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Tytuł:
Hypoxia-induced programmed cell death in root-tip meristematic cells of Triticum aestivum L.
Autorzy:
Pang, N.
Zhang, F.
Powiązania:
https://bibliotekanauki.pl/articles/19383.pdf
Data publikacji:
2015
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Opis:
In this study, wheat (Triticum aestivum L.) roots were treated with hypoxic water. The staining of cell preparations with DAPI revealed morphological changes of the cells such as nuclear condensation, deformation and fragmentation. Under TEM, cellular membrane shrinkage and breakage, chromatin condensation and apoptotic- like bodies were displayed. The number of mitochondria increased dramatically; their cristae were damaged; the interior became a cavitation and only some flocculent materials were distributed. Indirect immunofluorescence staining indicated that cytochrome C diffused from mitochondria to nucleoplasm and cytoplasm. TUNEL positive nuclei indicated double strand breaks of DNA. DAB staining was used for the identification of hydrogen peroxide and examination showed that the longer the treating time, the darker the staining of the meristematic zones of the roots which suggested the increased accumulation of these Reactive Oxygen Species (ROS). The elevation of hydrogen peroxide production was paralleled with the increase of SOD and POD activities. A negative correlation between the exposure time under hypoxia and the contents of soluble proteins was found. No obvious effect of hypoxia on MDA was established. The obtained results demonstrate that hypoxia causes programmed cell death in the root-tip meristematic cells of Triticum aestivum L. which is most probably attributed to the accumulation of large amounts of ROS.
Źródło:
Acta Biologica Cracoviensia. Series Botanica; 2015, 57, 1
0001-5296
Pojawia się w:
Acta Biologica Cracoviensia. Series Botanica
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
A new sufficient schedulability analysis for hybrid scheduling
Autorzy:
Zhang, F.
Zhai, Y.
Liao, J.
Powiązania:
https://bibliotekanauki.pl/articles/330353.pdf
Data publikacji:
2016
Wydawca:
Uniwersytet Zielonogórski. Oficyna Wydawnicza
Tematy:
scheduling algorithm
real time system
schedulability analysis
preemptive scheduling
earliest deadline first
fixed priority
algorytm szeregowania
system czasu rzeczywistego
analiza szeregowalności
Opis:
Earliest deadline first (EDF) and fixed priority (FP) are the most commonly used and studied scheduling algorithms for real-time systems. This paper focuses on combining the EDF and FP strategies in one system. We provide a new sufficient schedulability analysis for real-time hybrid task systems which are scheduled by EDF and FP. The proposed analysis has a polynomial time complexity and no restrictions on task parameters, where the relative deadline of each task could be less than, equal to, or greater than its period. By extensive experiments, we show that our proposed analysis significantly improves the acceptance ratio compared with the existing results of the sufficient schedulability test for hybrid scheduling systems.
Źródło:
International Journal of Applied Mathematics and Computer Science; 2016, 26, 3; 683-692
1641-876X
2083-8492
Pojawia się w:
International Journal of Applied Mathematics and Computer Science
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
LaCl3 induces genomic DNA instability and increases DNA methylation levels in wheat roots
Autorzy:
Lei, X.
Ma, K.
Zhang, F.
Powiązania:
https://bibliotekanauki.pl/articles/2117694.pdf
Data publikacji:
2021
Wydawca:
Polska Akademia Nauk. Czasopisma i Monografie PAN
Tematy:
cell cycle
Ca 2+ -channel blocker
RAPD
CRED-RA
Triticum aestivum
Opis:
Accumulation of LaCl3 , a well-known Ca 2+ -channel blocker, can inhibit plant growth. However, the current understanding of its effects on gene expression is limited. In this paper, different concentrations of LaCl 3 (0, 0.5, 1.0, 1.5, 2.0 mM) were used to treat germinated wheat (Triticum aestivum L.) seeds for 24 h. The degree of root growth inhibition gradually increased with increasing LaCl 3 concentration. qRT-PCR analysis revealed that the expression of several key genes related to the cell cycle process, such as pcna, mcm2, rdr and cyclin B, were significantly down-regulated. Further analysis of genomic DNA instability using Random Amplified Polymorphic DNA (RAPD) and methylation levels by Coupled Restriction Enzyme Digestion-Random Amplification (CRED-RA) analysis indicated a significant increase in genomic DNA polymorphisms and methylation levels. The results of this study verified that the reasons why LaCl3 treatment can inhibit the growth of wheat roots are as follows: interference in the normal progression of the cell cycle, induction of genomic DNA instability and increase in DNA methylation levels.
