- Tytuł:
- Detection of Potato Virus Y (PVY) by Reverse-Transcription Loop-mediated Nucleic Acid Amplification (RT-lamp)
- Autorzy:
-
Treder, Krzysztof
Chołuj, Joanna
Zacharzewska, Bogumiła
Mielczarek, Mateusz - Powiązania:
- https://bibliotekanauki.pl/articles/2199694.pdf
- Data publikacji:
- 2017-06-20
- Wydawca:
- Instytut Hodowli i Aklimatyzacji Roślin
- Tematy:
-
detection
plant pathogen
RT-LAMP - Opis:
- Potato virus Y (PVY), a type member of the genus Potyvirus (family Potyviridae), is currently the most important virus infecting the potato crop. PVY is also a dangerous pathogen of the tomato, pepper, and tobac-co. The reverse transcription loop-mediated amplification (RT-LAMP) is gaining recognition as a good alter-native to RT-PCR in diagnosing plant viruses. Here, we provide a detailed description of a simple protocol for fast and sensitive detection of PVY by the RT-LAMP assay, which can be easily adapted to detect other plant pathogens, harboring both RNA and DNA genomes.
- Źródło:
-
Plant Breeding and Seed Science; 2017, 75; 77-85
1429-3862
2083-599X - Pojawia się w:
- Plant Breeding and Seed Science
- Dostawca treści:
- Biblioteka Nauki
Artykuł