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    Wyświetlanie 1-26 z 26
    Tytuł:
    Molecular characterization and expression analysis of the NLR family CARD containing five transcripts in the pig
    Autorzy:
    Yang, Q.Y.
    Chen, T.
    Chen, Y.B.
    Lan, D.L.
    Powiązania:
    https://bibliotekanauki.pl/articles/32088.pdf
    Data publikacji:
    2016
    Wydawca:
    Polska Akademia Nauk. Czytelnia Czasopism PAN
    Opis:
    The NOD-like receptor (NLR) family caspase recruitment domain-containing 5 (NLRC5) is one of the newly discovered and largest NLR family members. The NLRC5 has recently received extensive attention because of its important role in regulating innate and adaptive immune responses. The NLRC5 in many vertebrates, such as humans, mice, cattle, and horses, has already been proven and studied. However, the NLRC5 gene characteristics of pigs remain unclear. Thus, we completely cloned the NLRC5 cDNA sequence of the pig using the rapid amplification of cDNA ends(RACE) technology. A characteristic and tissue expression analysis was also conducted on the pig sequence. The sequence analysis showed that the complete cDNA sequence of the NLRC5 of the pig is 6638 bp, and the open reading frame is 5538 bp which encoded 1846 amino acids. The protein prediction analysis indicates that the overall performance of the NLRC5 protein of the pig is hydrophilic and possesses a typical nucleotide binding and oligomerization domain(NBD) and 20 leucine-rich repeats(LRRs). The homology analysis result indicates that the NLRC5 transcript in pigs is highly homologous to cattle, sheep, macaques, and humans, and accounts for around 80%. The genetic evolutionary tree analysis shows that the NLRC5 transcript in pigs has the closest evolutionary relationship with cattle and sheep. Further tissue expression analysis shows that immune organ systems (e.g., lymph node and spleen) and mucosa organs (e.g., intestinal lymph node, stomach, and lungs) possess high expressions with NLRC5 mRNA. The result of this study indicates that the NLRC5 transcript in pigs is relatively conservative among mammals and may play a vital role in immune reaction, which provides a basis for further studies on the NLRC5 function in the pig immune system and the role in comparative immunity.
    Źródło:
    Polish Journal of Veterinary Sciences; 2016, 19, 4
    1505-1773
    Pojawia się w:
    Polish Journal of Veterinary Sciences
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Preparation and cytological study of collagen/nano-hydroxyapatite/graphene oxide composites
    Autorzy:
    Wang, J.
    Wang, Y.
    Liu, D.
    Yang, Q.
    Huang, C.
    Yang, C.
    Zhang, Q.
    Powiązania:
    https://bibliotekanauki.pl/articles/307450.pdf
    Data publikacji:
    2018
    Wydawca:
    Politechnika Wrocławska. Oficyna Wydawnicza Politechniki Wrocławskiej
    Tematy:
    nanohydroksyapatyt
    tlenek grafitu
    mineralizacja
    kolagen
    nano-hydroxyapatite
    graphene oxide
    biomimetic mineralization
    collagen
    Opis:
    Biomimetic mineralized composite scaffolds are widely used as natural bone substitute materials in tissue engineering by inducing and assembling bonelike apatite. In this study, the single lamellar structure of graphene oxide (GO) powder was prepared via an improved Hummers’ method. Methods: To better mimic natural bone, the collagen (COL)/Nano-hydroxyapatite (nHA)/graphene oxide (GO) composite material was prepared by simulated body fluid (SBF) method using COL/GO as a matrix template. Hydroxyapatite (HA) with calcium ion deficiency was achieved via biomimetic mineralization, and it had properties closer to those of natural bone than pure HA has. Results: The mineralized COL/nHA/GO composites exhibited loose porous structures, and the connectivity of the holes was good and thus beneficial to the exchange of nutrients and excreted metabolites. Conculsions: Antibacterial and MTT experiment confirmed that the COL/nHA/GO composite material had excellent antibacterial property and biocompatibility. Hence, these results strongly suggested the mineralized COL/nHA/GO composite is a good candidate biomaterial to be applied in bone tissue engineering.
    Źródło:
    Acta of Bioengineering and Biomechanics; 2018, 20, 4; 65-74
    1509-409X
    2450-6303
    Pojawia się w:
    Acta of Bioengineering and Biomechanics
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Effects of seminal plasma concentration on sperm motility and plasma and acrosome membrane integrity in chilled canine spermatozoa
    Autorzy:
    Pan, C.
    Wu, Y.
    Yang, Q.
    Ye, J.
    Powiązania:
    https://bibliotekanauki.pl/articles/2087767.pdf
    Data publikacji:
    2018
    Wydawca:
    Polska Akademia Nauk. Czytelnia Czasopism PAN
    Tematy:
    seminal plasma
    chill
    canine
    sperm
    Źródło:
    Polish Journal of Veterinary Sciences; 2018, 21, 1; 133-138
    1505-1773
    Pojawia się w:
    Polish Journal of Veterinary Sciences
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    A numerical approach in applying panel method for the added mass of a group of sections in fluids
    Autorzy:
    Zhang, D.
    Li, P.
    Wang, Q.
    Yang, Y.
