Temat: 6-benzylaminopurine - Katalog OPAC zbiorów

Skocz do pozycji: 1.

Tytuł:: High-frequency adventitious shoot organogenesis from in vitro stem explants of Scutellaria araxensis Grossh
Autorzy:: Gharari, Zahra
Bagheri, Khadijeh
Sharari, Ali
Powiązania:: https://bibliotekanauki.pl/articles/16648097.pdf
Data publikacji:: 2022
Wydawca:: Polska Akademia Nauk. Czasopisma i Monografie PAN
Tematy:: 6-benzylaminopurine
direct organogenesis
Scutellaria araxensis
thidiazuron
Opis:: The present study introduced an in vitro shoot organogenesis protocol for the medicinal plant Scutellaria araxensis (Lamiaceae). Stem, leaf, and petiole explants were cultured in half-strength Murashige and Skoog (MS) medium containing different concentrations of 6-benzylaminopurine (BAP) alone or in combination with thidiazuron (TDZ), indole-3-butyric acid (IBA), or α-naphthalene acetic acid. Callus formation occurred from stem and petiole explants in most cultures; however, in leaf explants, it was observed only in cultures containing 0.5 mg/l BAP supplemented with TDZ at all concentrations. The highest frequency of indirect shoot induction (100 and 90%) with an average of 20.33 and 12 shoots per explant was observed in stem-derived calli cultured on halfstrength MS medium containing 2.0 mg/l BAP plus 0.5 and 1.5 mg/l TDZ, respectively. The best direct shoot organogenesis (40%) was observed in stem explants cultured on half-strength MS medium containing 0.5 mg/l BAP and 0.5 mg/l IBA with a mean of 18 shoots per stem explant. The regenerated micro-shoots were elongated on a medium fortified with 0.5 mg/l gibberellic acid and then successfully rooted in half-strength MS medium supplemented with 0.5 mg/l IBA. The obtained plantlets were acclimatized in a growth chamber with a survival rate of 100%. This study is the first report of a simple and efficient in vitro shoot organogenesis and regeneration protocol for S. araxensis by using stem explants, which could be useful for the conservation, genetic manipulation, and exploitation of biological molecules of this valuable genetic source.
Źródło:: BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology; 2022, 103, 2; 143-151
0860-7796
Pojawia się w:: BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology
Dostawca treści:: Biblioteka Nauki

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Skocz do pozycji: 2.

Tytuł:: The effect of 6-benzylaminopurine, thidiazuron and the type of explants on in vitro propagation of Yucca elephantipes Regel
Wpływ 6-benzyloaminopuryny, tidiazuronu i rodzaju eksplantatu na rozmnażanie Yucca elephantipes Regel in vitro
Autorzy:: Kozak, D.
Powiązania:: https://bibliotekanauki.pl/articles/11541939.pdf
Data publikacji:: 2010
Wydawca:: Uniwersytet Przyrodniczy w Lublinie. Wydawnictwo Uniwersytetu Przyrodniczego w Lublinie
Tematy:: 6-benzylaminopurine
thidiazuron
explant type
in vitro propagation
Yucca elephantipes
propagation
ornamental plant
Agavaceae
Opis:: Yucca elephantipes is an important commercial ornamental pot plant, excellent for growing in flats, patios or winter gardens. Traditional vegetative propagation of the most decorative yuccas is complicated due to a very low rate of propagation, so in vitro culture is an alternative method for commercial propagation of these plants. The influence of BA (0.4, 2.2, 4.4, 11.1, 22.2 µM) and TDZ (0.5, 2.3, 4.5, 11.4, 22.7 µM) on shoot multiplication of Yucca elephantipes Regel on Murashige and Skoog (MS) medium was studied. Explants cultured on medium without growth substances were used as a control. The two types of explants used in the experiment: shoot tips and nodal segments of shoots, were obtained from aseptically grown shoot clusters. When comparing regeneration capability of 2 types of Yucca elephantipes explants, it was found that more newly formed shoots and roots were obtained from nodes. The highest formation of shoots was obtained from nodes on MS medium supplemented with 4.5 µM TDZ or 11.1 and 22.2 µM BA (6.5, 6.0, 5.8, respectively). The shoots regenerated from nodes showed best elongation. On the control medium and on the media with the lowest level of BA or TDZ, their average length was 31.0–37.8 mm. The growth regulator-free medium and the media with a low level of BA were the most effective in inducing roots.
Juka słoniostopa (Yucca elephantipes) jest cenną rośliną pojemnikową do mieszkań, patio, a także do ogrodów zimowych. Tradycyjna metoda rozmnażania wegetatywnego większości gatunków ozdobnych juki jest mało wydajna, stąd zastosowanie metody kultur in vitro stwarza duże możliwości zwiększenia współczynnika mnożenia przy produkcji tych roślin na skalę masową. Badano wpływ BA (0,4; 2,2; 4,4; 11,1; 22,2 µM) i TDZ (0,5; 2,3; 4,5; 11,4; 22,7 µM) na namnażanie pędów Yucca elephantipes Regel na pożywce Murashige’a i Skooga (MS). Eksplantaty rosnące na pożywce bez regulatorów wzrostu stanowiły kontrolę. Pobrano dwa rodzaje eksplantatów: wierzchołki pędów i odcinki węzłowe, które pochodziły z kultur sterylnych. Porównując zdolności regeneracyjne eksplantatów Yucca elephantipes, stwierdzono, że więcej nowych pędów i korzeni regenerowało z odcinków węzłowych. Najlepszą regenerację pędów uzyskano z odcinków węzłowych na pożywkach z dodatkiem 4,5 µM TDZ lub 11,1 i 22,2 µM BA (6,5; 6,0; 5,8, odpowiednio). Pędy uzyskane z węzłów wykazywały lepszy wzrost elongacyjny. Na pożywce kontrolnej oraz w obecności niższych stężeń BA lub TDZ ich średnia długość wynosiła 31,0-37,8 mm. Najsilniejszą indukcję korzeni obserwowano na pożywce kontrolnej oraz przy dodatku BA w niskim stężeniu.
Źródło:: Acta Scientiarum Polonorum. Hortorum Cultus; 2010, 09, 3; 211-219
1644-0692
Pojawia się w:: Acta Scientiarum Polonorum. Hortorum Cultus
Dostawca treści:: Biblioteka Nauki

