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Wyświetlanie 1-15 z 57
Tytuł:
Analyzing the bibliometric progress of sustainable monitoring and analysis of sports training loads
Autorzy:
Xing, Y.
Bibi, B.
Zhang, Y.M.
Mushtaq, R.T.
Powiązania:
https://bibliotekanauki.pl/articles/28762638.pdf
Data publikacji:
2023
Wydawca:
Instytut Medycyny Wsi
Źródło:
Annals of Agricultural and Environmental Medicine; 2023, 30, 2; 352-363
1232-1966
Pojawia się w:
Annals of Agricultural and Environmental Medicine
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Development of TaqMan-based real-time PCR assay based on the E1 genefor the quantitative detection of the Getah virus
Autorzy:
Lin, A.
Hu, X.
Cui, S.
Yang, T.
Zhang, Z.
Li, P.
Guo, M.
Lu, Y.
Powiązania:
https://bibliotekanauki.pl/articles/16647453.pdf
Data publikacji:
2023
Wydawca:
Polska Akademia Nauk. Czasopisma i Monografie PAN
Tematy:
Getah virus
real-time PCR
TaqMan
detection
Opis:
To develop a sensitive, specific, and rapid approach for the detection Getah virus (GETV), a set of primers targeting the conserved region of the E1 gene was created. The TaqMan-based real-time PCR method for GETV detection was developed by optimizing the reaction conditions. The method demonstrated excellent specificity, and amplification did not occur with the causative agents of all prevalent swine viral infections (CSFV, PRRSV, PRV, PEDV, PTV, and JEV), except GETV. Additionally, upon assessing the sensitivity of the method, the minimum detection limit for GETV was found to be 5.94 copies/μL, which is 10 times higher than that of the traditional PCR approach. Further, the intra- and inter-assay variation coefficients were less than 1%, demonstrating good repeatability. Moreover, GETV was found in 10 of the 20 field serum samples using real-time PCR but only in three of the samples using traditional PCR. Consequently, the first GETV TaqMan-based real-time PCR approach based on the E1 gene was developed for GETV pathogenic diagnoses, and this exhibited high specificity, sensitivity, and repeatability. This assay is practical for the pathogenic diagnosis and epidemiology of GETV.
Źródło:
Polish Journal of Veterinary Sciences; 2023, 26, 1; 21-28
1505-1773
Pojawia się w:
Polish Journal of Veterinary Sciences
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Rapid screening of monoclonal antibodies against porcine circovirus type 2 using colloidal gold-based paper test
Autorzy:
Jin, Q.Y.
Feng, L.L.
Wang, Y.B.
Li, P.
Yang, J.F.
Teng, M.
Chai, S.J.
Xing, G.X.
Zhang, G.P.
Powiązania:
https://bibliotekanauki.pl/articles/16539073.pdf
Data publikacji:
2022
Wydawca:
Polska Akademia Nauk. Czasopisma i Monografie PAN
Tematy:
porcine circovirus type 2 (PCV2)
screening of MAbs
paper test
Opis:
A proof of concept for using paper test as a suitable method in the production of monoclonal antibodies (MAbs) is reported. The paper test which detects antibodies against porcine circovirus type 2 (PCV2) using colloidal gold-labelled capsid protein as the antigen probe was applied exclusively in the screening of anti-PCV2 MAbs. It allowed the detection of 118 single cell clones within 30 min using naked eyes. MAbs with specific binding to authentic epitopes on the virus were selected using a blocking strategy in which the antibody was pre-incubated with PCV2 viral sample before applying to the test paper. Five hybridomas secreting MAbs against the capsid protein were obtained, with only three of them capable of binding to PCV2. The results were validated and confirmed using enzyme-linked immunosorbent assay and immunofluorescence assay. The paper test is simple, rapid, and independent on professional technicians and proves to be an excellent approach for the screening of MAbs against specific targets.
Źródło:
Polish Journal of Veterinary Sciences; 2022, 25, 1; 27-34
1505-1773
Pojawia się w:
Polish Journal of Veterinary Sciences
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Development of a SYBR Green I real-time PCR assay for detection of novel porcine parvovirus 7
Autorzy:
Li, Y.D.