    Powiązania:
    https://bibliotekanauki.pl/articles/38695713.pdf
    Data publikacji:
    2023
    Wydawca:
    Instytut Podstawowych Problemów Techniki PAN
    Tematy:
    numerical method
    panel method
    fluid-structure interaction
    confined fluid
    fluid mass coefficients
    Opis:
    This paper firstly expands an efficient numerical methodology developed from the source panel method to the added mass calculation of long column systems in fluid. Structures submerged in fluid are considered as two-dimensional and are discretized into a number of source panels. The influence coefficient matrices and the potential function are then calculated and the fluid forces are calculated by the unsteady Bernoulli equation. Finally, we utilize this present method to calculate the mass coefficients of some typical problems, which effectively verifies its feasibility and accuracy. This method takes into account both applicability and computational efficiency. On the one hand, in contrast to the analytical method which is only applicable to specific cross-sections, this method is applicable to arbitrary boundaries with C0 continuity in mathematics. On the other hand, this method requires less mesh and computation than commercial software. This paper extends the application of the source panel method which is widely used in aerodynamics to provide a reference for added mass calculation problems in engineering.
    Źródło:
    Archives of Mechanics; 2023, 75, 1-2; 151-168
    0373-2029
    Pojawia się w:
    Archives of Mechanics
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Microstructure and Mechanical Behavior of Mg-0.5Si-xSn Alloys
    Autorzy:
    Fu, X.
    Yang, Y.
    Ma, Q. Y.
    Peng, X.
    Xu, T.
    Powiązania:
    https://bibliotekanauki.pl/articles/379670.pdf
    Data publikacji:
    2017
    Wydawca:
    Polska Akademia Nauk. Czytelnia Czasopism PAN
    Tematy:
    low silicon magnesium alloy
    eutectic Mg2Si
    microstructure behavior
    mechanical behavior
    stop magnezu
    stop Mg2Si
    zachowanie mechaniczne
    Opis:
    Mg-0.5Si-xSn (x=0.95, 2.9, 5.02wt.%) alloys were cast and extruded at 593K (320 ºC) with an extrusion ratio of 25. The microstructure and mechanical properties of as-cast and extruded test alloys were investigated by OM, SEM, XRD and tensile tests. The experimental results indicate that the microstructure of the Mg-0.5Si-xSn alloys consists of primary α-Mg dendrites and an interdendritic eutectic containing α-Mg, Mg2Si and Mg2Sn. There is no coarse primary Mg2Si phase in the test alloys due to low Si content. With the increase in the Sn content, the Mg2Si phase was refined. The shape of Mg2Si phase was changed from branch to short bar, and the size of them were reduced. The ultimate tensile strength and yield strength of Mg-0.52Si-2.9Sn alloy at the temperature of 473K (200 ºC) reach 133MPa and 112MPa respectively. Refined eutectic Mg2Si phase and dispersed Mg2Sn phase with good elevated temperature stability are beneficial to improve the elevated temperature performance of the alloys. However, with the excess addition of Sn, large block-like Mg2Sn appears around the grain boundary leading to lower mechanical properties.
    Źródło:
    Archives of Foundry Engineering; 2017, 17, 4; 179-184
    1897-3310
    2299-2944
    Pojawia się w:
    Archives of Foundry Engineering
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Fatigue life prediction of umbilicals considering non-linear stress
    Autorzy:
    Lu, Q.
    Yin, Y.
    Yang, Z.
    Chen, J.
    Yan, J.
    Yue, Q.
    Powiązania:
    https://bibliotekanauki.pl/articles/259519.pdf
    Data publikacji:
    2017
    Wydawca:
    Politechnika Gdańska. Wydział Inżynierii Mechanicznej i Okrętownictwa
    Tematy:
    steel tube umbilical
    fatigue life
    contact and friction
    non-linear local stress
    Opis:
    The fatigue problem induced by wave loads and floater movements of dynamic umbilicals in deepwater is studied. The prediction method of the fatigue life is investigated by considering no-linear local stress due to contact and friction between components of the umbilical. A case study of a dynamic umbilical for 1500 meters depth in South China Sea was presented. The results showed that the more accurate result of the fatigue life was calculated by considering non-linear local stress. The fatigue life by considering local stress with the no-slip assumption was conservative. An obviously longer fatigue life was obtained by considering local stress with the full-slip assumption.
    Źródło:
    Polish Maritime Research; 2017, S 2; 154-163
    1233-2585
    Pojawia się w:
    Polish Maritime Research
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Design of Acoustic Tubes Array and Application to Measuring Acoustic Loads in Supersonic Airflow
    Autorzy:
    Wei, L.
    Li, M.
    Fu, Q.
    Fan, Y.
    Yang, D.