Źródło:
Acta Biologica Cracoviensia. Series Botanica; 2021, 63, 1; 31-41
0001-5296
Pojawia się w:
Acta Biologica Cracoviensia. Series Botanica
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Fabrication of Electrical Conductivity and Reinforced Electrospun Silk Nanofibers with MWNTs
Wytwarzanie jedwabnych nanowłókien z dodatkiem nanorurek węglowych
Autorzy:
Zuo, L.
Zhang, F.
Gao, B.
Zuo, B.
Powiązania:
https://bibliotekanauki.pl/articles/233353.pdf
Data publikacji:
2017
Wydawca:
Sieć Badawcza Łukasiewicz - Instytut Biopolimerów i Włókien Chemicznych
Tematy:
silk
multiwalled carbon nanotubes
MWNTs
electrospinning
reinforce electrical conductivity
jedwab
nanowłókna
kwas hialuronowy
elektroprzędzenia
wzmocnienie przewodnictwa elektrycznego
Opis:
Electrospinning is an effective technique for fabricating submicron to nanoscale fibers from synthetic polymer as well as natural proteins. In this study, multiwalled carbon nanotubes (MWNTs) were embedded via electrospinning by adding MWNTs into the spinning dope, and found to be well aligned along the fiber axis in the silk fibroin nanofibers. The morphology and microstructure of the electrospun nanofibers were characterised using a field emission scanning electron microscope (FESEM) and Transmission electron microscopy (TEM). X-ray diffraction (XRD) and TG-DTA were used to study the crystal structure of the silk/MWNTs composite nanofibres, carried out to alter the strength, toughness and electrical conductivity of silk nanofibers by adding a small amount of MWNTs. The electrospun random silk mats with 1% MWNTs had a Young’s modulus, ultimate tensile strength and strain of 107.46 ± 9.15MPa, 9.94 ± 1.2MPa and 9.25 ± 1.5%, respectively, and electrical conductivity increased to 1.2×10-4S/cm. The silk/MWNTs composite nanofibres could potentially be applied in nerve repair materials owing to their excellent mechanical properties and electrical conductivity.
Za pomocą elektroprzędzenia wytworzono jedwabne nanowłókna z dodatkiem nanorurek węglowych. Zbadano wpływ nanorurek węglowych na morfologię, strukturę, właściwości mechaniczne i przewodność elektryczną j nanowłókien. Morfologię i mikrostrukturę otrzymanych nanowłókien scharakteryzowano za pomocą skaningowego mikroskopu elektronowego (FESEM) i transmisyjnej mikroskopii elektronowej (TEM). Wyniki badań rentgenowskich i cieplnych wykazały, że dodanie nanorurek nie wywierało istotnego wpływu na strukturę nanowłókien w porównaniu do niemodyfikowanych nanowłókien fibroinowych. Poprzez dodanie nanorurek uzyskano polepszenie właściwości mechanicznych, została również polepszona przewodność elektryczna nanowłókien. Na podstawie otrzymanych wyników stwierdzono, że jedwabne nanowłókna z dodatkiem nanorurek węglowych mogą być stosowane w materiałach do naprawy nerwów dzięki ich doskonałym właściwościom mechanicznym i przewodności elektrycznej.
Źródło:
Fibres & Textiles in Eastern Europe; 2017, 3 (123); 40-44
1230-3666
2300-7354
Pojawia się w:
Fibres & Textiles in Eastern Europe
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Morphology of the invasive amphiphyte Alternanthera philoxeroides under different water levels and nitrogen concentrations
Autorzy:
Ding, W.
Zhang, H.
Zhang, F.
Wang, L.
Cui, S.
Powiązania:
https://bibliotekanauki.pl/articles/19911.pdf
Data publikacji:
2014
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Źródło:
Acta Biologica Cracoviensia. Series Botanica; 2014, 56, 2
0001-5296
Pojawia się w:
Acta Biologica Cracoviensia. Series Botanica
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Experimental and mathematical investigations on unconfined compressive behaviour of costal soft soil under complicated freezing processes
Autorzy:
Wang, W.
Li, N.
Zhang, F.
Zhou, A.
Chi, S.
