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Tytuł:
A film stress measurement system applicable for hyperbaric environment and its application in coal and gas outburst simulation test
Autorzy:
Liu, Zhong-Zhong
Wang, Han-Peng
Yuan, Liang
Wang, Wei
Zhang, Chong
Xue, Yang
Powiązania:
https://bibliotekanauki.pl/articles/1848966.pdf
Data publikacji:
2021
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
stress measurement system
sealing technology
hyperbaric environment
coal and gas outburst
gas-solid coupling
Opis:
A film stress measurement system applicable for hyperbaric environment was developed to characterize stress evolution in a physical simulation test of a gas-solid coupling geological disaster. It consists of flexible film pressure sensors, a signal conversion module, and a highly-integrated acquisition box which can perform synchronous and rapid acquisition of 1 kHz test data. Meanwhile, we adopted a feasible sealing technology and protection method to improve the survival rate of the sensors and the success rate of the test, which can ensure the accuracy of the test results. The stress measurement system performed well in a large-scale simulation test of coal and gas outburst that reproduced the outburst in the laboratory. The stress evolution of surrounding rock in front of the heading is completely recorded in a successful simulation of the outburst which is consistent with the previous empirical and theoretical analysis. The experiment verifies the feasibility of the stress measurement system as well as the sealing technology, laying a foundation for the physical simulation test of gas-solid coupled geological disasters.
Źródło:
Metrology and Measurement Systems; 2021, 28, 1; 73-88
0860-8229
Pojawia się w:
Metrology and Measurement Systems
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
A large xenusiid lobopod with complex appendages from the Lower Cambrian Chengjiang Lagerstatte
Autorzy:
Liu, J
Shu, D.
Han, J.
Zhang, Z.
Zhang, X.
Powiązania:
https://bibliotekanauki.pl/articles/21541.pdf
Data publikacji:
2006
Wydawca:
Polska Akademia Nauk. Instytut Paleobiologii PAN
Tematy:
Chengjiang Lagerstatte
Lobopodia
China
arthropod
Jianshanopodia decora
large lobopod
lobopod
body length
Arthropoda
Xenusiidae
Cambrian
paleontology
Lower Cambrian
Opis:
A large lobopod, Jianshanopodia decora gen. et sp. nov., with body length (excluding appendages) about 220 mm from the Lower Cambrian Haikou section, near Kunming, Yunnan, southwest China, shows a mixture of characters, including features of the lobopod Xenusion Pompeckj, 1927, e.g., a large cylindrical body with annulations, stout and strong lobopod appendages each bearing bases of serial tubercles, and of Aysheaia Walcott, 1911, i.e., a pair of large frontal appendages. This suggests that the new genus might be a connecting link between Xenusion and Aysheaia. Besides, Jianshanopodia shares some features with the Early Cambrian stem group arthropod Pambdelurion Budd, 1997, and Kerygmachela Budd, 1993, e.g., the pairs of mid−gut diverticula, the possible presence of tail fan, the mouth cone, the frontal pharyngeal structures and the pharynax are surrounded by the bases of the large frontal appendages. However, compared with a series of segmentally arranged, imbricating, paddle−shaped, movable lateral flaps of both Pambdelurion and Kerygmachela, Jianshanopodia possesses distinct complex appendages with tree−like or lamellate branches. The discovery of this rare transitional form not only throws new light on the early diversification of lobopods, and may also have significance with respect to the origin of arthropods.
