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Wyszukujesz frazę "Zdunek, K." wg kryterium: Autor


Tytuł:
A 56-year-old man with RT-PCR negative nasopharyngeal swabs with Coronavirus Disease 2019 (COVID-19) Pneumonia
Autorzy:
Dworzańska, A.
Tudrujek-Zdunek, M.
Mosiewicz, J.
Panasiuk, L.
Tomasiewicz, K.
Powiązania:
https://bibliotekanauki.pl/articles/2085530.pdf
Data publikacji:
2020
Wydawca:
Instytut Medycyny Wsi
Tematy:
RT-PCR
pneumonia
Covid-19
Coronavirus Disease 2019
chest computed tomography
real-time-reverse transcription-polymerase chain-reaction
Opis:
Introduction. Diagnostic procedure in Coronavirus Disease 2019 (COVID-19) is based mainly on performing real-time-reverse transcription-polymerase chain-reaction (RT-PCR), which has been accepted as the gold standard method. In some cases, such as mutations of the SARS-CoV-2 genome, variable viral load kinetics or laboratory errors, it can be false-negative. Case report. The case is presented of a 56-year-old man with respiratory tract symptoms, with twice negative results of real-time-reverse transcription-polymerase chain-reaction of nasopharyngeal swabs and positive chest computed tomography, with typical findings for COVID-19 pneumonia. Conclusions. Patients with negative RT-PCR results, but with positive computed tomography findings characteristic for COVID-19, should be treated as well as those infected.
Źródło:
Annals of Agricultural and Environmental Medicine; 2020, 27, 2; 317-318
1232-1966
Pojawia się w:
Annals of Agricultural and Environmental Medicine
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Physical Modelling of the Production of an Alloy Vapour Source for the Synthesis of Dielectric Material
Autorzy:
Nadolski, M.
Stradomski, G.
Zdunek, K.
Okrasa, S.
Powiązania:
https://bibliotekanauki.pl/articles/355587.pdf
Data publikacji:
2020
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
aluminum-silicon alloy
magnetron sputtering processes
gravity casting
die casting
Opis:
The paper reports the results of a physical modelling study of the production of a hypereutectic aluminium alloy to be used formaking an alloy vapour source for operation in the magnetron. Within the study, targets from a hypereutectic aluminium-silicon alloy were made in laboratory conditions. Thus obtained material was subjected to heat treatment, porosity analysis, and the assessment of the microstructure and fitness for being used in the magnetron. The process of melting the hypereutectic Al-Si alloy was carried out at the Department of Foundry of the Czestochowa University of Technology. The investigation into the production of the alloy vapour source for the synthesis of the dielectric material from the hypereutectic aluminium alloy has confirmed.
Źródło:
Archives of Metallurgy and Materials; 2020, 65, 1; 201-206
1733-3490
Pojawia się w:
Archives of Metallurgy and Materials
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Suburban areas of historical value as an important element of the ecological buffer of Warsaw
Podmiejskie obszary o walorach zabytkowych jako istotny element ekologicznej otuliny Warszawy
Autorzy:
Zdunek-Wielgołaska, J.
Kantorowicz, K.
Powiązania:
https://bibliotekanauki.pl/articles/2084006.pdf
Data publikacji:
2020
Wydawca:
Szkoła Główna Gospodarstwa Wiejskiego w Warszawie. Wydawnictwo Szkoły Głównej Gospodarstwa Wiejskiego w Warszawie
Tematy:
suburbs
Warsaw
historic landscape
cultural landscape
natural landscape
climate change
Opis:
The article aims to identify areas of historical, cultural, and landscape values that are located in the suburban area of Warsaw and which may play an essential role in stabilizing the urban ecosystem of Warsaw in the future. Since it turns out that the most effective form of preserving these values is to take them under legal protection, the article shows good and bad practices in this area, identifying the most significant threats and indicating opportunities and scenarios worth considering for the future. Finally, the purpose of the article is to draw attention to the importance of spatial and scenic connections, emphasizing the importance of the surrounding landscape, which was once an immanent feature of, e.g. suburban estates. The hypothesis is that in the face of progressing climate change and melting natural resources, it is worth paying attention to the importance of all urban ecosystem elements, even smaller ones, because they are a component of a larger whole and are required for ecological balance. Existing resources should be strengthened and protected, and new places for protection should be sought. Such sites may be the former foregrounds, the immediate surroundings of monuments, which in the past functioned as larger compositions and should be protected as such.