Powiązania:
https://bibliotekanauki.pl/articles/259246.pdf
Data publikacji:
2016
Wydawca:
Politechnika Gdańska. Wydział Inżynierii Mechanicznej i Okrętownictwa
Tematy:
costal soft soil
stress-strain curve model
unconfined compressive behavior
freezing-thawing circle
Opis:
In order to properly understand the effect of freezing-thawing circle (FTC) to mechanical behavior of costal soft soil (CSS), unconfined compressive test is conducted. Six kind FTC times are designed from zero to five. The tested data show that: (1) unconfined compressive strength of CCS decreases nonlinearly with more FTC, and the strength after five FTC times becomes about 22% of its original strength without any freezing-thawing experience; (2) stressstrain curves of all unconfined compressive samples can be well fitted by three-parameter hyperbolic model; (3) and relationship between two parameters and FTC times can be fitted by exponent function, while another parameter can be considered as 0.95. Consequently, one composite hyperbolic- exponent empirical formula is established in order to describe freezing-thawing-dependent stress-strain behavior of CSS. Finally, good agreements have been found between tested dada and simulated results.
Źródło:
Polish Maritime Research; 2016, 4; 112-116
1233-2585
Pojawia się w:
Polish Maritime Research
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Explanation of the Optical Spectra and Spin-Hamiltonian Parameters for Nickel(II) in Cadmium Bromide Crystal
Autorzy:
Gong, J.
Wang, L.
Feng, W.
Yang, X.
Zhang, F.
Powiązania:
https://bibliotekanauki.pl/articles/1493631.pdf
Data publikacji:
2011-09
Wydawca:
Polska Akademia Nauk. Instytut Fizyki PAN
Tematy:
71.70.Ch
75.10.Dg
61.72.Bb
76.30.Fc
Opis:
Based on crystal- and ligand-field theory, double-spin-orbital coupling approach was used to analyze the crystal-field energy levels and spin-Hamiltonian parameters of $Ni^{2+}$ ion at trigonal site in $CdBr_2$. The local lattice distortion (Δ R and $τ_{Ni^{2+}}$) is estimated from the crystal field parameters; the crystal field energy Hamiltonian was diagonalized in the full basis consisting of 45 wave functions of the $Ni^{2+}$ ion. Results of calculations are in good agreement with experimental data. The reasonableness of the theoretical results is discussed.
Źródło:
Acta Physica Polonica A; 2011, 120, 3; 497-500
0587-4246
1898-794X
Pojawia się w:
Acta Physica Polonica A
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
EGTA, a calcium chelator, affects cell cycle and increases DNA methylation in root tips of Triticum aestivum L.
Autorzy:
Zhang, C.
Shi, W.
Ma, K.
Li, H.
Zhang, F.
Powiązania:
https://bibliotekanauki.pl/articles/57455.pdf
Data publikacji:
2016
Wydawca:
Polskie Towarzystwo Botaniczne
Opis:
In this study, when germinated Triticum aestivum L. seeds were treated with 0, 2, 4 and 6 mM ethyl glycol tetraacetic acid (EGTA), root growth was suppressed and the mitotic index decreased. These inhibitory effects were positively correlated with EGTA concentration. RT-PCR analysis revealed that the expression of several gene markers related to the G1/S transition of the cell cycle were significantly downregulated. Confocal microscopy of Fluo-3/AM-stained roots showed chelation of nearly all of the Ca2+ within the root meristematic regions. Both random amplified polymorphic DNA (RAPD) and coupled restriction enzyme digestion-random amplification (CRED-RA) techniques showed significant increases in the levels of genomic DNA polymorphisms and degree of DNA methylation. The study provides information concerning the impact of Ca²+) chelator, EGTA, on the growth, expression of cell cycle transition marker genes, and changes in DNA structure and methylation in the wheat roots.
