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Wyświetlanie 1-15 z 299
Tytuł:
SpaCBA sequence instability and its relationship to the adhesion efficiency of Lactobacillus casei group isolates to Caco-2 cells
Autorzy:
Markowicz, Corinna
Olejnik-Schmidt, Agnieszka
Borkowska, Monika
Schmidt, Marcin
Powiązania:
https://bibliotekanauki.pl/articles/1039301.pdf
Data publikacji:
2014
Wydawca:
Polskie Towarzystwo Biochemiczne
Tematy:
Lactobacillus
adhesion
spaCBA
Opis:
The ability to adhere to enterocytes is one of the key features of probiotics. This process involves a number of factors, among which the important role of pili was demonstrated. Some Lactobacillus species are confirmed to have heterotrimeric spaCBA type pili. The aim of this study was to identify spaCBA pili in strains of selected Lactobacillus spp. and assess the impact of their presence and sequence polymorphism on the adhesion of these strains to enterocytes. Total 20 bacterial strains of L. rhamnosus, L. casei and L. paracasei were tested. The presence of pilus specific proteins coding genes spaA, spaB and spaC was verified by PCR in order to identify the presence of sequence polymorphism in the genes possibly affecting the structure of the spaCBA pilus. To correlate spaCBA polymorphism to adhesion capability the adhesion assay was carried out using Caco-2 cell line. The effectiveness of the adhesion was measured using a scintillation counter. The Lactobacillus strains analyzed showed the adhesion to Caco-2 enterocytes capability from 0.6% to 19.6%. The presence of spaCBA pili is a factor increasing the adhesion efficiency of Lactobacillus spp. to Caco-2 enterocytes. Lack of these structures on the surface of bacterial cells results in the reduction in adhesion efficiency, indicating its important role in the adhesion process. But not in all cases the correlation between the presence of protein spaCBA structures and adhesion efficiency was observed, what may indicate the important role of other factors in adhesion of analyzed strains to Caco-2 cells.
Źródło:
Acta Biochimica Polonica; 2014, 61, 2; 341-347
0001-527X
Pojawia się w:
Acta Biochimica Polonica
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Influence of surface shape of glued anchors on their load capacity
Autorzy:
Baryłka, Adam
Tomaszkiewicz, Dariusz
Powiązania:
https://bibliotekanauki.pl/articles/132031.pdf
Data publikacji:
2020
Wydawca:
Centrum Rzeczoznawstwa Budowlanego Sp. z o.o.
Tematy:
anchors
adhesion stress
Opis:
The surface shape of the anchorages and their effect on the adhesion stress were analyzed. Anchors made of plain and threaded rods were put together. It was assessed whether a smooth bar in this type of anchorages could ful fill the actual role of a connector. Groups of anchors in various anchor systems were put together and smooth anchorages compared to threaded anchors. Four types of diameters were considered: 8 mm, 10 mm, 12 mm and 20 mm.
Źródło:
Modern Engineering; 2020, 2; 78-82
2450-5501
Pojawia się w:
Modern Engineering
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Expression of EpCAM protein in gastric cancer cells may contribute to its histogenesis
Autorzy:
Nizioł, M.
Kuczyńska, P.
Misiura, M.
Zińczuk, J.
Zaręba, K.
Ustymowicz, W.
Ćwiklińska-Dworakowska, M.
Baszun, M.
Jelski, S.
Guzińska-Ustymowicz, K.
Pryczynicz, A.
Powiązania:
https://bibliotekanauki.pl/articles/1918619.pdf
Data publikacji:
2018-06-18
Wydawca:
Uniwersytet Medyczny w Białymstoku
Tematy:
Adhesion
EpCAM
gastric cancer
Opis:
Introduction: EpCAM protein belongs to adhesion molecules of epithelial cells. It mediates in the homophile adhesion cell-cell reactions. EpCAM protein expression can be observed in the majority of healthy normal cells. However, mutations in EpCAM gene may lead to an increased risk of cancer development. The aim of the study was to assess EpCAM protein expression in the correlation with chosen clinical and histological parameters in gastric cancer. Materials and Methods: EpCAM protein expression was evaluated immunohistochemically in 88 patients diagnosed with gastric cancer. Results: An increase in EpCAM protein expression was demonstrated in cancer cells compared to normal gastric mucosa (59.3% cancers with the positive expression of EpCAM protein). The increased EpCAM protein expression was observed in patients with a histological type of adenocarcinoma without a mucinous component than in those with adenocarcinoma with a mucinous component (p=0.028). The higher expression of this protein was observed also in the intestinal type according to the Lauren classification (p=0.037). The expression of the protein was lower in the diffuse type of cancer. Additionally, an increase in EpCAM protein expression was revealed in cancers infiltrating to the blood vessels (p=0.013). Conclusions: A correlation between EpCAM expression and adenocarcinoma without a mucinous component as well as the intestinal type according to the Lauren classification may prove a role of this protein in the histogenesis of gastric cancer. Moreover, its positive expression is related to cancerous cells infiltrating to the blood vessels, which may suggest a role of EpCAM protein in the early stages of gastric cancer metastases.