Źródło:
Acta Scientiarum Polonorum. Architectura; 2020, 19, 4; 27-35
1644-0633
Pojawia się w:
Acta Scientiarum Polonorum. Architectura
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Structure and Electrical Resistivity Dependence of Molybdenum Thin Films Deposited by DC Modulated Pulsed Magnetron Sputtering
Autorzy:
Wicher, B.
Chodun, R.
Nowakowska-Langier, K.
Okrasa, S.
Król, K.
Minikayev, R.
Strzelecki, G.
Zdunek, K.
Powiązania:
https://bibliotekanauki.pl/articles/350987.pdf
Data publikacji:
2018
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
magnetron sputtering
modulated pulse magnetron sputtering
plasma&films characterization
Opis:
This work reports the results of a study of Mo thin films synthesis by DC Pulsed Magnetron Sputtering method (PMS), operating at pulse main frequency of 100 kHz and modulated by the additional modulation frequency, driving in the range of 5-1000 Hz (modulated Pulse Magnetron Sputtering – mPMS). We have studied the influence of mPMS on plasma chemical reactions and mechanisms of layer growth using optical emission spectroscopy technique. Our experiment showed strong influence of mPMS method, on the morphology (scanning electron microscopy), phase composition (X-ray diffractometry) and electric properties (4-point probes method) of nanocrystalline and amorphous Mo films. From the utilitarian point of view, low value of resistivity – 43,2 μΩcm of synthesized Mo films predestines them as back contacts for thin solar cells CIGS. Our results revealed that additional modulation frequency should be considered as an important factor for optimization of films synthesis by means of PMS-based methods.
Źródło:
Archives of Metallurgy and Materials; 2018, 63, 3; 1339-1344
1733-3490
Pojawia się w:
Archives of Metallurgy and Materials
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Microstructure Evolution and Texture Development in a Cu-8.5%AT. AL Material Subjected to Hydrostatic Extrusion
Autorzy:
Jakubowska, D.
Zdunek, J.
Kulczyk, M.
Mizera, J.
Kurzydłowski, K. J.
Powiązania:
https://bibliotekanauki.pl/articles/355019.pdf
Data publikacji:
2016
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
hydrostatic extrusion
texture
XRD
single crystal
Opis:
The aim of the present paper was to investigate microstructure and texture evolution of two single crystals and polycrystal of Cu-8.5%at.Al material. All of mentioned samples were deformed by HE to achieve true strain ε = 1.17. For microstructure analyzes observations by transmission electron microscope (STEM) were done. Crystalline size for samples after SPD were determine using XRD method. The global texture measurements were done using Bruker D8 Discover diffractometer equipped in Cr radiation. Microstructure investigations revealed nanocrystalline structure in single crystals with initial orientations <110> and <100> and polycrystalline Cu-8.5%at.Al material after SPD. The global texture measurements have shown the stability of initial orientation of <100> Cu-8.5%at.Al single crystal after HE, whereas the same SPD process strongly brakes up the orientation <110> Cu-8.5%at. Al single crystal.
Źródło:
Archives of Metallurgy and Materials; 2016, 61, 2B; 933-936
1733-3490
Pojawia się w:
Archives of Metallurgy and Materials
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
The role of magnetic energy on plasma localization during the glow discharge under reduced pressure
Autorzy:
Chodun, R.
Nowakowska-Langier, K.
Zdunek, K.
Okrasa, S.
Powiązania:
https://bibliotekanauki.pl/articles/147975.pdf
Data publikacji:
2016
Wydawca:
Instytut Chemii i Techniki Jądrowej
Tematy:
glow discharge
grounded magnetron
magnetron sputtering
Opis:
In this work, we present the first results of our research on the synergy of fields, electric and magnetic, in the initiation and development of glow discharge under reduced pressure. In the two-electrode system under reduced pressure, the breakdown voltage characterizes a minimum energy input of the electric field to initiate and sustain the glow discharge. The glow discharge enhanced by the magnetic field applied just above the surface of the cathode influences the breakdown voltage decreasing its value. The idea of the experiment was to verify whether the contribution of potential energy of the magnetic field applied around the cathode is sufficiently effective to locate the plasma of glow discharge to the grounded cathode, which, in fact, is the part of a vacuum chamber wall (the anode is positively biased in this case). In our studies, we used the grounded magnetron unit with positively biased anode in order to achieve favorable conditions for the deposition of thin films on fibrous substrates such as fabrics for metallization, assuming that locally applied magnetic field can effectively locate plasma. The results of our studies (Paschen curve with the participation of the magnetic field) seem to confirm the validity of the research assumption. What is the most spectacular – the glow discharge was initiated between introduced into the chamber anode and the grounded cathode of magnetron ‘assisted’ by the magnetic field (discharge did not include the area of the anode, which is a part of the magnetron construction).