Yu, Z.D.
Bai, C.X.
Zhang, D.
Sun, P.
Peng, M.L
Liu, H.
Wang, J.
Wang, Y.
Powiązania:
https://bibliotekanauki.pl/articles/2087207.pdf
Data publikacji:
2021
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
Capsid gene
PPV7
SYBR Green I real-time PCR
Źródło:
Polish Journal of Veterinary Sciences; 2021, 24, 1; 43-49
1505-1773
Pojawia się w:
Polish Journal of Veterinary Sciences
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Effect of Ageratina adenophora on hepatic and pulmonic pathological lesions in horses
Autorzy:
Gu, X.L.
Dai, F.Y.
Xiao, X.
Li, G.Z.
Zhang, L.M.
Qu, W.J.
Powiązania:
https://bibliotekanauki.pl/articles/2087129.pdf
Data publikacji:
2021
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
Ageratina adenophora
liver
lung
horse
toxicity
Źródło:
Polish Journal of Veterinary Sciences; 2021, 24, 4; 611-613
1505-1773
Pojawia się w:
Polish Journal of Veterinary Sciences
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Synergetic effect of microwave blanching and modified atmosphere packaging using laser micro-perforated bags on the storage quality of carrot
Autorzy:
Ezeanaka, M.C.
Zhang, M.
Wang, Y.
Powiązania:
https://bibliotekanauki.pl/articles/2083077.pdf
Data publikacji:
2021
Wydawca:
Polska Akademia Nauk. Instytut Agrofizyki PAN
Tematy:
laser micro-perforated bag
modified atmosphere
packaging
blanching
carrot
storage quality
Opis:
The present study was undertaken to determine the combined effffect of microwave blanching and micro-perforated modifified atmosphere packaging in maximizing shelf life on the basis of quality attributes which were evaluated during storage. The carrots were blanched in water using a microwave at 360 W and then stored in micro-perforated modifified atmosphere packaging. The results indicated that blanched carrots in micro-perforated modifified atmosphere packaging showed an improved storage quality. In particular, it was observed that the fifirmness of the pretreated samples in the micro-perforated package were signifificantly improved. The enzyme activity (peroxidase) initially increased and then decreased with longer storage times. There was also a greater reduction in water content in sample C8, probably due to a higher rate of evaporation through the perforations. Water mobility which comprises tightly bound water, loosely bound water, and immobilized water were observed in all samples, while the free water level was insignificant in pretreated micro-perforated samples. The free water, T24 peak was observed for the control sample which increased with increase in storage duration. Also, the effects of this preservation method on other quality parameters such as β-carotene, water activity, moisture content, and microbial load was limited. However, sample C8 was more effective in retaining quality. Overall, this study demonstrated that blanching carrot in micro-perforated MAP at C8 was the most useful technique in preserving the storage qualities of carrot.
Źródło:
International Agrophysics; 2021, 35, 2; 187-196
0236-8722
Pojawia się w:
International Agrophysics
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Genome-wide association study of terpenoids in resin reveals candidate genes for resin yield in Pinus massoniana
Autorzy:
Bai, Q.
Zhang, Q.
Cai, Y.
Lian, H.
Liu, W.
Luo, M.
Zeng, L.
He, B.
Powiązania:
https://bibliotekanauki.pl/articles/2078149.pdf
Data publikacji:
2020
Wydawca:
Polska Akademia Nauk. Instytut Dendrologii PAN
Tematy:
Pinus massoniana
GC-MS
terpenoid
GWAS
candidate gene
Opis:
Masson pine (Pinus massoniana) is an important species for resin tapping in China. Molecular assisted selection (MAS) has been gradually introduced in to the selection and breeding of masson pine. Until now, the lack of connection between genome information and phenotypic data has hindered the breeding and selection of important chemical compounds of xylem resin. In this study, gas chromatography mass spectrometer (GC-MS) was employed for qualitative and quantitative analysis of chemical compounds of resin collected from 69 masson pine germplasm resources. Then, genome-wide association study (GWAS) was conducted for the terpenoids with higher heritability. A total of 15 primary compounds, including six diterpenes and nine monoterpenes/sesquiterpenes, were detected from xylem resin. By using five GWAS methods, 42 associated SNPs were identified from these masson pines. The P. taeda genome database was used for detecting candidate genes related to the associated SNPs. By using transcriptome data, several members of PKc_like super family, Cytochrome P450, AP2-ERF, F-box, and ABC transporter were found to be highly expressed in the masson pines with higher resin-yielding capacity. The terpenoids associated SNPs can be directly used for MAS. These candidate genes, especially the two F-box members, will contribute to the study of terpenoid biosynthesis mechanisms in the future.
