- Tytuł:
- Fluorescent in situ hybridization of mitochondrial DNA and RNA
- Autorzy:
-
Alán, Lukáš
Zelenka, Jaroslav
Ježek, Jan
Dlasková, Andrea
Ježek, Petr - Powiązania:
- https://bibliotekanauki.pl/articles/1040297.pdf
- Data publikacji:
- 2010
- Wydawca:
- Polskie Towarzystwo Biochemiczne
- Tematy:
-
molecular beacon fluorescent hybridization probes
nucleoids of mitochondrial DNA
confocal microscopy
transcription and replication intermediates
mitochondrial DNA and RNA - Opis:
- To reveal nucleic acid localization in mitochondria, we designed molecular beacon fluorescent probes against: i) the light strand complementary to ND5 mitochondrial DNA (mtDNA) gene (annealing also to corresponding mRNA); ii) displacement (D) loop 7S DNA (annealing also to parallel heavy strand mtDNA and corresponding light strand transcript); iii) the proximal D-loop heavy strand displaced by the light strand promoter minor RNA. Confocal microscopy demonstrated ND5 probe spreading (less for other probes) in mitochondrial reticulum tubules but upon RNase A treatment all probes contoured mtDNA nucleoid localization. DNase I spread the signal over mitochondrial tubules. Future applications are discussed.
- Źródło:
-
Acta Biochimica Polonica; 2010, 57, 4; 403-408
0001-527X - Pojawia się w:
- Acta Biochimica Polonica
- Dostawca treści:
- Biblioteka Nauki
Artykuł