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Tytuł:
Purification and characterization of α-amylases from the intestine and muscle of Ascaris suum (Nematoda).
Autorzy:
Żółtowska, Krystyna
Powiązania:
https://bibliotekanauki.pl/articles/1044107.pdf
Data publikacji:
2001
Wydawca:
Polskie Towarzystwo Biochemiczne
Tematy:
Ascaris suum
α-amylase
starch digestion
glycogen metabolism
nematode
Opis:
α-Amylase (EC 3.2.1.1) was purified from the muscle and intestine of the parasitic helminth of pigs Ascaris suum. The enzymes from the two sources differed in their properties. Isoelectric focusing revealed one form of α-amylase from muscles with pI of 5.0, and two forms of amylase from intestine with pI of 4.7 and 4.5. SDS/PAGE suggested a molecular mass of 83 kDa and 73 kDa for isoenzymes of α-amylases from intestine and 59 kDa for the muscle enzyme. α-Amylase from intestine showed maximum activity at pH 7.4, and the enzyme from muscle at pH 8.2. The muscle enzyme was more thermostabile than the intestinal α-amylase. Both the muscle and intestine amylase lost half of its activity after 15 min at 70°C and 50°C, respectively. The Km values were: for muscle amylase 0.22 μg/ml glycogen and 3.33 μg/ml starch, and for intestine amylase 1.77 μg/ml glycogen and 0.48 μg/ml starch. Both amylases were activated by Ca2+ and inhibited by EDTA, iodoacetic acid, p-chloromercuribenzoate and the inhibitor of α-amylase from wheat. No significant differences were found between the properties of α-amylases from parasites and from their hosts.
Źródło:
Acta Biochimica Polonica; 2001, 48, 3; 763-774
0001-527X
Pojawia się w:
Acta Biochimica Polonica
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Content of glycogen and trehalose and activity of alpha-amylase and trehalase in Galleria mellonella larvae infected with entomopathogenic nematodes Steinernema affinis and S. feltiae
Autorzy:
Zoltowska, K
Lopienska-Biernat, E.
Powiązania:
https://bibliotekanauki.pl/articles/839414.pdf
Data publikacji:
2006
Wydawca:
Polskie Towarzystwo Parazytologiczne
Tematy:
Galleria mellonella
Steinernema feltiae
insect host
amylase
trehalase
host
parasite
trehalose
glycogen
parasitology
alpha-amylase
nematode
larva
Steinernema affinis
entomopathogenic nematode
Opis:
Introduction. The influence of infection with two species of entomopathogenic nematodes of Steinernematidae family on metabolism of glycogen and trehalose of the host was studied. Material and methods. Last instar larvae (L₇) of Galleria mellonella were experimentally infected with Steinernema affinis and S. feltiae. At 6, 12, 18 and 24 h after infection concentrations of trehalose and glycogen as well as activity of trehalase and α-amylase were determined. Results. The content of glycogen was lower in insects infected with S. feltiae than in the controls and animals infected with S. affinis. The content of trehalose was higher in insects from both infected groups than in the controls. Its concentration was slightly higher in larvae infected with S. affinis than in those infected with S. feltiae. The activity of α-amylase after infection with S. affinis was low. It was significantly higher in insects infected with S. feltiae. In animals of both infected groups, following a significant reduction at 6 h, the activity of trehalase remained at a similar level, higher than in the controls. In the paper the effects of infection with (i) different species of entomopathogenic nematodes and (ii) the importance of the developmental stage of the insect-host for changes in its metabolism of glycogen and trehalose were discussed.
Źródło:
Annals of Parasitology; 2006, 52, 2
0043-5163
Pojawia się w:
Annals of Parasitology
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Content of glycogen and trehalose and activity of alpha-amylase and trehalase in Galleria mellonella larvae infected with entomopathogenic nematodes Steinernema affinis and S. feltiae
Autorzy:
Żółtowska, K.
Łopieńska-Biernat, E.
