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Tytuł:
Activity of glycogen depolymerizing enzymes in extracts from brain tumor tissue (anaplastic astrocytoma and glioblastoma multiforme).
Autorzy:
Kotoński, Bogusław
Wilczek, Joanna
Madej, Janusz
Zarzycki, Andrzej
Hutny, Jan
Powiązania:
https://bibliotekanauki.pl/articles/1044051.pdf
Data publikacji:
2001
Wydawca:
Polskie Towarzystwo Biochemiczne
Tematy:
glioblastoma
α-amylase
α-glucosidase
phosphorylase
astrocytoma
Opis:
An approximately threefold increase in glycogenolytic activity of the neutral α-1,4-glucosidase and a twofold increase in the same activity of the acid isoform have been found in extracts of anaplastic astrocytoma and glioblastoma multiforme tumors of brain tissue. "Maltase activity" of the respective enzymes increased by 60-80% in both kinds of tumor extracts. However a significant decrease in α-amylase and almost complete disappearance of phosphorylase activities have also been found in both kinds of tumors.
Źródło:
Acta Biochimica Polonica; 2001, 48, 4; 1085-1090
0001-527X
Pojawia się w:
Acta Biochimica Polonica
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Assessment of chemical composition of flour and its effect on baking characteristics of wheat
Autorzy:
Subda, H
Powiązania:
https://bibliotekanauki.pl/articles/1371393.pdf
Data publikacji:
1992
Wydawca:
Instytut Rozrodu Zwierząt i Badań Żywności Polskiej Akademii Nauk w Olsztynie
Tematy:
flour
wheat flour
glutenin
food
gliadin
chemical composition
high-molecular glutenin
proteolytic enzyme
wheat
alpha-amylase
baking
protein
total protein
Źródło:
Polish Journal of Food and Nutrition Sciences; 1992, 01, 2; 15-21
1230-0322
2083-6007
Pojawia się w:
Polish Journal of Food and Nutrition Sciences
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Biochemical indexes of the liver functioning at the acute stage of opisthorchiasis
Autorzy:
Bakshtanovskaya, I.
Stepanova, T.
Shonin, A.
Ponomareva, O.
Terekhina, V.
Powiązania:
https://bibliotekanauki.pl/articles/837329.pdf
Data publikacji:
1998
Wydawca:
Polskie Towarzystwo Parazytologiczne
Tematy:
bilirubin
gamma-glutamyltransferase
glucose
alanine aminotransferase
aspartate aminotransferase
concentration
alkaline phosphatase
opistchorchiasis
alpha-amylase
cholesterol
liver function
serum activity
Źródło:
Annals of Parasitology; 1998, 44, 3
0043-5163
Pojawia się w:
Annals of Parasitology
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Bioethanol Production from Chlorella Pyrenoidosa by Using Enzymatic Hydrolysis and Fermentation Method
Autorzy:
Yerizam, Muhammad
Jannah, Asyeni Miftahul
Aprianti, Nabila
Powiązania:
https://bibliotekanauki.pl/articles/2202327.pdf
Data publikacji:
2023
Wydawca:
Polskie Towarzystwo Inżynierii Ekologicznej
Tematy:
Chlorella pyrenoidosa
hydrolysis
enzyme
alpha-amylase
glucose
fermentation
bioethanol
Opis:
Starch can be found in microalgae, the raw material for the third generation of bioethanol production. One of them is C. pyrenoidosa. This study was conducted to analyze the effect of α-amylase enzyme concentration on the glucose contents produced and the effects of fermentation time on bioethanol contents produced. The hydrolysis process of this study was conducted using an α-amylase enzyme produced by A. niger. Several analyses in this research were carried out, including the analysis of enzyme activity using the Fuwa method, the analysis of glucose levels from enzymatic hydrolysis using the DNS method, and the analysis of bioethanol contents using the density method and GC-MS. The highest glucose content was 0.67 mg/mL, which was obtained from the addition of 40% (v/w) α-amylase enzyme, and the yield of bioethanol content from the sample treated 40% (v/w) α-amylase enzyme and fermented for 9 days was the optimum, which produced 28.07% of bioethanol content.
