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    Wyświetlanie 1-9 z 9
    Tytuł:
    Molecular cloning and expression analysis of CML27 in Brassica oleracea pollination process
    Autorzy:
    Lian, X.P.
    Zeng, J.
    Zhang, H.C.
    Yang, X.H.
    Zhao, L.
    Zhu, L.Q.
    Powiązania:
    https://bibliotekanauki.pl/articles/19583.pdf
    Data publikacji:
    2018
    Wydawca:
    Polska Akademia Nauk. Czytelnia Czasopism PAN
    Źródło:
    Acta Biologica Cracoviensia. Series Botanica; 2018, 60, 2
    0001-5296
    Pojawia się w:
    Acta Biologica Cracoviensia. Series Botanica
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Molecular cloning, bioinformation analysis and expression of the strictosidine synthase in Dendrobium officinale
    Autorzy:
    Zhu, Y.-F.
    Fan, H.-H.
    Li, D.-H.
    Jin, Q.
    Zhang, C.-M.
    Zhu, L.-Q.
    Song, C.
    Cai, Y.-P.
    Lin, Y.
    Powiązania:
    https://bibliotekanauki.pl/articles/12715947.pdf
    Data publikacji:
    2020
    Wydawca:
    Uniwersytet Przyrodniczy w Lublinie. Wydawnictwo Uniwersytetu Przyrodniczego w Lublinie
    Opis:
    The enzyme strictosidine synthase (STR, EC: 4.3.3.2) plays a key role in the biosynthetic pathway of terpenoid indole alkaloid (TIA). It catalyzes the condensation of the tryptamine and secologanin to form 3α(S)-strictosidine, which is the common precursor of all TIAs. In this paper, a STR gene designated as DoSTR (GenBank: KX068707) was first cloned and characterized from Dendrobium officinale with rapid amplified cDNA ends method (RACE). DoSTR has a length of 1380bp with 1179bp open reading frame encoding 392 amino acids. BlastP analyses showed that its amino acid sequence was classified into Str_synth superfamily. qRT-PCR showed that DoSTR was expressed in all tissues tested, with a significantly higher level in flower and the lowest in stem. Four different treatments with MeJA, SA, ABA and AgNO₃, respectively, could induce the DoSTR expression to a different extent. And the effect of MeJA was the most obvious and transcript level of DoSTR induced by MeJA was 20.7 times greater than that of control at 48 hours after treatment. Furthermore, it was found that DoSTR was localized in vacuole through transient expression in tobacco. The characterization and expression of DoSTR can help in further studying the role of DoSTR in the biosynthesis of TIAs in D. officinale. This study may throw light on the alkaloid biosynthesis pathway of D. officinale.
    Źródło:
    Acta Scientiarum Polonorum. Hortorum Cultus; 2020, 19, 3; 111-124
    1644-0692
    Pojawia się w:
    Acta Scientiarum Polonorum. Hortorum Cultus
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Near-field multiple optical trapping using high order axially symmetric polarized beams
    Autorzy:
    Zhou, Z
    Tan, Q
    Zhu, L
    Yang, C
    Powiązania:
    https://bibliotekanauki.pl/articles/174886.pdf
    Data publikacji:
    2013
    Wydawca:
    Politechnika Wrocławska. Oficyna Wydawnicza Politechniki Wrocławskiej
    Tematy:
    optical trapping
    surface plasmon polaritons
    axially symmetric polarized beams
    Opis:
    The near-field multiple optical trapping using high order axially symmetric polarized beams (ASPBs) is studied for the first time. First, a near-field optical trapping scheme is proposed based on the Kretschmann–Raether configuration, and surface plasmon polaritons (SPPs) field distributions excited by incident ASPBs are calculated, which present a multi-focal-spot pattern and the size of spots is much smaller than that of the diffraction limitation. Then, the gradient forces on Rayleigh dielectric particles formed by the multi-focal-spot focused field are computed, which indicates that multiple ultra-small particles with the refractive index higher than that of the ambient medium can be trapped simultaneously on the metal surface. The number and size of trapped particles can be manipulated by flexibly modifying the polarization order of incident beams, which is expected to enhance the capability of traditional optical trapping systems and provide a solution for massively parallel optical trapping of nanometer-sized particles.
