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Wyświetlanie 1-15 z 51
Tytuł:
Identification of a linear epitope in the capsid protein of goose astrovirus with monoclonal antibody
Autorzy:
Dai, G.
Huang, X.
Liu, Q.
Li, Y.
Zhang, L.
Han, K.
Yang, J.
Liu, Y.
Xue, F.
Zhao, D.
Powiązania:
https://bibliotekanauki.pl/articles/16647377.pdf
Data publikacji:
2022
Wydawca:
Polska Akademia Nauk. Czasopisma i Monografie PAN
Tematy:
epitope
goose astrovirus
capsid protein
monoclonal antibody
Opis:
Goose astrovirus (GoAstV) is a novel avastrovirus that typically causes gosling gout and results in 2 to 20% mortality. GoAstV capsid protein is the sole structural protein, which is responsible for viral attachment, assembly, maturation as well as eliciting host antibodies. However, the epitopes within capsid protein have not been well studied. In this study, a monoclonal antibody, named 1D7, was generated against GoAstV capsid protein by hybridoma technology. Western blot results showed that this MAb could react with recombinant capsid protein expressed in E. coli. Also, it recognized the precursor of capsid protein, VP90 and VP70, in GoAstV-infected cells. Besides, excellent specificity of MAb 1D7 was further demonstrated in indirect immunofluorescence assay and immunohistochemical analysis. Epitope mapping results revealed that MAb 1D7 recognized the epitope 33QKVY 36 within Cap protein. Sequence alignment indicated that 33QKVY 36 is a conserved epitope among the isolates of goose astrovirus type 2 (GoAstV-2), suggesting the potential for its use in GoAstV-2 specific diagnostic assay. These findings may provide some insight into a function of the GoAstV capsid protein and further contribute to the development of diagnostic methods for GoAstV infection.
Źródło:
Polish Journal of Veterinary Sciences; 2022, 25, 4; 579-587
1505-1773
Pojawia się w:
Polish Journal of Veterinary Sciences
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Rapid screening of monoclonal antibodies against porcine circovirus type 2 using colloidal gold-based paper test
Autorzy:
Jin, Q.Y.
Feng, L.L.
Wang, Y.B.
Li, P.
Yang, J.F.
Teng, M.
Chai, S.J.
Xing, G.X.
Zhang, G.P.
Powiązania:
https://bibliotekanauki.pl/articles/16539073.pdf
Data publikacji:
2022
Wydawca:
Polska Akademia Nauk. Czasopisma i Monografie PAN
Tematy:
porcine circovirus type 2 (PCV2)
screening of MAbs
paper test
Opis:
A proof of concept for using paper test as a suitable method in the production of monoclonal antibodies (MAbs) is reported. The paper test which detects antibodies against porcine circovirus type 2 (PCV2) using colloidal gold-labelled capsid protein as the antigen probe was applied exclusively in the screening of anti-PCV2 MAbs. It allowed the detection of 118 single cell clones within 30 min using naked eyes. MAbs with specific binding to authentic epitopes on the virus were selected using a blocking strategy in which the antibody was pre-incubated with PCV2 viral sample before applying to the test paper. Five hybridomas secreting MAbs against the capsid protein were obtained, with only three of them capable of binding to PCV2. The results were validated and confirmed using enzyme-linked immunosorbent assay and immunofluorescence assay. The paper test is simple, rapid, and independent on professional technicians and proves to be an excellent approach for the screening of MAbs against specific targets.
Źródło:
Polish Journal of Veterinary Sciences; 2022, 25, 1; 27-34
1505-1773
Pojawia się w:
Polish Journal of Veterinary Sciences
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
The effect of apparent temperature on hospital admissions for cardiovascular diseases in rural areas of Pingliang, China
Autorzy:
Zhai, G.
Tian, Y.
Zhang, K.
Qi, J.
Chai, G.
Powiązania:
https://bibliotekanauki.pl/articles/28761814.pdf
Data publikacji:
2022
Wydawca:
Instytut Medycyny Wsi
Źródło:
Annals of Agricultural and Environmental Medicine; 2022, 29, 2; 281-286
1232-1966
Pojawia się w:
Annals of Agricultural and Environmental Medicine
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Antibacterial spectrum of four compounds from yeasts in koumiss
Autorzy:
Chen, Y.J.
