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    Wyświetlanie 1-6 z 6
    Tytuł:
    A Hotspot of Amoebae Diversity: 8 New Naked Amoebae Associated with the Planktonic Bloom-forming Cyanobacterium Microcystis
    Autorzy:
    van Wichelen, Jeroen
    Bass, David
    Berney, Cédric
    Claeys, Myriam
    d’Hondt, Sofie
    Vanormelingen, Pieter
    Vyverman, Wim
    Powiązania:
    https://bibliotekanauki.pl/articles/763601.pdf
    Data publikacji:
    2016
    Wydawca:
    Uniwersytet Jagielloński. Wydawnictwo Uniwersytetu Jagiellońskiego
    Tematy:
    Naked amoebae, Microcystis, morphology, 18S rDNA, harmful algal blooms, grazing, Europe
    Opis:
    The colonies of Microcystis, one of the most common bloom-forming cyanobacteria worldwide, harbor a diverse community of microorganisms. Among these, naked amoebae feeding on Microcystis cells can strongly influence natural Microcystis population dynamics. In this study, we investigated the species diversity of these amoebae based on 26 Microcystis-associated amoebae (MAA) strains from eutrophied water bodies in Belgium and elsewhere in western Europe. A detailed morphological characterization in combination with 18S rDNA (SSU) phylogenies revealed the presence of no less than 10 species. Nine of these belonged to the known genera Vannella (2 species), Korotnevella (2), Copromyxa (2), Vexillifera (1), Cochliopodium (1) and the recently described Angulamoeba (1). Only two were previously described, the others were new to science. One taxon could not be assigned to a known genus and is here described as Schoutedamoeba gen. n., representing a new variosean lineage. The discovery of so many new species from only one very specific habitat (Microcystis colonies) from a rather restricted geographical area indicates that the diversity of planktonic naked amoebae is much higher than previously appreciated and that only a tiny fraction of the total diversity of naked amoebae is currently known.
    Źródło:
    Acta Protozoologica; 2016, 55, 2
    1689-0027
    Pojawia się w:
    Acta Protozoologica
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Two new Myxobolus spp. (Myxozoa: Myxobolidae) from white bream, Blicca bjoerkna (Linnaeus, 1758) developing in basifilamental location of gills
    Autorzy:
    Molnár, Kálmán
    Eszterbauer, Edit
    Guti, Csaba F.
    Székely, Csaba
    Powiązania:
    https://bibliotekanauki.pl/articles/763533.pdf
    Data publikacji:
    2014
    Wydawca:
    Uniwersytet Jagielloński. Wydawnictwo Uniwersytetu Jagiellońskiego
    Tematy:
    Myxozoa, site selection, tissue tropism, occurrence, histology, 18S rDNA, phylogeny
    Opis:
    Two new Myxobolus species, M. bjoerknae sp. n. and M. lamellobasis sp. n. have been described from the gills of white bream, Blicca bjoerkna. Plasmodia of M. bjoerknae sp. n. developed in the connective tissue inside the cartilaginous gill arch, while plasmodia of M. lamellobasis sp. n. seem to start their development in the multilayered epithelium between two lamellae close to the base of gill filaments. Then they may bulge out of the interlamellar space fused to a large bulk locating at the base of filaments. The large, ellipsoidal spores of M. bjoerknae sp. n. 17.4 × 13.1 µm in size, resembled the spores of other species developing in the gill arch (e.g. M. fundamentalis, M. gayerae, and M. pfeifferi), but differed from them in its 18S rDNA sequence. Roundish spores of M. lamellobasis sp. n. with a size of 11.1 × 8.6 µm resembled the spores of M. impressus developing interlamellarly and the spores of M. rotundus, M. parviformis, and M. muellericus having intralamellar localization. However, the detected genetic difference clearly distinguished it from the other species developing in similar tissue location. The phylogenetic location of the two newly described species seems to correlate both with spore shape and fish host species.
    Źródło:
    Acta Protozoologica; 2014, 53, 3
    1689-0027
    Pojawia się w:
    Acta Protozoologica
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Occurrence of Ditylenchus destructor Thorne, 1945 on a sand dune of the Baltic Sea
    Autorzy:
    Dobosz, R.
