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    Wyświetlanie 1-8 z 8
    Tytuł:
    EVALUATION OF MUTAGENIC PROPERTIES OF 2(C6H15N2O2)+2I3-H2O IODINE COORDINATION COMPLEX IN BACTERIAL REVERSE MUTATION TEST
    Autorzy:
    Jumagaziyeva, Ardak B.
    Iskakbayeva, Zhanar A.
    Myrzabayeva, Auyes N.
    Suldina, Natalya A.
    Paretskaya, Nataliya A.
    Datkhaev, Ubaidulla M.
    Flisyuk, Elena V.
    Ilin, Aleksandr I.
    Powiązania:
    https://bibliotekanauki.pl/articles/895360.pdf
    Data publikacji:
    2020-06-29
    Wydawca:
    Polskie Towarzystwo Farmaceutyczne
    Tematy:
    mutagenicity
    Ames test
    iodine coordination complex
    Opis:
    Objective: 2(C6H15N2O2)+ 2I3-H2O iodine coordination complex is a new pharmaceutical substance that have high antibacterial activity against both sensitive and multidrug resistant strains of Escherichia coli, Staphylococcus aureus, Pseudomonas aeruginosa. Methods: Mutagenicity of 2(C6H15N2O2)+2I3-H2O iodine coordination complex was assessed using Salmonella/microsome (Ames) test. Tester strains used were Salmonella typhimurium TA98, TA100, TA1535, TA 1537 and Escherichia coli WP2uvrA. The mutagenic activity was determined both in the absence or presence of S9 mixture. Results: The study have shown that test item did not cause any increase in the number of his+ revertants in S.typhimurium and trp+revertants in E.coli WP2uvrA strains in the presence or absence of S9-mix, compared to the controls. Conclusion: Under the conditions of this test and according to the criteria set for the evaluation of the test results, the 2(C6H15N2O2)+ 2I3-H2O iodine coordination complex did not show mutagenic activity in the S. typhimurium and E.coli plate incorporation assay.
    Źródło:
    Acta Poloniae Pharmaceutica - Drug Research; 2020, 77, 3; 465-473
    0001-6837
    2353-5288
    Pojawia się w:
    Acta Poloniae Pharmaceutica - Drug Research
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Computational predictive mutagenicity of similar chemicals for anthraquinone, β-sitosterol and quercetin found in Alternanthera tenella by using QSAR modeling software
    Autorzy:
    Haque, Mahmudul
    Bhakat, Ram Kumar
    Bhattacharjee, Aloke
    Talapatra, Soumendra Nath
    Powiązania:
    https://bibliotekanauki.pl/articles/1182926.pdf
    Data publikacji:
    2016
    Wydawca:
    Przedsiębiorstwo Wydawnictw Naukowych Darwin / Scientific Publishing House DARWIN
    Tematy:
    Allelopathy; Allelochemicals; Alternanthera tenella; Invasive species; Terrestrial ecosystem; Predictive Ames mutagenicity; QSAR modeling; T.E.S.T. software
    Opis:
    The present study aims to evaluate the mutagenic potential of secondary metabolites viz. anthraquinone, β-sitosterol and quercetin present in Alternanthera tenella and their related analogus compounds similar in their molecular structure. Nine similar putative allelochemicals analogus to each of anthraquninone, β-sitosterol and quercetin respectively were selected, a total of twenty seven similar chemicals were studied for mutagenicity prediction. Ames mutagenicity prediction was carried out by using T.E.S.T. (Toxicity Estimation Software Tool) of USEPA. All experimental metadata were obtained from Toxicity Benchmark and T.E.S.T. The results clearly indicated that the allelochemicals, anthraquinone and its related eight compounds were mutagenic positive except benzanthrone (mutagenic negative) but all experimental data were found mutagenic positive. β-sitosterol showed mutagenic negative in both experimental and predicted value. It’s three related compounds were mutagenic positive but rest six related compounds mutagenic negative in predicted value while in experimental data, seven compounds were found mutagenic positive and rest two mutagenic negative. In case of quercetin, both data were obtained mutagenic positive while in related compounds, seven compounds were found to be mutagenic positive and two compounds mutagenic negative in predicted value. All were found to be mutagenic positive in experimental metadata. Such findings poses a curiosity that are there any possibilities of conversion or substitution in the position of aromatic ring of allelochemicals when present in soil? Because allelopathy depends upon several environmental stressors and mutagenicity may be induced by allelochemicals. It is suggesting for future research to detect metabolic pathway and mechanism of allelochemicals formation in A. tenella in presence of toxins in soil and to validate with other available 2D and 3D softwares.
