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    Wyświetlanie 1-13 z 13
    Tytuł:
    Agrobacterium -mediated transformation of tobacco and sorghum with a genetic construct containing genes encoding enzymes in the sucrose synthesis pathway
    Autorzy:
    Marszalek, M.
    Zeyland, J.
    Luwanska, A.
    Wielgus, K.
    Slomski, R.
    Lipinski, D.
    Powiązania:
    https://bibliotekanauki.pl/articles/951210.pdf
    Data publikacji:
    2015
    Wydawca:
    Polska Akademia Nauk. Czytelnia Czasopism PAN
    Tematy:
    conference
    Agrobacterium
    Agrobacterium tumefaciens
    transformation
    tobacco
    sorghum
    gene encoding
    enzyme
    sucrose
    Źródło:
    BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology; 2015, 96, 1
    0860-7796
    Pojawia się w:
    BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Odyssey of Agrobacterium T-DNA.
    Autorzy:
    Ziemienowicz, Alicja
    Powiązania:
    https://bibliotekanauki.pl/articles/1044091.pdf
    Data publikacji:
    2001
    Wydawca:
    Polskie Towarzystwo Biochemiczne
    Tematy:
    Agrobacterium
    DNA transfer
    T-DNA
    virulence
    Opis:
    Agrobacterium tumefaciens, a plant pathogen, is characterized by the unique feature of interkingdom DNA transfer. This soil bacterium is able to transfer a fragment of its DNA, called T-DNA (transferred DNA), to the plant cell where T-DNA is integrated into the plant genome leading to "genetic colonization" of the host. The fate of T-DNA, its processing, transfer and integration, resembles the journey of Odysseus, although our hero returns from its long trip in a slightly modified form.The soil bacterium, Agrobacterium tumefaciens, is a plant pathogen responsible for tumor induction on dicotyledonous plants due to its ability to transfer DNA to the plant cell (reviewed in: de la Cruz & Lanka, 1998; Gelvin, 2000; Hansen & Chilton, 1999; Lartey & Citovsky, 1997; Rossi et al., 1998; Zupan & Zambryski, 1997). In biotechnology this ability is widely used for plant transformation. During tumor induction Agrobacterium attaches to plant cells and then transfers part of its DNA to some of these cells. The transferred DNA (T-DNA) which resides on a large Ti (tumor inducing) plasmid, is processed within the bacterium and is exported to the plant where it becomes integrated into the plant genome (reviewed in: Sheng & Citovsky, 1996; Tinland & Hohn, 1995; Tinland, 1996). Proteins encoded by the virulence (vir) region of the Ti plasmid regulate T-DNA processing and transfer. Phenolic compounds derived from a wounded plant cell wall induce expression of the vir region genes. Virulence proteins recognize 25 bp imperfect direct repeats (border sequences) that define the T-DNA. In the presence of VirD1 protein, VirD2 cleaves the border sequence in a site- and strand-specific manner and subsequently becomes covalently attached to the 5' end of the nicked DNA. The nicked DNA is then displaced from the plasmid producing single-stranded T-DNA. The T-DNA-VirD2 complex and the VirE2 protein are believed to be transferred to the plant through a pilus-like structure containing VirB and VirD4 proteins. In the plant cell, T-DNA becomes coated with the single-stranded DNA-binding protein, VirE2. The T-DNA-protein complex is imported into the nucleus where the T-DNA is integrated into the nuclear genome. Expression of genes located on T-DNA leads to the formation of proteins involved in the production of auxins and cytokinins. These plant hormones cause the tumorous phenotype that is characterized by the ability of the plant cells to proliferate limitlessly and autonomously even in the absence of added phytohormones. Crown gall tumors are characterized by the production of opines (amino-acid derivatives). The biosynthesis of opines is catalyzed by opine synthases, which are encoded by the T-DNA. Opines formed in the tumors can be metabolized by the tumorigenic agrobacteria, but not by most of the other soil organisms. Thus, Agrobacterium creates for itself a favorable niche by genetic modification of plant cells, a process called "genetic colonization". All stages of this colonization, including chemotaxis, attachment, induction of virulence region, processing of T-DNA, T-DNA transfer, T-DNA integration, expression of T-DNA genes and changes in the plant phenotype, will be discussed in the following chapters. This will be an odyssey of T-DNA that leaves the Agrobacterium cell in the form of nucleic acid and returns from its journey in the form of opines, derivatives of amino acids (Fig. 1).
    Źródło:
    Acta Biochimica Polonica; 2001, 48, 3; 623-635
    0001-527X
    Pojawia się w:
    Acta Biochimica Polonica
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Production of oleanolic acid glycosides by hairy root established cultures of Calendula officinalis L.