    Powiązania:
    https://bibliotekanauki.pl/articles/176750.pdf
    Data publikacji:
    2014
    Wydawca:
    Polska Akademia Nauk. Czytelnia Czasopism PAN
    Tematy:
    acoustic load
    acoustic tubes array
    near field measurement
    supersonic airflow
    Opis:
    In the acoustic fatigue experiment for hypersonic vehicle in simulated harsh service environment on ground, acoustic loads on the surface of test pieces of the vehicle need to be measured. However, for the normal microphones without high temperature resistance ability, the near field sound measurement cannot be achieved. In this work, on the basis of previous researches, an acoustic tubes array is designed to achieve the near field measurement of acoustic loads on the surface of the test piece in the supersonic airflow with high temperature achieved by coherent jet oxygen lance. Firstly, the process of designing this acoustic tubes array is introduced. Secondly, the equality of phase differences at the front and at the end of the tubes is stated and proved using a phase differences test with an acoustic tubes array whose design is presented in this text; therefore, the phase differences of signals acquired by microphones can be directly applied to beamforming algorithm to determine the acoustic load source. Finally, using above mentioned acoustic tubes array, measurement of acoustic load, with and without a test piece in the supersonic airflow made by the coherent jet oxygen lance, is conducted respectively, and the measurements results are analyzed.
    Źródło:
    Archives of Acoustics; 2014, 39, 3; 395-402
    0137-5075
    Pojawia się w:
    Archives of Acoustics
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Effects of Neuromedin S on the proliferation of splenic lymphocytes and the cytokine secretion by pulmonary alveolar macrophages in pigs in vitro
    Autorzy:
    Lin, R.
    Wang, Q.
    Qi, B.
    Huang, Y.
    Yang, G.
    Powiązania:
    https://bibliotekanauki.pl/articles/30295.pdf
    Data publikacji:
    2016
    Wydawca:
    Polska Akademia Nauk. Czytelnia Czasopism PAN
    Opis:
    Neuromedin S (NMS), a 36-amino acid neuropeptide, has been found to be involved in the regulation of the endocrine activity. It has been also detected in immune tissues in mammals, what suggests that NMS may play an important role in the regulation of immune response. The aim of this study was to demonstrate the presence of NMS receptor 1 (NMU1R) and effect of NMS in pig splenic lymphocytes (SPLs) and pulmonary alveolar macrophages (PAMs). The presence of NMU1R in pig SPLs and PAMs was respectively confirmed by reverse transcription-polymerase chain reaction (RT-PCR), western blot analysis and immunocytochemical methods. Furthermore, SPL proliferation was analyzed using the 3-(4,5)-dimethyl-thiahiazo-(-2-yl)-3,5-di-phenytetrazoliumromide (MTT) method. Additionally, the secretion of interleukin (IL)-1β, IL-6 and tumor necrosis factor-α (TNF-α) in PAMs was all measured by enzyme-linked immunosorbent assay (ELISA) kits. In the present study, the results of RT-PCR and western blot analysis revealed that NMU1R mRNA and protein were both expressed in pig SPLs and PAMs, and the immunocytochemical investigations further revealed that the positive signal of NMU1R immunoreactivity was observed in plasma membranes of both SPLs and PAMs. In the in vitro study, we found that at concentrations of 0.001-1000 nM NMS alone or combined with lipopolysaccharide or phytohemagglutinin significantly increased SPL proliferation. Application of ELISA method showed that NMS could induce the secretion of the pro-inflammatory cytokines IL-1β, IL-6 and TNF-α in PAMs. These results suggest that NMS can act as a potently positive pro-inflammatory factor and immunomodulatory agent that affects the immune response of immune cells by combining with its receptor NMU1R.
    Źródło:
    Polish Journal of Veterinary Sciences; 2016, 19, 3
    1505-1773
    Pojawia się w:
    Polish Journal of Veterinary Sciences
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Relationship between flotation and Fe/Mn ratio of wolframite with benzohydroxamic acid and sodium oleate as collectors
    Autorzy:
    Yang, S.
    Feng, Q.
    Qiu, X.
    Gao, Y.
    Xie, Z.
    Powiązania:
    https://bibliotekanauki.pl/articles/109666.pdf
    Data publikacji:
    2014
    Wydawca:
    Politechnika Wrocławska. Oficyna Wydawnicza Politechniki Wrocławskiej
    Tematy:
    wolframite
    flotation
    collector mixture
    benzohydroxamic acid
    sodium oleate
    Opis:
    Several studies revealed that flotation of wolframite changes with different Fe/Mn ratios, but they did not reach a consensus. This relationship in the presence of benzohydroxamic acid (BHA) and sodium oleate (NaOl) as collectors was studied in this paper through comparison of probability distribution curve of wolframite with different Fe/Mn ratios between the raw ore and the flotation concentrate, the pure mineral flotation and solution chemistry of flotation. The results showed that wolframite with high Fe/Mn ratio showed higher flotation with BHA as a collector while the flotation behavior of wolframite was completely opposite with NaOl as a collector. Besides, the calculations of chemical equilibrium in the solution were plotted as ΔG°-pH graphs. The results revealed that the flotation of wolframite may be determined by the interaction between BHA and ferric(II) ion or between NaOl and manganous ion. From the perspective of collector mixture, the results also explain the high collecting capability of the BHA/NaOl collector mixture, which can be defined as “functional complementation”.
    Źródło:
    Physicochemical Problems of Mineral Processing; 2014, 50, 2; 747-758
    1643-1049
    2084-4735
    Pojawia się w:
    Physicochemical Problems of Mineral Processing
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    GP5 protein - based ELISA for the detection of PRRSV antibodies
    Autorzy:
    Wang, Y.
    Guo, J.
    Qiuao, S.
    Li, Q.