    Źródło:
    World Scientific News; 2016, 49, 2; 162-191
    2392-2192
    Pojawia się w:
    World Scientific News
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Assessment of mutagenic activity of methyl- and phenylphenanthrenes based on Salmonella test and micronucleus test
    Autorzy:
    Rudnicka, K.
    Tejs, S.
    Budzikur, K. A.
    Mielżyńska-Švach, D.
    Jakimiuk, E.
    Chachaj, A.
    Góra, M.
    Żelazna, K.
    Łuczyński, M. K.
    Powiązania:
    https://bibliotekanauki.pl/articles/363178.pdf
    Data publikacji:
    2013
    Wydawca:
    Uniwersytet Warmińsko-Mazurski w Olsztynie
    Tematy:
    Ames test
    in vitro
    micronucleus test
    mutagenicity
    phenanthrene
    test Amesa
    mutagenność
    test mikrojądrowy
    fenanatren
    Opis:
    Polycyclic aromatic hydrocarbons (PAHs) are widely spread environmental pollutants mainly originating from anthropogenic sources such as fossil fuel combustion, industries, and others. Although a large body of literature exists on the toxicity and carcinogenicity of PAHs, primarily benzo[a]pyrene, toxicity data for phenanthrene deriveratives are very limited. The main aim of the experiment was to investigate if there exists correlation between molecular structure and mutagenic activity of four phenanthrene derivatives: 1 methylphenanthrene, 4 methylphenanthrene, 1 phenylphenanthrene, and 4 phenylphenanthrene. An Ames assay using two strains of histidine dependent Salmonella Typhimurium (TA98 and TA100) was conducted to assess the mutagenic activity of studied compounds both in the presence (+S9) and in the absence (-S9) of an exogenous source of metabolic activation. The compounds were also tested in an in vitro chromosome aberration assay in which V-79 cells were exposed to the phenanthrene derivatives investigated both in the presence and in the absence of metabolic activation. The phenylphenanthrenes showed no mutagenic effect. These compounds occasionally induced significant decrease in the number of revertants in the Ames test. The greatest mutagenic effects were observed for 1 methylphenanthrene after metabolic activation (+S9). In the micronucleus test the greatest mutagenic effect was observed for 4 methylphenanthrene also in the presence of metabolic activation system. The results obtained are comparable to those reported earlier for the methylphenanthrenes.
    Źródło:
    Environmental Biotechnology; 2013, 9, 2; 65-71
    1734-4964
    Pojawia się w:
    Environmental Biotechnology
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    2,4-diphenylthiophene induces mainly base pair mutation in Salmonella Typhimurium
    Autorzy:
    Budzikur, K. A.
    Mielżyńska-Švach, D.
    Góra, M.
    Chachaj, A.
    Pawłowski, M.
    Łuczyński, M. K.
    Powiązania:
    https://bibliotekanauki.pl/articles/363086.pdf
    Data publikacji:
    2012
    Wydawca:
    Uniwersytet Warmińsko-Mazurski w Olsztynie
    Tematy:
    test Amesa
    związki heterocykliczne
    mutagenność
    siarkowe wielopierścieniowe węglowodory aromatyczne
    Ames test
    heterocyclic compounds
    mutagenicity
    sulfur-PAHs
    Opis:
    Heterocyclic aromatic compounds containing sulfur (S-HET), have been detected in air, soil, marine environment and freshwater sediment. Toxicity and mutagenicity data of this class of substances are scarce. The present study focuses on implications of two aryl thiophenes and their mutagenic properties in Salmonella/microsome test. In our experiment only 2,4-diphenylthiophene showed little mutagenic effect in both variants of activaction (+/-S9) in strain TA100. Thiophene ring joined to K-region of phenanthrene did not change the biological activity of 3,6-dimetoxyphenanthro [9,10-c]thiophene and this compound did not show mutagenic potency.