    Autorzy:
    Długosz, Marek
    Wiktorowska, Ewa
    Wiśniewska, Anita
    Pączkowski, Cezary
    Powiązania:
    https://bibliotekanauki.pl/articles/1039552.pdf
    Data publikacji:
    2013
    Wydawca:
    Polskie Towarzystwo Biochemiczne
    Tematy:
    Marigold
    Agrobacterium rhizogenes
    hairy roots
    triterpenic saponins
    oleanolic acid
    transformation
    Opis:
    In order to initiate hairy root culture initiation cotyledons and hypocotyls of Calendula officinalis L. were infected with Agrobacterium rhizogenes strain ATCC 15834 or the same strain containing pCAMBIA 1381Z vector with β-glucuronidase reporter gene under control of promoter of NIK (Nematode Induced Kinase) gene. The efficiency of induction of hairy roots reached 33.8% for cotyledons and 66.6% for hypocotyls together for both transformation experiments. Finally, eight control and nine modified lines were established as a long-term culture. The hairy root cultures showed the ability to synthesize oleanolic acid mainly (97%) as glycosides; control lines contained it at the average 8.42 mg · g-1 dry weight in tissue and 0.23 mg · dm-3 in medium; modified lines: 4.59 mg · g-1 for the tissue, and 0.48 mg · dm-3 for the medium. Additionally lines showed high positive correlation between dry/fresh weight and oleanolic acid concentration in tissue. Using the Killiani mixture in acidic hydrolysis of oleanolic acid glycosides released free aglycones that were partially acetylated in such conditions.
    Źródło:
    Acta Biochimica Polonica; 2013, 60, 3; 467-473
    0001-527X
    Pojawia się w:
    Acta Biochimica Polonica
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Optimization of transient Agrobacterium-mediated gene expression system in leaves of Nicotiana benthamiana
    Autorzy:
    Wydro, Mateusz
    Kozubek, Edward
    Lehmann, Przemysław
    Powiązania:
    https://bibliotekanauki.pl/articles/1041239.pdf
    Data publikacji:
    2006
    Wydawca:
    Polskie Towarzystwo Biochemiczne
    Tematy:
    RNA silencing
    Nicotiana benthamiana
    viral suppressor
    Agrobacterium-mediated transient expression
    Opis:
    Here we report on a simple and reproducible system of Agrobacterium-mediated transient gene expression assay that utilizes infiltration of young Nicotiana benthamiana leaves. Although some of the phenomena described in this paper have been already reported by other researchers, here we have further developed them. The highest level of transient gfp gene expression was detected in the youngest leaves of N. benthamiana infiltrated with A. tumefaciens strains AGL0 and EHA105 precultured in the presence of 450-600 µM acetosyringone. Although the maximum level of transient gfp gene expression was restricted presumably by RNA silencing, it was completely suppressed in the presence of the viral protein HC-Pro. The transient expression system described here can be used to identify new viral suppressors of RNA silencing, for detailed analysis of unidentified genes and for industrial production of proteins in plants as well.
    Źródło:
    Acta Biochimica Polonica; 2006, 53, 2; 289-298
    0001-527X
    Pojawia się w:
    Acta Biochimica Polonica
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Transformation of wild Solanum species resistant to late blight by using reporter gene gfp and msh2 genes
    Autorzy:
    Rakosy-Tican, Lenuta
    Aurori, Adriana
    Aurori, Cristian M.
    Ispas, Gabriela
    Famelaer, Ivan
    Powiązania:
    https://bibliotekanauki.pl/articles/2198986.pdf
    Data publikacji:
    2004-12-20
    Wydawca:
    Instytut Hodowli i Aklimatyzacji Roślin
    Tematy:
    Agrobacterium tumefaciens mediated transformation
    DNA mismatch repair
    gfp
    nptII marker gene
    Opis:
    Green fluorescent protein (gfp) reporter gene and nptII marker gene were used to optimize Agrobacterium tumefaciens (agro) mediated transformation of wild Solanum genotypes resistant to late blight. Different genotypes of Solanum bulbocastanum, S. chacoense, S. microdontum and S. verrucosum were assessed for their regeneration ability on MS based media and for agro-mediated transformation. As the first step reporter genes were used to optimize transformation protocol for each species and then the transfer of genes involved in mismatch repair of DNA were attempted in Solanum chacoense. For transformation, either leaf or stem fragments were used. It was shown that gfp is a valuable and elegant tool for monitoring the efficiency of transformation or the occurrence of chimera in all genotypes. Transformation efficiency was dependent on a plant genotype. A number of genotypes have been successfully transformed and they expressed constitutively the bright green fluorescence of gfp without any side effects. The most recalcitrant species proved to be S. microdontum, which did not regenerate plants although different media and phytohormones had been used. The best protocol for S. chacoense transformation was also found to work in the transfer of msh2 genes. Msh2 isolated from Arabidopsis was used and transferred either as mutated (Apa) or antisense (As) gene. The integration of msh2-mutated gene into S. chacoense genome was demonstrated by PCR amplification and confirmed by RT-PCR for some of the putative transgenic clones. The implications of mismatch repair in homologous recombination and its importance for potato improvement are discussed. 
