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    Wyświetlanie 1-8 z 8
    Tytuł:
    Identification of a linear epitope in the capsid protein of goose astrovirus with monoclonal antibody
    Autorzy:
    Dai, G.
    Huang, X.
    Liu, Q.
    Li, Y.
    Zhang, L.
    Han, K.
    Yang, J.
    Liu, Y.
    Xue, F.
    Zhao, D.
    Powiązania:
    https://bibliotekanauki.pl/articles/16647377.pdf
    Data publikacji:
    2022
    Wydawca:
    Polska Akademia Nauk. Czasopisma i Monografie PAN
    Tematy:
    epitope
    goose astrovirus
    capsid protein
    monoclonal antibody
    Opis:
    Goose astrovirus (GoAstV) is a novel avastrovirus that typically causes gosling gout and results in 2 to 20% mortality. GoAstV capsid protein is the sole structural protein, which is responsible for viral attachment, assembly, maturation as well as eliciting host antibodies. However, the epitopes within capsid protein have not been well studied. In this study, a monoclonal antibody, named 1D7, was generated against GoAstV capsid protein by hybridoma technology. Western blot results showed that this MAb could react with recombinant capsid protein expressed in E. coli. Also, it recognized the precursor of capsid protein, VP90 and VP70, in GoAstV-infected cells. Besides, excellent specificity of MAb 1D7 was further demonstrated in indirect immunofluorescence assay and immunohistochemical analysis. Epitope mapping results revealed that MAb 1D7 recognized the epitope 33QKVY 36 within Cap protein. Sequence alignment indicated that 33QKVY 36 is a conserved epitope among the isolates of goose astrovirus type 2 (GoAstV-2), suggesting the potential for its use in GoAstV-2 specific diagnostic assay. These findings may provide some insight into a function of the GoAstV capsid protein and further contribute to the development of diagnostic methods for GoAstV infection.
    Źródło:
    Polish Journal of Veterinary Sciences; 2022, 25, 4; 579-587
    1505-1773
    Pojawia się w:
    Polish Journal of Veterinary Sciences
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    LaCl3 induces genomic DNA instability and increases DNA methylation levels in wheat roots
    Autorzy:
    Lei, X.
    Ma, K.
    Zhang, F.
    Powiązania:
    https://bibliotekanauki.pl/articles/2117694.pdf
    Data publikacji:
    2021
    Wydawca:
    Polska Akademia Nauk. Czasopisma i Monografie PAN
    Tematy:
    cell cycle
    Ca 2+ -channel blocker
    RAPD
    CRED-RA
    Triticum aestivum
    Opis:
    Accumulation of LaCl3 , a well-known Ca 2+ -channel blocker, can inhibit plant growth. However, the current understanding of its effects on gene expression is limited. In this paper, different concentrations of LaCl 3 (0, 0.5, 1.0, 1.5, 2.0 mM) were used to treat germinated wheat (Triticum aestivum L.) seeds for 24 h. The degree of root growth inhibition gradually increased with increasing LaCl 3 concentration. qRT-PCR analysis revealed that the expression of several key genes related to the cell cycle process, such as pcna, mcm2, rdr and cyclin B, were significantly down-regulated. Further analysis of genomic DNA instability using Random Amplified Polymorphic DNA (RAPD) and methylation levels by Coupled Restriction Enzyme Digestion-Random Amplification (CRED-RA) analysis indicated a significant increase in genomic DNA polymorphisms and methylation levels. The results of this study verified that the reasons why LaCl3 treatment can inhibit the growth of wheat roots are as follows: interference in the normal progression of the cell cycle, induction of genomic DNA instability and increase in DNA methylation levels.
    Źródło:
    Acta Biologica Cracoviensia. Series Botanica; 2021, 63, 1; 31-41
    0001-5296
    Pojawia się w:
    Acta Biologica Cracoviensia. Series Botanica
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Experimental and numerical study of the uniaxial compressive stress-strain relationship of a rock mass with two parallel joints
    Autorzy:
    Xiong, L. X.
    Yuan, H. Y.
    Zhang, Y.
    Zhang, K. F.
    Li, J. B.