Powiązania:
https://bibliotekanauki.pl/articles/2144330.pdf
Data publikacji:
2006
Wydawca:
Polskie Towarzystwo Parazytologiczne
Tematy:
Galleria mellonella
Steinernema feltiae
insect host
amylase
trehalase
host
parasite
trehalose
glycogen
parasitology
alpha-amylase
nematode
larva
Steinernema affinis
entomopathogenic nematode
Opis:
Introduction. The influence of infection with two species of entomopathogenic nematodes of Steinernematidae family on metabolism of glycogen and trehalose of the host was studied. Material and methods. Last instar larvae (L₇) of Galleria mellonella were experimentally infected with Steinernema affinis and S. feltiae. At 6, 12, 18 and 24 h after infection concentrations of trehalose and glycogen as well as activity of trehalase and α-amylase were determined. Results. The content of glycogen was lower in insects infected with S. feltiae than in the controls and animals infected with S. affinis. The content of trehalose was higher in insects from both infected groups than in the controls. Its concentration was slightly higher in larvae infected with S. affinis than in those infected with S. feltiae. The activity of α-amylase after infection with S. affinis was low. It was significantly higher in insects infected with S. feltiae. In animals of both infected groups, following a significant reduction at 6 h, the activity of trehalase remained at a similar level, higher than in the controls. In the paper the effects of infection with (i) different species of entomopathogenic nematodes and (ii) the importance of the developmental stage of the insect-host for changes in its metabolism of glycogen and trehalose were discussed.
Źródło:
Wiadomości Parazytologiczne; 2006, 52, 2; 103-107
0043-5163
Pojawia się w:
Wiadomości Parazytologiczne
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Bioethanol Production from Chlorella Pyrenoidosa by Using Enzymatic Hydrolysis and Fermentation Method
Autorzy:
Yerizam, Muhammad
Jannah, Asyeni Miftahul
Aprianti, Nabila
Powiązania:
https://bibliotekanauki.pl/articles/2202327.pdf
Data publikacji:
2023
Wydawca:
Polskie Towarzystwo Inżynierii Ekologicznej
Tematy:
Chlorella pyrenoidosa
hydrolysis
enzyme
alpha-amylase
glucose
fermentation
bioethanol
Opis:
Starch can be found in microalgae, the raw material for the third generation of bioethanol production. One of them is C. pyrenoidosa. This study was conducted to analyze the effect of α-amylase enzyme concentration on the glucose contents produced and the effects of fermentation time on bioethanol contents produced. The hydrolysis process of this study was conducted using an α-amylase enzyme produced by A. niger. Several analyses in this research were carried out, including the analysis of enzyme activity using the Fuwa method, the analysis of glucose levels from enzymatic hydrolysis using the DNS method, and the analysis of bioethanol contents using the density method and GC-MS. The highest glucose content was 0.67 mg/mL, which was obtained from the addition of 40% (v/w) α-amylase enzyme, and the yield of bioethanol content from the sample treated 40% (v/w) α-amylase enzyme and fermented for 9 days was the optimum, which produced 28.07% of bioethanol content.
Źródło:
Journal of Ecological Engineering; 2023, 24, 1; 34--40
2299-8993
Pojawia się w:
Journal of Ecological Engineering
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
DIPSACUS FULLONUM L. LEAVES AND ROOTS - IDENTIFICATION OF THE COMPONENTS OF THE ESSENTIAL OILS AND ALPHA-AMYLASE INHIBITORY ACTIVITIES OF METHANOLIC EXTRACTS
Autorzy:
Witkowska-Banaszczak, Ewa
Powiązania:
https://bibliotekanauki.pl/articles/895320.pdf
Data publikacji:
2018-08-31
Wydawca:
Polskie Towarzystwo Farmaceutyczne
Tematy:
essential oil composition
methanolic extract
alpha-amylase inhibition
Opis:
Abstract: Dipsacus fullonum L. syn. D. sylvestris is a biennial plant from the Dipsacaceae family. It grows in Europe, North Africa and Asia Minor. It has been used in folk medicine mainly to treat Lyme disease. This study was undertaken to identify and compare the components of the essential oils from dried and fresh roots, which are usually applied as medicinal agents, as well as those of the essential oils from dried and fresh leaves, used less frequently. The essential oils from the Dipsacus fullonum leaves and roots were analysed by the GC and GC-MS analysis. 18 components were identified in the essential oil from the fresh leaves, whereas 37 were recognised in the oil from the dried leaves; 10 components were identified in the essential oil from the fresh roots and 40 in that from the dried roots. Phytol was the main component in the essential oil from the leaves, whereas n-hexadecanoic acid was predominant in the roots. The α -amylase inhibition has been proven for methanolic extracts of D. fullonum leaves and roots. The strongest activity was demonstrated by the extract from fresh leaves of D. fullonum and showed an effect about one hundred times weaker than acarbose.
