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    Tytuł:
    Reversion of argE3 ochre strain Escherichia coli AB1157 as a tool for studying the stationary-phase (adaptive) mutations.
    Autorzy:
    Nowosielska, Anetta
    Grzesiuk, Elżbieta
    Powiązania:
    https://bibliotekanauki.pl/articles/1044376.pdf
    Data publikacji:
    2000
    Wydawca:
    Polskie Towarzystwo Biochemiczne
    Tematy:
    mutY
    replica plating
    Arg+ revertants
    Escherichia coli
    adaptive mutations
    mutT
    Opis:
    Adaptive (starvation-associated) mutations occur in non-dividing cells and allow growth under the selective conditions imposed. We developed a new method for the determination of adaptive mutations in Escherichia coli. The system involves reversion to prototrophy of the argE3OC mutation and was tested on AB1157 strains mutated in the mutT and/or mutY genes. The bacteria that mutated adaptively grow into colonies on minimal medium plates devoid of arginine (starvation conditions) when incubated longer than 4 days. Using the replica plating method we solved the problem of discrimination between growth-dependent and adaptive argE3→Arg+ revertants. Phenotype analysis and susceptibility of the Arg+ revertants to a set of T4 phage mutants create an additional possibility to draw a distinction between these two types of Arg+ revertants.
    Źródło:
    Acta Biochimica Polonica; 2000, 47, 2; 459-467
    0001-527X
    Pojawia się w:
    Acta Biochimica Polonica
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Zoonotic bacteria in the vicinity of animal farms as a factor disturbing the human microbiome: a review
    Autorzy:
    Kozajda, Anna
    Miśkiewicz, Emilia
    Jeżak, Karolina
    Powiązania:
    https://bibliotekanauki.pl/articles/31341039.pdf
    Data publikacji:
    2024-05-20
    Wydawca:
    Instytut Medycyny Pracy im. prof. dra Jerzego Nofera w Łodzi
    Tematy:
    environmental exposure
    microbiome
    livestock
    antibiotic resistance microorganisms
    ARG
    farm vicinity
    Opis:
    This review is aimed at summarizing the current state of knowledge about the relationship between environmental exposure to the bioaerosol emitted by intensive livestock farming and changes in the microbiome of people living in livestock farm vicinity. The PubMed, Scopus and Web of Science databases were searched by crossing keywords from the following 3 groups: a) “livestock,” “animal farms,” “animal breeding”; b) “microbiome,” “resistome”; c) “livestock vicinity,” “farm vicinity,” “neighborhoods and health” in 2010–2022. Literature screening did not reveal any paper related to the full microbiome composition in the population studied. In the study, the authors included 7 papers (5 from the Netherlands, 1 from the USA, and 1 from China). The studies confirmed the carriage of Staphylococcus aureus, including methicillin-resistant S. aureus (MRSA), livestock-associated MRSA (LA-MRSA MC398) and multidrug-resistant S. aureus (MDRSA) in the nasal microbiome of adults and children living within 500–2000 m from a livestock farm. Clostridium difficile, including LA-ribotype RT078 carriage, was detected in the intestinal microbiome of adults living within 500–1000 m. Extended-spectrum β-lactamase (ESBL) producing Enterobacteriaceae were confirmed in the intestinal microbiome of adults living within 500–6200 m. Knowledge on the composition of the microflora of people living in livestock farm vicinity is insufficient to conclude about changes in the microbiome caused by the environmental emission of bioaerosol. The carriage prevalence of the LA-bacteria, including both strains with antimicrobial resistance and antimicrobial resistance genes, confirms the presence of zoonotic bacteria in the human microflora in populations without occupational contact with animals. It cannot be ruled out that zoonotic bacteria, as a component of the microbiome, have a negative impact on people’s health.