    Źródło:
    Optica Applicata; 2013, 43, 2; s. 287-296
    0078-5466
    1899-7015
    Pojawia się w:
    Optica Applicata
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Enhancing the Thermal Protective Performance of Firefighters Protective Fabrics by Incorporating Phase Change Materials
    Polepszenie właściwości termicznych odzieży ochronnej strażaków poprzez zastosowanie materiałów zmiennofazowych
    Autorzy:
    Zhu, F L
    Feng, Q Q
    Liu, R
    Yu, B
    Zhou, Y
    Powiązania:
    https://bibliotekanauki.pl/articles/231560.pdf
    Data publikacji:
    2015
    Wydawca:
    Sieć Badawcza Łukasiewicz - Instytut Biopolimerów i Włókien Chemicznych
    Tematy:
    phase change material
    fire protective clothing
    thermal protective performance
    materiał zmiennofazowy
    odzież ochronna
    ochrona termiczna odzieży
    Opis:
    A composite fabric consisting of an outer shell, moisture barrier, thermal liner and comfort layer used for firefighters’ protective clothing, was incorporated with shape-stabilised phase change material (PCM) powder in order to improve the thermal protection capability provided by thermal protective clothing. Then we conducted a series of FTP (fire testing protection) experiments to investigate the effects of PCM location and phase change temperature on the heat protection efficiency of firefighters’ protective fabrics (FFPFs). Simultaneously the thermoregulation performance of the composite fabrics with PCMs was evaluated by using step-cooling experimental technology during the cooling process. Data from the FTP tests were also compared with those from the existing enthalpy formulation model of heat transfer through FFPFs embedded with PCMs. It was concluded that the use of PCMs could improve the heat buffering capacity. However, as PCM has a moderate melting temperature, it is better than the other two samples because they have a lower and higher melting temperature. Therefore PX 52 PCMs (melting temperature: 47 - 53 °C) could provided the maximum heat protection time compared with the other two kinds of PCMs.
    W celu zwiększenia zdolności ochrony termicznej odzieży ochronnej dla strażaków, przy użyciu stabilizowanego materiału zmiennofazowego w postaci proszku, stworzono materiał kompozytowy składający się z powłoki zewnętrznej, warstwy stanowiącej barierę dla wilgoci, wkładki termicznej i warstwy zapewniającej komfort. W celu zbadania wpływu umieszczenia materiału zmiennofazowego i temperatury przemiany fazowej na efektywność ochrony cieplnej przeprowadzono serię eksperymentów (testy ochrony przeciwpożarowej). Jednocześnie oceniono zdolność termoregulacji tkanin kompozytowych, oceny tej dokonano stosując technikę stopniowego chłodzenia. Dane z badań porównano z danymi teoretycznymi. Stwierdzono, że stosowanie materiałów zmiennofazowych może poprawić zdolność buforowania ciepła. Określono optymalny rodzaj materiału zmiennofazowego.
    Źródło:
    Fibres & Textiles in Eastern Europe; 2015, 2 (110); 68-73
    1230-3666
    2300-7354
    Pojawia się w:
    Fibres & Textiles in Eastern Europe
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Personal identification based on brain networks of EEG signals
    Autorzy:
    Kong, W.
    Jiang, B.
    Fan, Q.
    Zhu, L.
    Wei, X.
    Powiązania:
    https://bibliotekanauki.pl/articles/329856.pdf
    Data publikacji:
    2018
    Wydawca:
    Uniwersytet Zielonogórski. Oficyna Wydawnicza
    Tematy:
    electroencephalogram signal
    personal identification
    brain network
    phase synchronization
    elektroencefalogram
    identyfikacja osobowa
    sieć mózgowa
    synchronizacja fazy
    Opis:
    Personal identification is particularly important in information security. There are numerous advantages of using electroencephalogram (EEG) signals for personal identification, such as uniqueness and anti-deceptiveness. Currently, many researchers focus on single-dataset personal identification, instead of the cross-dataset. In this paper, we propose a method for cross-dataset personal identification based on a brain network of EEG signals. First, brain functional networks are constructed from the phase synchronization values between EEG channels. Then, some attributes of the brain networks including the degree of a node, the clustering coefficient and global efficiency are computed to form a new feature vector. Lastly, we utilize linear discriminant analysis (LDA) to classify the extracted features for personal identification. The performance of the method is quantitatively evaluated on four datasets involving different cognitive tasks: (i) a four-class motor imagery task dataset in BCI Competition IV (2008), (ii) a two-class motor imagery dataset in the BNCI Horizon 2020 project, (iii) a neuromarketing dataset recorded by our laboratory, (iv) a fatigue driving dataset recorded by our laboratory. Empirical results of this paper show that the average identification accuracy of each data set was higher than 0.95 and the best one achieved was 0.99, indicating a promising application in personal identification.