Źródło:
Journal of Ecological Engineering; 2023, 24, 1; 34--40
2299-8993
Pojawia się w:
Journal of Ecological Engineering
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Content of glycogen and trehalose and activity of alpha-amylase and trehalase in Galleria mellonella larvae infected with entomopathogenic nematodes Steinernema affinis and S. feltiae
Autorzy:
Zoltowska, K
Lopienska-Biernat, E.
Powiązania:
https://bibliotekanauki.pl/articles/839414.pdf
Data publikacji:
2006
Wydawca:
Polskie Towarzystwo Parazytologiczne
Tematy:
Galleria mellonella
Steinernema feltiae
insect host
amylase
trehalase
host
parasite
trehalose
glycogen
parasitology
alpha-amylase
nematode
larva
Steinernema affinis
entomopathogenic nematode
Opis:
Introduction. The influence of infection with two species of entomopathogenic nematodes of Steinernematidae family on metabolism of glycogen and trehalose of the host was studied. Material and methods. Last instar larvae (L₇) of Galleria mellonella were experimentally infected with Steinernema affinis and S. feltiae. At 6, 12, 18 and 24 h after infection concentrations of trehalose and glycogen as well as activity of trehalase and α-amylase were determined. Results. The content of glycogen was lower in insects infected with S. feltiae than in the controls and animals infected with S. affinis. The content of trehalose was higher in insects from both infected groups than in the controls. Its concentration was slightly higher in larvae infected with S. affinis than in those infected with S. feltiae. The activity of α-amylase after infection with S. affinis was low. It was significantly higher in insects infected with S. feltiae. In animals of both infected groups, following a significant reduction at 6 h, the activity of trehalase remained at a similar level, higher than in the controls. In the paper the effects of infection with (i) different species of entomopathogenic nematodes and (ii) the importance of the developmental stage of the insect-host for changes in its metabolism of glycogen and trehalose were discussed.
Źródło:
Annals of Parasitology; 2006, 52, 2
0043-5163
Pojawia się w:
Annals of Parasitology
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Content of glycogen and trehalose and activity of alpha-amylase and trehalase in Galleria mellonella larvae infected with entomopathogenic nematodes Steinernema affinis and S. feltiae
Autorzy:
Żółtowska, K.
Łopieńska-Biernat, E.
Powiązania:
https://bibliotekanauki.pl/articles/2144330.pdf
Data publikacji:
2006
Wydawca:
Polskie Towarzystwo Parazytologiczne
Tematy:
Galleria mellonella
Steinernema feltiae
insect host
amylase
trehalase
host
parasite
trehalose
glycogen
parasitology
alpha-amylase
nematode
larva
Steinernema affinis
entomopathogenic nematode
Opis:
Introduction. The influence of infection with two species of entomopathogenic nematodes of Steinernematidae family on metabolism of glycogen and trehalose of the host was studied. Material and methods. Last instar larvae (L₇) of Galleria mellonella were experimentally infected with Steinernema affinis and S. feltiae. At 6, 12, 18 and 24 h after infection concentrations of trehalose and glycogen as well as activity of trehalase and α-amylase were determined. Results. The content of glycogen was lower in insects infected with S. feltiae than in the controls and animals infected with S. affinis. The content of trehalose was higher in insects from both infected groups than in the controls. Its concentration was slightly higher in larvae infected with S. affinis than in those infected with S. feltiae. The activity of α-amylase after infection with S. affinis was low. It was significantly higher in insects infected with S. feltiae. In animals of both infected groups, following a significant reduction at 6 h, the activity of trehalase remained at a similar level, higher than in the controls. In the paper the effects of infection with (i) different species of entomopathogenic nematodes and (ii) the importance of the developmental stage of the insect-host for changes in its metabolism of glycogen and trehalose were discussed.