Źródło:
Acta Poloniae Pharmaceutica - Drug Research; 2018, 75, 4; 951-957
0001-6837
2353-5288
Pojawia się w:
Acta Poloniae Pharmaceutica - Drug Research
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
The influence of varying level of wheat proteinaceous inhibitors in wheat-based artificial feed on development parameters of selected stored product insects
Autorzy:
Warchalewski, J R
Gralik, J.
Winiecki, Z.
Nawrot, J.
Piasecka-Kwiatkowska, D.
Powiązania:
https://bibliotekanauki.pl/articles/65768.pdf
Data publikacji:
2001
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
wheat starch
varying level
weevil
plant protection
wheat grain
flour moth
Sitophilus granarius
insect
Tribolium confusum
feed
insect foraging
development parameter
Anagasta kuehniella
feeding intensity
alpha-amylase inhibitor
artificial wheat-based feed
wheat
larval development
flour beetle
artificial feed
granary weevil
Opis:
The artificially made kernels from ground wheat grain, commercial wheat starch and wheat proteinaceous α-amylase inhibitors in different proportions were used as feed for adults of the granary weevil (Sitophilus granarius L.). In the case of larvae of the confused flour beetle (Tribolium confusum Duv.) and the Mediterranean flour moth (Anagasta kuehniella Zell.) the friable feed mixture were used. The survival of S. granarius adults has not been correlated with the soluble proteins extracted from wheat and amylolytic activity located in this protein fraction. On the other hand the weight of dust (the index of feeding intensity) produced during feeding has depended on the presence of α-amylase and trypsin inhibitors in wheat-based feed. A. kuehniella larvae have not developed at all on feed consisted of 50% wheat starch and 50% of crude a-amylase inhibitors from wheat. The same feed has caused 15.1 days of extension in development time of T. confusum larvae. lt attests to specific native enzymatic apparatus existing in alimentary canals of three damaging grain species which can overcome some obstacles even if extremely highly active insect α-amylase inhibitors were present in feed. However, the sufficient nutrient should be available in feed compounds. Nevertheless, some reduction of insects population can be expected.
Sztuczne ziarna zrobione z handlowej skrobii pszennej i białkowych inhibitorów α-amylaz wymieszanych w różnych proporcjach oraz czyste ziarno pszenicy stanowiły pokarm wołka zbożowego (Sitophilus granaris L.). Dla larw trojszyka ulca (Trbolium confusum Duv.) i mklika mącznego (Anagasta kuchniella Zell.) te same produkty podawano w postaci sypkiej. Procent przeżywalności chrząszczy wołka zbożowego nie zależał od zawartości białka rozpuszczalnego ani od aktywności amylolitycznej pokarmu. Z drugiej strony ilość pyłu wytworzonego w czasie żerowania (wskaźnik intensywności żerowania) zależał od obecności inhibitorów α-amylazy i trypsyny. Larwy mklika mącznego nie kończyły rozwoju na pokarmie zawierającym 50% skrobii pszennej i 50 inhibitorów α-amylazy. Ten sam pokarm powodował przedłużenie rozwoju larw trojszyka ulca o 15,1 dnia. To świadczy o istnieniu wrodzonego, specyficznego aparatu enzymatycznego w przewodzie pokarmowym testowanych owadów, który może pokonać nawet ekstremalnie wysoką aktywność inhibitorów α-amylazy obecnych w pokarmie. Efektem działania inhibitorów może być tylko redukcja liczebności populacji.