    Źródło:
    Plant Breeding and Seed Science; 2004, 50; 119-127
    1429-3862
    2083-599X
    Pojawia się w:
    Plant Breeding and Seed Science
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Improved transformation of Agrobacterium assisted by silver nanoparticle
    Autorzy:
    Benny, Amala
    Alex, Swapny
    Soni, K.B.
    Anith, K.N.
    Kiran, A.G.
    Viji, M.M.
    Powiązania:
    https://bibliotekanauki.pl/articles/16648111.pdf
    Data publikacji:
    2022
    Wydawca:
    Polska Akademia Nauk. Czasopisma i Monografie PAN
    Tematy:
    silver nanoparticles
    Agrobacterium transformation
    calcium chloride
    competent cells
    freeze thaw
    transformation efficiency
    Opis:
    In transgenic plant development, the low transformation efficiency of Agrobacterium with exogenous DNA is the major constraint, and hence, methods to improve its transformation efficiency are needed. Recently, nanoparticlemediated gene transfer has evolved as a key transformational tool in genetic transformation. Since silver nanoparticles (AgNPs) can induce pores on the cell membrane, their efficacy in the improvement of conventional calcium chloride freeze-thaw technique of transformation of Agrobacterium was explored in this study. Agrobacterium cells in the exponential growth phase were exposed to different concentrations of AgNPs (0.01, 1, 5, 10, and 20 mg/l), and the half-maximal effective concentration (EC50) was determined via Probit analysis using the SPSS software. Transformation efficiency of AgNPs alone and in combination with calcium chloride was compared with that of the conventional calcium chloride freeze-thaw technique. AgNPs at a concentration of 0.01 mg/l in combination with calcium chloride (20 mM) showed a ten fold increase in the transformation efficiency (3.33 log CFU (colony-forming unit/microgram of DNA) of Agrobacterium tumefaciens strain EHA 105 with plasmid vector pART27 compared with the conventional technique (2.31 log CFU/μg of DNA). This study indicates that AgNPs of size 100 nm can eliminate the freeze-thaw stage in the conventional Agrobacterium transformation technique, with a 44% improvement in efficiency. The use of AgNPs (0.01 mg/l) along with 20 mM calcium chloride was found to be an economically viable method to improve the transformation of Agrobacterium with exogenous plasmid DNA.
    Źródło:
    BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology; 2022, 103, 3; 311-317
    0860-7796
    Pojawia się w:
    BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Agrobacterium-mediated genetic transformation of Miscanthus sinensis and Miscanthus × giganteus. Preliminary studies
    Autorzy:
    Cerazy, J.
    Slusarkiewicz-Jarzina, A.
    Ponitka, A.
    Malec, K.
    Jezowski, S.
    Pniewski, T.
    Powiązania:
    https://bibliotekanauki.pl/articles/951241.pdf
    Data publikacji:
    2015
    Wydawca:
    Polska Akademia Nauk. Czytelnia Czasopism PAN
    Tematy:
    conference
    Agrobacterium
    genetic transformation
    Miscanthus sinensis
    biomass
    renewable raw material
    bioenergy
    biofuel
    plant transformation
    Źródło:
    BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology; 2015, 96, 1
    0860-7796
    Pojawia się w:
    BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Norway spruce (Picea abies) genetic transformation with modified Cry3A gene of Bacillus thuringiensis
    Autorzy:
    Bříza, Jindřich
    Pavingerová, Daniela
    Vlasák, Josef
    Niedermeierová, Hana
    Powiązania:
    https://bibliotekanauki.pl/articles/1039537.pdf
    Data publikacji:
    2013
    Wydawca:
    Polskie Towarzystwo Biochemiczne
    Tematy:
    Cry3A gene modification
    Picea abies
    Agrobacterium tumefaciens and biolistic transformation
    embryogenic tissue
    somatic embryogenesis
    Opis:
    Modified versions of the Cry3A gene of Bacillus thuringiensis (Bt) were transferred into Norway spruce (Picea abies). Both the biolistic approach and Agrobacterium tumefaciens mediated procedure were employed for transformation of embryogenic tissue (ET) cultures. The latter method proved to be more efficient yielding 70 transgenic embryogenic tissue lines compared with 18 lines obtained by biolistics. The modified Cry3A genes were driven by a 35S promoter and the nptII screenable selection marker gene was used in all vectors. The transgenic ETs were molecularly characterized and converted into mature somatic embryos. Germinating embryos formed plantlets which were finally planted into perlite and their Cry3A gene transcription activities were demonstrated by RT-PCR.