Źródło:
Progress in Health Sciences; 2018, 8(1); 74-79
2083-1617
Pojawia się w:
Progress in Health Sciences
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Cell adhesion molecules and their possible role in the pathology of the peripheral nerves – a review of the literature
Autorzy:
Niezgoda, Adam
Powiązania:
https://bibliotekanauki.pl/articles/552657.pdf
Data publikacji:
2015
Wydawca:
Stowarzyszenie Przyjaciół Medycyny Rodzinnej i Lekarzy Rodzinnych
Tematy:
cell adhesion molecules
demyelination
polyneuropathies.
Źródło:
Family Medicine & Primary Care Review; 2015, 4; 323-326
1734-3402
Pojawia się w:
Family Medicine & Primary Care Review
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Monitoring of the kinetics of adhesive bond type car putty coating-steel substrate with ultrasonic method
Monitorowanie kinetyki tworzenia połączenia adhezyjnego typu powłoka szpachlówkowa-podłoże stalowe metodą ultradźwiękową
Autorzy:
Ulbrich, D.
Jósko, M.
Mańczak, R.
Powiązania:
https://bibliotekanauki.pl/articles/335203.pdf
Data publikacji:
2013
Wydawca:
Sieć Badawcza Łukasiewicz - Przemysłowy Instytut Maszyn Rolniczych
Tematy:
ultrasound
adhesion
car putty coatings
Opis:
W artykule przedstawiono metodę monitorowania konstytuowania połączenia adhezyjnego typu powłoka szpachlówkowapodłoże stalowe. Badania wykonano na próbkach stalowych. Celem badań było wyznaczenie, na podstawie zmiany wysokości impulsu ultradźwiękowego, trendu zmiany wzmocnienia sygnału i modułu ciśnieniowego współczynnika odbicia. Wyniki zobrazowano na wykresach; przedstawiono wartości maksymalne, minimalne i średnią wyżej wymienionych parametrów w czasie utwardzania połączenia, który wynosił 60 minut, a zmiany wysokości pierwszego echa sygnału ultradźwiękowego zapisywano co 3 sekundy. W kolejnym etapie badań zostanie zastosowana inna powłoka szpachlówkowa, a otrzymane wyniki będą porównane z zaprezentowanymi w niniejszym artykule.
A method used for monitoring the formation process of adhesive bond type car putty coating and steel substrate is presented in this article. In the experiment some samples made of steel and universal car putty are used. The main goal of the experiment is to designate changes of two parameters: the variation of the ultrasonic gain and reflection coefficienvt during the cure time. These parameters are calculated based on the echo height changes of the ultrasonic signal. The test’s results: average, minimum and maximum values of determined parameters are shown in the figures. Time of the test is equal to 60 minutes and every three seconds the variation of echo height is recorded. The obtained results will be compared to the results for other car putties as the next step of the research.