Źródło:
Acta Palaeontologica Polonica; 2006, 51, 2
0567-7920
Pojawia się w:
Acta Palaeontologica Polonica
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
A novel combinational evaluation method of voltage and reactive power in regional power grid containing renewable energy
Autorzy:
Ji, Yuqi
Chen, Xuehan
Xiao, Han
Shi, Shaoyu
Kang, Jing
Wang, Jialin
Zhang, Shaofeng
Powiązania:
https://bibliotekanauki.pl/articles/1955201.pdf
Data publikacji:
2021
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
adaptive weighting coefficient
combinational evaluation
renewable energy
typical scenarios
adaptacyjny współczynnik wagowy
ocena kombinacyjna
energia odnawialna
typowy scenariusz
Opis:
The output of renewable energy is strongly uncertain and random, and the distribution of voltage and reactive power in regional power grids is changed with the access to large-scale renewable energy. In order to quantitatively evaluate the influence of renewable energy access on voltage and reactive power operation, a novel combinational evaluation method of voltage and reactive power in regional power grids containing renewable energy is proposed. Firstly, the actual operation data of renewable energy and load demand are clustered based on the K-means algorithm, and several typical scenarios are divided. Then, the entropy weight method (EWM) and the analytic hierarchy process (AHP) are combined to evaluate the voltage qualified rate, voltage fluctuation, power factor qualified rate and reactive power reserve in typical scenarios. Besides, the evaluation results are used as the training samples for back-propagation (BP) neural networks. The proposed combinational evaluation method can calculate the weight coefficient of the indexes adaptively with the change of samples, which simplifies the calculation process of the indexes’ weight. At last, the case simulation of an actual regional power grid is provided, and the historical data of one year is taken as the sample for training, evaluating and analyzing. And finally, the effectiveness of the proposed method is verified based on the comparison with the existing method. The evaluated results could provide reference and guidance to the operation analysis and planning of renewable energy.
Źródło:
Archives of Electrical Engineering; 2021, 70, 4; 925-942
1427-4221
2300-2506
Pojawia się w:
Archives of Electrical Engineering
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
A spatial study on Keshan disease prevalence and selenoprotein P in the Heilongjiang Province, China
Autorzy:
Wang, Yanan
Zhang, Xiao
Wang, Tong
Hou, Jie
Guo, Zhongying
Han, Xiaomin
Zhou, Huihui
Liang, Hong
Xing, Zhifeng
Powiązania:
https://bibliotekanauki.pl/articles/2095499.pdf
Data publikacji:
2021-09-03
Wydawca:
Instytut Medycyny Pracy im. prof. dra Jerzego Nofera w Łodzi
Tematy:
prevalence
biomarker
prevention and control
endemic cardiomyopathy
selenoprotein P
spatial regression analysis
Opis:
ObjectivesFew spatial studies on Keshan disease (KD) prevalence and serum selenoprotein P (SELENOP) levels have been reported in the Heilongjiang Province, China. This study aimed to investigate the spatial relationships between KD prevalence, SELENOP levels, and the socio-economic status for the precise prevention and control of KD.Material and MethodsThe study was carried out in all the 66 KD endemic counties in the Heilongjiang Province using a non-probability sampling method of a key village survey based on county-wide case-searching. The participants completed a questionnaire and had their serum SELENOP levels measured using enzyme-linked immunosorbent assay. Thematic maps were created, and spatial regression analysis was performed by ordinary least squares using ArcGIS 9.0.ResultsOverall, 53 676 residents were surveyed based on case-searching, and blood samples were collected from 409 residents. In total, 50 chronic KD cases were identified with a total prevalence of 9.3/10 000 population. The prevalence in the Tangyuan County was the highest (250/10 000 population). The mean serum SELENOP level was 13.96 mg/l. The spatial regression analysis showed that KD prevalence positively correlated with SELENOP levels and negatively with per capita disposable income among rural residents.ConclusionsThe Tangyuan County should be considered for the precise prevention and control of KD. Further research is necessary to verify the reliability of SELENOP for estimating body selenium levels, and to better understand the relationship between selenium intake and KD in the investigated area.
Źródło:
International Journal of Occupational Medicine and Environmental Health; 2021, 34, 5; 659-666
1232-1087
1896-494X
Pojawia się w:
International Journal of Occupational Medicine and Environmental Health
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
An improved resonant thermal converter based on micro-bridge resonator
Autorzy:
Dong, L.
Han, J.
Zhang, P.
Zhao, Z.
Cheng, B.
Han, D.