    Yang, J.
    Jin, Q.
    Zhang, G.
    Powiązania:
    https://bibliotekanauki.pl/articles/30629.pdf
    Data publikacji:
    2016
    Wydawca:
    Polska Akademia Nauk. Czytelnia Czasopism PAN
    Opis:
    Porcine reproductive and respiratory syndrome virus (PRRSV) is an important swine pathogen, causing huge economic losses each year worldwide. Immunization with vaccines containing the glycoprotein 5 (GP5) of PRRSV is the main measure to induce neutralizing antibodies and control the disease. Here, we developed a GP5 protein-based ELISA for detecting antibodies against PRRSV. The overall yield of purified GP5 in E. coli flask culture was more than 45 mg/L cell culture. Western blot and IFA indicated that the GP5 protein was highly immunogenic. After optimization and validation with IDEXX PRRS using 566 clinical sera, the DSN, DSP, and accuracy of GP5-ELISA were 81.39%, 75.96%, and 80.39%, respectively. Besides, GP5-ELISA is highly specific, showing no cross-reactions with sera against other important swine pathogens. Hence, GP5 is a good diagnostic antigen and the GP5 protein-based ELISA has the potential to be used in the field
    Źródło:
    Polish Journal of Veterinary Sciences; 2016, 19, 3
    1505-1773
    Pojawia się w:
    Polish Journal of Veterinary Sciences
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    In vitro cloning potential and phytochemical evaluations of aneuploid individuals produced from reciprocal crosses between diploid and triploid in Echinacea purpurea L.
    Autorzy:
    Li, Q.
    Jiang, W.
    Ren, Y.
    Chen, R.
    Li, X.
    Yang, Y.
    Wu, H.
    Powiązania:
    https://bibliotekanauki.pl/articles/58769.pdf
    Data publikacji:
    2017
    Wydawca:
    Polskie Towarzystwo Botaniczne
    Opis:
    Aneuploidy often presents large variations in morphology, physiology, biochemistry, and genetics owing to karyotypic imbalance. This study aimed to evaluate the efficacy of aneuploid breeding in Echinacea purpurea L, an important medicinal plant. Reciprocal crosses between diploid and triploid plants were performed to generate aneuploid plants. Cross with triploid as female parent resulted in increased production of aneuploid individuals (19 of 23; 82.61%), while using diploid as female parent yielded much higher percentage of diploid progenies (130 of 133; 97.74%). Each aneuploid had particular karyotypic characteristics compared to the parents. The proportions of median, submedian, and subterminal centromere location chromosomes in gross chromosomes among aneuploids and two parents showed large variations. Although aneuploids had relatively lower adventitious bud regeneration rates than their parents, almost half of them looked morphologically normal, with high survival rates when transplanted to ex vitro conditions. Among the bioactive compounds assessed, cichoric acid and chlorogenic acid contents were extremely encouraging. Most aneuploids had higher cichoric acid and chlorogenic acid contents than their parents. For example, A2 had the highest cichoric acid content of 21.98 mg/g dry weight, more than twice the values of diploid and triploid. Meanwhile, A21 had the highest chlorogenic acid content of 1.84 mg/g, approximately five times more than the parental values. Eleven superior aneuploid lines were successfully screened as breeding candidates. The present findings indicated E. purpurea is highly tolerant of karyotypic imbalance and aneuploid plants could serve as prospective breeding resources in E. purpurea.
    Źródło:
    Acta Societatis Botanicorum Poloniae; 2017, 86, 3
    0001-6977
    2083-9480
    Pojawia się w:
    Acta Societatis Botanicorum Poloniae
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Digital volume correlation in an environment with intensive salt-and-pepper noise and strong monotonic nonlinear distortion of light intensity
    Autorzy:
    Tu, P.
    Bai, Y.
    Xu, W.
    Dong, B.
    Ye, S.
    Yang, Q.
    Zhou, Y.
    Powiązania:
    https://bibliotekanauki.pl/articles/173712.pdf
    Data publikacji:
    2017
    Wydawca:
    Politechnika Wrocławska. Oficyna Wydawnicza Politechniki Wrocławskiej
    Tematy:
    digital volume correlation
    correlation function
    salt-and-pepper noise
    nonlinear effects
    Opis:
    Digital volume correlation is an image-based technique for internal 3D displacement and strain fields measurement or analysis widely used in the field of experimental mechanics. A widely used correlation function (criterion) of digital volume correlation is Pearson correlation function, which suffers from the problem of the acquired data being contaminated by salt-and-pepper noise and monotonic nonlinear distortion of the light intensity. In this work, a 3D correlation function called the Spearman correlation function is used to deal with those interferences. A numerical experiment shows that the performance of Spearman correlation function using integer-pixel registration in an environment with 10% salt-and-pepper noise is better than that of Spearman and Pearson correlation functions using sub-pixel registration in an environment with 1% salt-and-pepper noise. As the light intensity distortion is significant, the error of Pearson correlation function is considerable; meanwhile, the error of Spearman correlation function is small. In conclusion, Spearman correlation function is, in particular, practical and useful in digital volume correlation.