    Źródło:
    International Journal of Applied Mathematics and Computer Science; 2018, 28, 4; 745-757
    1641-876X
    2083-8492
    Pojawia się w:
    International Journal of Applied Mathematics and Computer Science
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Automatic Laser Interferometer And Vision Measurement System For Stripe Rod Calibration
    Autorzy:
    Zhao, M.
    Huang, Q.-H.
    Zhu, L.-J.
    Qiu, Z.-M.
    Powiązania:
    https://bibliotekanauki.pl/articles/221047.pdf
    Data publikacji:
    2015
    Wydawca:
    Polska Akademia Nauk. Czytelnia Czasopism PAN
    Tematy:
    stripe edge
    automatic control
    laser interferometer
    vision measurement
    Opis:
    In order to calibrate the stripe precision of a leveling rod, an automatic laser interferometer and a vision measurement system were designed by Xi’an University of Technology in China. The rod was driven by a closed-loop control and the data were collected at the stop state to ensure precision. The laser interferometer provided not only the long distance data but also a position feedback signal in the automatic control loop. CCD camera and a vision measurement method were used to inspect the stripe edge position and defect. Apixel-equivalent self-calibration method was designed to improve precision. ROI (regions of interest) method and an outline tracing method were designed to quickly extract multiple stripe edges. A combination of the image data with the interferometer data reduces control difficulty and ensures the measurement accuracy. The vision measurement method reached sub-pixel precision and the defective edges were reported. The system can automatically calibrate a stripe leveling rod with a high degree of efficiency and precision.
    Źródło:
    Metrology and Measurement Systems; 2015, 22, 4; 491-502
    0860-8229
    Pojawia się w:
    Metrology and Measurement Systems
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Differential expression of Toll - like receptor 4 signaling pathway genes in Escherichia coli F18 - resistant and - sensitive Meishan piglets
    Autorzy:
    Liu, Y.
    Gan, L.N.
    Qin, W.Y.
    Sun, S.Y.
    Zhu, G.Q.
    Wu, S.L.
    Bao, W.B.
    Powiązania:
    https://bibliotekanauki.pl/articles/31946.pdf
    Data publikacji:
    2016
    Wydawca:
    Polska Akademia Nauk. Czytelnia Czasopism PAN
    Opis:
    The Toll-like receptor 4 (TLR4) signaling pathway is an important inflammatory pathways associated with the progression of numerous diseases. The aim of the present study was to investigate the relationship between TLR4 signaling and resistance to Escherichia coli F18 in locally weaned Meishan piglets. Using a real-time PCR approach, expression profiles were determined for key TLR4 signaling pathway genes TLR4, MyD88, CD14, IFN-α, IL-1β and TNF-α in the spleen, thymus, lymph nodes, duodenum and jejunum of E. coli F18-resistant and -sensitive animals. TLR4 signaling pathway genes were expressed in all the immune organs and intestinal tissues, and the expression was generally higher in the spleen and lymph nodes. TLR4 transcription was higher in the spleen of sensitive piglets (p<0.05), but there was no significant difference in TLR4 mRNA levels in other tissues. Similarly, CD14 transcription was higher in lymph nodes of sensitive animals (p<0.05) but not in other tissues. IL-1β expression was higher in the spleen and in the duodenum of resistant piglets (p<0.05, p<0.01, respectively), and there were no significant differences in other tissues. There were also no significant differences in the expression of MyD88, TNF-α and IFN-α between sensitive and resistant piglets (p>0.05). These results further confirm the involvement of the TLR4 signaling pathway in resistance to E. coli F18 in Meishan weaned piglets. The resistance appeared to be mediated via downregulation of TLR4 and CD14, and upregulation of MyD88 that may promote the release of cytokines TNF-α, IL-1β, IFN-α and other inflammatory mediators which help to fight against E. coli F18 infection.