Powiązania:
https://bibliotekanauki.pl/articles/220878.pdf
Data publikacji:
2018
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
thermoelectric transfer difference
micro-bridge resonator
thermal converter
bifilar heater
Opis:
This paper presents the design, fabrication and testing of an improved thin-film thermal converter based on an electro-thermally excited and piezo-resistively detected micro-bridge resonator. The resonant thermal converter comprises a bifilar heater and an opposing micro-bridge resonator. When the micro-bridge resonator absorbs the radiant heat from the heater, its axial strain changes, then its resonant frequency follows. Therefore the alternating voltage or current can be transferred to the equivalent DC quantity. A non-contact temperature sensing mechanism eliminates heat loss from thermopiles and reduces coupling capacitance between the temperature sensor and the heater compared with traditional thin-film thermal converters based on thermopiles. In addition, the quasi-digital output of the resonant thin-film thermal converter eliminates such problems as intensity fluctuations associated with analogue signals output by traditional thin-film thermal converters. Using the fast-reversed DC (FRDC) method, the thermoelectric transfer difference, which determines the frequency-independent part of the ac-dc transfer difference, is evaluated to be as low as 1.1 · 10-6. It indicates that the non-contact temperature sensing mechanism is a feasible method to develop a high-performance thermal converter.
Źródło:
Metrology and Measurement Systems; 2018, 25, 4; 715-725
0860-8229
Pojawia się w:
Metrology and Measurement Systems
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Anonymous traffic classification based on three-dimensional Markov image and deep learning
Autorzy:
Tang, Xin
Li, Huanzhou
Zhang, Jian
Tang, Zhangguo
Wang, Han
Cai, Cheng
Powiązania:
https://bibliotekanauki.pl/articles/27311448.pdf
Data publikacji:
2023
Wydawca:
Polska Akademia Nauk. Czasopisma i Monografie PAN
Tematy:
anonymous network
traffic classification
three-dimensional Markov image
output self-attention
deep learning
sieć anonimowa
klasyfikacja ruchu
trójwymiarowy obraz Markowa
samouwaga wyjściowa
uczenie głębokie
Opis:
Illegal elements use the characteristics of an anonymous network hidden service mechanism to build a dark network and conduct various illegal activities, which brings a serious challenge to network security. The existing anonymous traffic classification methods suffer from cumbersome feature selection and difficult feature information extraction, resulting in low accuracy of classification. To solve this problem, a classification method based on three-dimensional Markov images and output self-attention convolutional neural network is proposed. This method first divides and cleans anonymous traffic data packets according to sessions, then converts the cleaned traffic data into three-dimensional Markov images according to the transition probability matrix of bytes, and finally inputs the images to the output self-attention convolution neural network to train the model and perform classification. The experimental results show that the classification accuracy and F1-score of the proposed method for Tor, I2P, Freenet, and ZeroNet can exceed 98.5%, and the average classification accuracy and F1-score for 8 kinds of user behaviors of each type of anonymous traffic can reach 93.7%. The proposed method significantly improves the classification effect of anonymous traffic compared with the existing methods.
Źródło:
Bulletin of the Polish Academy of Sciences. Technical Sciences; 2023, 71, 4; art. no. e145676
0239-7528
Pojawia się w:
Bulletin of the Polish Academy of Sciences. Technical Sciences
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Chlamydial genomic MinD protein does not regulate plasmid-dependent genes like Pgp5
Autorzy:
Sun, Yina
Kong, Jie
Ma, Jingyue
Qi, Manli
Zhang, Ying
Han, Long
Liu, Quanzhong
Liu, Yuanjun
Powiązania:
https://bibliotekanauki.pl/articles/1038373.pdf
Data publikacji:
2018
Wydawca:
Polskie Towarzystwo Biochemiczne
Tematy:
Chlamydia
MinD
Pgp5
Transformation system
Opis:
Chlamydia has a unique intracellular developmental cycle, which has hindered the single protein function study of Chlamydia. Recently developed transformation system of Chlamydia has greatly advanced the chlamydial protein's function research and was used to find that a chlamydial plasmid-encoded Pgp5 protein can down-regulate plasmid-dependent genes. It is assumed, that chlamydial genomic MinD protein has a similar function to Pgp5. However, it is unknown whether MinD protein regulates the same plasmid-dependent genes. We replaced pgp5 gene in the shuttle vector pGFP::CM with minD gene of C. trachomatis (CT0582) or C. muridarum (TC0871). The recombinant plasmid was transformed into plasmid-free organisms-CMUT3 and qRT-PCR was used to detect the transcription level of plasmid-encoded and -dependent genes in these pgp5 deficient organisms. As a readout, GlgA, one of the plasmid-regulated gene products was detected by immunofluorescence assay. After recombination, transformation and plaque purification, the stable transformants CT0582R and TC0871R were generated. In these transformants, the plasmid-dependent genes were up-regulated, alike in the pgp5 premature stop mutant and pgp5 replacement with mCherry mutant. GlgA protein level was also increased in all pgp5 mutants, including CT0582R and TC0871R. Thus, our study showed that genomic MinD protein had different function than Pgp5, which was useful for further understanding the chlamydiae.