Źródło:
Dendrobiology; 2020, 84; 109-121
1641-1307
Pojawia się w:
Dendrobiology
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Molecular cloning, bioinformation analysis and expression of the strictosidine synthase in Dendrobium officinale
Autorzy:
Zhu, Y.-F.
Fan, H.-H.
Li, D.-H.
Jin, Q.
Zhang, C.-M.
Zhu, L.-Q.
Song, C.
Cai, Y.-P.
Lin, Y.
Powiązania:
https://bibliotekanauki.pl/articles/12715947.pdf
Data publikacji:
2020
Wydawca:
Uniwersytet Przyrodniczy w Lublinie. Wydawnictwo Uniwersytetu Przyrodniczego w Lublinie
Opis:
The enzyme strictosidine synthase (STR, EC: 4.3.3.2) plays a key role in the biosynthetic pathway of terpenoid indole alkaloid (TIA). It catalyzes the condensation of the tryptamine and secologanin to form 3α(S)-strictosidine, which is the common precursor of all TIAs. In this paper, a STR gene designated as DoSTR (GenBank: KX068707) was first cloned and characterized from Dendrobium officinale with rapid amplified cDNA ends method (RACE). DoSTR has a length of 1380bp with 1179bp open reading frame encoding 392 amino acids. BlastP analyses showed that its amino acid sequence was classified into Str_synth superfamily. qRT-PCR showed that DoSTR was expressed in all tissues tested, with a significantly higher level in flower and the lowest in stem. Four different treatments with MeJA, SA, ABA and AgNO₃, respectively, could induce the DoSTR expression to a different extent. And the effect of MeJA was the most obvious and transcript level of DoSTR induced by MeJA was 20.7 times greater than that of control at 48 hours after treatment. Furthermore, it was found that DoSTR was localized in vacuole through transient expression in tobacco. The characterization and expression of DoSTR can help in further studying the role of DoSTR in the biosynthesis of TIAs in D. officinale. This study may throw light on the alkaloid biosynthesis pathway of D. officinale.
Źródło:
Acta Scientiarum Polonorum. Hortorum Cultus; 2020, 19, 3; 111-124
1644-0692
Pojawia się w:
Acta Scientiarum Polonorum. Hortorum Cultus
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Bacterial communities in PM2.5 and PM10 in broiler houses at different broiler growth stages in spring
Autorzy:
Zhang, J.
Li, Y.
Xu, E.
Jiang, L.
Tang, J.
Li, M.
Zhao, X.
Chen, G.
Zhu, H.
Yu, X.
Zhang, X.
Powiązania:
https://bibliotekanauki.pl/articles/2087465.pdf
Data publikacji:
2019
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
bacterial communities
broilers
high-throughput sequencing
particulate matter
Źródło:
Polish Journal of Veterinary Sciences; 2019, 3; 495-504
1505-1773
Pojawia się w:
Polish Journal of Veterinary Sciences
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Development of a blocking immunoperoxidase monolayer assay for differentiation between pseudorabies virus-infected and vaccinated animals
Autorzy:
Wang, Y.B.
Li, Y.H.
Li, Q.M.
Xie, W.T.
Guo, C.L.
Guo, J.Q.
Deng, R.G.
Zhang, G.P.