Źródło:
Journal of Plant Protection Research; 2001, 41, 3
1427-4345
Pojawia się w:
Journal of Plant Protection Research
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
The relationship of biochemical parameters and radiological parameters in the evaluation of the clinical severity of acute pancreatitis in the emergency department – a retrospective analysis
Autorzy:
Tortum, Fatma
Tekin, Erdal
Aydın, Fahri
Özdal, Emine
Tatlısu, Kübra
Powiązania:
https://bibliotekanauki.pl/articles/25102374.pdf
Data publikacji:
2023-06-30
Wydawca:
Uniwersytet Rzeszowski. Wydawnictwo Uniwersytetu Rzeszowskiego
Tematy:
acute pancreatitis
amylase
computed tomography severity index
emergency department
lipase
Opis:
Introduction and aim. Computed tomography severity index (CTSI) and Balthazar score are among the most frequently used scorings in the determination of severe acute pancreatitis. The primary purpose of this study is evaluation of the effects of biochemical parameters, Balthazar score and CTSI on mortality in acute pancreatitis. At the same time, correlations with biochemical parameters, CTSI and Balthazar score were evaluated in patients with AP. Material and methods. In this study, the amylase, lipase, CRP, and procalcitonin values of patients diagnosed with acute pancreatitis were retrospectively recorded. Contrast-enhanced computed tomography (CECT) images obtained at the time of presentation to the emergency department or within seven days of admission were re-evaluated by two radiologists. The CTSI scores and Balthazar scores of the patients were calculated. Results. The study included 240 patients. The amylase level of the patients was positively correlated with the Balthazar score at a statistically significant level (R=0.189, p=0.003). In addition, the relationship between pancreatic scoring systems and mortality, the AUC value for CTSI was 0.9 (95% CI: 0.826-0.973) and was higher than other scoring systems. Conclusion. CTSI had better performance in the prediction of mortality in patients with acute pancreatitis.
Źródło:
European Journal of Clinical and Experimental Medicine; 2023, 2; 277-282
2544-2406
2544-1361
Pojawia się w:
European Journal of Clinical and Experimental Medicine
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Screening and characterization of thermo-active enzymes of biotechnological interest produced by thermophilic Bacillus isolated from hot springs in Tunisia
Autorzy:
Thebti, Wajdi
Riahi, Yosra
Gharsalli, Rawand
Belhadj, Omrane
Powiązania:
https://bibliotekanauki.pl/articles/1038787.pdf
Data publikacji:
2016
Wydawca:
Polskie Towarzystwo Biochemiczne
Tematy:
temperature
amylase
protease
cellulase
xylanase
mannanase
Geobacillus
Tunisian hot springs
Opis:
As part of the contribution to the global efforts in research of thermostable enzymes being of industrial interest, we focus on the isolation of thermophilic bacteria from Tunisian hot springs. Among the collection of 161 strains of thermophilic Bacillus isolated from different samples of thermal water in Tunisia, 20% are capable of growing at 100°C and the rest grow at 70°C or above. Preliminary activity tests on media supplemented with enzyme-substrates confirmed that 35 strains produced amylases, 37 - proteases, 43 - cellulases, 31 - xylanases and 37 - mannanases. The study of the effect of temperature on enzyme activity led to determination of the optimal temperatures of activities that vary between 60 and 100°C. Several enzymes were active at high temperatures (80, 90 and 100°C) and kept their activity even at 110°C. Several isolated strains producing enzymes with high optimal temperatures of activity were described for the first time in this study. Both strains B62 and B120 are producers of amylase, protease, cellulase, xylanase, and mannanase. The sequencing of 16S DNA identified isolated strains as Geobacillus kaustophillus, Aeribacillus pallidus, Geobacillus galactosidasus and Geobacillus toebii.