Źródło:
Acta Biochimica Polonica; 2018, 65, 3; 425-429
0001-527X
Pojawia się w:
Acta Biochimica Polonica
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Clinical arterial infusion of calcium gluconate: The preferred method for treating hydrofluoric acid burns of distal human limbs
Autorzy:
Zhang, Yuanhai
Ni, Liangfang
Wang, Xingang
Jiang, Ruiming
Liu, Liping
Ye, Chunjiang
Xia, Wenhao
Han, Chunmao
Powiązania:
https://bibliotekanauki.pl/articles/2178799.pdf
Data publikacji:
2014-01-01
Wydawca:
Instytut Medycyny Pracy im. prof. dra Jerzego Nofera w Łodzi
Tematy:
Hydrofluoric Acid
burn
calcium gluconate
arterial infusion
Opis:
Objective: This study was designed to evaluate the efficiency and safety of arterial infusions of calcium gluconate to treat hydrofluoric (HF) acid burns of the distal human limbs. Materials and Methods: Eligible patients with HF burn limbs, collected from January 2008 to October 2011, were given the arterial infusion of calcium gluconate into the injured limbs. The measures of pain were conducted before the infusion, immediately after the infusion, 4 h after the infusion, and 2 days after the infusion by the visual analogy score (VAS). If the VAS score was higher than 4.0 at the time point 4 h after the first infusion, the infusion was repeated. The time of wound healing, and the number and ratio of the cases receiving the surgical operation were also evaluated. Results: A total of 118 patients, male (107 cases) and female (11 cases), were collected, including 64 cases of outpatients and 54 cases of inpatients. The age of the subjects ranged from 16 to 60 years, with the mean age of 37.6. The burn sites were located in the lateral limbs (28 cases) and in the unilateral limbs (90 cases). For 107 cases, the pain scores decreased quickly after the first infusion. The other 11 cases, with the VAS score higher than 4.0 at the time point 4 h after the first infusion, received the second infusion. The average time of wound healing and the ratio of the cases receiving the surgical operation were closely related to the interval from the injury to the reception of infusion. Conclusions: Arterial infusion of calcium gluconate, effectively relieving the pain, blocking wound progressive deepening, and causing no adverse effects, could be the preferential method to treat hydrofluoric acid burns of the distal human limbs.
Źródło:
International Journal of Occupational Medicine and Environmental Health; 2014, 27, 1; 104-113
1232-1087
1896-494X
Pojawia się w:
International Journal of Occupational Medicine and Environmental Health
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Determination of the average maintenance time of CNC machine tools based on type II failure correlation
Określenie średniego czasu konserwacji obrabiarek CNC w oparciu o korelację awarii typu II
Autorzy:
Shen, G.
Zeng, W.
Han, C.
Liu, P.
Zhang, Y.
Powiązania:
https://bibliotekanauki.pl/articles/300892.pdf
Data publikacji:
2017
Wydawca:
Polska Akademia Nauk. Polskie Naukowo-Techniczne Towarzystwo Eksploatacyjne PAN
Tematy:
obrabiarki CNC
średni czas konserwacji
korelacja awarii typu II
DEMATE /ISM
funkcja kopuły
CNC machine tools
Average maintenance time
Type II failure correlation
DEMATEL/ISM
copula function
Opis:
An average maintenance time calculation method based on components failure correlation analysis is proposed to revise the traditional system maintenance time. This paper focus on complex system type II fault correlation, using the Decision-making trial and evaluation laboratory / Interpretative structural model method to divide the fault level of components. And the copula connection function is introduced to calculation of failure rate function of failure correlation components. In addition, the system maintenance time model is established by synthesizing the failure rate function of each unit of the system. Moreover, the average maintenance time under the minimum number of failures is determined. This method shows that the minimum average maintenance time of the proposed system is more reasonable than the traditional one and provides the basis for system and component reliability design.