Źródło:
Wiadomości Parazytologiczne; 2006, 52, 2; 103-107
0043-5163
Pojawia się w:
Wiadomości Parazytologiczne
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Core structure of flavonoids precursor as an antihyperglycemic and antihyperlipidemic agent: an in vivo study in rats
Autorzy:
Najafian, Mahmoud
Ebrahim-Habibi, Azadeh
Yaghmaei, Parichehreh
Parivar, Kazem
Larijani, Bagher
Powiązania:
https://bibliotekanauki.pl/articles/1040323.pdf
Data publikacji:
2010
Wydawca:
Polskie Towarzystwo Biochemiczne
Tematy:
diabetes
hyperglycemia
trans-chalcone
hyperlipidemia
benzylideneacetophenone
alpha-amylase inhibitor
Opis:
trans-Chalcone is the core structure of naringenin chalcone, located halfway in the biosynthesis pathway of flavonoids. Flavonoids have been reported as mammalian alpha-amylase inhibitors, a property which could be useful in the management of postprandial hyperglycemia in diabetes and related disorders. As a mammalian alpha-amylase inhibitor in vitro, the putative beneficial effect of trans-chalcone on diabetes was tested in a streptozotocin-induced rat model of diabetes type 1, and the results analyzed with commonly used statistical methods. Significant reduction of blood glucose levels and beneficial effect on dyslipidemia were observed in diabetic rats, as well as reduction of disturbing consequences of diabetes such as high urine volume and water intake. trans-chalcone was observed to have a weight loss-inductive effect, alongside with a reduction in food intake, which is suggestive of a therapeutic potential of this compound in overweight and obese patients.
Źródło:
Acta Biochimica Polonica; 2010, 57, 4; 553-560
0001-527X
Pojawia się w:
Acta Biochimica Polonica
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
DIAGNOSTIC POTENTIAL OF SELECTED SALIVARY PROTEOMICS FOR AUTONOMIC NERVOUS SYSTEM ACTIVITY ASSESSMENT
Autorzy:
Dobrek, Lukasz
Powiązania:
https://bibliotekanauki.pl/articles/2137800.pdf
Data publikacji:
2020-05-14
Wydawca:
Fundacja Edukacji Medycznej, Promocji Zdrowia, Sztuki i Kultury Ars Medica
Tematy:
saliva
vasoactive intestinal peptide
neuropeptide Y
chromogranin A
α-amylase
Opis:
The clinical assessment of autonomic nervous system (ANS) functioning, enabling the diagnosis of autonomic neuropathy present in the course of many diseases, is currently based on performing simple cardiovascular reflexes (Ewing tests), analyzing heart rate variability (HRV) or heart rate turbulence (HRT), examining skin sweating or recording neurophysiological tests (e.g. microneurography). Laboratory assessment of ANS function is very scarce and practically only includes the plasma assessment of noradrenaline as a surrogate for the biochemical indicator of sympathetic activity. Recently, the possibility of evaluation of selected compounds present in saliva as laboratory markers of not only oral diseases but also systemic diseases has been raised. This work focuses on a brief description of the anatomy and physiology of the salivary glands and describes the formation of saliva, its composition and the use of this bodily fluid in laboratory diagnostics. In addition, the paper specifically discusses the possibility of determining selected compounds that are considered to reflect autonomic activity. A review of the literature indicates primarily four proteomics: two neuropeptides (vasoactive intestinal peptide (VIP) and neuropeptide Y (NPY) that are co-transmitters in autonomic fibers, chromogranin A, a synaptic vesicle protein and α-amylase, a hydrolytic enzyme pre-digesting carbohydrates in the oral cavity. These are currently the most widely investigated agents for their usefulness as laboratory markers of ANS activity.