Źródło:
Nukleonika; 2016, 61, 2; 191-194
0029-5922
1508-5791
Pojawia się w:
Nukleonika
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Wstęp do analizy odkształceń fotelika samochodowego do przewozu dziecka w trakcie kolizji na podstawie wykonanych symulacji
Introduction in to the analysis of the deformation of a child car seat during a collision based on simulations
Autorzy:
Zdunek, B.
Sawala, K.
Taryma, S.
Powiązania:
https://bibliotekanauki.pl/articles/316885.pdf
Data publikacji:
2016
Wydawca:
Instytut Naukowo-Wydawniczy "SPATIUM"
Tematy:
fotelik
symulacje
przemieszczenia
naprężenia
child car seat
simulations
displacements
stresses
Opis:
W artykule przedstawiono wyniki symulacji statycznej i dynamicznej fotelika samochodowego do przewozu dziecka.
The article presents the results of static and dynamic simulations of the child car seat.
Źródło:
Autobusy : technika, eksploatacja, systemy transportowe; 2016, 17, 8; 216-221
1509-5878
2450-7725
Pojawia się w:
Autobusy : technika, eksploatacja, systemy transportowe
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Evaluation of Physical Properties of Wax Mixtures Obtained From Recycling of Patterns Used in Precision Casting
Ocena właściwości fizycznych mieszanek woskowych uzyskanych na drodze recyklingu modeli dla odlewnictwa precyzyjnego
Autorzy:
Biernacki, R.
Haratym, R.
Bałkowiec, A.
Wawulska-Marek, P.
Matysiak, H.
Zdunek, J.
Kurzydłowski, K. J.
Powiązania:
https://bibliotekanauki.pl/articles/354030.pdf
Data publikacji:
2015
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
investment casting
wax pattern
wax blends
pattern properties
odlewanie
mieszanki wosku
ocena właściwości
mieszanina modelowa
Opis:
The study investigated the properties of selected certified mixtures used to make wax patterns for the production of precision castings for the aerospace industry. In addition, an assessment of the recycled mixtures consisting of certified wax materials recovered during autoclaving was carried out. Hardness was tested via a proposed method based on penetration, creep related deformation, bending strength and linear contraction. The hardness was studied on laboratory specimens and patterns made with the use of injection molding equipment. For these patterns, linear contraction was estimated at variable pressure and for different temperature injection parameters. Deformations connected with creep and resistance were evaluated on cylindrical specimens. Differences in creep resistance in relation to the hardness were observed depending on the type of pattern mixtures. Recycled mixture has a greater resistance and smaller linear contraction than certified mixtures used for making sprue, raisers and other parts of filler system.
W pracy zbadano właściwości wybranych mieszanek modelowych atestowanych stosowanych do wykonywania modeli wytapianych przy produkcji odlewów precyzyjnych dla przemysłu lotniczego. Dodatkowo badaniom poddano masę z recyklingu składająca się z odzyskanych w procesie autoklawizacji mieszanek woskowych atestowanych. Zaproponowano badania twardości metodą penetracji, odkształceń związanych z pełzaniem, wytrzymałości na zginanie oraz skurczu liniowego. Twardość badano na próbkach laboratoryjnych oraz na modelach wykonanych na wtryskarce przemysłowej. Dla tych modeli oszacowano skurcz liniowy przy zmiennych parametrach ciśnienia i temperatury wytrysku. Odkształcenia związane z pełzaniem i wytrzymałość badano na modelach walcowych. Obserwuje się różnice odporności na pełzanie w odniesieniu do twardości w zależności od rodzaju mieszanki modelowej. Masa z recyklingu ma większą odporność i mniejszy skurcz liniowy niż masa atestowana stosowana na układy wlewowe.