W artykule zaproponowano metodę obliczania średniego czasu konserwacji, opartą na analizie korelacji uszkodzeń elementów składowych systemu. Metoda ta ma na celu rewizję tradycyjnego czasu konserwacji systemu. Głównym tematem pracy jest korelacja awarii typu II występujących w systemach złożonych. Elementy systemu podzielono ze względu na poziom uszkodzenia przy użyciu metody DEMATEL w połączeniu z interpretacyjnym modelowaniem strukturalnym. Funkcję intensywności skorelowanych uszkodzeń elementów systemu obliczono za pomocą funkcji łączącej (kopuły). Dodatkowo, opracowano model czasu konserwacji systemu poprzez syntezę funkcji intensywności uszkodzeń każdej jednostki systemu. Ponadto, określono średni czas konserwacji dla minimalnej liczby uszkodzeń. Metoda ta pokazuje, że minimalny średni czas konserwacji proponowanego systemu jest korzystniejszy niż tradycyjnie przyjęty i stanowi podstawę do projektowania niezawodności systemu i jego składowych.
Źródło:
Eksploatacja i Niezawodność; 2017, 19, 4; 604-614
1507-2711
Pojawia się w:
Eksploatacja i Niezawodność
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Effect of ethanol-assisted mixing on the selected properties and homogeneity of NR/BR blends
Wpływ mieszania wspomaganego etanolem na wybrane właściwości i jednorodność mieszanek NR/BR
Autorzy:
Meng, Chao
Gu, Zheng
Sui, Jinyong
Wang, Jianfa
Li, Peiyao
Hu, Shaokai
Zhang, Susu
Zhang, Xiaoyi
Wang, Haoyu
Han, Shaolong
Yang, Zhifei
Powiązania:
https://bibliotekanauki.pl/articles/24202782.pdf
Data publikacji:
2023
Wydawca:
Sieć Badawcza Łukasiewicz - Instytut Chemii Przemysłowej
Tematy:
natural rubber
butadiene rubber
blends
dispersion
kauczuk naturalny
kauczuk butadienowy
mieszaniny
dyspersja
Opis:
The effect of the mixing method (normal and ethanol-assisted) on selected properties of natural rubber with butadiene rubber blends (NR/BR 80/20) was investigated. Scanning electron microscopy (SEM), Fourier transform infrared spectroscopy (FTIR) and thermogravimetric analysis (TGA) were used to evaluate the properties. In addition, the mechanical tensile properties, tear resistance and thermal aging, as well as vulcanization characteristic were determined. It was found that ethanol-assisted mixing can significantly improve the mechanical properties and thermal stability of the blends as a result of better dispersion of additives in the rubber matrix.
Zbadano wpływ metody mieszania (tradycyjna i wspomagana etanolem) na wybrane właściwości mieszanin kauczuku naturalnego z kauczukiem butadienowym (NR/BR 80/20). Do oceny właściwości zastosowano skaningową mikroskopię elektronową (SEM), spektroskopię w podczerwieni z transformacją Fouriera (FTIR) oraz analizę termograwimetryczną (TGA). Ponadto oznaczono właściwości mechaniczne przy rozciąganiu, odporność na rozdzieranie i starzenie termiczne, a także właściwości wulkanizacyjne. Stwierdzono, że mieszanie wspomagane etanolem może znacząco poprawić właściwości mechaniczne i stabilność termiczną mieszanin NR/BR, jako efekt lepszej dyspersji dodatków w osnowie gumowej.