    Źródło:
    International Journal of Occupational Medicine and Environmental Health; 2024, 37, 2; 138-152
    1232-1087
    1896-494X
    Pojawia się w:
    International Journal of Occupational Medicine and Environmental Health
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Reversion of argE3 to Arg+ in Escherichia coli AB1157 -an informative bacterial system for mutation detection
    Autorzy:
    Sikora, Anna
    Grzesiuk, Elżbieta
    Powiązania:
    https://bibliotekanauki.pl/articles/1040308.pdf
    Data publikacji:
    2010
    Wydawca:
    Polskie Towarzystwo Biochemiczne
    Tematy:
    mutations
    Arg+ revertants
    Escherichia coli K12
    prototrophy
    T4 phages
    suppressor tRNA
    Opis:
    This review concerns reversion of the argE3 (ochre) nonsense mutation to prototrophy in E. coli AB1157 strain as an informative system for mutation detection. Strain AB1157 bears the argE3 (ochre), hisG4 (ochre) and thr-1 (amber) mutations, and the supE44 amber suppressor on its chromosome. The Arg+ phenotype can be restored by (i) any base substitution at the argE3 site that changes the nonsense UAA codon to any sense nucleotide triplet or to UAG recognized by the supE44 amber suppressor, or (ii) suppressor mutations enabling the reading of the UAA nonsense codon. The argE3 → Arg+ reversion-based system enables (i) determination of the spontaneous or induced mutation level; (ii) determination of base substitutions (suppressor analysis); (iii) examination of transcription-coupled repair (TCR) since targets for DNA damage are situated on the transcribed or coding strand of DNA; (iv) detection of mutations resulting from single stranded DNA damage. This review focuses on studies carried out since the early 1990s till now with the application of the AB1157-based mutation detection system. Recently, the system has been used to obtain new data on the processes of methyl methanesulfonate-induced mutagenesis and DNA repair in E. coli alkB- mutants.
    Źródło:
    Acta Biochimica Polonica; 2010, 57, 4; 479-485
    0001-527X
    Pojawia się w:
    Acta Biochimica Polonica
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Spontaneous mutagenesis in exponentially growing and stationary-phase, umuDC-proficient and -deficient, Escherichia coli dnaQ49.
    Autorzy:
    Nowosielska, Anetta
    Nieminuszczy, Jadwiga
    Grzesiuk, Elżbieta
    Powiązania:
    https://bibliotekanauki.pl/articles/1041546.pdf
    Data publikacji:
    2004
    Wydawca:
    Polskie Towarzystwo Biochemiczne
    Tematy:
    dnaQ49
    argE3→Arg+ revertants
    E. coli
    Pol V
    spontaneous mutagenesis
    stationary-phase mutations
    Opis:
    Spontaneous mutations arise not only in exponentially growing bacteria but also in non-dividing or slowly dividing stationary-phase cells. In the latter case mutations are called adaptive or stationary-phase mutations. High spontaneous mutability has been observed in temperature sensitive Escherichia coli dnaQ49 strain deficient in 3'→5' proofreading activity assured by the ε subunit of the main replicative polymerase, Pol III. The aim of this study was to evaluate the effects of the dnaQ49 mutation and deletion of the umuDC operon encoding polymerase V (Pol V) on spontaneous mutagenesis in growing and stationary-phase E. coli cells. Using the argE3OC →Arg+ reversion system in the AB1157 strain, we found that the level of growth-dependent and stationary-phase Arg+ revertants was significantly increased in the dnaQ49 mutant at the non-permissive temperature of 37°C. At this temperature, in contrast to cultures grown at 28°C, SOS functions were dramatically increased. Deletion of the umuDC operon in the dnaQ49 strain led to a 10-fold decrease in the level of Arg+ revertants in cultures grown at 37°C and only to a 2-fold decrease in cultures grown at 28°C. Furthermore, in stationary-phase cultures Pol V influenced spontaneous mutagenesis to a much lesser extent than in growing cultures. Our results indicate that the level of Pol III desintegration, dependent on the temperature of incubation, is more critical for spontaneous mutagenesis in stationary-phase dnaQ49 cells than the presence or absence of Pol V.