Źródło:
Acta Neuropsychologica; 2020, 18(2); 285-303
1730-7503
2084-4298
Pojawia się w:
Acta Neuropsychologica
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
DIPSACUS FULLONUM L. LEAVES AND ROOTS - IDENTIFICATION OF THE COMPONENTS OF THE ESSENTIAL OILS AND ALPHA-AMYLASE INHIBITORY ACTIVITIES OF METHANOLIC EXTRACTS
Autorzy:
Witkowska-Banaszczak, Ewa
Powiązania:
https://bibliotekanauki.pl/articles/895320.pdf
Data publikacji:
2018-08-31
Wydawca:
Polskie Towarzystwo Farmaceutyczne
Tematy:
essential oil composition
methanolic extract
alpha-amylase inhibition
Opis:
Abstract: Dipsacus fullonum L. syn. D. sylvestris is a biennial plant from the Dipsacaceae family. It grows in Europe, North Africa and Asia Minor. It has been used in folk medicine mainly to treat Lyme disease. This study was undertaken to identify and compare the components of the essential oils from dried and fresh roots, which are usually applied as medicinal agents, as well as those of the essential oils from dried and fresh leaves, used less frequently. The essential oils from the Dipsacus fullonum leaves and roots were analysed by the GC and GC-MS analysis. 18 components were identified in the essential oil from the fresh leaves, whereas 37 were recognised in the oil from the dried leaves; 10 components were identified in the essential oil from the fresh roots and 40 in that from the dried roots. Phytol was the main component in the essential oil from the leaves, whereas n-hexadecanoic acid was predominant in the roots. The α -amylase inhibition has been proven for methanolic extracts of D. fullonum leaves and roots. The strongest activity was demonstrated by the extract from fresh leaves of D. fullonum and showed an effect about one hundred times weaker than acarbose.
Źródło:
Acta Poloniae Pharmaceutica - Drug Research; 2018, 75, 4; 951-957
0001-6837
2353-5288
Pojawia się w:
Acta Poloniae Pharmaceutica - Drug Research
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Effect of steeping degree, malting temperature and addition of gibberellic acid on the accumulation of alpha-amylase in malt
Wpływ stopnia namoczenia, temperatury słodowania i dodatku kwasu giberelinowego na nagromadzenie alfa-amylazy w słodzie
Autorzy:
Surmiński, J.
Masior, S.
Kuchciak, T.
Powiązania:
https://bibliotekanauki.pl/articles/1398778.pdf
Data publikacji:
1986
Wydawca:
Instytut Rozrodu Zwierząt i Badań Żywności Polskiej Akademii Nauk w Olsztynie
Tematy:
malting harley
a-amylase
giberellic acid
Opis:
Malting on a microtechnological scale was performed with the use of brewer's barley of Trumpf and Polon varieties and with the feed variety Diva, all from the 1982 crop. The highest α-amylase content was found in the Polon variety; this content in the Trumpf variety was lower by about 7%, while in the feed variety Diva it was lower by about 20%. The following conditions were found to favour the accumulation of α-amylase in malt containing no addition of gibberellic acid (g.a.): malted grain humidity - 46%, malting temperature - 18°C, time of malting - 7 days. When gibberellic acid was added in doses of 0.2-0.3 mg/kg barley and when pH of the water with g.a. ranged from 6.5 to 7.5, the mean increase of α-amylase content in the studied malt varieties was 54% as compared to control samples without g.a. In the case of the variety Diva this increase was 57%. The conditions of malting with an addition of g.a. that were worked out allow the production of brewer's malt from Diva barley.
Do słodowania w skali mikrotechnicznej użyto krajowe odmiany browarne jęczmienia: Trumpf i Polon oraz odmianę paszową Diva ze zbioru 1982 r. Określano wpływ zmienności warunków słodowania: namoczenie jęczmienia do 43 i 46%, czas słodowania 7 i 9 dni, pH wody z dodatkiem kwasu giberelinowego (K.G.) od 5,5 do 8,5, dawki K.G. w zakresie 0,2, 0,3 i 0,5 mg/kg. W doświadczalnych jęczmieniach oznaczano: wilgotność, ekstraktywność, białko ogółem, skrobię, wagę 1000 ziaren, celność ziarna i energię kiełkowania. Analiza słodów obejmowała: wilgotność, ekstraktywność w mące i śrucie, białko ogółem, białko taninowe, barwę brzeczki, liczbę Kolbacha, garbniki ogółem, lepkość brzeczki, azot α-aminokwasowy, zawartość α-amylazy (metodą maltozową Briggsa), siłę diastatyczną według Windisch-Kolbacha, zawartość endo-β-glukanazy i zawartość endopeptydaz. Najwyższe zawartości α-amylazy (tab. 2) dawała odmiana Polon (570 j. FS), niższe Trumpf (o ok. 7%), najniższe odmiana paszowa Diva (o ok. 20%). Za korzystne warunki nagromadzania α-amylazy w słodzie bez dodatku kwasu giberelinowego (K.G.) uznano: namoczenie ziarna do wilgotności ok. 46%, temperaturę słodowania ok. 18°C i czas słodowania 7 dni (tab. 2 i 3). Przy ilościach dodawanego kwasu giberelinowego od 0,2 do 0,3 mg/kg jęczmienia i pH wody z K.G. od 6,5 do 7,5 średni przyrost zawartości α-amylazy dla badanych odmian wynosił 54% w porównaniu z próbami kontrolnymi bez K.G. (tab. 5). Dla odmiany Diva przyrost ten wynosił 57%. Opracowane warunki słodowania z dodatkiem K.G. stwarzają możliwości produkcji z jęczmienia odmiany Diva słodu piwowarskiego.
