.
Źródło:
Pomiary Automatyka Kontrola; 2011, R. 57, nr 12, 12; 1508-1510
0032-4140
Pojawia się w:
Pomiary Automatyka Kontrola
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
A Comparison between Contact and Tapping AFM Mode in Surface Morphology Studies
Autorzy:
Bramowicz, M.
Kulesza, S.
Rychlik, K.
Powiązania:
https://bibliotekanauki.pl/articles/298112.pdf
Data publikacji:
2012
Wydawca:
Uniwersytet Warmińsko-Mazurski w Olsztynie
Tematy:
mikroskopia sił atomowych
AFM
topografia powierzchni
atomic force microscopy (AFM)
surface topography
Opis:
The paper presents recent results from studies of a surface topography of a platinum calibration grid on silicon substrate obtained in both contact and tapping modes of the AFM microscope. The results are analyzed in order to determine the influence of the scan set-up and the SPM probe onto estimated fractal parameters and surface anisotropy ratio.
Źródło:
Technical Sciences / University of Warmia and Mazury in Olsztyn; 2012, 15(2); 307-318
1505-4675
2083-4527
Pojawia się w:
Technical Sciences / University of Warmia and Mazury in Olsztyn
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Multiscale water drop contact angles at selected silica surfaces
Autorzy:
Zhang, Chen
Wang, Xuming
Li, Lixia
Jin, Jiaqi
Polson, Randy
Miller, Jan D.
Powiązania:
https://bibliotekanauki.pl/articles/2146924.pdf
Data publikacji:
2022
Wydawca:
Politechnika Wrocławska. Oficyna Wydawnicza Politechniki Wrocławskiej
Tematy:
contact angle
atomic force microscopy
hollow tip
submicron-drop
wettability
MD simulation
Opis:
In this study, multiscale advancing contact angles for glycerol/water drops at silica surfaces are reported for millidrops, submicron-drops, and nanodrops. Selected silica surfaces were muscovite, silicon, and talc. The contact angles for millidrops (1–2 mm) were determined by the traditional sessile drop technique. For submicron-drops (0.1–1.0 μm), a hollow tip Atomic Force Microscope (AFM) procedure was used. The contact angles for nanodrops (~7 nm) were examined from Molecular Dynamics (MD) simulation. The results were compared to evaluate the effect of drop size on the contact angle. In the case of the hydrophobic talc surface, the 75° advancing contact angle did not vary significantly with drop size. For the hydrophilic muscovite surface, the water drop wet the surface and an advancing contact angle of about 10° was found for the millidrops and submicron-drops. However, for the MD simulated nanodrops, attachment and spreading of the ~7 nm drop created a 2D film of molecular dimensions, the contact angle of which was difficult to define and varied from 0° to 17°. Perhaps of equal interest from the MD simulation results was that the spreading of the glycerol/water nanodrop at the muscovite surface resulted in crystallographic directional transport of water molecules to the extremities of the 2D film. Such separation and segregation left the center of the film with an increased concentration of glycerol. Based on these results, the line tension, which has been found in other investigations to account for contact angle decrease with a decrease in drop size, does not seem to be a significant factor in this study.
Źródło:
Physicochemical Problems of Mineral Processing; 2022, 58, 5; art. no. 152154
1643-1049
2084-4735
Pojawia się w:
Physicochemical Problems of Mineral Processing
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Insertion of GPI-anchored alkaline phosphatase into supported membranes: a combined AFM and fluorescence microscopy study.
Autorzy:
Rieu, Jean-Paul
Ronzon, Frédéric
Place, Christophe
Dekkiche, Fairouz
Cross, Benjamin
Roux, Bernard
Powiązania:
https://bibliotekanauki.pl/articles/1043341.pdf
Data publikacji:
2004
Wydawca:
Polskie Towarzystwo Biochemiczne
Tematy:
fluorescent beads
atomic force microscopy (AFM)
GPI anchors
alkaline phosphatase
supported lipid membranes
Opis:
A new method based on combined atomic force microscopy (AFM) and fluorescence microscopy observations, is proposed to visualize the insertion of glycosylphosphatidyl inositol (GPI) anchored alkaline phosphatase from buffer solutions into supported phospholipid bilayers. The technique involves the use of 27 nm diameter fluorescent latex beads covalently coupled to the amine groups of proteins. Fluorescence microscopy allows the estimation of the relative protein coverage into the membrane and also introduces a height amplification for the detection of protein/bead complexes with the AFM. The coupling of the beads with the amine groups is not specific; this new and simple approach opens up new ways to investigate proteins into supported membrane systems.
Źródło:
Acta Biochimica Polonica; 2004, 51, 1; 189-197
0001-527X
Pojawia się w:
Acta Biochimica Polonica
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Surface forces in chemical mechanical planarization and semiconductor wafer cleaning systems
Autorzy:
Hupka, Lukasz
Nalaskowski, Jakub
Miller, Jan D.