    Źródło:
    Polish Journal of Veterinary Sciences; 2016, 19, 2
    1505-1773
    Pojawia się w:
    Polish Journal of Veterinary Sciences
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Use of fluorescence quantitative polymerase chain reaction (PCR) for the detection of Escherichia coli adhesion to pig intestinal epithelial cells
    Autorzy:
    Dai, C.H.
    Gan, L.N.
    Qin, W.U.
    Zi, C.
    Zhu, G.Q.
    Wu, S.L.
    Bao, W.B.
    Powiązania:
    https://bibliotekanauki.pl/articles/31510.pdf
    Data publikacji:
    2016
    Wydawca:
    Polska Akademia Nauk. Czytelnia Czasopism PAN
    Opis:
    An efficient and accurate method to test Escherichia coli (E. coli) adhesion to intestinal epithelial cells will contribute to the study of bacterial pathogenesis and the function of genes that encode receptors related to adhesion. This study used the quantitative real-time polymerase chain reaction (qPCR) method. qPCR primers were designed from the PILIN gene of E. coli F18ab, F18ac, and K88ac, and the pig β-ACTIN gene. Total deoxyribonucleic acid (DNA) from E. coli and intestinal epithelial cells (IPEC-J2 cells) were used as templates for qPCR. The 2−ΔΔCt formula was used to calculate the relative number of bacteria in cultures of different areas. We found that the relative numbers of F18ab, F18ac, and K88ac that adhered to IPEC-J2 cells did not differ significantly in 6-, 12-, and 24-well culture plates. This finding indicated that there was no relationship between the relative adhesion number of E. coli and the area of cells, so the method of qPCR could accurately test the relative number of E. coli. This study provided a convenient and reliable testing method for experiments involving E. coli adhesion, and also provided innovative ideas for similar detection methods.
    Źródło:
    Polish Journal of Veterinary Sciences; 2016, 19, 3
    1505-1773
    Pojawia się w:
    Polish Journal of Veterinary Sciences
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Thermal Decomposition Kinetics of Hexanitrohexaazaisowurtzitane/Ammonium Perchlorate
    Autorzy:
    Zhu, Y.-L.
    Wang, K.-K.
    Shan, M.-X.
    Zheng, X.-D.
    Jiao, Q.-J.
    Wang, J.-S.
    Powiązania:
    https://bibliotekanauki.pl/articles/358067.pdf
    Data publikacji:
    2016
    Wydawca:
    Sieć Badawcza Łukasiewicz - Instytut Przemysłu Organicznego
    Tematy:
    hexanitrohexaazaisowurtzitane
    ammonium perchlorate
    thermal analysis
    thermal decomposition kinetics
    Opis:
    The thermal decomposition kinetics of hexanitrohexaazaisowurtzitane/ ammonium perchlorate (HNIW/AP) have been investigated by thermogravimetrydifferential scanning calorimetry-mass spectrometry (TG-DSC-MS) simultaneous analysis. TG showed that there were three weight loss processes for the thermal decomposition of the HNIW/AP mixture. The first was ascribed mainly to the thermal decomposition of HNIW, while the second and third were assigned to that of AP. The presence of AP has little effect on the thermal decomposition process of the HNIW component. The apparent activation energy of the thermal decomposition of the HNIW component, calculated by the Kissinger method, was little changed compared to that of neat HNIW. The addition of HNIW to AP caused the onset and end temperatures of the thermal decomposition to be decreased and the decomposition process to be shortened. The high-temperature and lowtemperature decomposition processes of AP became blurred in the presence of HNIW, and this was supported by the MS results.
    Źródło:
    Central European Journal of Energetic Materials; 2016, 13, 1; 149-159
    1733-7178
    Pojawia się w:
    Central European Journal of Energetic Materials
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
      Wyświetlanie 1-9 z 9

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