Powiązania:
https://bibliotekanauki.pl/articles/2087396.pdf
Data publikacji:
2019
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
pseudorabies virus variant strains
anti-pseudorabies virus monoclonal antibody
blocking immunoperoxidase monolayer assay
differentiation between pseudorabies virus-infected and vaccinated animals
Źródło:
Polish Journal of Veterinary Sciences; 2019, 4; 717-723
1505-1773
Pojawia się w:
Polish Journal of Veterinary Sciences
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Genetic diversity and relationship of Hunan province of China local tree peonies based on SSR markers
Autorzy:
Zhang, M.-H.
Jin, X.-L.
Wen, Y.-F.
Shen, S.
Wen-Xing
Wu, S.
Lu, J.-H.
Ye-Ye
Powiązania:
https://bibliotekanauki.pl/articles/12665369.pdf
Data publikacji:
2019
Wydawca:
Uniwersytet Przyrodniczy w Lublinie. Wydawnictwo Uniwersytetu Przyrodniczego w Lublinie
Tematy:
China
Hunan Province
plant cultivation
tree peony
Paeonia suffruticosa
ornamental plant
genetic diversity
genetic relationship
SSR marker
Opis:
Paeonia sect. Moutan is a wide known ornamental plant in the world. The objective of this study was to provide the theoretical basis for scientific preservation and utilization of tree peony resources of Hunan province of China. Simple sequence repeat (SSR) markers were applied to reveal the genetic diversity and relationship of 21 tree peony resources and 45 domestic and foreign tree peony cultivars. Clear bands, the size of which ranged from 115 bp to 379 bp, were detected with 14 primers. In total, 90 alleles were detected and the number of alleles detected with one primer varied between 5 and 13; the number of effective alleles ranged from 1.183 to 2.070; the polymorphism ratio of each locus was 100%. The observed heterozygosity, which ranged from 0.120 to 0.851 with an average of 0.532, was larger than the expected one, which ranged from 0.090 to 0.470 with an average of 0.300. Shannon index ranged from 0.137 to 0.695 and fixation index ranged from −0.332 to −0.869. The results show abundant genetic diversity in tree peony of Hunan province and SSR markers distinguishing homonymous tree peony resources successfully.
Źródło:
Acta Scientiarum Polonorum. Hortorum Cultus; 2019, 18, 4; 213-223
1644-0692
Pojawia się w:
Acta Scientiarum Polonorum. Hortorum Cultus
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Seasonal variations in the abundance and sinking flux of biogenic silica in Daya Bay, Northern South China Sea
Autorzy:
Zhao, X.
Yang, W.
Ma, H.
Li, J.
Chen, M.
Fang, Z.
Zhang, X.
Zeng, J.
Qiu, Y.
Zheng, M.
Powiązania:
https://bibliotekanauki.pl/articles/48777.pdf
Data publikacji:
2019
Wydawca:
Polska Akademia Nauk. Instytut Oceanologii PAN
Tematy:
biogenic silica
flux
thorium
seasonal variation
shallow water
Daya Bay
China Sea
Źródło:
Oceanologia; 2019, 61, 2
0078-3234
Pojawia się w:
Oceanologia
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Ship route planning using historical trajectories derived from AIS Data
Autorzy:
He, Y. K.
Zhang, D.
Zhang, J. F.
Zhang, M. Y.
Li, T. W.
Powiązania:
https://bibliotekanauki.pl/articles/116536.pdf
Data publikacji:
2019
Wydawca:
Uniwersytet Morski w Gdyni. Wydział Nawigacyjny
Tematy:
AIS Data
Automatic Identification System (AIS)
route planning
historical trajectories
AIS messages
marine traffic
Dijkstra algorithm
ant colony algorithm
Opis:
Ship route planning is one of the key issues in enhancing traffic safety and efficiency. Many route planning methods have been developed, but most of them are based on the information from charts. This paper proposes a method to generate shipping routes based on historical ship tracks. The ship's historical route information was obtained by processing the AIS data. From which the ship turning point was extracted and clustered as nodes. The ant colony algorithm was used to generate the optimize route. The ship AIS data of the Three Gorges dam area was selected as a case study. The ships’ optimized route was generated, and further compared with the actual ship's navigation trajectory. The results indicate that there is space of improvement for some of the trajectories, especially near the turning areas.