Źródło:
Acta Societatis Botanicorum Poloniae; 2016, 85, 3
0001-6977
2083-9480
Pojawia się w:
Acta Societatis Botanicorum Poloniae
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Experimental Investigation on the Compression Behaviours of 3D Angle‑interlock Woven Composites with Carbons Nanotube under High Strain Rates
Eksperymentalne badanie ściskania kompozytów 3D wzmacnianych tkaninami o skośnym splocie interlokowym modyfikowanych nanorurkami węglowymi przy dużych szybkościach odkształcania
Autorzy:
Ma, P
Jiang, G
Zhang, F
Chen, Q
Miao, X
Cong, H
Powiązania:
https://bibliotekanauki.pl/articles/233089.pdf
Data publikacji:
2015
Wydawca:
Sieć Badawcza Łukasiewicz - Instytut Biopolimerów i Włókien Chemicznych
Tematy:
3D angle-interlock woven composites
carbon nanotubes (CNTs)
impact compression behaviours
high strain rates
badanie ściskania kompozytów 3D
nanorurki węglowe (CNT)
zachowanie wpływu kompresji
duże szybkości odkształcania
Opis:
The compressive properties of 3D angle-interlock woven/epoxy resin composites with various carbon nanotube (CNTs) contents were investigated under quasi-static and high strain rate loading to evaluate the compressive failure modes, which were influenced by various CNT contents and different strain rates. The results indicated that the stress strain curves were strain rate sensitive, and the compressive failure stress of composites with various CNT contents were increased with a change the strain rates and CNT contents. The compressive failure modes of 3D angle-interlock woven composites without CNT tended to be in shear deformation, delamination fibre breakage and matrix crack together, and the failure modes of 3D angle-interlock woven composites with high CNT contents presented delamination and shear deformation.
Badano właściwości ściskające kompozytów 3D wzmacnianych tkaninami o skośnym splocie interlokowym modyfikowanych nanorurkami węglowymi. W celu wyznaczenia uszkodzeń powstałych na skutek ściskania, na które wpływa zawartość nanorurek i szybkości odkształcania, badania przeprowadzono pod obciążeniem quasi-statycznym i przy dużych szybkościach ściskania. Wyniki wykazały, że krzywe ściskania zależały od szybkości odkształcania i zawartości nanorurek. Uszkodzenia mają charakter rozwarstwienia i deformacji pod wpływem naprężeń ścinających.
Źródło:
Fibres & Textiles in Eastern Europe; 2015, 2 (110); 44-50
1230-3666
2300-7354
Pojawia się w:
Fibres & Textiles in Eastern Europe
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Magnetic Properties of Oxygen Deficient Y$\text{}_{0.33}$Sr$\text{}_{0.67}$CoO$\text{}_{3-δ}$
Autorzy:
Zhang, Y. F.
Sasaki, S.
Yanagisawa, O.
Izumi, M.
Powiązania:
https://bibliotekanauki.pl/articles/2047221.pdf
Data publikacji:
2007-01
Wydawca:
Polska Akademia Nauk. Instytut Fizyki PAN
Tematy:
72.80.Ga
75.60.-d
75.70.Rf
81.40.Rs
Opis:
We studied magnetic properties of perovskite-type Y$\text{}_{0.33}$Sr$\text{}_{0.67}$ CoO$\text{}_{2.614}$ and Y$\text{}_{0.33}$Sr$\text{}_{0.67}$CoO$\text{}_{2.707}$ prepared under different sintered temperatures at 1250ºC and 1100ºC, respectively. A dc magnetization jump associated with a large thermal hysteresis around 180 K was found in Y$\text{}_{0.33}$Sr$\text{}_{0.67}$CoO$\text{}_{2.614}$, which indicates a kind of magnetic memory effect. On the other hand, Y$\text{}_{0.33}$Sr$\text{}_{0.67}$CoO$\text{}_{2.707}$ shows a larger magnetization with a small magnetization jump around 200 K and lower Curie temperature than those of Y$\text{}_{0.33}$Sr$\text{}_{0.67}$CoO$\text{}_{2.614}$. Rich Co$\text{}^{3+}$ ions in the low temperature sintered Y$\text{}_{0.33}$Sr$\text{}_{0.67}$CoO$\text{}_{2.707}$ possibly introduce large magnetization and high magnetization jump temperature. This may be coming from a surface/volume effect of Co$\text{}^{3+}$ ions appearing in the polycrystalline sample prepared at low temperature. The ferromagnetism resulted from the different surface/volume ratio contributes to a part of magnetization and may result in the change of the Curie temperature.
Źródło:
Acta Physica Polonica A; 2007, 111, 1; 79-86
0587-4246
1898-794X
Pojawia się w:
Acta Physica Polonica A
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Ship Trajectory Control Optimization in Anti-collision Maneuvering
Autorzy:
Zhang, J. F.
Yang, X. D.
Zhang, D.
Haugen, S.
