- Tytuł:
- Carbon isotope effects in the studies of the mechanism of action of tyrosine phenol-lyase
- Autorzy:
-
Augustyniak, W.
Kański, R.
Kańska, M. - Powiązania:
- https://bibliotekanauki.pl/articles/148527.pdf
- Data publikacji:
- 2006
- Wydawca:
- Instytut Chemii i Techniki Jądrowej
- Tematy:
-
12C/14C kinetic isotope effects
tyrosine phenol-lyase - Opis:
- 12C/14C kinetic isotope effects on the hydrolytic cleavage of tyrosine to phenol and ammonium pyruvate catalyzed by Citrobacter freundii tyrosine phenol-lyase have been determined in positions 2, 3 and ring-1' of L-tyrosine. The competitive method with dual-label approach was applied with 3',5'-ring 3H as remote label. The results revealed the change of the effect on carbon atom in position 2 during the reaction course from the high normal values to low inverse values. On the other hand, the effect values on 3 and ring-1' position remained constant during the reaction course. The discussion of these results regarding the reaction mechanism is presented.
- Źródło:
-
Nukleonika; 2006, 51,suppl.2; 7-11
0029-5922
1508-5791 - Pojawia się w:
- Nukleonika
- Dostawca treści:
- Biblioteka Nauki
Artykuł