Źródło:
Journal of Research and Applications in Agricultural Engineering; 2013, 58, 1; 189-192
1642-686X
2719-423X
Pojawia się w:
Journal of Research and Applications in Agricultural Engineering
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Effect of α3β1 and αvβ3 integrin glycosylation on interaction of melanoma cells with vitronectin
Autorzy:
Janik, Marcelina
Przybyło, Małgorzata
Pocheć, Ewa
Pokrywka, Małgorzata
Lityńska, Anna
Powiązania:
https://bibliotekanauki.pl/articles/1040422.pdf
Data publikacji:
2010
Wydawca:
Polskie Towarzystwo Biochemiczne
Tematy:
adhesion
integrin
glycosylation
migration
vitronectin
Opis:
The metastatic transformation of melanocytes is associated with altered expression of adhesion molecules, including αvβ3 and α3β1 integrins. Integrin αvβ3 is a primary vitronectin (VN) receptor, while both integrin types take part in adhesion to VN when they are in complex with uPAR. Although their role in melanoma cell interaction with VN is of great interest, the influence of N-oligosaccharides attached to these glycoproteins is still unappreciated. The present study assesses the role of αvβ3 and α3β1 integrins and the influence of their glycosylation status on WM9 and WM239 metastatic melanoma cell interactions with VN. Cell adhesion to and migration on VN were selected as the studied cell behaviour parameters. Functionblocking antibodies and swainsonine (SW) treatment were used in these tests. Both cell lines interacted with VN in an integrin-mediated but cell-line-specific manner. In WM9 cells, migration was not completely inhibited by antibodies against α3β1 or αvβ3 integrins, suggesting the participation of other VN receptors. In both cell lines in coprecipitation test the formation of an integrins/uPAR complex was shown. In the presence of SW formation of the complex did not occur, suggesting the participation of glycosylation in this proccess. Additionally, the adhesion properties of WM9 cells were changed after SW treatment. Our results suggest that in these two metastatic cell lines integrin-linked N-oligosaccharides influence the VN adhesion receptor activity and function.
Źródło:
Acta Biochimica Polonica; 2010, 57, 1; 55-61
0001-527X
Pojawia się w:
Acta Biochimica Polonica
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Adhesive and hydrophobic properties of the selected LAB isolated from gastrointestinal tract of farming animals
Autorzy:
Grajek, Katarzyna
Sip, Anna
Foksowicz-Flaczyk, Joanna
Dobrowolska, Anna
Wita, Agnieszka
Powiązania:
https://bibliotekanauki.pl/articles/1038819.pdf
Data publikacji:
2016
Wydawca:
Polskie Towarzystwo Biochemiczne
Tematy:
lactic acid bacteria
adhesion
hydrophobicity
Opis:
The aim of this study was to determine the degree of adhesion and hydrophobicity of some strains of lactic acid bacteria (LAB) with proven antagonistic properties against pathogenic bacteria. Studies were performed using-LAB strains isolated from the gastrointestinal tract of calves and piglets. These strains exhibited an antibacterial activity against pathogenic strains of Clostridium perfringens and Escherichia coli. Cell adhesiveness was examined in relation to the porcine and bovine mucin. Our analyses had shown that the tested microorganisms demonstrated a degree of adhesion in the range of 32.00-40.00% for strains isolated from calves, and 34.00-40.00% for strains isolated from pigs. The hydrophobicity of tested bacteria was in the range of 31.00%-44.00% for strains isolated from pigs, and 26.00%-42.00% for strains obtained from calves. The best mucin adhesion ability was found for bacterial strains belonging to the Enterococcus genus isolated from calves. Taking into account porcine isolates, the best adhesion was observed for the Leuconostoc bacterial strains. Among tested strains, the highest hydrophobicity, measured in relation to hexadecane, was recorded for the bacterial strains belonging to the Leuconostoc sp. isolated from piglets and a of Lactobacillus sp. bacterial strain isolated from calves.
Źródło:
Acta Biochimica Polonica; 2016, 63, 2; 311-314
0001-527X
Pojawia się w:
Acta Biochimica Polonica
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Material Factors in Relation to Development Time in Liquid-Penetrant Inspection. Part 1. Material Factors
Autorzy:
Irek, P.
Słania, J.
Powiązania:
https://bibliotekanauki.pl/articles/351076.pdf
Data publikacji:
2016
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
imperfection
defect
penetration testing
adhesion
Opis:
In technical publications and European Standards the development time (i.e. time of getting out of penetrant from a discontinuity to the material surface) in penetration testing is specified within the range of 10-30 minutes. In practice, however, it is seen , that it is closely connected with the material type and ranges from several minutes to 24 hours. In the literature, only interactions coming from the penetrant are described, whereas those from the material under testing, i.e. the influence of material factors on adhesion between penetrant and material, are not taken into consideration. In this connection, it has been described precisely in the paper the adhesion phenomenon and also it has been indicated the other factors affecting the development time. Recapitulating the adhesion theories presented in the paper, it can be formulated two fundamental circumstances which must occur that adhesion joint may be formed, namely: the approach of the particles of two solids to the distance less than 0,9 nm while the high attractive force occurring between molecules should be connected with possibly low potential energy of the bond formed in this way.