Hupka, Jan
Powiązania:
https://bibliotekanauki.pl/articles/1853722.pdf
Data publikacji:
2021
Wydawca:
Polskie Towarzystwo Chemiczne
Tematy:
chemical mechanical planarization
CMP
surface chemistry phenomena
colloidal probe
atomic force microscopy
AFM
Opis:
Superior uniformity and local planarity of semiconductor wafers in the chemical mechanical planarization (CMP) process as well as efficient post-CMP cleaning is controlled by surface chemistry phenomena. The AFM colloidal probe technique was used to demonstrate surface forces which are of special significance to CMP and post-CMP cleaning. Examples of ways to manipulate those interactions are provided, and the benefits to CMP processes and post-CMP cleaning are discussed.
Źródło:
Wiadomości Chemiczne; 2021, 75, 9-10; 1229-1240
0043-5104
2300-0295
Pojawia się w:
Wiadomości Chemiczne
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Damage distributions in GaAs single crystal irradiated with 84Kr (394 MeV), 209Bi (710 MeV) and 238U (1300 MeV) swift ions
Autorzy:
Didyk, A. Y.
Komarov, F. F.
Vlasukova, L. A.
Gracheva, E. A.
Hofman, A.
Yuvchenko, V. N.
Wiśniewski, R.
Wilczyńska, T.
Powiązania:
https://bibliotekanauki.pl/articles/146738.pdf
Data publikacji:
2008
Wydawca:
Instytut Chemii i Techniki Jądrowej
Tematy:
semiconductors
gallium arsenide
swift heavy ions
inelastic energy loss
atomic force microscopy (AFM)
Opis:
We are presenting a study of damage distribution in GaAs irradiated with 84Kr ions of energy EKr = 394 MeV up to the fluence of 5 × 1012 ion/cm-2. The distribution of damage along the projected range of 84Kr ions in GaAs was investigated using selective chemical etching of a single crystal cleaved perpendicularly to the irradiated surface. The damage zone located under the Bragg peak of 84Kr ions was observed. Explanation of the observed effects based on possible processes of channeling of knocked target atoms (Ga and As) is proposed.
Źródło:
Nukleonika; 2008, 53, 2; 77-82
0029-5922
1508-5791
Pojawia się w:
Nukleonika
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
The implementation and the performance analysis of the multi-channel software-based lock-in amplifier for the stiffness mapping with atomic force microscope (AFM)
Autorzy:
Sikora, A.
Bednarz, Ł.
Powiązania:
https://bibliotekanauki.pl/articles/201101.pdf
Data publikacji:
2012
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
atomic force microscopy (AFM)
stiffness mapping
lock-in amplifier
software development
torsional oscilators
Opis:
In this paper the implementation of the surface stiffness mapping method with the dynamic measurement mode of atomic force microscopy (AFM) is presented. As the measurement of the higher harmonics of the cantilever’s torsional bending signal is performed, we are able to visualize non-homogeneities of the surface stiffness. In order to provide signal processing with the desired sensitivity and selectivity, the lock-in amplifier-based solution is necessary. Due to the presence of several useful frequencies in the signal, the utilization of several simultaneously processing channels is required. Therefore the eight-channel software-based device was implemented. As the developed solution must be synchronized with the AFM controller during the scanning procedure, the real-time processing regime of the software is essential. We present the results of mapping the surface stiffness and the performance tests results for different working conditions of the developed setup.
Źródło:
Bulletin of the Polish Academy of Sciences. Technical Sciences; 2012, 60, 1; 83-88
0239-7528
Pojawia się w:
Bulletin of the Polish Academy of Sciences. Technical Sciences
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Development of a hybrid Atomic Force microscope and Optical Tweezers apparatus
Autorzy:
Zembrzycki, K.
Pawłowska, S.
Nakielski, P.
Pierini, F.
Powiązania:
https://bibliotekanauki.pl/articles/31343079.pdf
Data publikacji:
2016
Wydawca:
Instytut Podstawowych Problemów Techniki PAN
Tematy:
optical tweezers
atomic force microscopy (AFM)
AFM/OT
mikroskopia sił atomowych
szczypce optyczne
Opis:
The role of mechanical properties is essential to understand molecular, biological materials and nanostructures dynamics and interaction processes. Atomic force microscopy (AFM), due to its sensitivity is the most commonly used method of direct force evaluation. Yet because of its technical limitations this single probe technique is unable to detect forces with femtonewton resolution. In this paper, we present the development of a combined atomic force microscopy and optical tweezers (AFM/OT) instrument. The system is based on a commercial AFM and confocal microscope. The addition of three lasers along with beam shaping and steering optics, on which the optical tweezer is based upon, provide us with the ability to manipulate small dielectric objects suspended in a fluid. Additionally, this same device allows for direct displacement and force measurement with very high resolution and accuracy in the same AFM scanning zone. We have also fitted a laser and a set of filters to observe fluorescent samples appropriately exited. We show that this is a great improvement of a standalone AFM force resolution and more so opens a way to conduct experiments using a hybrid double probe technique with high potential in nanomechanics, molecules manipulation and biological studies. This paper describes in detail the construction of all the modules such as the trapping laser optics, detection laser optics and the fluorescence module. Also, due to its importance on the performance of the equipment, the electronics part of the detection system is described. In the following chapters the whole adjustment and calibration is explained. The performance of the apparatus is fully characterized by studying the ability to trap dielectric objects and quantifying the detectable and applicable forces. The setting and sensitivity of the particle position detector and force sensor is shown. We additionally describe and compare different optical tweezer calibration methods. In the last part we show the ability of our instrument to conduct experiments using the proposed double-probe technique, in this case to study interaction forces between two particles.