Powiązania:
https://bibliotekanauki.pl/articles/116373.pdf
Data publikacji:
2013
Wydawca:
Uniwersytet Morski w Gdyni. Wydział Nawigacyjny
Tematy:
anticollision
ship trajectory
genetic algorithm
route optimization
Opis:
A lot of attention is being paid to ship’s intelligent anti‐collision by researchers. Several solutions have been introduced to find an optimum trajectory for ship, such as Game Theory, Genetic or Evolutionary Algorithms and so on. However, ship’s maneuverability should be taken into consideration before their real applications. Ship’s trajectory control in anti‐collision maneuvering is studied in this paper. At first, a simple linear ship maneuverability model is introduced to simulate its movement under different speed and rudder angle. After that, ship’s trajectory control is studied by considering the duration of rudder, operation distance to turning points, and maximum angular velocity. The details for algorithm design are also introduced. By giving some restrictions according to the requirements from COLREGs, the intervals for rudder angle in different circumstances can be determined based on the curves. The results can give very meaningful guidance for seafarers when making decisions.
Źródło:
TransNav : International Journal on Marine Navigation and Safety of Sea Transportation; 2013, 7, 1; 89-93
2083-6473
2083-6481
Pojawia się w:
TransNav : International Journal on Marine Navigation and Safety of Sea Transportation
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Emergency management of maritime accidents in the yangtze river: problems, practice and prospects
Autorzy:
Yan, X.P.
Wu, B.
Zhang, D.
Zhang, J. F.
Powiązania:
https://bibliotekanauki.pl/articles/117335.pdf
Data publikacji:
2017
Wydawca:
Uniwersytet Morski w Gdyni. Wydział Nawigacyjny
Tematy:
inland navigation
emergency management
Yangtze River
maritime accidents
artificial societies
Computational Experiments and Parallel Execution (ACP)
eastern star
inland shipping
oil spill
Opis:
Maritime accidents have received considerable attentions due to the enormous property damage, casualties and serious environmental pollution. This paper first makes statistical analysis of the different types of maritime accidents in the period of 2012 to 2014 in the Yangtze River. Second, the problems of emergency management of maritime accidents are also proposed from the analysis of the major accident “Eastern Star”.. Afterwards, four practice cases, including decision support for maritime accidents, emergency resource allocation, emergency simulation system and effectiveness of emergency management, are introduced to present the insights gained from these practices. Last, in order to address these problems, this paper proposes that an artificial societies, Computational experiments, and Parallel execution (ACP) approach should be introduced to establish an improved management system for maritime accidents in the future, and an ACP based maritime accident emergency management framework is proposed.
Źródło:
TransNav : International Journal on Marine Navigation and Safety of Sea Transportation; 2017, 11, 1; 111-118
2083-6473
2083-6481
Pojawia się w:
TransNav : International Journal on Marine Navigation and Safety of Sea Transportation
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Ship route planning using historical trajectories derived from AIS Data
Autorzy:
He, Y. K.
Zhang, D.
Zhang, J. F.
Zhang, M. Y.
Li, T. W.
Powiązania:
https://bibliotekanauki.pl/articles/116536.pdf
Data publikacji:
2019
Wydawca:
Uniwersytet Morski w Gdyni. Wydział Nawigacyjny
Tematy:
AIS Data
Automatic Identification System (AIS)
route planning
historical trajectories
AIS messages
marine traffic
Dijkstra algorithm
ant colony algorithm
Opis:
Ship route planning is one of the key issues in enhancing traffic safety and efficiency. Many route planning methods have been developed, but most of them are based on the information from charts. This paper proposes a method to generate shipping routes based on historical ship tracks. The ship's historical route information was obtained by processing the AIS data. From which the ship turning point was extracted and clustered as nodes. The ant colony algorithm was used to generate the optimize route. The ship AIS data of the Three Gorges dam area was selected as a case study. The ships’ optimized route was generated, and further compared with the actual ship's navigation trajectory. The results indicate that there is space of improvement for some of the trajectories, especially near the turning areas.
Źródło:
TransNav : International Journal on Marine Navigation and Safety of Sea Transportation; 2019, 13, 1; 69-76
2083-6473
2083-6481
Pojawia się w:
TransNav : International Journal on Marine Navigation and Safety of Sea Transportation
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Experimental and numerical study of the uniaxial compressive stress-strain relationship of a rock mass with two parallel joints
Autorzy:
Xiong, L. X.