Źródło:
Archives of Metallurgy and Materials; 2016, 61, 2A; 509-514
1733-3490
Pojawia się w:
Archives of Metallurgy and Materials
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Material Factors in Relation to Development Time in Liquid-Penetrant Inspection. Part 2. Investigation Programme and Preliminary Tests
Autorzy:
Irek, P.
Słania, J.
Powiązania:
https://bibliotekanauki.pl/articles/353038.pdf
Data publikacji:
2016
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
imperfection
defect
penetration testing
adhesion
Opis:
The paper is the continuation of the previous one entitled “Material factors in relation to development time in liquid-penetrant inspection. Part 1. Material factors” in which the material factors influencing essentially the development time in penetrant testing have been marked out. These are: type of material, surface roughness and imperfection width. In the paper it has been described how to prepare the factorial plan which will enable to test every factor with taking into account its different values. Moreover, it has been presented investigations on natural cracks, their width and roughness profile what will allow to assign suitable values of independent variables to the factorial plan. The purpose of the plan prepared in such a way will be the determination of the influence of the material kind, surface roughness and discontinuity width on the development time in penetrant testing.
Źródło:
Archives of Metallurgy and Materials; 2016, 61, 3; 1697-1708
1733-3490
Pojawia się w:
Archives of Metallurgy and Materials
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Experimental Determination of Limit Adhesive Shear Stress between Solid Wall and Liquid
Autorzy:
Sawicki, J. M.
Powiązania:
https://bibliotekanauki.pl/articles/241430.pdf
Data publikacji:
2014
Wydawca:
Polska Akademia Nauk. Instytut Budownictwa Wodnego PAN
Tematy:
hydromechanics
slip boundary conditions
adhesion
Opis:
The slip boundary condition can be a very useful relation when some specific problems of hydromechanics are considered. According to the classical form of this condition, the slip of a fluid with respect to the solid wall should occur even at a very low velocity of motion. However, both theoretical analysis and certain empirical data suggest that there must be a limit value of the wall shear stress, below which the slip does not occur. According to the simplified balance of adhesive and gravitational forces, a simple experimental method for determining this stress has been proposed and applied in this paper.
Źródło:
Archives of Hydro-Engineering and Environmental Mechanics; 2014, 61, 3-4; 175--181
1231-3726
Pojawia się w:
Archives of Hydro-Engineering and Environmental Mechanics
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Material factors in relation to development time in liquid-penetrant inspection. Part 3. Testing of model plates
Autorzy:
Irek, P.
Słania, J.
Powiązania:
https://bibliotekanauki.pl/articles/356491.pdf
Data publikacji:
2017
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
imperfection
defect
penetration testing
adhesion
Opis:
The paper is the continuation of the previous ones entitled „Material factors in relation to development time in liquid-penetrant inspection. Part 1. Material factors“ and „Material factors in relation to development time in liquid-penetrant inspection. Part 2. Investigation programme and preliminary tests“ in which material factors influencing essentially the development time in penetrant testing as well as the range of their values have been specified. These factors are: material kind, surface roughness and imperfection width. In the paper it has been described how far the development time in colour penetrant testing is influenced by an individual material factor. Moreover, it has been presented the equation being the function of material factors and development time.
Źródło:
Archives of Metallurgy and Materials; 2017, 62, 1; 41-49
1733-3490
Pojawia się w:
Archives of Metallurgy and Materials
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
The chaperone-usher pathway of bacterial adhesin biogenesis - from molecular mechanism to strategies of anti-bacterial prevention and modern vaccine design*
Autorzy:
Piątek, Rafał
Zalewska, Beata
Bury, Katarzyna
Kur, Józef
Powiązania:
https://bibliotekanauki.pl/articles/1041367.pdf
Data publikacji:
2005
Wydawca:
Polskie Towarzystwo Biochemiczne
Tematy:
chaperone-usher pathway
pili
adhesion
fimbriae
Opis:
The chaperone-usher system determines the biogenesis of surface-exposed adhesive structures responsible for virulence of many Gram-negative bacteria. Investigations of the last 20 years have resolved the mechanism of this pathway on a structural level for different species of pathogenic bacteria. The purpose of this review is to present the molecular mechanisms of the biogenesis of adhesive structures assembled via the chaperone-usher pathway. The obtained mechanistic data allow one to propose potential strategies of anti-bacterial action. Additionally, the specific properties of the polymeric adhesive structures (pili and fimbriae) of the chaperone-usher system allow their use as effective and safe recombinant vaccines carrying foreign epitopes in thousands of copies on bacterial cell surface.