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Skocz do pozycji: 3.

Tytuł:: Auxin and cytokinin synergism in micropropagation for mass production of Aloe vera
Autorzy:: Yasmin, Sabina
Hasan, Jakir
Hossain, Shakhaowat
Saha, Sumitra
Khatun, Fahmida
Powiązania:: https://bibliotekanauki.pl/articles/16648118.pdf
Data publikacji:: 2022
Wydawca:: Polska Akademia Nauk. Czasopisma i Monografie PAN
Tematy:: plant growth regulators
6-benzylaminopurine
thidiazuron
naphthaleneacetic acid
indole-3-butyric acid
in vitro propagation
Opis:: Aloe vera [Aloe vera (L.) Burm. f.] is considered a valuable medicinal plant worldwide due to its remarkable beneficial effects on human health. However, challenges in A. vera propagation hinder meeting the increasing demand in the health and beauty sectors. As an alternative method, in vitro propagation is crucial for the mass production of Aloe plants, which is a rapid method as well. Therefore, the present study aimed to establish an efficient micropropagation protocol for A. vera by in vitro optimization of the effect of different plant growth regulators (PGRs). For shoot proliferation, sterilized explants were inoculated on the Murashige and Skoog (MS) medium supplemented with 6-benzylaminopurine (BAP) and thidiazuron (0.5, 1.0, 2.0, and 4.0 mg/l) in combination with 0.5 mg/l naphthaleneacetic acid (NAA). Subsequently, indole-3-butyric acid (IBA) (1.0, 2.0, and 3.0 mg/l) was used for root induction. It was found that the explants cultured on the MS medium supplemented with 4.0 mg/l BAP + 0.5 mg/l NAA showed the highest percentage of response (90 ± 1.29) for shoot induction within the minimum number of days (5 ± 0.33). The highest number of shoots (2.7 ± 0.36) and length of shoots (4.7 ± 0.42 cm) per explant were also observed with the same concentration of PGRs. However, the highest number of roots (3.2 ± 0.57), length of roots (5.67 ± 0.21 cm), and root induction (80 ± 1.97 %) were noticed within the minimum number of days (11 ± 0.79) on the MS medium supplemented with 1.0 mg/l IBA. Thus, the proposed method is a quick and effective approach for the mass propagation of A. vera with appropriate dosages of auxins and cytokinins, which may allow meeting the increasing commercial demand.
Źródło:: BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology; 2022, 103, 3; 301-310
0860-7796
Pojawia się w:: BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology
Dostawca treści:: Biblioteka Nauki