Du, C.G.
Guo, Y.Q.
Zhao, Y.F.
Aorigele, C.
Wang, C.J.
Simujide, H.
Aqima, W.
Zhang, X.Y.
Powiązania:
https://bibliotekanauki.pl/articles/2087160.pdf
Data publikacji:
2021
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
Kluyveromyces marxianus
Saccharomyces cerevisiae
Escherichia coli
minimum inhibition concentration
minimum bactericidal concentration
Źródło:
Polish Journal of Veterinary Sciences; 2021, 24, 2; 167-173
1505-1773
Pojawia się w:
Polish Journal of Veterinary Sciences
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Effect of Ageratina adenophora on hepatic and pulmonic pathological lesions in horses
Autorzy:
Gu, X.L.
Dai, F.Y.
Xiao, X.
Li, G.Z.
Zhang, L.M.
Qu, W.J.
Powiązania:
https://bibliotekanauki.pl/articles/2087129.pdf
Data publikacji:
2021
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
Ageratina adenophora
liver
lung
horse
toxicity
Źródło:
Polish Journal of Veterinary Sciences; 2021, 24, 4; 611-613
1505-1773
Pojawia się w:
Polish Journal of Veterinary Sciences
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
The endemic radiodonts of the Cambrian Stage 4 Guanshan Biota of South China
Autorzy:
Jiao, D.-G.
Pates, S.
Lerosey-Aubril, R.
Ortega-Hernandez, J.
Yang, J.
Lan, T.
Zhang, X-G.
Powiązania:
https://bibliotekanauki.pl/articles/2082327.pdf
Data publikacji:
2021
Wydawca:
Polska Akademia Nauk. Instytut Paleobiologii PAN
Tematy:
Amplectobeluidae
Anomalocarididae
Tamisiocarididae
Paranomalocaris
Burgess Shale-type exceptional
preservation
shallow water
Cambrian
China
Opis:
The Guanshan Biota (South China, Cambrian, Stage 4) contains a diverse assemblage of biomineralizing and non-biomineralizing animals. Sitting temporally between the Stage 3 Chengjiang and Wuliuan Kaili Biotas, the Guanshan Biota contains numerous fossil organisms that are exclusive to this exceptional deposit. The Guanshan Konservat-Lagerstätte is also unusual amongst Cambrian strata that preserve non-biomineralized material, as it was deposited in a relatively shallow water setting. In this contribution we double the diversity of radiodonts known from the Guanshan Biota from two to four, and describe the second species of Paranomalocaris. In addition, we report the first tamisiocaridid from South China, and confirm the presence of a tetraradial oral cone bearing small and large plates in “Anomalocaris” kunmingensis, the most abundant radiodont from the deposit. All four radiodont species, and three genera, are apparently endemic to the Guanshan Biota. When considered in the wider context of geographically and temporally comparable radiodont faunas, endemism in Guanshan radiodonts is most likely a consequence of the shallower and more proximal environment in which they lived. The strong coupling of free-swimming radiodonts and benthic communities underlines the complex relationship between the palaeobiogeographic and environmental distributions of prey and predators. This local adaptation of radiodonts to their prey is highlighted by the frontal appendage morphology of the two species of Paranomalocaris, apparently specialised to different feeding modes, while the recognition of the limited geographic range of some radiodont faunas highlights the importance of exploring as many deposits as possible to fully understand this group.
Źródło:
Acta Palaeontologica Polonica; 2021, 66, 2; 255-274
0567-7920
Pojawia się w:
Acta Palaeontologica Polonica
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Chinese cabbage BrMYB34.2 transcription factor regulates indolic glucosinolates biosynthesis in Arabidopsis
Autorzy:
Zhao, Y.
Zhang, Y.
Guo, X.
Ma, Y.
Zhang, P.
Liu, H.
Liu, G.
Guo, J.