Źródło:
Archives of Metallurgy and Materials; 2015, 60, 1; 345-349
1733-3490
Pojawia się w:
Archives of Metallurgy and Materials
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Distribution of classical enterotoxin genes in staphylococci from milk of cows with- and without mastitis and the cowshed environment
Autorzy:
Piechota, M.
Kot, B.
Zdunek, E.
Mitrus, J.
Wicha, J.
Wolska, M.K.
Sachanowicz, K.
Powiązania:
https://bibliotekanauki.pl/articles/31921.pdf
Data publikacji:
2014
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Opis:
The aim of this study was to analyze by PCR 185 isolates of Staphylococcus from milk of cows with- and without mastitis and from the cowsheds environment for their potential ability to produce five classical staphylococcal enterotoxins. Among S. aureus isolates 8 (32%) carried enterotoxin genes and only 2 of them had more than one gene. The enterotoxin genes were detected in 22 (13.7%) coagulase-negative staphylococci (CNS) isolates, among them in 9 (11.4%) isolates of S. xylosus, 5 (16.7%) S. sciuri, 3 (10.3%) S. epidermidis and in 5 (22.7%) Staphylococcus spp. In some CNS 2 or 3 genes were detected simultaneously. Among the investigated enterotoxin genes, sec was the most prevalent (70%). The genes encoding enterotoxin B and D were detected in 5 (16.7%) and 6 (20%) isolates, respectively. The lowest number of isolates had sea and see genes.The genes encoding enterotoxins were often identified in staphylococci from milk of cows with mastitis (73.4% of detected genes), while only 6 (20%) isolates from milk of cows without mastitis and 2 (6.6%) isolates from cowshed environment were positive for enterotoxin genes.The results showed that CNS from bovine milk, like S. aureus, carried enterotoxin genes and may pose a risk for public health.
Źródło:
Polish Journal of Veterinary Sciences; 2014, 17, 3
1505-1773
Pojawia się w:
Polish Journal of Veterinary Sciences
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Influence of Wax Pattern Surface Quality on Prime Coat of Ceramic Mold
Autorzy:
Bałkowiec, A.
Wawulska, P.
Matysiak, H.
Rabajczyk, A.
Smektała, P.
Zdunek, J.
Kurzydłowski, K.
Powiązania:
https://bibliotekanauki.pl/articles/379707.pdf
Data publikacji:
2014
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
casting quality management
wax pattern
technological properties
ceramic shell mold
roughness
X-ray tomography
zarządzanie jakością odlewów
model woskowy
właściwości technologiczne
forma ceramiczna
chropowatość
tomografia rentgenowska
Opis:
The authors have made an attempt to enrich the knowledge about the influence of wax pattern washing process and its influence on the quality of the shell prime coat. Two types of wax were investigated: A7Fr/60 and KC2690. A7Fr60 is used for pattern fabrication, while KC2690 is typical sprue wax. The goal of work was to establish wax solubility accuracy in Trisol 60 Plus and Houghto Clean 530 versus time and influence of dipping time to wax samples surface quality. Additionally, after exposition of wax samples, their surface morphology was characterized with the use of laser profilometry and surface roughness measurement. The quality of formed prime coat was established by X-ray tomography. The measurement of wetting angle of the wax by binder was conducted. The results have shown that the main factor which influences the quality of the prime coat is surface wettability rather than wax surface roughness.
Źródło:
Archives of Foundry Engineering; 2014, 14, 1 spec.; 67-72
1897-3310
2299-2944
Pojawia się w:
Archives of Foundry Engineering
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Ocena możliwości wtórnego zastosowania odpadowych mas woskowych w technologii odlewania precyzyjnego
Assessment of the Possibility of Application of Waste Wax in the Investment Casting Technology
Autorzy:
Wawulska-Marek, P.
Białkowiec, A.
Matysiak, H.
Zdunek, J.
Kurzydłowski, K. J.
Powiązania:
https://bibliotekanauki.pl/articles/382367.pdf
Data publikacji:
2014
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
masy modelowe
woski
regeneracja
odlewanie precyzyjne
mass model
waxes
regeneration
precision casting
Opis:
W pracy podjęto próbę określenia możliwości odzysku odpadowych mas modelowych powstających podczas wytopu modeli woskowych z form ceramicznych. W pracy porównane zostały właściwości technologiczne komercyjnej masy woskowej stosowanej na modele układów wlewowych oraz właściwości odzyskanej masy woskowej. Ocenie poddano jakość technologiczną uzyskanych odpadowych mieszanek woskowych. Zastosowano szereg technik badawczych takich jak: TGA, DSC, pomiary lepkości, FTIR, twardość, a uzyskane wyniki poddano analizie pod kątem możliwości wtórnego zastosowania odzyskanych mas woskowych do wytwarzania układów wlewowych.