    Rybaczyk-Mydlowska, K.
    Winiszewska, G.
    Powiązania:
    https://bibliotekanauki.pl/articles/2084759.pdf
    Data publikacji:
    2020
    Wydawca:
    Polska Akademia Nauk. Czytelnia Czasopism PAN
    Tematy:
    Ammophila arenaria
    internal transcribed spacer (ITS)
    potato rot nematode
    18S
    28S rDNA
    Opis:
    Ditylenchus destructor is a serious pest of numerous economically important plants worldwide. The population of this nematode species was isolated from the root zone of Ammophila arenaria on a Baltic Sea sand dune. This population’s morphological and morphometrical characteristics corresponded to D. destructor data provided so far, except for the stylet knobs’ height (2.1–2.9 vs 1.3–1.8) and their arrangement (laterally vs slightly posteriorly sloping), the length of a hyaline part on the tail end (0.8–1.8 vs 1–2.9), the pharyngeal gland arrangement in relation to the intestine (dorsal or ventral vs dorsal, ventral or lateral) and the appearance of vulval lips (smooth vs annulated). Ribosomal DNA sequence analysis confirmed the identity of D. destructor from a coastal dune.
    Źródło:
    Journal of Plant Protection Research; 2020, 60, 1; 31-40
    1427-4345
    Pojawia się w:
    Journal of Plant Protection Research
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Molecular Phylogenetics Evidence for a Novel Lineage of Amoebae Within Discosea (Amoebozoa: Lobosa)
    Autorzy:
    Corsaro, Daniele
    Venditti, Danielle
    Powiązania:
    https://bibliotekanauki.pl/articles/763405.pdf
    Data publikacji:
    2013
    Wydawca:
    Uniwersytet Jagielloński. Wydawnictwo Uniwersytetu Jagiellońskiego
    Tematy:
    Amoebozoa, environmental 18S rDNA, small amoebae, Dermamoebida
    Opis:
    Some amoebae were recovered from freshwater samples on agar plates. Due to a fungal contamination tightly associated with these amoebae, it was impossible to correctly characterize them on a morphological base, but sequences of the small subunit ribosomal RNA gene (SSU rDNA) were successfully obtained from three strains. Phylogenetic analysis performed on these SSU rDNA allowed to identify these amoebae as members of a new lineage, related to the Dermamoebida, which includes also several other environmental SSU sequences.
    Źródło:
    Acta Protozoologica; 2013, 52, 4
    1689-0027
    Pojawia się w:
    Acta Protozoologica
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Wpływ metodyki badań na ocenę struktury zbiorowisk mikroorganizmów w glebie leśnej
    Effect of the methodology of studies on the structure of the microorganisms communities in the forest soil
    Autorzy:
    Behnke-Borowczyk, J.
    Kwaśna, H.
    Powiązania:
    https://bibliotekanauki.pl/articles/989286.pdf
    Data publikacji:
    2016
    Wydawca:
    Polskie Towarzystwo Leśne
    Tematy:
    lesnictwo
    gleby lesne
    mikroorganizmy glebowe
    grzyby mikroskopowe
    struktura zbiorowisk
    metodyka badan
    metody klasyczne
    metody molekularne
    startery NS1
    startery NS2
    DNA
    region 18S
    classical method of isolation
    fungi
    microorganisms
    ns1
    ns2
    18s rdna
    soil
    Opis:
    Two different communities of microorganisms were identified in soils by application of the classical method of fungi isolation (soil dilution, culturing on artificial media, morphotyping) and a molecular method (extraction of the environmental DNA, amplification with universal primers NS1 and NS2, cloning and sequencing of representative clones). No organisms were common to both communities. Apart from rare representatives of the Animalia, communities included single fungus−like Eucarya belonging to the Protista, Class Oomycota, and numerous fungi belonging to Chytridiomycota, Zygomycota, Ascomycota and Basidiomycota orders. In total, 88 species were identified in four soil samples. Fungi were mostly Ascomycota. The classical method was particularly effective in detection of fungi important for creation of phytosanitary conditions of soil, i.e. antagonists (Penicillium, Tolypocladium and Trichoderma) and potential stimulants (dark−pigmented Hormiactis candida, Humicola spp. and Phialophora spp.) of phytopathogens (including the common forest genera Armillaria and Heterobasidion). Application of the classical method allowed the detection of mycorrhizal Ascomycota from the genus Oidiodendron. Application of the molecular method allowed the detection of 13 mycorrhizal Basidiomycota. Although primers NS1 and NS2 were designed from a match with DNA of culturable organisms, they also amplified the DNA of non−culturable organisms. This emphasizes their potential usefulness in studies of the biodiversity of microorganisms in environmental samples. The shortage of reference sequences in the database discourages use of the 18S rDNA region in studies on fungal communities. The studies on the biodiversity of microorganisms need the application of a few independent methods of detection and identification.