Źródło:
Acta Alimentaria Polonica; 1986, 12, 2; 91-101
0137-1495
Pojawia się w:
Acta Alimentaria Polonica
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Effect of thyroxin induction on enzymatic and biochemical levels in albino rats
Autorzy:
Hanchinalmath, J.V.
Shivasharanappa, K.
Roopa, T.S.
Saisundeep, Y.
Powiązania:
https://bibliotekanauki.pl/articles/11503.pdf
Data publikacji:
2014
Wydawca:
Przedsiębiorstwo Wydawnictw Naukowych Darwin / Scientific Publishing House DARWIN
Tematy:
thyroxine
alpha-amylase
enzymatic level
biochemical level
albino rat
rat
kidney
liver
blood serum
creatine phosphokinase activity
cholesterol
citric acid
triglyceride
Opis:
The albino Wistar rats were used to investigate the effect of thyroxine administration on the activity of citric acid, α-Amylase, creatine phosphokinase (CPK), creatinine, cholesterol and triglycerides level in blood serum and citric acid in liver and kidney. Two groups of rats were taken for the experiment. One group was injected subcutaneously with 200 μl of thyroxine/50 g of body weight, the other group served as control. The animals were vivisected with anesthesia after intervals of 24, 48, 72 and 96 hours α-Amylase and CPK levels in blood; citric acid level in liver and kidney of control and thyroxine treated rats. The results showed that the α-Amylase activity remained unaltered (100 g/h/l) throughout the experiment. However, citric acid level found to be 460 mg in the liver and 415 mg in kidney of control rats. The values slightly increased to 480 and 680 mg in respective tissues of thyroxine treated animals for 24 hr. The values decreased in 48 and 72 hours of treatment. Further the values found to be increased after 96 hours of treatment. Although the thyroxine treatment altered citric acid, Creatinine, Cholesterol, Triglycerides and CPK levels in the tissues, the serum α-Amylase was not influenced by the thyroxine. The study suggests that the thyroxine acts as a regulator all the above parameters in the cellular level.
Źródło:
International Letters of Natural Sciences; 2014, 08, 2
2300-9675
Pojawia się w:
International Letters of Natural Sciences
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Effectiveness of hemoperfusion in treatment of patients with non-biliary moderately severe pancreatitis
Skuteczność zastosowania hemoperfuzji w leczeniu pacjentów z ostrym martwiczym zapaleniem trzustki
Autorzy:
Oliynyk, O.
Ślifirczyk, A.
Pereviznyk, B.
Kushnarov, W.