    Źródło:
    Optica Applicata; 2017, 47, 2; 209-223
    0078-5466
    1899-7015
    Pojawia się w:
    Optica Applicata
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Microstructure and Mechanical Properties of Duplex Structured Mg-Li-Zn-Y Alloys
    Autorzy:
    Su, J.
    Yang, Y.
    Fu, X.
    Ma, Q.
    Ren, F.
    Peng, X.
    Powiązania:
    https://bibliotekanauki.pl/articles/381787.pdf
    Data publikacji:
    2018
    Wydawca:
    Polska Akademia Nauk. Czytelnia Czasopism PAN
    Tematy:
    alloy Mg-Li-Zn-Y
    microstructure
    mechanical properties
    extruded
    stop Mg-Li-Zn-Y
    mikrostruktura
    właściwości mechaniczne
    wytłaczanie
    Opis:
    As-cast Mg-6Li-0.3Zn-0.6Y and Mg-6Li-1.2Zn-1.2Y (wt%) alloys were prepared and extruded at 260 ºC with an extrusion ratio of 25. The microstructure and mechanical behavior of as-cast and extruded alloys are reported and discussed. The results show that Mg-6Li-1.2Zn- 1.2Y alloy is composed of α-Mg, β-Li, and W-Mg3Zn3Y2 phases while Mg-6Li-0.3Zn-0.6Y alloy contains α-Mg, β-Li, W-Mg3Zn3Y2 phase and X-Mg12ZnY. After hot extrusion, the microstructure of specimens is refined and the average grains size of extruded alloys is 15 μm. Dynamic recrystallization occurs during the extrusion, leading to grain refinement of test alloys. Both the strength and elongation of test alloys are improved by extrusion. The extruded Mg-6Li-0.3Zn-0.6Y alloy possesses an ultimate strength of 225 MPa with an elongation of 18% while the strength and elongation of Mg-6Li-1.2Zn-1.2Y alloy are 206 MPa and 28%, respectively. The X-phase in Mg-6Li-0.3Zn- 0.6Y is beneficial to the improvement of strength, but will lead to the decrease of ductility.
    Źródło:
    Archives of Foundry Engineering; 2018, 18, 1; 181-185
    1897-3310
    2299-2944
    Pojawia się w:
    Archives of Foundry Engineering
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Measurement of the Heat of Reaction of Polytetrafluoroethylene/Aluminum Composites Based on Laser Initiation
    Autorzy:
    Li, S.
    Wu, Y.
    Lin, Q.
    Huang, C.
    Yang, S.
    Li, J.
    Powiązania:
    https://bibliotekanauki.pl/articles/358195.pdf
    Data publikacji:
    2017
    Wydawca:
    Sieć Badawcza Łukasiewicz - Instytut Przemysłu Organicznego
    Tematy:
    reactive materials
    PTFE/Al composites
    heat of reaction
    laser initiation
    Opis:
    Polytetrafluoroethylene/aluminum (PTFE/Al) composites are reactive materials which can release energy due to exothermic chemical reactions initiated under shock loading conditions. In order to accurately measure the potential maximum heat of reaction of PTFE/Al composites in an inert atmosphere, we propose in this paper a heat of reaction measurement system based on laser initiation. Our results show that the measurement system successfully initiates the chemical reaction between PTFE and Al in an argon atmosphere. The comparison between theoretical calculations and experimental data demonstrates that our measurement method is highly accurate and exhibits excellent consistency. Thus, the heat of reaction measurement system based on laser initiation is applicable for measuring the heat of reaction of PTFE/Al composites and also other complicated reactive materials.
    Źródło:
    Central European Journal of Energetic Materials; 2017, 14, 3; 534-546
    1733-7178
    Pojawia się w:
    Central European Journal of Energetic Materials
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Fatigue life prediction for Ni-based superalloy GH4169 considering machined surface roughness and residual stress effects
    Autorzy:
    Wu, Z. R.
    Wang, S. Q.
    Yang, X.
    Pan, L.
    Song, Y. D.
    Powiązania:
    https://bibliotekanauki.pl/articles/1839712.pdf
    Data publikacji:
    2021
    Wydawca:
    Polskie Towarzystwo Mechaniki Teoretycznej i Stosowanej
    Tematy:
    surface roughness
    residual stress
    fatigue crack
    life prediction
    GH4169
    Opis:
    Establishing a fatigue life prediction model considering the machined surface state is of great significance to improve fatigue life prediction accuracy. Fatigue tests with different machined surface states of GH4169 alloy were conducted firstly. The influence of surface state parameters on the fatigue life was analyzed. Then, the machined surface stress concentration factor and residual stress were introduced into the fatigue crack initiation and propagation models. Finally, the fatigue life prediction model of GH4169 material considering the surface states was established. The prediction results based on the proposed model are almost within a factor of two scatter band of the test results.
    Źródło:
    Journal of Theoretical and Applied Mechanics; 2021, 59, 2; 215-226
    1429-2955
    Pojawia się w:
    Journal of Theoretical and Applied Mechanics
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Generation and characterization of a monoclonal antibody against duck Tembusu virus envelope protein
    Autorzy:
    Han, K.
    Zhao, D.
    Liu, Y.
    Liu, Q.
    Huang, X.
    Yang, J.
    Bi, K.
    Xu, T.
    Li, Y.