Yuan, H. Y.
Zhang, Y.
Zhang, K. F.
Li, J. B.
Powiązania:
https://bibliotekanauki.pl/articles/231076.pdf
Data publikacji:
2019
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
masa skalna łączona
złącze równoległe
siła szczytowa
jointed rock mass
parallel joint
peak strength
Opis:
A “rock bridge”, defined as the closest distance between two joints in a rock mass, is an important feature affecting the jointed rock mass strength. Artificial jointed rock specimens with two parallel joint fractures were tested under uniaxial compression and numerical simulations were carried out to study the effects of the inclination of the rock bridge, the dip angle of the joint, rock bridge length, and the length of joints on the strength of the jointed rock mass. Research results show: (1) When the length of the joint fracture, the length of the rock bridge, and the inclination of the rock bridge stay unchanged, the uniaxial compressive strength of the specimen gradually increases as the inclination of the joint fracture increases from 0° to 90°. (2) When the length of the joint fracture, the length of the rock bridge, and the inclination of the joint fracture stay unchanged, the uniaxial compressive strength of the specimen shows variations in trends with the inclination of the rock bridge increasing from 30° to 150° (3). In the case when the joint is angled from the vertical loading direction, when the dip angle of the joint fracture, the inclination of the rock bridge, and the length of the rock bridge stay unchanged, the uniaxial compressive strength of the specimen gradually decreases with an increasing length of joint fracture. When the dip angle of the joint fracture, the inclination of the rock bridge, and the length of the joint fracture stay unchanged, the uniaxial compressive strength of the specimen does not show a clear trend with an increase of the length of the rock bridge.
Źródło:
Archives of Civil Engineering; 2019, 65, 2; 67-80
1230-2945
Pojawia się w:
Archives of Civil Engineering
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
The study on bactericidal effect and ultrastructural alterations of chlorocresol nanoemulsion disinfectant against Staphylococcus aureus
Autorzy:
Zhang, Y.F.
Sun, Y.W.
Liu, X.H.
An, Z.X.
Yang, X.F.
Powiązania:
https://bibliotekanauki.pl/articles/16647452.pdf
Data publikacji:
2023
Wydawca:
Polska Akademia Nauk. Czasopisma i Monografie PAN
Tematy:
chlorocresol
nanoemulsion
disinfectant
Staphylococcus aureus
bactericidal effect
ultrastructure
Opis:
Chlorocresol nanoemulsion disinfectant (CND) is an environmental disinfectant prepared with nanoemulsion as its drug carrier. This study aimed to investigate the bactericidal effect of CND on Staphylococcus aureus ( S. aureus) and its effect on bacterial ultrastructure. The neutralizing effect of CND against S. aureus was first screened by suspension quantitative evaluation experiment procedure of neutralizer. Disinfection performance was evaluated by the determination of Minimal Inhibitory Concentration (MIC) and Minimum Bactericidal Concentration (MBC), quantitative bactericidal experiment, and comparative experiment of disinfection performance between 0.1% CND and 0.1% chlorocresol aqueous solution. Meanwhile, the effect of CND on the ultrastructure of S. aureus was investigated with scanning electron microscope (SEM) and transmission electron microscope (TEM) to preliminarily explore the bactericidal mechanism. The results showed that 3% Tween-80 in PBS could be screened as the neutralizer of CND against S. aureus. MIC and MBC were 100 μg/mL and 200 μg/mL, respectively. The bactericidal rates were all 100% when 0.06% and 0.08% disinfectant acted for 15 and 5 min, respectively. Furthermore, compared with 0.1% chlorocresol aqueous solution, the bactericidal effect of 0.1% CND was significantly enhanced (p<0.01). After treatment with CND for 10 min, SEM observation showed that the morphology of S. aureus cells were changed and the integrity destroyed. TEM observation showed that the cell shape changed, and the structures of the cell wall, cell membrane and cytoplasm were damaged in varying degrees. CND showed the strong bactericidal effect on S. aureus and could cause ultrastructure alterations of S. aureus.
Źródło:
Polish Journal of Veterinary Sciences; 2023, 26, 1; 13-20
1505-1773
Pojawia się w:
Polish Journal of Veterinary Sciences
Dostawca treści:
Biblioteka Nauki
Artykuł
Wyświetlanie 1-15 z 70

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