    Źródło:
    Acta Biochimica Polonica; 2013, 60, 3; 395-400
    0001-527X
    Pojawia się w:
    Acta Biochimica Polonica
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Expression of hepatitis B core antigen in lettuce and tobacco for advanced plant-derived vaccine formulations
    Autorzy:
    Pyrski, M.
    Kasprzyk, A.
    Bogusiewicz, M.
    Bociag, P.
    Czyz, M.
    Pniewski, T.
    Powiązania:
    https://bibliotekanauki.pl/articles/951208.pdf
    Data publikacji:
    2015
    Wydawca:
    Polska Akademia Nauk. Czytelnia Czasopism PAN
    Tematy:
    conference
    hepatitis B virus
    antigen expression
    virus-like particle
    transgenic tobacco
    lettuce plant
    Agrobacterium tumefaciens
    immunoblot
    ELISA test
    Źródło:
    BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology; 2015, 96, 1
    0860-7796
    Pojawia się w:
    BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Transformacja rzepaku genem reporterowym kodujacym bialko zielonej fluorescencji za pomoca Agrobacterium tumefactens.
    Autorzy:
    Troczynska, J
    Flasinski, S.
    Drozdowska, L.
    Powiązania:
    https://bibliotekanauki.pl/articles/833820.pdf
    Data publikacji:
    2001
    Wydawca:
    Instytut Hodowli i Aklimatyzacji Roślin
    Tematy:
    Agrobacterium tumefaciens
    fluorescencja chlorofilu
    rzepak
    bialko
    transformacja
    genetyka roslin
    regeneracja pedow
    chlorophyll fluorescence
    rape
    protein
    genetic transformation
    shoot regeneration
    Źródło:
    Rośliny Oleiste - Oilseed Crops; 2001, 22, 2; 569-578
    1233-8273
    Pojawia się w:
    Rośliny Oleiste - Oilseed Crops
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    The effect of L-glutamine on the genetic transformation of embryogenic cell suspensions of gentian species (Gentiana lutea L., Gentiana cruciata L., and Gentiana kurroo Royle) using Agrobacterium tumefaciens
    Autorzy:
    Rybczynski, J.J.
    Wojcik, A.I.
    Powiązania:
    https://bibliotekanauki.pl/articles/80392.pdf
    Data publikacji:
    2019
    Wydawca:
    Polska Akademia Nauk. Czytelnia Czasopism PAN
    Tematy:
    embryogenic cell
    gentian
    Gentiana lutea
    Gentiana cruciata
    Gentiana kurroo
    Agrobacterium tumefaciens
    kanamycin resistance
    L-alanyl-L-glutamine
    genetic transformation
    Źródło:
    BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology; 2019, 100, 1
    0860-7796
    Pojawia się w:
    BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Podatnosc odmian chryzantemy [Dendranthema grandiflora Tzvelev] na guzowatosc Agrobacterium tumefaciens [Smith et Townsend] Conn
    Susceptibility of chrysanthemum [Dendranthema grandiflora Tzvelev] cultivars to crown gall [Agrobacterium tumefaciens [Smith et Townsend] Conn]
    Autorzy:
    Schollenberger, M
    Powiązania:
    https://bibliotekanauki.pl/articles/28002.pdf
    Data publikacji:
    2005
    Wydawca:
    Polskie Towarzystwo Botaniczne
    Tematy:
    Agrobacterium tumefaciens
    Dendranthema grandiflora
    chryzantema wielkokwiatowa
    czynniki chorobotworcze
    choroby bakteryjne
    bakterie chorobotworcze
    grupy upraw
    podatnosc na choroby
    guzowatosc
    odmiany roslin
    mother chrysanthemum
    pathogenic factor
    bacterial disease
    pathogenic bacteria
    disease susceptibility
    crown gall disease
    plant cultivar
    Opis:
    The susceptibility of 29 cultivars of chrysanthemum, representing various types of growth, to crown gall was studied. Two isolates of Agrobacterium tumefaciens: chrysanthemum and apple were used for testing plants. The inoculation was made to the stem base of rooted cuttings and places of pinching off. The first method gave larger galls on most tested plants and only three cultivars were resistant to inoculation with both A. tumefaciens isolates. Weak symptoms were obtained after cutting of stem tips, thus plants of 9 and 5 cultivars (in subsequent tests) showed no symptoms. Only three chrysanthemum cultivars were resistant to crown gall in all tests: Epidote Jaune, Epidote Orange and Epidote Rouge, all belonging to potted chrysanthemum. Plants of Maracas cultivar were resistant when bacteria were applied to the wound of stem tips. In all tests, the apple isolate was weak pathogenic, thus no gall were observed on many cultivars.