Źródło:
Acta Biochimica Polonica; 2005, 52, 3; 639-646
0001-527X
Pojawia się w:
Acta Biochimica Polonica
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Health-promoting properties exhibited by Lactobacillus helveticus strains
Autorzy:
Skrzypczak, Katarzyna
Gustaw, Waldemar
Waśko, Adam
Powiązania:
https://bibliotekanauki.pl/articles/1038894.pdf
Data publikacji:
2015
Wydawca:
Polskie Towarzystwo Biochemiczne
Tematy:
Lactobacillus helveticus
probiotics
adhesion
autoaggregation
coaggregation
Opis:
Many strains belonging to lactobacilli exert a variety of beneficial health effects in humans and some of the bacteria are regarded as probiotic microorganisms. Adherence and capabilities of colonization by Lactobacillus strains of the intestinal tract is a prerequisite for probiotic strains to exhibit desired functional properties. The analysis conducted here aimed at screening strains of Lactobacillus helveticus possessing a health-promoting potential. The molecular analysis performed, revealed the presence of a slpA gene encoding the surface S-layer protein SlpA (contributing to the immunostimulatory activity of L. helveticus M 92 probiotic strain) in all B734, DSM, T80, and T105 strains. The product of gene amplification was also identified in a Bifidobacterium animalis ssp. lactis BB12 probiotic strain. SDS-PAGE of a surface protein extract demonstrated the presence of a protein with a mass of about 50 kDa in all strains, which refers to the mass of the S-layer proteins. These results are confirmed by observations carried with transmission electron microscopy, where a clearly visible S-layer was registered in all the strains analyzed. The in vitro study results obtained indicate that the strongest adhesion capacity to epithelial cells (HT-29) was demonstrated by L. helveticus B734, while coaggregation with pathogens was highly diverse among the tested strains. The percentage degree of coaggregation was increasing with the incubation time. After 5 h of incubation, the strongest ability to coaggregate with Escherichia coli was expressed by T104. The T80 strain demonstrated a significant ability to co-aggregate with Staphylococcus aureus, while DSM with Bacillus subtilis. For B734, the highest values of co-aggregation coefficient was noted in samples with Salmonella. The capability of autoaggregation, antibiotic susceptibility, resistance to increasing salt concentrations, and strain survival in simulated small intestinal juice were also analyzed.
Źródło:
Acta Biochimica Polonica; 2015, 62, 4; 713-720
0001-527X
Pojawia się w:
Acta Biochimica Polonica
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
contact problems with friction, adhesion and wear in orthopaedic biomechanics. Part I - general developments
Autorzy:
Rojek, J.
Telega, J. J.
Powiązania:
https://bibliotekanauki.pl/articles/279902.pdf
Data publikacji:
2001
Wydawca:
Polskie Towarzystwo Mechaniki Teoretycznej i Stosowanej
Tematy:
bone-implant interface
contact
adhesion
friction
Opis:
The present paper is a continuation of the contribution by Rojek and Telega (1999). An alternative adhesion law is used to the study of the bone-implant interface. Various problems related to the bone-implant interface are discussed.
Zagadnienia kontaktowe z tarciem, adhezją i zużyciem w biomachanice ortopedycznej. Część I - Rozważania ogólne. Niniejsza praca stanowi kontynuację wcześniejszej pracy autorów, por. Rojek i Telega (1999). Zastosowano alternatywny model adhezji dla opisu interfazy kość-implant. Przedyskutowano równierz szereg zagadnień związanych z tą interfazą.
Źródło:
Journal of Theoretical and Applied Mechanics; 2001, 39, 3; 655-677
1429-2955
Pojawia się w:
Journal of Theoretical and Applied Mechanics
Dostawca treści:
Biblioteka Nauki
Artykuł
Wyświetlanie 1-15 z 299

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