    Źródło:
    Environmental Biotechnology; 2012, 8, 1; 28-31
    1734-4964
    Pojawia się w:
    Environmental Biotechnology
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Mutagenicity induced in Salmonella strains TA98 and TA100 by diphenylthiophenes
    Autorzy:
    Budzikur, K. A.
    Góra, M.
    Chachaj, A.
    Mielżyńska-Švach, D.
    Tejs, S.
    Łuczyński, M. K.
    Powiązania:
    https://bibliotekanauki.pl/articles/363118.pdf
    Data publikacji:
    2011
    Wydawca:
    Uniwersytet Warmińsko-Mazurski w Olsztynie
    Tematy:
    test Amesa
    mutacja
    monomery tiofenowe
    Ames test
    mutation
    thiophene monomers
    Opis:
    Mutagenic properties of four different diphenylthiophenes: 3,4-diphenylthiophene, 3,4-di(4'-methylphenyl)thiophene, 3,4-di(4'-methoxyphenyl)thiophene and 3,4-di(4'-pentoxyphenyl)thiophene were investigated applying the Salmonella test. The research was done on two strains of Salmonella Typhimurium: TA98 and TA100, tested in two variants: with (+S9) and without (-S9) enzymatic activation. Only one compound 3,4-di(4'-methylphenyl)thiophene showed mutagenic activity when studied with metabolic activation (+S9) and its mutagenic rate (MR) score was 3.41 for the dose of 10.00µg*plate-1. Other studied compounds did not show any mutagenic activity (+/-S9) and their MR score did not exceed the threshold value of 2.0.
    Źródło:
    Environmental Biotechnology; 2011, 7, 2; 65-69
    1734-4964
    Pojawia się w:
    Environmental Biotechnology
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    The Ames test: a methodological short review
    Autorzy:
    Tejs, S.
    Powiązania:
    https://bibliotekanauki.pl/articles/363234.pdf
    Data publikacji:
    2008
    Wydawca:
    Uniwersytet Warmińsko-Mazurski w Olsztynie
    Tematy:
    Salmonella
    test Amesa
    mutagenność
    krótkoterminowe testy in-vitro
    Ames test
    mutagenicity
    short term in-vitro tests
    Opis:
    The Ames Salmonella/microsome mutagenicity assay (Salmonella test; Ames test) is a short-term bacterial reverse mutation assay specifically designed to detect a wide range of chemical substances that can produce genetic damage that leads to gene mutations. The test is used to evaluate the mutagenic properties of test articles. The Ames test uses amino acid-dependent strains of Salmonella typhimurium and Escherichia coli, each carrying different mutations in various genes in the histidine operon. These mutations act as hot spots for mutagens that cause DNA damage via different mechanisms. In the absence of an external histidine source, cells cannot grow and form colonies. Only those bacteria that revert to histidine independence (his+) are able to form colonies. The number of spontaneously induced revertant colonies per plate is relatively constant. However, when a mutagen is added to the plate, the number of revertant colonies per plate is increased, usually in a dose-related manner. The Ames test is used worldwide as an initial screen to determine the mutagenic potential of new chemicals and drugs. The purpose of this publication is to help researchers who apply the Ames test in their studies.
    Źródło:
    Environmental Biotechnology; 2008, 4, 1; 7-14
    1734-4964
    Pojawia się w:
    Environmental Biotechnology
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Preliminary evaluation of mutagenic activity of two amino derivatives of cyclopenta[c]phenanthrene
    Autorzy:
    Łuczyński, M. K.
    Góra, M.
    Mielżyńska, D.
    Brzuzan, P.
    Tejs, S.
    Pirogowicz, P.
    Luczynski, M.
    Powiązania:
    https://bibliotekanauki.pl/articles/363278.pdf
    Data publikacji:
    2007
    Wydawca:
    Uniwersytet Warmińsko-Mazurski w Olsztynie
    Tematy:
    test Amesa
    fenantren
    mutacje DNA
    WWA
    Ames test
    phenanthrene
    DNA mutations
    PAHs
    Opis:
    The mutagenicity of two, newly synthesized amino-derivatives of cyclopenta[c]phenanthrene (CP[c]Ph) was investigated in the Ames test using TA98 and TA100 histidine dependent Salmonella typhimurium strains. Neither of the examined compounds showed mutagenic activity without metabolic activation. The incorporation of an activation system caused high mutagenicity of CP[c]Ph derivatives in both S. typhimurium strains. The results are compared with the previous data on the mutagenic activity of unsubstituted CP[c]Ph and possible mechanisms of activation are discussed.