Źródło:
Acta Biochimica Polonica; 2016, 63, 3; 581-587
0001-527X
Pojawia się w:
Acta Biochimica Polonica
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Influence of post-harvest maturation on the amylolytic activity of wheat grain and the properties of bread
Autorzy:
Szafrańska, Anna
Stępniewska, Sylwia
Powiązania:
https://bibliotekanauki.pl/articles/2148191.pdf
Data publikacji:
2021-12-30
Wydawca:
Instytut Uprawy Nawożenia i Gleboznawstwa – Państwowy Instytut Badawczy
Tematy:
alpha-amylase
bread
mixolab
technological value
wheat
Opis:
The aim of this study was to determine the quantitative and qualitative changes in the starch complex and the amylolytic activity of wheat grain during its post-harvest maturity. This in- formation is necessary for the proper determination of the level of quality requirements for wheat grain purchased immediately after harvest. The common wheat cultivars: Kandela, Bamberka and KWS Ozon were tested during this study. Because of the variability of weather conditions, three wheat cultivars were tested (two winter wheat cultivars and one spring wheat cultivar) from two consecu- tive crop years. Immediately after grain harvest and during the next 12 weeks in two-weeks intervals the falling number, amylo- graph properties, rheological properties of dough and laboratory baking test was performed. Tested wheat cultivars were characterized by low amylolytic activity. Changes of the baking value of tested wheat cultivars during their post-harvest maturation were dependent on wheat cultivar and harvest year. Wheat from the 2016 harvest was char- acterized by significantly lower amylolytic activity compared to grain from 2015 harvest. Amylolytic activity decreased during post-harvest maturation, i.e. the falling number increased as well as the dough torque in point C4 and C5 of mixolab curve. There was also an increase in the volume of bread compared to the evaluation of the samples tested immediately after harvest. The highest values were obtained in the 4th week after harvest. The changes were dependent on wheat cultivar. However, in the case of the assessment of the rheological characteristics of the dough (except point C4) they were not statistically significant.
Źródło:
Polish Journal of Agronomy; 2021, 47; 58-67
2081-2787
Pojawia się w:
Polish Journal of Agronomy
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Zmiany właściwości wypiekowych mąki żytniej pod wpływem dodatku alfa-amylazy
Changes in baking properties of rye flour impacted by alpha-amylase addition
Autorzy:
Szafranska, A.
Slowik, E.
Powiązania:
https://bibliotekanauki.pl/articles/35734.pdf
Data publikacji:
2014
Wydawca:
Polska Akademia Nauk. Instytut Agrofizyki PAN
Tematy:
piekarstwo
maka zytnia
wartosc wypiekowa
dodatki do zywnosci
alfa-amylazy
ciasto
chleb
pieczywo
jakosc
baking
rye flour
baking value
food additive
alpha-amylase
dough
bread
quality
Opis:
Celem podjętych badań było określenie wpływu dodatku alfa-amylazy do mąki żytniej na właściwości ciasta i jakość pieczywa. Materiał badawczy stanowiły trzy próbki mąki żytniej typu 720 o niskiej i bardzo niskiej aktywności enzymów amylolitycznych oraz alfaamylaza, którą dodawano w ilości 15, 20 i 40 g·(100 kg mąki)-1. Aktywność amylolityczną próbek mąki wyjściowej i z dodatkiem alfaamylazy oceniano na podstawie badań amylograficznych i liczby opadania. Przeprowadzono badania reologiczne ciasta za pomocą mixolabu. Wykonano wypieki laboratoryjne. Stwierdzono, że dodatek alfa-amylazy do mąki żytniej powodował obniżenie wysokich wartości parametrów amylograficznych mąki: maksymalnej lepkości i temperatury końcowej kleikowania. Ciasto z mąki żytniej o większym dodatku alfa-amylazy cechowało się mniejszym oporem w punktach wykresu uzyskanego za pomocą mixolabu charakteryzującym właściwości skrobi: kleikowanie (opór ciasta w punkcie C3), podatność na działanie enzymów amylolitycznych (opór ciasta w punkcie C4) i retrogradację (opór ciasta w punkcie C5). Stwierdzono, że wraz ze zmniejszeniem oporu ciasta w punkcie C2, w którym rozpoczyna się kleikowanie skrobi i w punkcie C3 wykresu oraz tempa kleikowania skrobi (wskaźnik β) zwiększała się objętość chleba (współczynniki korelacji odpowiednio r = –0,670; r = –0,726 i r = –0,651). Jakość chleba pod wpływem dodatku alfa-amylazy ulegała poprawie – wzrastała objętość chleba i kwasowość miękiszu a zmniejszała się jego twardość.