Źródło:
TransNav : International Journal on Marine Navigation and Safety of Sea Transportation; 2019, 13, 1; 69-76
2083-6473
2083-6481
Pojawia się w:
TransNav : International Journal on Marine Navigation and Safety of Sea Transportation
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
A comprehensive and practical reliability allocation method considering failure effects and reliability costs
Kompleksowa i praktyczna metoda alokacji niezawodności uwzględniająca skutki uszkodzeń i koszty niezawodności
Autorzy:
Yu, H.
Zhang, G.
Ran, Y.
Li, M.
Wang, Y.
Powiązania:
https://bibliotekanauki.pl/articles/301115.pdf
Data publikacji:
2018
Wydawca:
Polska Akademia Nauk. Polskie Naukowo-Techniczne Towarzystwo Eksploatacyjne PAN
Tematy:
reliability allocation
reliability cost
failure modes and effect analysis (FMEA)
relative reliability
fuzzy methods
alokacja niezawodności
koszty niezawodności
analiza przyczynowo-skutkowa
uszkodzenie
względna niezawodność
metody rozmyte
Opis:
In view of the drawbacks in existing allocation methods which are incomplete considerations and poor practicality, a comprehensive fuzzy allocation method considering failure effects and reliability costs is proposed. Fuzzy linguistics and triangular fuzzy numbers are used to evaluate the uncertainty and subjective factors in allocation process. The traditional risk priority numbers (RPNs) are modified to overcome the shortages which are the same factor weights and equal difference of failure effects in original methods. State of the arts, components intricacy and working conditions are used to construct the reliability costs model, which solves the difficulties of costs statistics and avoids the sophisticated calculations which exist in current allocation methods. The relationship between reliability costs and potential risk of subsystem is studied and the value range of it is given in this paper. A case example is given to illustrative the scientificity and practicability of proposed allocation method.
Ze względu na niedostatki istniejących metod alokacji, które nie dają pełnego obrazu problematyki i mają słabe zastosowanie w praktyce, w artykule zaproponowano kompleksową metodę alokacji opartą na logice rozmytej, uwzględniającą skutki uszkodzeń i koszty niezawodności. W pracy wykorzystano lingwistykę rozmytą i trójkątne liczby rozmyte do oceny niepewności i czynników subiektywnych w procesie alokacji. Zmodyfikowano tradycyjny wskaźnik liczby priorytetowej ryzyka (RPN), co pozwoliło na poprawę mankamentów charakteryzujących oryginalną metodę, t.j. takie same współczynniki wagowe i równoważność skutków uszkodzeń o różnym stopniu ciężkości. Na podstawie wiedzy o stanie techniki, złożoności komponentów i warunkach pracy, skonstruowano model kosztów niezawodności, który rozwiązuje trudności dotyczące sporządzania statystyki kosztów i pozwala uniknąć skomplikowanych obliczeń stosowanych w obecnych metodach alokacji. Zbadano związek między kosztami niezawodności a potencjalnym ryzykiem podsystemu, oraz podano jego zakres wartości. Prezentowane studium przypadku demonstruje możliwe zastosowania i efektywność proponowanej metody.
Źródło:
Eksploatacja i Niezawodność; 2018, 20, 2; 244-251
1507-2711
Pojawia się w:
Eksploatacja i Niezawodność
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
A method for tracing exogenous DNA uptake in live spermatozoa and embryos
Autorzy:
Mu, Y.
Wang, J.
Liu, Z.
Zhao, Y.
Zhang, X.
Jiao, M.
Lv, J.
Hao, J.
Kong, Q.
Powiązania:
https://bibliotekanauki.pl/articles/2087751.pdf
Data publikacji:
2018
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
spermatozoa
SMGT
exogenous DNA
IVF
Źródło:
Polish Journal of Veterinary Sciences; 2018, 21, 1; 193-202
1505-1773
Pojawia się w:
Polish Journal of Veterinary Sciences
Dostawca treści:
Biblioteka Nauki
Artykuł
Wyświetlanie 1-15 z 57

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