    Źródło:
    Environmental Biotechnology; 2007, 3, 1; 15-19
    1734-4964
    Pojawia się w:
    Environmental Biotechnology
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Biodegradacja polichlorowanych bifenyli przez zespół mikroorganizmów wyizolowanych z wody i gleby
    Biodegradation of polychlorinated biphenyls with microorganisms isolated from water and soil
    Autorzy:
    Piekarska, K.
    Powiązania:
    https://bibliotekanauki.pl/articles/236580.pdf
    Data publikacji:
    2003
    Wydawca:
    Polskie Zrzeszenie Inżynierów i Techników Sanitarnych
    Tematy:
    polichlorowane bifenyle
    biodegradacja PCB
    test Amesa
    właściwości mutagenne
    polychlorinated biphenyls
    PCBs biodegradation
    Ames test
    mutagenic properties
    Opis:
    Praca przedstawia rezultaty badań dotyczące możliwości biodegradacji chloroorganicznych składników preparatu technicznego Aroclor 1242. Z wody i gleby skażonej związkami chloroorganicznymi wyizolowano zespół mikroorganizmów składający się z dziewięciu aktywnych szczepów zdolnych do rozkładu polichlorowanych bifenyli (PCB). Szczepy te należały do następujących rodzajów: Micrococcus, Alcaligenes, Arthrobacter i Pseudomonas, Rhodotorula i Candida. Następnie zbadano przydatność pozyskanych drobnoustrojów do rozkładu składników Arocloru 1242 w hodowlach pojedynczych szczepów i ich mieszaniny. Postęp biodegradacji badano metodami mikrobiologicznymi, biochemicznymi i chemicznymi. Do oznaczeń zawartości PCB zastosowano chromatograf gazowy z selektywnym (względem związków chloroorganicznych) dedektorem ECD. Zbadano także potencjalne właściwości mutagenne produktów pobiodegradacyjnych metodą testu Amesa. Wszystkie badane mikroorganizmy odznaczały się dobrymi właściwościami biodegradacyjnymi, rozkładając PCB z wydajnością 5÷60%. Największą wydajność rozkładu preparatu Aroclor 1242, około 72%, uzyskano w przypadku zastosowania mieszanej kultury, a nie pojedynczych aktywnych szczepów. Badania biochemiczne wykazały wysoką aktywność biologiczną szczepów podczas trwania procesu rozkładu PCB, co potwierdziło wyniki badań analitycznych. Badania genotoksyczności powstających metabolitów biodegradacji, wykonane testem Amesa, wykazały brak obecności związków o charakterze mutagennym i rakotwórczym w większości ekstraktów pobiodegradacyjnych.
    Owing to their high toxicity, strong bioaccumulation and low degradability, chloroorganic compounds raise serious ecological problems. The objective of the study reported in this paper was to degrade polychlorinated biphenyls (PCBs), the components of the commercial Aroclor 1242 mixture, with microorganism cultures isolated from chlororganic-contaminated water and soil. There were nine strains, the majority of which belonged to the genera Micrococus, Alcaligenes, Arthrobacter, Pseudomonas, Rhodotorula and Candida. The taxonomic status of the isolated strains and their ability to degrade PCB congeners were determined. The biodegradation of the Aroclor 1242 components was monitored using microbiological, biochemical and chemical methods. PCB concentrations were determined by gas chromatography (GC-ECD). Potential mutagenic and carcinogenic properties of the biodegradation products were examined with the Ames test. The PCB congeners were degraded by selected bacterial strains with an efficiency which ranged from 5% to 60%. The highest efficiency (72%) was achieved with a mixed culture. Bioindication studies showed that, in the majority of instances, no mutagenic metabolites were produced in the course of the biodegradation process.
    Źródło:
    Ochrona Środowiska; 2003, R. 25, nr 2, 2; 21-27
    1230-6169
    Pojawia się w:
    Ochrona Środowiska
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
      Wyświetlanie 1-8 z 8

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