Źródło:
Polimery; 2023, 68, 3; 142--148
0032-2725
Pojawia się w:
Polimery
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Electric power steering system controller design using induction machine
Autorzy:
Naibiao, Zhang
Tianfang, Cai
Xuezheng, Han
Powiązania:
https://bibliotekanauki.pl/articles/141715.pdf
Data publikacji:
2019
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
motor induction
electric power
PID control
EPS
Opis:
An electric power steering system (EPS) is a new type of steering system developed after a mechanical hydraulic power system (MHPS) and electric-hydraulic power steering system (EHPS). In order to coordinate and solve the portability and sensitivity of the steering system optimally, taking an induction power steering system as the research object, the control algorithm of induction motor control under the EPS is studied in this paper. In order to eliminate the feed-forward performance degradation caused by the change of feed-forward parameters, an on-line identification algorithm of feed-forward parameters is proposed. It can improve the control performance of online identification among three feed-forward parameters in the T-axle motor, it improves on the robustness of feed-forward control performance, at the same time it also gives simulation and test results. This method can improve the control performance of the three feed-forward parameter online identification of the T-axis motor and improve the robustness of feed-forward control performance. At the same time, simulation and test results are given. The simulation results show that the algorithm can significantly improve the response speed and control accuracy of EPS system control.
Źródło:
Archives of Electrical Engineering; 2019, 68, 2; 279-293
1427-4221
2300-2506
Pojawia się w:
Archives of Electrical Engineering
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Enhancing flotation of smithsonite by using 1,3,5-Triazinane-2,4,6-trithione as sulfidation
Autorzy:
He, Kunzhong
Zhou, Hepeng
Zhang, Yongbing
Tang, Xuekun
Luo, Xianping
Han, Haisheng
Powiązania:
https://bibliotekanauki.pl/articles/1446972.pdf
Data publikacji:
2021
Wydawca:
Politechnika Wrocławska. Oficyna Wydawnicza Politechniki Wrocławskiej
Tematy:
1,3,5-Triazinane-2,4,6-trithione(TMT)
sodium sulfide
flotation
sulfidation
Opis:
1,3,5-Triazinane-2,4,6-trithione (TMT) was used for the first time as a sulfidation agent in the flotation of smithsonite. Results showed that 80.5% recovery rate could be obtained in the presence of TMT $(5 × 10^{-5} mol$ / $L)$ and butyl xanthate $(5 × 10^{-4} mol$ / $L)$. However, the recovery rate was only 59.4% when sodium sulfide $(5 × 10^{-5} mol$ / $L)$ was used. Micro-flotation test and contact angle measurement showed that TMT activation was better than sodium sulfide activation. Besides, the contact angle increased from 32.44° (untreated) to 89.58° (treated with TMT), which was significantly higher than 50.2° (treated with sodium sulfide). Fourier Transform Infrared spectroscopy(FT-IR) and Zeta potential test showed the chemisorption of TMT on the smithsonite surface. The results of ICP spectral detection and solution chemistry calculation revealed that $Zn_3TMT$ complex precipitates in the smithsonite pulp were formed on the mineral surface at pH 6.5. A hydrophobic film was also formed on the mineral surface after TMT treatment, and more adsorption sites were provided for butyl xanthate. Thus, the adsorption of collector was significantly enhanced.
Źródło:
Physicochemical Problems of Mineral Processing; 2021, 57, 5; 1-14
1643-1049
2084-4735
Pojawia się w:
Physicochemical Problems of Mineral Processing
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Identification of a linear epitope in the capsid protein of goose astrovirus with monoclonal antibody
Autorzy:
Dai, G.
Huang, X.
Liu, Q.
Li, Y.
Zhang, L.
Han, K.
Yang, J.
Liu, Y.
Xue, F.
Zhao, D.
Powiązania:
https://bibliotekanauki.pl/articles/16647377.pdf
Data publikacji:
2022
Wydawca:
Polska Akademia Nauk. Czasopisma i Monografie PAN
Tematy:
epitope
goose astrovirus
capsid protein
monoclonal antibody
Opis:
Goose astrovirus (GoAstV) is a novel avastrovirus that typically causes gosling gout and results in 2 to 20% mortality. GoAstV capsid protein is the sole structural protein, which is responsible for viral attachment, assembly, maturation as well as eliciting host antibodies. However, the epitopes within capsid protein have not been well studied. In this study, a monoclonal antibody, named 1D7, was generated against GoAstV capsid protein by hybridoma technology. Western blot results showed that this MAb could react with recombinant capsid protein expressed in E. coli. Also, it recognized the precursor of capsid protein, VP90 and VP70, in GoAstV-infected cells. Besides, excellent specificity of MAb 1D7 was further demonstrated in indirect immunofluorescence assay and immunohistochemical analysis. Epitope mapping results revealed that MAb 1D7 recognized the epitope 33QKVY 36 within Cap protein. Sequence alignment indicated that 33QKVY 36 is a conserved epitope among the isolates of goose astrovirus type 2 (GoAstV-2), suggesting the potential for its use in GoAstV-2 specific diagnostic assay. These findings may provide some insight into a function of the GoAstV capsid protein and further contribute to the development of diagnostic methods for GoAstV infection.