The study attempts to determine the possibility of recovery of waste generated during the melting of wax patterns from ceramic moulds. In this work were compared to the technological characteristics of commercial kinds wax used for gating circuit patterns and the properties of the recovered wax. Quality assessment of technological obtained waste wax blends. A range of research techniques such as TGA, DSC, viscosity measurements, FTIR, hardness, and the results were analyzed for the possibility of re-use of recovered wax for manufacturing gating systems.
Źródło:
Archives of Foundry Engineering; 2014, 14, 2 spec.; 89-94
1897-3310
2299-2944
Pojawia się w:
Archives of Foundry Engineering
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Analiza obciążeń działających na dzieci w fotelikach zamocowanych przodem i tyłem do kierunku jazdy w czasie zderzenia czołowego
Analysis of loads acting on children in child restraint system fixed front and REAR-facing during a frontal collision
Autorzy:
Zdunek, B.
Landowski, M.
Taryma, S.
Woźniak, R.
Imielińska, K.
Muszyński, A.
Powiązania:
https://bibliotekanauki.pl/articles/309866.pdf
Data publikacji:
2013
Wydawca:
Instytut Naukowo-Wydawniczy "SPATIUM"
Tematy:
foteliki dziecięce
zderzenie czołowe
analiza obciążeń
child restraint system
frontal collision
analysis of loads acting
Opis:
W artykule omówione zostały podstawowe problemy ochrony dzieci w czasie kolizji drogowych. Przedstawione zostały dwa sposoby mocowania fotelików: przodem i tyłem do kierunku jazdy. Opisane i porównane zostały przebiegi obciążeń głowy w czasie uderzenia pojazdu w sztywną ścianę betonową.
Paper describes the basic problems of children protection during collisions. Presented and compared were head deceleration during vehicle impact with a rigid concrete wall for two ways of fixing child seats: front and rear-facing.
Źródło:
Autobusy : technika, eksploatacja, systemy transportowe; 2013, 14, 10; 311-314
1509-5878
2450-7725
Pojawia się w:
Autobusy : technika, eksploatacja, systemy transportowe
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Virulence factors and ability of staphylococci from bovine milk and the cowshed environment to biofilm formation
Autorzy:
Kot, B
Binek, T.
Piechota, M.
Wolska, K.M.
Zdunek, E.
Platkowska, K.
Powiązania:
https://bibliotekanauki.pl/articles/31690.pdf
Data publikacji:
2013
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Opis:
The aim of this study was to examine virulence factors and the ability of S. aureus and CNS species isolated from milk of cows with mastitis to form biofilm, and to compare them with virulence factors of staphylococci from milk of cows without mastitis and cowshed environment. Most of S. aureus strains from cows with mastitis showed haemolytic activity (93.9%), among them 72.7% and 21.2% produced α- and β-haemolysin, respectively. S. aureus from cows with mastitis symptoms produced proteases (above 48%) and esterase (42.4%). The highly significant relationship between the number of S. xylosus strains producing haemolysins (62%) and the origin of these strains from milk of cows with mastitis was observed. The ability to produce proteases was significantly associated with S. sciuri from milk of cows with mastitis. The ability of biofilm formation by staphylococcal strains from milk of cows with mastitis was greater than in strains from milk of cows without mastitis and the difference was significant (p≤0.05). The highest percentage of strains from milk of cows with mastitis were weak biofilm formers (48.6%), while 40% and 11.4% of strains were moderate and strong biofilm producers, respectively. S. xylosus showed the highest ability to form biofilm, while the lowest ability to form biofilm was observed in S. aureus and S. epidermidis. In conclusion, production of exotoxins and enzymes, and ability of biofilm formation shown by many CNS isolated from milk of cows with mastitis symptoms indicates that these features are important in pathogenesis of this disease.