Powiązania:
https://bibliotekanauki.pl/articles/12705984.pdf
Data publikacji:
2020
Wydawca:
Uniwersytet Przyrodniczy w Lublinie. Wydawnictwo Uniwersytetu Przyrodniczego w Lublinie
Opis:
Glucosinolates (GS) are a group of sulfur- and nitrogen-rich plant secondary metabolites that originate from amino acids and exist mainly in plants in the order Brassicales, such as Arabidopsis thaliana (Arabidopsis) and Chinese cabbage (Brassica rapa ssp. pekinensis). To date, several regulatory components responsible for GS biosynthesis have been identified in Arabidopsis. However, the functions of GS biosynthesis regulators in Chinese cabbage have not been clarified. In our current study, a putative ATR1/MYB34 orthologous gene, BrMYB34.2, was isolated from Chinese cabbage leaves. To investigate the function of this gene, we engineered Arabidopsis plants that overexpress BrMYB34.2 ectopically and phenotypic analysis was performed. Moreover, we assayed the accumulation levels of indolic GS (IGS) and aliphatic glucosinolates in transgenic plants and test the expression of key genes of IGS biosynthesis and tryptophan synthesis by Real-time quantitative PCR. And further analysed the resistance of transgenic plants in 5MT stress treatment. The results indicate that ectopic expression of the BrMYB34.2 gene in Arabidopsis was able to up-regulate the accumulation level of IGS due to the increased expression of IGS and Trp biosynthetic genes. Moreover, overexpression of BrMYB34.2 conferred Arabidopsis 5MT resistance. These results suggest that the BrMYB34.2 gene may function as one of the regulators of IGS and Trp biosynthesis in Chinese cabbage.
Źródło:
Acta Scientiarum Polonorum. Hortorum Cultus; 2020, 19, 1; 85-95
1644-0692
Pojawia się w:
Acta Scientiarum Polonorum. Hortorum Cultus
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Bacterial communities in PM2.5 and PM10 in broiler houses at different broiler growth stages in spring
Autorzy:
Zhang, J.
Li, Y.
Xu, E.
Jiang, L.
Tang, J.
Li, M.
Zhao, X.
Chen, G.
Zhu, H.
Yu, X.
Zhang, X.
Powiązania:
https://bibliotekanauki.pl/articles/2087465.pdf
Data publikacji:
2019
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
bacterial communities
broilers
high-throughput sequencing
particulate matter
Źródło:
Polish Journal of Veterinary Sciences; 2019, 3; 495-504
1505-1773
Pojawia się w:
Polish Journal of Veterinary Sciences
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Development of a blocking immunoperoxidase monolayer assay for differentiation between pseudorabies virus-infected and vaccinated animals
Autorzy:
Wang, Y.B.
Li, Y.H.
Li, Q.M.
Xie, W.T.
Guo, C.L.
Guo, J.Q.
Deng, R.G.
Zhang, G.P.
Powiązania:
https://bibliotekanauki.pl/articles/2087396.pdf
Data publikacji:
2019
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
pseudorabies virus variant strains
anti-pseudorabies virus monoclonal antibody
blocking immunoperoxidase monolayer assay
differentiation between pseudorabies virus-infected and vaccinated animals
Źródło:
Polish Journal of Veterinary Sciences; 2019, 4; 717-723
1505-1773
Pojawia się w:
Polish Journal of Veterinary Sciences
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Morphology and developmental traits of the trilobite Changaspis elongata from the Cambrian Series 2 of Guizhou, South China
Autorzy:
Du, G.-Y.
Peng, J.
Wang, D.-Z.
Wang, Q.-J.
Wang, Y.-F.
Zhang, H.