    Źródło:
    Acta Biochimica Polonica; 2004, 51, 3; 683-692
    0001-527X
    Pojawia się w:
    Acta Biochimica Polonica
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    CADASIL spowodowany stereotypową mutacją p.Arg207Cys w genie NOTCH3
    CADASIL caused by stereotyped p.Arg207Cys mutation of NOTCH3 gene
    Autorzy:
    Jastrzębski, Karol
    Obrembska, Magdalena
    Kępczyński, Łukasz
    Turoboś, Katarzyna
    Sobczyńska-Tomaszewska, Agnieszka
    Miller, Elżbieta
    Głąbiński, Andrzej
    Powiązania:
    https://bibliotekanauki.pl/articles/1029714.pdf
    Data publikacji:
    2018
    Wydawca:
    Medical Communications
    Tematy:
    CADASIL
    NOTCH3
    choroba małych naczyń
    mózgowa autosomalna dominująca arteriopatia z podkorowymi zawałami i leukoencefalopatią
    p.Arg207Cys
    udar
    Opis:
    The most common hereditary cerebral small vessel disease, associated with strokes and vascular dementia, is known as cerebral autosomal dominant arteriopathy with subcortical infarcts and leukoencephalopathy (CADASIL). CADASIL is caused by mutations of the NOTCH3 gene. Since most pathogenic mutations at the protein level result with cysteine being replaced with another amino acid or another amino acid being replaced with cysteine, they are referred to as stereotyped mutations. A 55-year-old male patient, suddenly affected by speech disturbances, was diagnosed with a sporadic case of CADASIL on the basis of radiological imaging, particularly an magnetic resonance imaging scan. The diagnosis was conclusively confirmed by genetic testing, which revealed one of the rarer mutations, located in one allele of the NOTCH3 gene, namely p.Arg207Cys, reflecting at the DNA level a transition changing cytosine to thymine in position 619. In magnetic resonance imaging, classical radiological changes were seen, along with the presence of microhaemorrhages in subcortical nuclei, which is an atypical clinical manifestation of the disease. Despite the advanced cerebral changes, the patient continued to be professionally active. Currently, no effective treatment for the condition is available.
    Najczęstszą genetycznie uwarunkowaną chorobą małych naczyń związaną z udarami i naczyniopochodnym otępieniem jest mózgowa autosomalna dominująca arteriopatia z podkorowymi zawałami i leukoencefalopatią (cerebral autosomal dominant arteriopathy with subcortical infarcts and leukoencephalopathy, CADASIL). Za CADASIL odpowiedzialne są mutacje genu NOTCH3. Większość patogennych mutacji na poziomie białka skutkuje zastąpieniem cysteiny innym aminokwasem bądź innego aminokwasu cysteiną, dlatego mutacje te nazywa się stereotypowymi. U 55-letniego pacjenta, u którego nagle wystąpiły zaburzenia mowy, zdiagnozowano sporadyczny przypadek CADASIL-u. Podstawą podejrzenia choroby był obraz radiologiczny, a zwłaszcza obraz uzyskany w badaniu rezonansem magnetycznym. Badanie genetyczne potwierdziło diagnozę. Ujawniono jedną z rzadszych mutacji – w jednym allelu genu NOTCH3 p.Arg207Cys, odpowiadającą na poziomie DNA tranzycji cytozyny w tyminę w pozycji 619. W badaniu rezonansem magnetycznym odnotowano klasyczne zmiany radiologiczne i obecność mikrokrwawień w jądrach podkorowych, co nie jest częstą manifestacją choroby. Mimo tak zaawansowanych zmian pacjent kontynuował pracę zawodową. W chwili obecnej nie jest znane skuteczne leczenie.
    Źródło:
    Aktualności Neurologiczne; 2018, 18, 1; 47-51
    1641-9227
    2451-0696
    Pojawia się w:
    Aktualności Neurologiczne
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
      Wyświetlanie 1-5 z 5

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