Źródło:
Polish Journal of Veterinary Sciences; 2022, 25, 4; 579-587
1505-1773
Pojawia się w:
Polish Journal of Veterinary Sciences
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Identification of medicinal plant Schisandra chinensis using a potential DNA barcode ITS2
Autorzy:
Li, X.-K.
Wang, B.
Han, R.-C.
Zheng, Y.-C.
Yin, H.-B.
Xu, L.
Zhang, J.-K.
Xu, B.-L.
Powiązania:
https://bibliotekanauki.pl/articles/56996.pdf
Data publikacji:
2013
Wydawca:
Polskie Towarzystwo Botaniczne
Tematy:
identification
internal transcribed spacer
medicinal plant
Schisandra chinensis
DNA barcode
Opis:
To test whether the internal transcribed spacer 2 (ITS2) region is an effective marker for using in authenticating of the Schisandra chinensis at the species and population levels, separately. And the results showed that the wild populations had higher percentage of individuals that had substitution of C→A at site 86-bp than the cultivated populations. At sites 10-bp, 37-bp, 42-bp and 235-bp, these bases of the Schisandra sphenanthera samples differed from that of S. chinensis. Two species showed higher levels of inter-specific divergence than intra-specific divergence within ITS2 sequences. However, 24 populations did not demonstrate much difference as inter-specific and intra-specific divergences were concerned. Both S. chinensis and S. sphenanthera showed monophyly at species level, yet the samples of different populations shown polyphyly at population level. ITS2 performed well when using BLAST1 method. ITS2 obtained 100% identification success rates at the species level for S. chinensis, with no ambiguous identification at the genus level for ITS2 alone. The ITS2 region could be used to identify S. chinensis and S. sphenanthera in the “Chinese Pharmacopoeia”. And it could also correctly distinguish 100% of species and 100% of genera from the 193 sequences of S. chinensis. Hence, the ITS2 is a powerful and efficient tool for species identification of S. chinensis.
Źródło:
Acta Societatis Botanicorum Poloniae; 2013, 82, 4
0001-6977
2083-9480
Pojawia się w:
Acta Societatis Botanicorum Poloniae
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Influence of Annealing Treatment on Deep Drawing Behavior of Q235 Carbon Steel /410/304 Stainless Steels Three-Layer Composite Plate
Autorzy:
Lv, Zehua
Zhang, Zhixiong
Han, Jianchao
Wang, Tao
Powiązania:
https://bibliotekanauki.pl/articles/2106574.pdf
Data publikacji:
2022
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
composite plate
deep drawing
annealing
microstructure
Opis:
Effect of annealing treatment on deep drawing behavior of hot-rolled Q235 carbon steel/410/304 stainless steel three-layer composite plate was investigated. Deep drawability of the unannealed composite plates exhibits a sharp difference for various contact surfaces with the die. The limit drawing ratio (LDR) of the composite plate with the carbon steel contacting the die is 1.75, while it is 1.83 with the stainless steel contacting the die due to the different mechanical responses to the tensile stress at the corner of the die. After annealing at 900°C for 2 h, however, the deep drawabilities of the composite plates both for various contact surfaces with the die are significantly improved and becomes almost identical, which are attributed to the stress relief, the enhanced ductility and the improved interface bonding strength of the hot-rolled component plates during annealing.
Źródło:
Archives of Metallurgy and Materials; 2022, 67, 2; 421--433
1733-3490
Pojawia się w:
Archives of Metallurgy and Materials
Dostawca treści:
Biblioteka Nauki
Artykuł
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