Pomiary własności mechanicznych i sił w mikro i nanoskali mają bardzo ważne znaczenie w badaniach dynamiki i oddziaływań materiałów biologicznych i nanostruktur. Mikroskopia sił atomowych (ang. AFM), z uwagi na swoją czułość, jest najczęściej używaną techniką do bezpośredniego pomiaru sił. Jednak z powodu swoich ograniczeń nie jest w stanie mierzyć sił z rozdzielczością w zakresie femtonewtonów. Takie możliwości stwarza metoda optyczna oparta na tzw. szczypcach optycznych (ang. OT). Z drugiej strony z uwagi na fizyczne ograniczenia tej metody pomiary charakteryzujące oddziaływujące powierzchnie w niewielkich odległościach, nadal wymagają stosowania mikroskopii sił atomowych. W poniższej pracy opisujemy unikalną konstrukcję hybrydową bazującą na połączeniu obu technik w jednym systemie. Skonstruowany system (AFM/OT) oparty jest na autorskiej modyfikacji komercyjnego mikroskopu AFM (firmy NT-MDT) i jest możliwy do zastosowania zasadniczo w każdym innym dostępnym na rynku mikroskopie typu AFM. Modyfikacja polega zasadniczo na budowie dodatkowego systemu optycznego formującego wiązki trzech laserów, na których bazują szczypce optyczne. Umożliwia to manipulację małymi obiektami dielektrycznymi zawieszonymi w płynie i precyzyjną detekcję ich położenia. Dzięki takiej modyfikacji stworzony system AFM/OT pozwala na bezpośrednie pomiary przemieszczenia oraz siły z bardzo dużą rozdzielczością i dokładnością w obszarze działania sondy AFM. Dodatkowo system został wyposażony w laser i elementy optyczne pozwalające na pobudzanie i detekcję fluorescencji odpowiednio przygotowanych obiektów. Wykazujemy, że ten instrument istotnie poprawia zakres i rozdzielczość sił mierzonych za pomocą standardowego mikroskopu AFM, jak również otwiera drogę do przeprowadzania eksperymentów z użyciem hybrydowej techniki dwóch sond, mającej wysoki potencjał zastosowań w nanomechanice, badaniach biologicznych i nanomanipulacji. Niniejsza praca przedstawia szczegóły konstrukcyjne zbudowanych modułów szczypiec optycznych, jak i układ elektroniczny pozwalający na precyzyjne pomiary przemieszczeń obiektów uwięzionych przez szczypce optyczne. W kolejnych partiach pracy przedstawiona jest procedura dostrajania układu optycznego i metodyka kalibracji systemu pomiaru sił i przemieszczeń. Podstawowe parametry aparatury zostają w pełni scharakteryzowane poprzez zbadanie jej zdolności do manipulacji dielektrycznymi obiektami oraz do mierzeni wywieranych na nie sił. Dodatkowo przedstawiamy opis i porównanie różnych metod kalibracji szczypiec optycznych. W ostatniej części pracy przedstawiamy na przykładzie pomiaru oddziaływań bliskiego kontaktu dwóch cząstek koloidalnych potencjał naszego instrumentu dla przeprowadzania pomiarów z jednoczesnym użyciem techniki dwóch sond (AFM/OT).
Źródło:
IPPT Reports on Fundamental Technological Research; 2016, 2; 1-58
2299-3657
Pojawia się w:
IPPT Reports on Fundamental Technological Research
Dostawca treści:
Biblioteka Nauki
Artykuł
Wyświetlanie 1-25 z 63

Ta witryna wykorzystuje pliki cookies do przechowywania informacji na Twoim komputerze. Pliki cookies stosujemy w celu świadczenia usług na najwyższym poziomie, w tym w sposób dostosowany do indywidualnych potrzeb. Korzystanie z witryny bez zmiany ustawień dotyczących cookies oznacza, że będą one zamieszczane w Twoim komputerze. W każdym momencie możesz dokonać zmiany ustawień dotyczących cookies