    Powiązania:
    https://bibliotekanauki.pl/articles/231076.pdf
    Data publikacji:
    2019
    Wydawca:
    Polska Akademia Nauk. Czytelnia Czasopism PAN
    Tematy:
    masa skalna łączona
    złącze równoległe
    siła szczytowa
    jointed rock mass
    parallel joint
    peak strength
    Opis:
    A “rock bridge”, defined as the closest distance between two joints in a rock mass, is an important feature affecting the jointed rock mass strength. Artificial jointed rock specimens with two parallel joint fractures were tested under uniaxial compression and numerical simulations were carried out to study the effects of the inclination of the rock bridge, the dip angle of the joint, rock bridge length, and the length of joints on the strength of the jointed rock mass. Research results show: (1) When the length of the joint fracture, the length of the rock bridge, and the inclination of the rock bridge stay unchanged, the uniaxial compressive strength of the specimen gradually increases as the inclination of the joint fracture increases from 0° to 90°. (2) When the length of the joint fracture, the length of the rock bridge, and the inclination of the joint fracture stay unchanged, the uniaxial compressive strength of the specimen shows variations in trends with the inclination of the rock bridge increasing from 30° to 150° (3). In the case when the joint is angled from the vertical loading direction, when the dip angle of the joint fracture, the inclination of the rock bridge, and the length of the rock bridge stay unchanged, the uniaxial compressive strength of the specimen gradually decreases with an increasing length of joint fracture. When the dip angle of the joint fracture, the inclination of the rock bridge, and the length of the joint fracture stay unchanged, the uniaxial compressive strength of the specimen does not show a clear trend with an increase of the length of the rock bridge.
    Źródło:
    Archives of Civil Engineering; 2019, 65, 2; 67-80
    1230-2945
    Pojawia się w:
    Archives of Civil Engineering
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Ship route planning using historical trajectories derived from AIS Data
    Autorzy:
    He, Y. K.
    Zhang, D.
    Zhang, J. F.
    Zhang, M. Y.
    Li, T. W.
    Powiązania:
    https://bibliotekanauki.pl/articles/116536.pdf
    Data publikacji:
    2019
    Wydawca:
    Uniwersytet Morski w Gdyni. Wydział Nawigacyjny
    Tematy:
    AIS Data
    Automatic Identification System (AIS)
    route planning
    historical trajectories
    AIS messages
    marine traffic
    Dijkstra algorithm
    ant colony algorithm
    Opis:
    Ship route planning is one of the key issues in enhancing traffic safety and efficiency. Many route planning methods have been developed, but most of them are based on the information from charts. This paper proposes a method to generate shipping routes based on historical ship tracks. The ship's historical route information was obtained by processing the AIS data. From which the ship turning point was extracted and clustered as nodes. The ant colony algorithm was used to generate the optimize route. The ship AIS data of the Three Gorges dam area was selected as a case study. The ships’ optimized route was generated, and further compared with the actual ship's navigation trajectory. The results indicate that there is space of improvement for some of the trajectories, especially near the turning areas.
    Źródło:
    TransNav : International Journal on Marine Navigation and Safety of Sea Transportation; 2019, 13, 1; 69-76
    2083-6473
    2083-6481
    Pojawia się w:
    TransNav : International Journal on Marine Navigation and Safety of Sea Transportation
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Ordovician seawater composition : evidence from fluid inclusions in halite
    Autorzy:
    Meng, F.
    Zhang, Y.
    Galamay, A. R.
    Bukowski, K.
    Ni, P.
    Xing, E.
    Ji, L.
    Powiązania:
    https://bibliotekanauki.pl/articles/2059770.pdf
    Data publikacji:
    2018
    Wydawca:
    Państwowy Instytut Geologiczny – Państwowy Instytut Badawczy
    Tematy:
    Ordovician
    fluid inclusions
    halite
    seawater composition
    calcite sea
    Opis:
    Fluid inclusions in halite can directly record the major composition of evaporated seawater; however, Ordovician halite is very rare. The Ordovician is a key time during the evolution history because profound changes occurred in the planet’s ecosystems. Marine life was characterized by a major diversification, the Great Ordovician Biodiversification Event and the Late Ordovician Mass Extinction, the first of the “big five” mass extinctions. However, so far there is no data on the Ordovician seawater. Data from the Ordovician-Silurian boundary were available only. In this study, we report the major compositions from Middle Ordovician halite in China to give the exact composition of Ordovician seawater. The basic ion composition (K+, Mg2+, Ca2+, and SO42-) of inclusion brines was established with the use of ultramicrochemical analysis. The data on the chemical composition of the brines in the primary inclusions indicated that the brines were of Na-K-Mg-Ca-Cl (Ca-rich) type, and cover a huge gap in the evolution of seawater chemistry. The chemical composition of the primary inclusion brine in halite confirmed the earlier results for the Cambrian and Silurian halite originating from other salt basins and the previous speculation of “calcite sea” during the Ordovician, indicating a higher potassium content in the Lower Paleozoic seawater than in the seawater of other periods of the Phanerozoic.