Powiązania:
https://bibliotekanauki.pl/articles/22906.pdf
Data publikacji:
2019
Wydawca:
Polska Akademia Nauk. Instytut Paleobiologii PAN
Opis:
The morphology and ontogeny of the trilobite Changaspis elongata based on 216 specimens collected from the Lazizhai section of the Balang Formation (Stage 4, Series 2 of the Cambrian) in Guizhou Province, South China are described. The relatively continuous ontogenetic series reveals morphological changes, and shows that the species has seventeen thoracic segments in the holaspid period, instead of the sixteen as previously suggested. The development of the pygidial segments shows that their number gradually decreases during ontogeny. A new dataset of well-preserved specimens offers a unique opportunity to investigate developmental traits after segment addition is completed. The ontogenetic size progressions for the lengths of cephalon and trunk show overall compliance with Dyar’s rule. As a result of different average growth rates for the lengths of cephalon, trunk and pygidium, the length of the thorax relative to the body shows a gradually increasing trend; however, the cephalon and pygidium follow the opposite trend. Morphometric analysis across fourteen post-embryonic stages reveals growth gradients with increasing values for each thoracic segment from anterior to posterior. The reconstruction of the development traits shows visualization of the changes in relative growth and segmentation for the different body parts. The new dataset and growth gradient of the trunk suggest that the thoracic segment growth dynamics of early Cambrian to Silurian trilobites follow the same general continuous, steady-state growth gradient decreasing from posterior to anterior.
Źródło:
Acta Palaeontologica Polonica; 2019, 64, 4
0567-7920
Pojawia się w:
Acta Palaeontologica Polonica
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
A novel fibre Bragg grating curvature sensor for structure deformation monitoring
Autorzy:
Liu, M.-Y.
Zhou, S.-G.
Song, H.
Zhou, W.-J.
Zhang, X.
Powiązania:
https://bibliotekanauki.pl/articles/221816.pdf
Data publikacji:
2018
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
fibre Bragg grating
curvature sensor
large structure parts
deformation monitoring
Opis:
Real-time monitoring of deformation of large structure parts is of great significance and the deformation of such structure parts is often accompanied with the change of curvature. The curvature can be obtained by measuring changes of strain, surface curve and modal displacement of the structure. However, many factors are faced with difficulty in measurement and low sensitivity at a small deformation level. In order to measure curvature in an effective way, a novel fibre Bragg grating (FBG) curvature sensor is proposed, which aims at removing the deficiencies of traditional methods in low precision and narrow adjusting. The sensor combines two FBGs with a specific structure of stainless steel elastomer. The elastomer can transfer the strain of the structure part to the FBG and then the FBG measures the strain to obtain the curvature. The performed simulation and experiment show that the sensor can effectively amplify the strain to the FBG through the unique structure of the elastomer, and the accuracy of the sensor used in the experiment is increased by 14% compared with that of the FBG used for direct measurement.
Źródło:
Metrology and Measurement Systems; 2018, 25, 3; 577-587
0860-8229
Pojawia się w:
Metrology and Measurement Systems
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Analysis of future climate scenarios and their impact on agriculture in eastern Arkansas, United States
Analiza scenariuszy klimatycznych i ich wpływ na rolnictwo we wschodniej części Arkansas
Autorzy:
Magugu, J. W.
Feng, S.
Huang, Q.
Zhang, Y.
West, G. H.
Powiązania:
https://bibliotekanauki.pl/articles/292796.pdf
Data publikacji:
2018
Wydawca:
Instytut Technologiczno-Przyrodniczy
Tematy:
agriculture
climate change
climate scenarios
precipitation
temperature
opady
rolnictwo
scenariusze klimatyczne
temperatura
zmiany klimatu
Opis:
Impact of climate change on crop growth is dynamic and difficult to quantify due to heterogeneity of the associated effects and their interactions within the Earth system. The main objective of this study is to establish how future climate change might affect agriculture, through an assessment of temperature and precipitation driven parameters. These include percentage number of rainy days with extreme precipitation, percentage of extreme precipitation relative to wet days, first fall frost days, last spring frost days, growing degree days, growing season length and the total precipitation. Results show modest increase in total precipitation with a slight increase in extreme precipitation, representing up to 2.2% increase by 2060 under representative concentration pathway (RCP 8.5) scenario. There would be late first fall frost days, early last spring frost days and increased growing season length by up to 2 weeks in 2060. The growing degree days are projected to increase under all scenarios for all crops, with cotton showing the largest increase of up to 37% relative to the baseline period.