The aim of this study was to determine the effect of alpha-amylase addition on the properties of dough and on the quality of rye bread. The experimental material was three samples of rye flour type 720 with low and very low amylolytic activity. Alpha-amylase was added in the amounts of 15, 20 and 40 g (100 kg flour)-1. Amylolytic activity of tested rye flour without and with alpha-amylase addition was assessed by the amylograph properties and falling number method. The rheological properties of dough were tested by mixolab. The laboratory test baking was performed. Alpha-amylase addition to rye flour reduced high values of amylograph properties: maximum vis-cosity and final temperature of gelatinisation. Rye dough obtained from flour with higher alpha-amylase addition was characterised by lower mixolab torque in points which characterise starch gelatinisation (torque in point C3), enzymatic activity (torque in point C4) and retrogradation (torque in point C5). The rye dough with lower torque in point C2 in which gelatinization starts and in point C3 and lower slope β was characterised by higher bread volume (correlation coefficients: r = –0,670; r = –0,726; r = –0,651 respectively). Better quality of bread obtained from rye flour with alpha-amylase addition was observed – the bread volume and titrable acidity increased, and crumb hardness decreased.
Źródło:
Acta Agrophysica; 2014, 21, 2
1234-4125
Pojawia się w:
Acta Agrophysica
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Effect of steeping degree, malting temperature and addition of gibberellic acid on the accumulation of alpha-amylase in malt
Wpływ stopnia namoczenia, temperatury słodowania i dodatku kwasu giberelinowego na nagromadzenie alfa-amylazy w słodzie
Autorzy:
Surmiński, J.
Masior, S.
Kuchciak, T.
Powiązania:
https://bibliotekanauki.pl/articles/1398778.pdf
Data publikacji:
1986
Wydawca:
Instytut Rozrodu Zwierząt i Badań Żywności Polskiej Akademii Nauk w Olsztynie
Tematy:
malting harley
a-amylase
giberellic acid
Opis:
Malting on a microtechnological scale was performed with the use of brewer's barley of Trumpf and Polon varieties and with the feed variety Diva, all from the 1982 crop. The highest α-amylase content was found in the Polon variety; this content in the Trumpf variety was lower by about 7%, while in the feed variety Diva it was lower by about 20%. The following conditions were found to favour the accumulation of α-amylase in malt containing no addition of gibberellic acid (g.a.): malted grain humidity - 46%, malting temperature - 18°C, time of malting - 7 days. When gibberellic acid was added in doses of 0.2-0.3 mg/kg barley and when pH of the water with g.a. ranged from 6.5 to 7.5, the mean increase of α-amylase content in the studied malt varieties was 54% as compared to control samples without g.a. In the case of the variety Diva this increase was 57%. The conditions of malting with an addition of g.a. that were worked out allow the production of brewer's malt from Diva barley.