    Na podstawie doświadczeń szklarniowych oceniono podatność 29 odmian chryzantemy, reprezentujących pięć grup uprawowych od wielkokwiatowych po doniczkowe drobnokwiatowe, na guzowatość. Sadzonki zakażano dwoma izolatami chryzantemowym i jabłoniowym Agrobacterium tumefaciens inokulując podstawę łodygi jak i część wierzchołkową. Po zastosowaniu pierwszej metody uzyskiwano silniejsze objawy i tylko rośliny trzech odmian (grupa Epidote, drobnokwiatowe) nie uległy porażeniu. Inokulując części szczytowe sadzonek uzyskano słabsze objawy chorobowe i przy tej metodzie 9 i 5 odmian (zależnie od doświadczenia) nie uległo porażeniu. W każdym przypadku odporne okazały się trzy odmiany: Epidote Jaune, Epidote Orange, Epidote Rouge. Przy tej metodzie dwukrotnie nie uległy porażeniu rośliny odmiany Maracas (doniczkowa wielkokwiatowa). We wszystkich przeprowadzonych doświadczeniach stwierdzono istotne różnice w patogeniczności izolatów jabłoniowego i chryzantemowego w stosunku do zakażanych roślin.
    Źródło:
    Acta Agrobotanica; 2005, 58, 2; 485-496
    0065-0951
    2300-357X
    Pojawia się w:
    Acta Agrobotanica
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Efficacy of biochemical preparations and extract from Hypericum perforatum against bacterial diseases
    Autorzy:
    Schollenberger, M.
    Pudło, S.
    Paduch-Cichal, E.
    Mirzwa-Mróz, E.
    Powiązania:
    https://bibliotekanauki.pl/articles/12688150.pdf
    Data publikacji:
    2019
    Wydawca:
    Uniwersytet Przyrodniczy w Lublinie. Wydawnictwo Uniwersytetu Przyrodniczego w Lublinie
    Tematy:
    plant protection
    integrated plant protection
    plant preparation
    biochemical preparation
    biotechnical preparation
    plant extract
    Biosept Active preparation
    BioZell preparation
    herbal plant
    St.John's wort
    Hypericum perforatum
    application effect
    antimicrobial activity
    bacterial disease
    pathogenic bacteria
    Agrobacterium tumefaciens
    Pseudomonas syringae pv.syringae
    Xanthomonas arboricola pv.corylina
    Opis:
    The biotechnical preparations: Biosept Active (based on a grapefruit extract) and BioZell (based on thyme oil) as well as Hypericum perforatum extract, streptomycin solution and fungicide Champion 50WP (active ingredient substance – e.i. 50% copper hydroxide) were investigated for antimicrobial effects against plant pathogenic bacteria: Agrobacterium tumefaciens, Pseudomonas syringae pv. syringae and Xanthomonas arboricola pv. corylina. The screening was carried out in vitro on three media: Nutrient Agar (NA Difco), Pseudomonas Agar F (Merck) – analogue of King B and 523. In the experiments, the agar plate method was applied. There were no statistically significant differences in the effect of streptomycin and Champion 50WP on the growth inhibition of three bacteria strains for medium 523 and Nutrient Agar and of P. syringae pv. syringae and X. arboricola pv. corylina for medium King B. It was determined that the antibacterial activity of Biosept Active and BioZell biopreparations and H. perforatum extract against Agrobacterium tumefaciens (strain C58), Pseudomonas syringae pv. syringae (strain 760) and Xanthomonas arboricola pv. corylina (strain RIPF-x13) were dependent on the strain of pathogen as well as the growth medium used. According to the research results obtained, the Biosept Active preparation and H. perforatum extract demonstrated high bacteriostatic activity against three bacterial strains grown on the Nutrient Agar medium.
    Źródło:
    Acta Scientiarum Polonorum. Hortorum Cultus; 2019, 18, 3; 147-156
    1644-0692
    Pojawia się w:
    Acta Scientiarum Polonorum. Hortorum Cultus
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
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