    Powiązania:
    https://bibliotekanauki.pl/articles/31495.pdf
    Data publikacji:
    2016
    Wydawca:
    Polska Akademia Nauk. Czytelnia Czasopism PAN
    Opis:
    Duck Tembusu virus (DTMUV) is a newly emerging pathogenic flavivirus that has caused massive economic losses to the duck industry in China. Envelope (E) protein of DTMUV is an important structural protein, which is able to induce protective immune response in target animals and can be used as specific serological diagnosis tool. In this study, a novel monoclonal antibody, designated mAb 3E9, was generated against DTMUV E protein. It is positive in indirect ELISA against both His-E protein and the purified whole viral antigen. Also, this mAb showed positive reaction with DTMUV in Western blot and indirect immunofluorescence assay, and the isotype was IgG1. End-point neutralizing assay performed in BHK-21 cells revealed that the neutralization titer of 3E9 against DTMUV JS804 strain reached 1:50. Furthermore, functional studies revealed that 3E9 blocks infection of DTMUV at a step on viral attachment. The anti-E mAbs produced in the present work may be valuable in developing an antigen-capture ELISA test for antigen detection or a competitive ELISA test for antibody detection or therapeutic medicine for DTMUV in poultry.
    Źródło:
    Polish Journal of Veterinary Sciences; 2016, 19, 4
    1505-1773
    Pojawia się w:
    Polish Journal of Veterinary Sciences
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Selective reduction of PbSO4 to PbS with carbon and flotation treatment of synthetic galena
    Autorzy:
    Zheng, Y.-X.
    Liu, W.
    Qin, W.-Q.
    Han, J.-W.
    Yang, K.
    Luo, H.-L.
    Powiązania:
    https://bibliotekanauki.pl/articles/110717.pdf
    Data publikacji:
    2015
    Wydawca:
    Politechnika Wrocławska. Oficyna Wydawnicza Politechniki Wrocławskiej
    Tematy:
    wastes
    lead sulfate
    reduction
    carbon
    lead sulfide
    flotation
    Opis:
    In order to recover lead from the zinc leaching residues, a new technology involving selective reduction of lead sulfate to lead sulfide with carbon followed by flotation was investigated. The reduction thermodynamics of PbSO4 was discussed and the effects of molar ratio of C to PbSO4, reaction temperature and time were examined by thermogravimetry (TG) and XRD. Verification tests were further carried out to prove the conclusions of thermodynamic and TG analyses, and the transformation extent could reach 86.45% under the optimal roasting conditions. The prepared galena was then subjected to micro-flotation tests, and the highest lead recovery could reach up to 75.32%.
    Źródło:
    Physicochemical Problems of Mineral Processing; 2015, 51, 2; 535-546
    1643-1049
    2084-4735
    Pojawia się w:
    Physicochemical Problems of Mineral Processing
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Identification of a linear epitope in the capsid protein of goose astrovirus with monoclonal antibody
    Autorzy:
    Dai, G.
    Huang, X.
    Liu, Q.
    Li, Y.
    Zhang, L.
    Han, K.
    Yang, J.
    Liu, Y.
    Xue, F.
    Zhao, D.
    Powiązania:
    https://bibliotekanauki.pl/articles/16647377.pdf
    Data publikacji:
    2022
    Wydawca:
    Polska Akademia Nauk. Czasopisma i Monografie PAN
    Tematy:
    epitope
    goose astrovirus
    capsid protein
    monoclonal antibody
    Opis:
    Goose astrovirus (GoAstV) is a novel avastrovirus that typically causes gosling gout and results in 2 to 20% mortality. GoAstV capsid protein is the sole structural protein, which is responsible for viral attachment, assembly, maturation as well as eliciting host antibodies. However, the epitopes within capsid protein have not been well studied. In this study, a monoclonal antibody, named 1D7, was generated against GoAstV capsid protein by hybridoma technology. Western blot results showed that this MAb could react with recombinant capsid protein expressed in E. coli. Also, it recognized the precursor of capsid protein, VP90 and VP70, in GoAstV-infected cells. Besides, excellent specificity of MAb 1D7 was further demonstrated in indirect immunofluorescence assay and immunohistochemical analysis. Epitope mapping results revealed that MAb 1D7 recognized the epitope 33QKVY 36 within Cap protein. Sequence alignment indicated that 33QKVY 36 is a conserved epitope among the isolates of goose astrovirus type 2 (GoAstV-2), suggesting the potential for its use in GoAstV-2 specific diagnostic assay. These findings may provide some insight into a function of the GoAstV capsid protein and further contribute to the development of diagnostic methods for GoAstV infection.
    Źródło:
    Polish Journal of Veterinary Sciences; 2022, 25, 4; 579-587
    1505-1773
    Pojawia się w:
    Polish Journal of Veterinary Sciences
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Effect of experimental infections of various Tembusu virus strains isolated from geese, ducks and chickens on ducklings
    Autorzy:
    Xu, T.
    Huang, X.
    Li, Y.
    Liu, Q.
    Liu, X.
    Han, K.
    Liu, Y.
    Yang, J.
    Zhao, D.
    Bi, K.
    Sun, W.