Źródło:
Polish Journal of Veterinary Sciences; 2013, 16, 4
1505-1773
Pojawia się w:
Polish Journal of Veterinary Sciences
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Antimicrobial resistance and genotypes of staphylococci from bovine milk and the cowshed environment
Autorzy:
Kot, B.
Piechota, M.
Antos-Bielska, M.
Zdunek, E.
Wolska, K.M.
Binek, T.
Olszewska, J.
Gulinski, P.
Trafny, E.A.
Powiązania:
https://bibliotekanauki.pl/articles/30335.pdf
Data publikacji:
2012
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Opis:
Investigation of antimicrobial resistance and genetic relatedness of staphylococci from milk of cows with mastitis and cowshed environment was the aim of this study. Antimicrobial resistance against 14 antimicrobials were determined by using a disc diffusion method. Genetic similarity between the most frequently isolated species was analysed by PFGE (pulsed-field gel electrophoresis). Haemolytic activity, DNase, protease and esterase production was also investigated. Coagulase-negative Staphylococcus species were isolated from 30.8% of milk samples from cows with mastitis. The most frequently isolated species was Staphylococcus xylosus and yield of these organisms was significantly associated with milk of mastitis cows. S. epidermidis was a predominant penicillin-resistant species. High frequency of resistance to lincomycin was observed among isolates of S. sciuri (54.2%) and S. xylosus (25.9%) from cows with mastitis. PFGE (pulsed-field gel electrophoresis) analysis of 29 Staphylococcus aureus isolates showed the presence of 17 PFGE pulsotypes. Isolates of S. sciuri (n=36) had unique PFGE patterns. Some S. xylosus isolates from milk and milker’s hands had the same PFGE pulsotypes, and this observation could indicate that dairyman may be a potential source of the infection. The pulsotype of each of the remaining isolates of S. xylosus suggested that they might have come from common environmental sources; however, these isolates differed in antibiotic resistance pattern or virulence traits. Therefore, knowledge about antibiotic sensitivity pattern and virulence factors of a CNS isolate, besides its genotype, may be informative in tracking the source of the infection.
Źródło:
Polish Journal of Veterinary Sciences; 2012, 15, 4
1505-1773
Pojawia się w:
Polish Journal of Veterinary Sciences
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Phenotypic and genotypic antimicrobial resistance of staphylococci from bovine milk
Autorzy:
Kot, B.
Piechota, M.
Wolska, K.M.
Frankowska, A.
Zdunek, E.
Binek, T.
Klopotowska, E.
Antosiewicz, M.
Powiązania:
https://bibliotekanauki.pl/articles/31274.pdf
Data publikacji:
2012
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Opis:
The aim of this study was to examine phenotypic and genotypic antimicrobial resistance of staphylococci from milk samples from cows with subclinical and clinical mastitis and from cows without mastitis symptoms to methicillin, tetracyclines, macrolides and lincosamides (ML). Of 207 strains, including 34 S. aureus and 173 coagulase-negative staphylococci (CNS), 11 (6.4%) CNS strains were phenotypically resistant to methicillin. The mecA gene was detected by PCR only in two S. xylosus strains and one strain of S. epidermidis and S. simulans. No methicillin-resistant S. aureus strains were observed. In methicillin-resistant strains with mecA, gene resistance to other investigated antibiotics was not observed. Phenotypic resistance to tetracycline was detected in 11.0% of CNS strains and 47.4% of them carried the tetK gene. Of 173 CNS strains studied, 27 (15.6%) were resistant to at least one ML antibiotic. The resistance gene ermC was detected in 55.5% of the 27 ML-resistant strains. The ermA and ermB genes were detected in 14.8% and 11.1% of ML-resistant CNS strains, respectively. Antimicrobial resistance to methicillin, tetracyclines and macrolides was detected more frequently in staphylococcal strains from clinical mastitis compared to animals with subclinical symptoms and without mastitis, while the resistance to lincosamides showed a similar frequency in all groups of cows. In conclusion, CNS species from bovine milk differ in phenotypic and genotypic antimicrobial resistance profiles, and the use of PCR technique alone for the detection of methicillin, macrolide, lincosamide and tetyracycline resistance in CNS from cattle is not reliable.
Źródło:
Polish Journal of Veterinary Sciences; 2012, 15, 4
1505-1773
Pojawia się w:
Polish Journal of Veterinary Sciences
Dostawca treści:
Biblioteka Nauki
Artykuł

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