Powiązania:
https://bibliotekanauki.pl/articles/2052946.pdf
Data publikacji:
2018
Wydawca:
Akademia Bialska Nauk Stosowanych im. Jana Pawła II w Białej Podlaskiej
Tematy:
acute pancreatitis
hemoperfusion
amylase
procalcitonin
ostre zapalenie trzustki
hemoperfuzja
amylaza
prokalcytonina
Opis:
Background. Acute pancreatitis morbidity has been rising annually all around the world. In case of acute necrotic pancreatitis, the mortality reaches 40% in the majority of hospitals. The therapy is effective if the efferent methods involve complex therapy of this disease. One such method is hemoperfusion, i.e. extracorporeal blood purification, which is widely used in toxicology. The authors of the article used hemoperfusion in a complex therapy in patients with severe acute pancreatitis. The following research presents an evaluation of the results of hemoperfusion used in a complex therapy of patients with acute pancreatitis. Material and methods. The study involved 38 patients with acute non-biliary moderately severe pancreatitis who were treated at an intensive care unit of Ternopil University Hospital in Ukraine. 18 patients were treated following the protocol for treatment of acute pancreatitis. In 20 patients, this treatment was additionally combined with hemoperfusion. We determined the levels of amylase, diastase, procalcitonin, bilirubin, malonic dialdehyde, and diene conjugates in blood serum and the level of leukocytes in the blood. Results. The levels of procalcitonin, amylase, bilirubin, leukocytes number, malonic dialdehyde, and diene conjugates were stabilised in patients of both groups, but in those who received hemoperfusion demonstrated much better results. These were manifested by significantly better levels of the amylase, bilirubin, creatinine, urea, procalcitonin, malonic dialdehyde, and diene conjugates in patients who received hemoperfusion (p< 0.005) than in the other group undergoing standard drug therapy. Conclusions. Hemoperfusion can be used as an effective method in the complex treatment of patients with acute pancreatitis.
Wprowadzenie. Corocznie na całym świecie obserwuje się w zrost zachorowalności na ostre zapalenie trzustki. W przypadku ostrego martwiczego zapalenia trzustki śmiertelność w większości szpitali osiąga 40%. Terapia jest skuteczna, jeśli metody odprowadzające obejmują kompleksową terapię tej choroby. Jedną z takich metod jest hemoperfuzja, tj. pozaustrojowa hemodializa krwi, szeroko stosowane w toksykologii. Autorzy artykułu zastosowali hemoperfuzję w kompleksowej terapii u pacjentów z ciężkim ostrym zapaleniem trzustki. Poniższe badania przedstawiają ocenę wyników hemoperfuzji stosowanej w kompleksowej terapii pacjentów z ostrym zapaleniem trzustki. Materiał i metody. W badaniu wzięło udział 38 pacjentów z ostrym, niezłośliwym i umiarkowanie ciężkim zapaleniem trzustki; leczonych w oddziale intensywnej opieki medycznej szpitala uniwersyteckiego na Ukrainie. Zgodnie z protokołem leczenia ostrego zapalenia trzustki leczono18 pacjentów. U 20 pacjentów leczenie to połączono dodatkowo z hemoperfuzją. Określono poziom amylazy, diastazy, prokalcytoniny, bilirubiny, dialdehydu malonowego i koniugatów dienowych w surowicy krwi oraz poziom leukocytów we krwi. Wyniki. Poziom prokalcytoniny, amylazy, bilirubiny, liczby leukocytów, dialdehydu malonowego i koniugatów dienowych były stabilizowane u pacjentów z obu grup, ale u tych pacjentów, u których włączono hemoperfuzję, wyniki były zdecydowanie lepsze. Przejawiały się one znacznie wyższym poziomem amylazy, bilirubiny, kreatyniny, mocznika, prokalcytoniny, dialdehydu malonowego i dienowych koniugatów u pacjentów, którym włączono hemoperfuzję hemoperfuzję (p <0,005) niż w drugiej grupie poddawanej standardowej terapii lekowej. Wnioski. Hemoperfuzja może być stosowana jako skuteczna metoda w kompleksowym leczeniu pacjentów z ostrym zapaleniem trzustki.