    Powiązania:
    https://bibliotekanauki.pl/articles/2087719.pdf
    Data publikacji:
    2018
    Wydawca:
    Polska Akademia Nauk. Czytelnia Czasopism PAN
    Tematy:
    goose-derived
    duck-derived
    chicken-derived
    Tembusu virus
    pathogenicity
    Źródło:
    Polish Journal of Veterinary Sciences; 2018, 21, 2; 389-396
    1505-1773
    Pojawia się w:
    Polish Journal of Veterinary Sciences
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Effects of synthesis conditions on the synthesis of carbon nanofibers by ethanol catalytic combustion
    Wpływ warunków syntezy na syntezę nanowłókien węglowych metoda katalitycznego spalania etanolu
    Autorzy:
    Cheng, J.
    Zou, X. P.
    Zhu, G.
    Wang, M. F.
    Su, Y.
    Yang, G. Q.
    Lu, X. M.
    Powiązania:
    https://bibliotekanauki.pl/articles/354282.pdf
    Data publikacji:
    2012
    Wydawca:
    Polska Akademia Nauk. Czytelnia Czasopism PAN
    Tematy:
    synteza
    nanowłókna węglowe
    spalanie
    etanol
    carbon nanofibers
    ethanol catalytic combustion
    influence factors
    Opis:
    In this paper, the effects of position of substrates in flames, preparation time, stability of flames and catalyst precursors on the synthesis of carbon nanofibers (CNFs) by ethanol catalytic combustion (ECC) were investigated. For investigating the effects of these influence factors on the synthesis of CNFs, several sets of controlled experiments were performed, such as preparation experiments with different position of substrates in flames, different preparation time, stable and unstable flames, and different catalyst precursors. In our experiments, the catalyst precursors were iron nitrate, cobalt nitrate, nickel nitrate, and iron chloride, cobalt chloride, nickel chloride. The as-synthesized products were characterized by scanning electron microscopy (SEM), transmission electron microscopy (TEM), and Raman spectroscopy. Our results indicate that the optimal position of substrates in flames is more than 1cm and less than 2.5cm, the optimal preparation time is more than 5min and less than 30min for massive yield, stable flames would be tent to synthesize CNFs with mainly single-type morphology and could improve the graphitization of CNFs, and the catalyst precursors obviously have effects on the synthesis of CNFs.
    W niniejszej pracy zbadany został wpływ położenia substratów w płomieniach, czasu przygotowania, stabilności płomieni i prekursorów katalizatora na syntezę nanowłókien węglowych (CNFs) metodaą katalitycznego spalania etanolu (ECC). W celu zbadania wpływu tych czynników na syntezę nanowłókien węglowych przeprowadzono kilka zestawów kontrolowanych eksperymentów, przy różnej pozycji podłoży w płomieniach, różnym czasie przygotowania, płomieniach stabilnych i niestabilnych i przy użyciu różnych prekursorów katalizatora. W naszych eksperymentach jako prekursory katalizatora stosowano azotany: żelaza, kobaltu i niklu oraz chlorki żelaza, kobaltu i niklu. Tak otrzymane produkty syntezy scharakteryzowano za pomocą skaningowej mikroskopii elektronowej (SEM), transmisyjnej mikroskopii elektronowej (TEM) i spektroskopii Raman'a. Nasze wyniki wskazują, że optymalna pozycja podłoży w płomieniach to więcej niż 1 cm i mniej niż 2,5 cm, a optymalny czas przygotowania to więcej niż 5 min i mniej niż 30 min dla dużej wydajności. Stabilność płomieni będzie sprzyjać syntezie nanowłókien o morfologii jednego typu i może poprawić ich grafityzacje, a rodzaj prekursora katalizatora ma wpływ na syntezę nanowłókien.
    Źródło:
    Archives of Metallurgy and Materials; 2012, 57, 3; 745-751
    1733-3490
    Pojawia się w:
    Archives of Metallurgy and Materials
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Dispersion-compensating en/decoder for a time-spreading/wavelength-hopping optical code-division multiplexing (OCDM) system
    Autorzy:
    Zheng, J.
    Wang, R.
    Pu, T.
    Lu, L.
    Fang, T.
    Su, Y.
    Li, L.
    Yang, Q.
    Chen, X.
    Powiązania:
    https://bibliotekanauki.pl/articles/174365.pdf
    Data publikacji:
    2013
    Wydawca:
    Politechnika Wrocławska. Oficyna Wydawnicza Politechniki Wrocławskiej
    Tematy:
    optical code-division multiplexing (OCDM)
    fiber Bragg grating (FBG)
    dispersion
    Opis:
    A novel dispersion-compensating fiber Bragg grating (FBG)-based en/decoder is proposed to compensate both the out-band and in-band dispersion in a time-spreading/wavelength-hopping (TS/WH) optical code-division multiplexing (OCDM) system. The experimental realization of such en/decoders only needs a uniform-pitch phase mask and a sub-micrometer precision moving stage. Such an en/decoder pair with the ability of compensating the dispersion of transmission in 20-km single mode fiber (SMF) is simulated and experimentally fabricated. Both the simulation and experimental results show that the decoded pulse can be recovered without any distortion owing to the elimination of dispersion.