Do słodowania w skali mikrotechnicznej użyto krajowe odmiany browarne jęczmienia: Trumpf i Polon oraz odmianę paszową Diva ze zbioru 1982 r. Określano wpływ zmienności warunków słodowania: namoczenie jęczmienia do 43 i 46%, czas słodowania 7 i 9 dni, pH wody z dodatkiem kwasu giberelinowego (K.G.) od 5,5 do 8,5, dawki K.G. w zakresie 0,2, 0,3 i 0,5 mg/kg. W doświadczalnych jęczmieniach oznaczano: wilgotność, ekstraktywność, białko ogółem, skrobię, wagę 1000 ziaren, celność ziarna i energię kiełkowania. Analiza słodów obejmowała: wilgotność, ekstraktywność w mące i śrucie, białko ogółem, białko taninowe, barwę brzeczki, liczbę Kolbacha, garbniki ogółem, lepkość brzeczki, azot α-aminokwasowy, zawartość α-amylazy (metodą maltozową Briggsa), siłę diastatyczną według Windisch-Kolbacha, zawartość endo-β-glukanazy i zawartość endopeptydaz. Najwyższe zawartości α-amylazy (tab. 2) dawała odmiana Polon (570 j. FS), niższe Trumpf (o ok. 7%), najniższe odmiana paszowa Diva (o ok. 20%). Za korzystne warunki nagromadzania α-amylazy w słodzie bez dodatku kwasu giberelinowego (K.G.) uznano: namoczenie ziarna do wilgotności ok. 46%, temperaturę słodowania ok. 18°C i czas słodowania 7 dni (tab. 2 i 3). Przy ilościach dodawanego kwasu giberelinowego od 0,2 do 0,3 mg/kg jęczmienia i pH wody z K.G. od 6,5 do 7,5 średni przyrost zawartości α-amylazy dla badanych odmian wynosił 54% w porównaniu z próbami kontrolnymi bez K.G. (tab. 5). Dla odmiany Diva przyrost ten wynosił 57%. Opracowane warunki słodowania z dodatkiem K.G. stwarzają możliwości produkcji z jęczmienia odmiany Diva słodu piwowarskiego.
Źródło:
Acta Alimentaria Polonica; 1986, 12, 2; 91-101
0137-1495
Pojawia się w:
Acta Alimentaria Polonica
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
In vitro α-glucosidase and α-amylase enzyme inhibitory effects of Andrographis paniculata extract and andrographolide
Autorzy:
Subramanian, Rammohan
Asmawi, M
Sadikun, Amirin
Powiązania:
https://bibliotekanauki.pl/articles/1040761.pdf
Data publikacji:
2008
Wydawca:
Polskie Towarzystwo Biochemiczne
Tematy:
α-glucosidase
α-amylase
Andrographis paniculata
andrographolide
peak blood glucose
post prandial hyperglycaemia.
Opis:
There has been an enormous interest in the development of alternative medicines for type 2 diabetes, specifically screening for phytochemicals with the ability to delay or prevent glucose absorption. The goal of the present study was to provide in vitro evidence for potential inhibition of α-glucosidase and α-amylase enzymes, followed by a confirmatory in vivo study on rats to generate a stronger biochemical rationale for further studies on the ethanolic extract of Andrographis paniculata and andrographolide. The extract showed appreciable α-glucosidase inhibitory effect in a concentration-dependent manner (IC50=17.2±0.15 mg/ml) and a weak α-amylase inhibitory activity (IC50=50.9±0.17 mg/ml). Andrographolide demonstrated a similar (IC50=11.0±0.28 mg/ml) α-glucosidase and α-amylase inhibitory activity (IC50=11.3±0.29 mg/ml). The positive in vitro enzyme inhibition tests paved way for confirmatory in vivo studies. The in vivo studies demonstrated that A. paniculata extract significantly (P<0.05) reduced peak blood glucose and area under curve in diabetic rats when challenged with oral administration of starch and sucrose. Further, andrographolide also caused a significant (P<0.05) reduction in peak blood glucose and area under the curve in diabetic rats. Hence α-glucosidase inhibition may possibly be one of the mechanisms for the A. paniculata extract to exert antidiabetic activity and indicates that AP extract can be considered as a potential candidate for the management of type 2 diabetes mellitus.