    Źródło:
    Sylwan; 2016, 160, 06; 492-503
    0039-7660
    Pojawia się w:
    Sylwan
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Zastosowanie regionu ITS1/2 rDNA i 18S rDNA do badania mykobioty gleby leśnej
    Use of ITS1/2 rDNA and 18S rDNA in studies of the forest soil mycobiota
    Autorzy:
    Behnke-Borowczyk, J.
    Kwaśna, H.
    Powiązania:
    https://bibliotekanauki.pl/articles/989532.pdf
    Data publikacji:
    2016
    Wydawca:
    Polskie Towarzystwo Leśne
    Tematy:
    lesnictwo
    gleby lesne
    mikroorganizmy glebowe
    grzyby glebowe
    struktura zbiorowisk
    metody badan
    metody molekularne
    DNA rybosomalny
    region ITS1/2
    region 18S
    detection
    forest
    its1/2 rdna
    ns1
    ns2
    18s rdna
    microorganisms
    mycobiota
    soil
    Opis:
    The aim of the studies was to check the usefulness of ITS1/2 rDNA and 18S rDNA regions in the molecular investigation of forest soil microbiota structure. Soil studied, originated from a 1−year−old plantation and a 40−year old stand of Scots pine located in Bierzwnik and Międzychów forest districts located 200 km apart. The hypothesis assumed that both approaches lead to the discovery of abundant microbiota communities with different structures and with rare common species. The environmental DNA was extracted with a Power Soil ® DNA Isolation Kit from two soil samples in each site. The ITS1/2 rDNA was amplified with specific primers ITS1 and ewfitsrev 1, and 18S rDNA with universal primers NS1 and NS2. PCR products were cloned into pGEM−T Easy. Inserts were primarily selected in blue/white screening on a X−gal medium. Representative clones were further selected in two separate RFLP analyses with HhaI and BsuRI restriction enzymes. Representative clones purified and sequenced using the Sanger Method in the DNA Research Centre (Poznań). Each sequence was identified to the lowest taxonomic rank. Ninety to 233 clones with DNA of 5−44 taxa including 3−37 taxa of fungi were obtained from 4 samples of soil. After application of ITS1/2 rDNA and 18S rDNA, the fungal DNA was detected respectively in 89,60−100,00% and 11,77−64,8% clones and the number of fungal species detected was respectively 12−37 and 3−19. Fungi were represented by four orders: Chytridiomycota, Zygomycota, Ascomycota and Basidiomycota. Both primers also amplified also DNA of other organisms (mostly from Animalia and Protista Kingdom) represented by 0−9 taxa. If compared, the application of forest soil microbiota structure with ITS1/2 rDNA and 18S rDNA led to detect a lower abundance of fungi and a bigger abundance of other organisms. Considering the higher number of clones and taxa recognized, the region of ITS1/2 rDNA was more effective in the studies of the soil microbiota structure. The region of 18S rDNA was efficient in local detection of Chytridiomycota and Zygomycota and of rare species of fungi from Ascomycota and Basidiomycota. Despite the deficiency of NCBI database the use of the 18S rDNA region in studies on fungal community the region should be included in molecular studies of fungal diversity. It is concluded that studies on the biodiversity of soil microorganisms need the application of a few independent methods of detection and identification.
    Źródło:
    Sylwan; 2016, 160, 07; 564-572
    0039-7660
    Pojawia się w:
    Sylwan
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
      Wyświetlanie 1-6 z 6

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