Wpływ zmian klimatu na wzrost upraw jest dynamiczny i trudny do ilościowej oceny z powodu różnorodności powiązanych efektów i ich interakcji w całym systemie Ziemi. Głównym celem badań prezentowanych w niniejszej pracy było ustalenie, jak zmiany klimatu w przyszłości mogą wpłynąć na rolnictwo, na podstawie oceny parametrów związanych z temperaturą i opadami. Analizowano następujące parametry: udział (w %) dni deszczowych z ekstremalnym opadem, udział ekstremalnych opadów w stosunku do opadów ogółem, pierwsze jesienne dni z mrozem, ostatnie wiosenne dni z mrozem, stopniodni w sezonie wegetacyjnym, długość sezonu wegetacyjnego, i sumę opadów. Wyniki wykazują umiarkowany wzrost całkowitych opadów i niewielki wzrost opadów ekstremalnych – do 2,2% do roku 2060 wg scenariusza Representative Concentration Pathway (RCP 8.5). W 2060 roku pierwsze jesienne dni z mrozem wystąpią później, ostatnie wiosenne dni z mrozem wystąpią wcześniej, a sezon wegetacyjny wydłuży się o ok. 2 tygodnie. Zgodnie ze wszystkimi scenariuszami przewiduje się, że liczba stopniodni w sezonie wegetacyjnym zwiększy się dla wszystkich upraw, a największy przyrost (maksymalnie o 37%) w stosunku do okresu bazowego prognozuje się dla upraw bawełny.
Źródło:
Journal of Water and Land Development; 2018, 37; 97-112
1429-7426
2083-4535
Pojawia się w:
Journal of Water and Land Development
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
On Secrecy Performance for Energy-Harvesting Multi-Antenna Relaying Networks with a Dual-Use Source
Autorzy:
Zhang, J.
Pan, G.
Xie, Y.
Powiązania:
https://bibliotekanauki.pl/articles/226136.pdf
Data publikacji:
2018
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
decode and forward
dual-use
energy harvesting
maximal ratio combining
secrecy outage probability
Opis:
This paper studies the secrecy performance of an energy-harvesting relaying system in the presence of a dualuse source node and an eavesdropper. Specifically, the source has dual roles in the dual-hop communication: 1) to transmit confidential information in the first hop; 2) to generate jamming signal to interfere the eavesdropper in the second hop. Moreover, the multi-antenna relay deploys a power-splitting harvesting scheme to coordinate the information receiving and energy harvesting, and adopts maximal ratio combining technique to process the multiple copies of signals. Considering decode-andforward protocol and transmit antenna selection scheme, we derive an analytical expression for secrecy outage probability, and perform Monte Carlo simulation to validate the analysis. Analytical results show that the SOP performance with the dualuse source node can be effectively improved when the relaydestination channel does not have absolute advantage over the relay-eavesdropper channel.
Źródło:
International Journal of Electronics and Telecommunications; 2018, 64, 1; 33-38
2300-1933
Pojawia się w:
International Journal of Electronics and Telecommunications
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Optimal expression and purification of sapelovirus A structural protein VP1, and its immunogenicity in mice
Autorzy:
Zhao, T.T.
Cui, L.
Chen, L.
Li, J.J.
Liang, Q.L.
Wu, P.J.
Yu, X.Q.
Zhang, Z.H.
Hua, X.G.
Powiązania:
https://bibliotekanauki.pl/articles/2087686.pdf
Data publikacji:
2018
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
sapelovirus A
prokaryotic expression
purification
Źródło:
Polish Journal of Veterinary Sciences; 2018, 21, 3; 573-579
1505-1773
Pojawia się w:
Polish Journal of Veterinary Sciences
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Prevalence of pathogenicity island ETT2 in Escherichia coli isolated from piglets with diarrhea in northeast of China
Autorzy:
Yuan, C.W.
Liu, W.X.
Hou, J.L.
Zhang, L.G.
Wang, G.Q.
Powiązania:
https://bibliotekanauki.pl/articles/2087783.pdf
Data publikacji:
2018
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
Escherichia coli
pathogenicity island
ETT2
piglets
diarrhea
Źródło:
Polish Journal of Veterinary Sciences; 2018, 21, 1; 5-12
1505-1773
Pojawia się w:
Polish Journal of Veterinary Sciences
Dostawca treści:
Biblioteka Nauki
Artykuł
Wyświetlanie 1-15 z 51

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