Źródło:
Health Problems of Civilization; 2018, 12, 3; 204-208
2353-6942
2354-0265
Pojawia się w:
Health Problems of Civilization
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Evaluation of salivary alpha amylase activity in smokers with periodontitis, Khartoum state, 2023
Autorzy:
Abd Alwhab Awad Allah, Fatima
Elrheima Ahmed, Hafsa Ahmed
Powiązania:
https://bibliotekanauki.pl/articles/29519597.pdf
Data publikacji:
2023-12-31
Wydawca:
Uniwersytet Rzeszowski. Wydawnictwo Uniwersytetu Rzeszowskiego
Tematy:
alpha-amylase
periodontitis
saliva
smokers
Opis:
Introduction and aim. Smoking widely affect oral health, including its role in the development of periodontitis. Saliva contains an antioxidant system and various enzymes. The study was designed to evaluate the activity of salivary alpha amylase among individuals who smoke and have periodontitis. Material and methods. A total of 100 participants were included, with 50 cases (cigarette smokers with periodontitis) and the remaining 50 nonsmokers with healthy periodontium as the control group. Saliva samples were collected to measure salivary alpha amylase activity. Results. Smokers with periodontitis had significantly higher levels of salivary alpha amylase compared to the control group(177.96±14.5 vs 94.04±19.6 IU/mL, p<0.001). Additionally, there was a weak negative correlation between the level of alpha amylase and the age of the patients (p=0.01, r=0.376). However, no correlation was found between the level of alpha amylase and the duration of smoking (p=0.584, r=0.079). Conclusion. There is a significant increase in salivary alpha amylase levels among smokers with periodontitis. No correlation was found between age and salivary alpha amylase levels. However, a weak positive correlation was observed between the duration of smoking and salivary alpha amylase activity.
Źródło:
European Journal of Clinical and Experimental Medicine; 2023, 4; 692-695
2544-2406
2544-1361
Pojawia się w:
European Journal of Clinical and Experimental Medicine
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Genetic polymorphism and quantitative variation of alpha-amylases from rye endosperm
Autorzy:
Masojc, P
Lapinski, M.
Larsson-Raznikiewicz, M.
Powiązania:
https://bibliotekanauki.pl/articles/2048192.pdf
Data publikacji:
1995
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
chromosome
rye
Secale cereale
colorimetric assay
isoenzyme
alpha-amylase
electrophoresis
endosperm
isoelectric focusing
immunoelectrophoresis
cereal cultivar
genetic polymorphism
Opis:
α-Amylase isozymes from rye endosperm were analysed by means of isoelectric focusing, polyacrylamide gel electrophoresis, immunoelectrophoresis and colorimetric assay. Α-AMY 1 (high pI) group was separated into 13 IEF bands, whereas in group α-AMY2 (low pI) 2 intensive and 6-8 faint bands were found. Two linked (2±1 cM) polymorphic loci and a single locus with two alleles encoding for α-AMY 1 and α-AMY 2 groups, respectively, were identified after genetic analysis of the IEF patterns. All α-amylase isozymes developed on PAGE, were shown to belong to α-AMY 1 group. It was demonstrated that a single PAGE isozyme corresponds to 2-4 separate IEF bands and that most of the IEF bands can be attributed to more than one PAGE isozyme. The activity of α-amylases from PAGE zone I was 2.3 times higher than the activity of zone II isozymes. A strong correlation between the activity and protein amount of particular α-AMY 1 isozymes (r=0.94) was found.
Źródło:
Journal of Applied Genetics; 1995, 36, 3; 197-204
1234-1983
Pojawia się w:
Journal of Applied Genetics
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Genetic regulation of alpha-amylase synthesis in rye [Secale cereale L.] grain
Autorzy:
Masojc, P
Stojalowski, S
Lapinski, M
Miazga, D
Powiązania:
https://bibliotekanauki.pl/articles/2047279.pdf
Data publikacji:
1996
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
enzyme synthesis
activity
chromosome
rye
Secale cereale
crossing
alpha-amylase
sprouting line
duplicate gene
addition line
genetic regulation
Opis:
Genetic analysis of two rye interline crosses and a set of wheat/rye chromosomal addition lines was performed to reveal the mechanism underlying wide variation range of alpha-amylase activity in sound grain. The long arm of chromosome 6R was found to be responsible for increased enzyme synthesis during late stages of triticale grain maturation. Only nuclear genes seemed to control alpha-amylase activity, as reciprocal crosses between rye lines showed no maternal effects. Low enzyme activity showed complete dominance over high level of its synthesis. Segregation ratios, observed in F₂ and BC₁ crosses, indicated that recessive alleles at two independent duplicative loci underlie intensive alpha-amylase production.
Źródło:
Journal of Applied Genetics; 1996, 37, 2; 141-152
1234-1983
Pojawia się w:
Journal of Applied Genetics
Dostawca treści:
Biblioteka Nauki
Artykuł
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