    Źródło:
    Geological Quarterly; 2018, 62, 2; 344--352
    1641-7291
    Pojawia się w:
    Geological Quarterly
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    EGTA, a calcium chelator, affects cell cycle and increases DNA methylation in root tips of Triticum aestivum L.
    Autorzy:
    Zhang, C.
    Shi, W.
    Ma, K.
    Li, H.
    Zhang, F.
    Powiązania:
    https://bibliotekanauki.pl/articles/57455.pdf
    Data publikacji:
    2016
    Wydawca:
    Polskie Towarzystwo Botaniczne
    Opis:
    In this study, when germinated Triticum aestivum L. seeds were treated with 0, 2, 4 and 6 mM ethyl glycol tetraacetic acid (EGTA), root growth was suppressed and the mitotic index decreased. These inhibitory effects were positively correlated with EGTA concentration. RT-PCR analysis revealed that the expression of several gene markers related to the G1/S transition of the cell cycle were significantly downregulated. Confocal microscopy of Fluo-3/AM-stained roots showed chelation of nearly all of the Ca2+ within the root meristematic regions. Both random amplified polymorphic DNA (RAPD) and coupled restriction enzyme digestion-random amplification (CRED-RA) techniques showed significant increases in the levels of genomic DNA polymorphisms and degree of DNA methylation. The study provides information concerning the impact of Ca²+) chelator, EGTA, on the growth, expression of cell cycle transition marker genes, and changes in DNA structure and methylation in the wheat roots.
    Źródło:
    Acta Societatis Botanicorum Poloniae; 2016, 85, 3
    0001-6977
    2083-9480
    Pojawia się w:
    Acta Societatis Botanicorum Poloniae
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Friction Between Footwear and Floor Covered With Solid Particles Under Dry and Wet Conditions
    Autorzy:
    Li, K. W.
    Meng, F.
    Zhang, W.
    Powiązania:
    https://bibliotekanauki.pl/articles/90294.pdf
    Data publikacji:
    2014
    Wydawca:
    Centralny Instytut Ochrony Pracy
    Tematy:
    slip & fall
    friction measurement
    solid contaminants
    Brungraber Mark II
    upadek
    tarcie
    podłoga
    obuwie
    Opis:
    Solid particles on the floor, both dry and wet, are common but their effects on the friction on the floor were seldom discussed in the literature. In this study, friction measurements were conducted to test the effects of particle size of solid contaminants on the friction coefficient on the floor under footwear, floor, and surface conditions. The results supported the hypothesis that particle size of solids affected the friction coefficient and the effects depended on footwear, floor, and surface conditions. On dry surfaces, solid particles resulted in friction loss when the Neolite footwear pad was used. On the other hand, solid particles provided additional friction when measured with the ethylene vinyl acetate (EVA) footwear pad. On wet surfaces, introducing solid particles made the floors more slip-resistant and such effects depended on particle size. This study provides information for better understanding of the mechanism of slipping when solid contaminants are present.
    Źródło:
    International Journal of Occupational Safety and Ergonomics; 2014, 20, 1; 43-53
    1080-3548
    Pojawia się w:
    International Journal of Occupational Safety and Ergonomics
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    New Middle Jurassic tangle-veined flies from Inner Mongolia, China
    Autorzy:
    Zhang, K
    Yang, D.
    Ren, D.
    Ge, F.
    Powiązania:
    https://bibliotekanauki.pl/articles/20019.pdf
    Data publikacji:
    2008
    Wydawca:
    Polska Akademia Nauk. Instytut Paleobiologii PAN
    Tematy:
    Middle Jurassic
    nemestrinid fly
    morphology
    Nemestrinidae
    tangle veined fly
    fossil
    paleontology
    China
    Jurassic
    Ahirmoneura neimengguensis
    Inner Mongolia
    Opis:
    Many Jurassic fossil nemestrinid flies have been found in the Palaearctic region. China is an important locality for fossil nemestrinid flies. Up to now, 2 genera with 3 species have been discovered in China. In the present paper, a third genus and species, Ahirmoneura neimengguensis Zhang, Yang, and Ren, gen. et sp. nov., from the Middle Jurassic Daohugou Formation of China is described. A key to genera of fossil nemestrinid flies from China is given.
    Źródło:
    Acta Palaeontologica Polonica; 2008, 53, 1
    0567-7920
    Pojawia się w:
    Acta Palaeontologica Polonica
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
      Wyświetlanie 1-8 z 8

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