Źródło:
Acta Biochimica Polonica; 2008, 55, 2; 391-398
0001-527X
Pojawia się w:
Acta Biochimica Polonica
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Assessment of chemical composition of flour and its effect on baking characteristics of wheat
Autorzy:
Subda, H
Powiązania:
https://bibliotekanauki.pl/articles/1371393.pdf
Data publikacji:
1992
Wydawca:
Instytut Rozrodu Zwierząt i Badań Żywności Polskiej Akademii Nauk w Olsztynie
Tematy:
flour
wheat flour
glutenin
food
gliadin
chemical composition
high-molecular glutenin
proteolytic enzyme
wheat
alpha-amylase
baking
protein
total protein
Źródło:
Polish Journal of Food and Nutrition Sciences; 1992, 01, 2; 15-21
1230-0322
2083-6007
Pojawia się w:
Polish Journal of Food and Nutrition Sciences
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Wheat seedlings as affected by Mg(NO3)2 and ZnSO4 priming treatments
Autorzy:
Singhal, Rajesh Kumar
Bose, Bandana
Powiązania:
https://bibliotekanauki.pl/articles/1030251.pdf
Data publikacji:
2020
Wydawca:
Przedsiębiorstwo Wydawnictw Naukowych Darwin / Scientific Publishing House DARWIN
Tematy:
Mean germination time
Seed priming
Seedling vigour
Soluble and insoluble sugar content
Triticum aestivum
α-amylase activity
Opis:
Seed is the most crucial and prime input in agriculture, and the seed must be of high quality. Seed priming is one of the reliable and cost-effective strategies to enhance seed germination and seedling establishment in crops. This study was conducted to determine the effects of seed priming with Mg(NO3)2 (S1) and ZnSO4 (S2) in two wheat (Triticum aestivum L.) varieties (HUW-468 and HUW-510) on germination and seedling growth. Varied levels of Mg(NO3)2 and ZnSO4 from C0 to C11 were used for the present study and number of seed germinated, seedling establishment and biochemical parameters were recorded. From the study of germination %, mean germination time (MGT), mean germination rate (MGR), coefficient of velocity of germination (CVG), germination rate index (GRI) (% / day), germination index (GI), seedling vigor (SV), shoot, root and seedling length, α-amylase activity, soluble and insoluble sugar content and protein content, it was revealed that 7.5 mM of Mg(NO3)2 and 0.05 mM of ZnSO4 were the best treatments in improving seedling establishment, performance and biochemical status of wheat seed. Further, this analysis also reveals a strong correlation between the seedling establishment and the activity of -amylase and protein content.
Źródło:
World Scientific News; 2020, 144; 13-29
2392-2192
Pojawia się w:
World Scientific News
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Isolation and preliminary sequence characterization of beta-amylase gene promoters in rye [Secale cereale L.]
Autorzy:
Sadowski, J
Rorat, T
Cooke, R
Delseny, M
Powiązania:
https://bibliotekanauki.pl/articles/2046684.pdf
Data publikacji:
1997
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
beta-amylase
rye
gene promoter
homology
amino acid
DNA cloning
prolamin
Secale cereale
mRNA
embryogenesis
endosperm
cDNA
inverse polymerase chain reaction
Opis:
The isolation of rye ß-Amy1 and ß-Amy2 gene promoters from nuclear DNA using the inverse polymerase chain reaction (IPCR) technique and characterization of their sequences are presented. The conservation of ß-amylasc coding sequences allowed for simultaneous IPCR amplification of two different promoters with primers designed on the basis of the single known cDNA sequence. Two ß-amylasc gene promoters display a low sequence similarity (47%). Beside consensus TATA and CCAAT boxes, other sequence motives common to both promoters were found. In addition, the homology of amino acid sequences of plant ß-amylases available in the database is discussed.
Źródło:
Journal of Applied Genetics; 1997, 38, 3; 241-251
1234-1983
Pojawia się w:
Journal of Applied Genetics
Dostawca treści:
Biblioteka Nauki
Artykuł
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