    Źródło:
    Optica Applicata; 2013, 43, 3; 485-495
    0078-5466
    1899-7015
    Pojawia się w:
    Optica Applicata
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Rapid screening of monoclonal antibodies against porcine circovirus type 2 using colloidal gold-based paper test
    Autorzy:
    Jin, Q.Y.
    Feng, L.L.
    Wang, Y.B.
    Li, P.
    Yang, J.F.
    Teng, M.
    Chai, S.J.
    Xing, G.X.
    Zhang, G.P.
    Powiązania:
    https://bibliotekanauki.pl/articles/16539073.pdf
    Data publikacji:
    2022
    Wydawca:
    Polska Akademia Nauk. Czasopisma i Monografie PAN
    Tematy:
    porcine circovirus type 2 (PCV2)
    screening of MAbs
    paper test
    Opis:
    A proof of concept for using paper test as a suitable method in the production of monoclonal antibodies (MAbs) is reported. The paper test which detects antibodies against porcine circovirus type 2 (PCV2) using colloidal gold-labelled capsid protein as the antigen probe was applied exclusively in the screening of anti-PCV2 MAbs. It allowed the detection of 118 single cell clones within 30 min using naked eyes. MAbs with specific binding to authentic epitopes on the virus were selected using a blocking strategy in which the antibody was pre-incubated with PCV2 viral sample before applying to the test paper. Five hybridomas secreting MAbs against the capsid protein were obtained, with only three of them capable of binding to PCV2. The results were validated and confirmed using enzyme-linked immunosorbent assay and immunofluorescence assay. The paper test is simple, rapid, and independent on professional technicians and proves to be an excellent approach for the screening of MAbs against specific targets.
    Źródło:
    Polish Journal of Veterinary Sciences; 2022, 25, 1; 27-34
    1505-1773
    Pojawia się w:
    Polish Journal of Veterinary Sciences
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    The unsteadiness of tip clearance flow and its effect to stability of transonic axial compressor
    Nieustaloność przepływu w szczelinie wierzchołkowej i jej wpływ na stabilność pracy przydźwiękowej sprężarki osiowej
    Autorzy:
    Hu, J. F.
    Zhu, X. C.
    Ou-Yang, H.
    Tian, J.
    Wu, Y. D.
    Qiang, X. Q.
    Zhao, G.
    Du, Z. H.
    Powiązania:
    https://bibliotekanauki.pl/articles/281445.pdf
    Data publikacji:
    2013
    Wydawca:
    Polskie Towarzystwo Mechaniki Teoretycznej i Stosowanej
    Tematy:
    tip leakage flow
    compressor
    stability
    Opis:
    The steady and unsteady RANS simulations of a transonic compressor rotor (NASA rotor 37) are performed to investigate the tip clearance flow characteristic and correlations between tip leakage flow and compressor stability. For steady simulations, the results are compared with the aerodynamic probe and laser anemometer data. The speed lines and span-wise aerodynamic parameters agree well with the experimental data. On the other hand, the tip clearance flow of unsteady simulations are analysed clearly at a near stall condition. The results show that there is a mass flow rate boundary. The tip clearance flow oscillates substantially with a frequency about 50% BPF when the mass is less than that, which is caused by tip clearance flow, shock, and the interaction between them and the oncoming flow. The interface between the oncoming flow and clearance flow shifts forward, and the tip clearance flow may spill over into the adjacent blade passage as the mass flow decreases, which may results in the spike stall inception.
    W pracy przedstawiono symulacje stanu ustalonego i nieustalonego przydźwiękowej sprężarki osiowej (turbina NASA 37) z zastosowaniem metody Reynoldsa uśredniania równań Naviera-Stokesa (RANS) w celu zbadania charakterystyki przepływu w szczelinie wierzchołkowej oraz określenia zależności pomiędzy stratami związanymi z upływem w tej szczelinie a stabilnością pracy sprężarki. Wyniki symulacji stanu ustalonego porównano z danymi doświadczalnymi uzyskanymi za pomocą sondy aerodynamicznej i laserowego wiatromierza. Wyznaczone wzdłuż rozpiętości sprężarki linie prędkości przepływu i jego parametry aerodynamiczne okazały się zgodne z danymi doświadczalnymi. W przypadku symulacji stanu nieustalonego, analizę przepływu w szczelinie wierzchołkowej przeprowadzono dla warunków bliskich oderwania, tj. utraty wydajności sprężarki, wyznaczając graniczny wydatek przepływu dla takiej sytuacji. Poniżej tej granicznej wartości, zmiany przepływu w szczelinie oscylują z częstotliwością sięgającą 50% częstotliwości przejścia łopatek (tzw. BPF), co jest konsekwencją interakcji wywołanej zderzeniem przepływu z falą napływową w szczelinie wierzchołkowej. Powierzchnia tej interakcji przesuwa się do przodu, a sam przepływ może rozpaść się na fragmenty znajdujące ujście w kanałach przyległych łopatek. Zjawisko to zachodzi przy malejącym wydatku, a to z kolei może indukować oderwanie przepływu skokowymi zmianami mocy współpracującego silnika.
    Źródło:
    Journal of Theoretical and Applied Mechanics; 2013, 51, 2; 431-438
    1429-2955
    Pojawia się w:
    Journal of Theoretical and Applied Mechanics
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Comprehensive surveillance of the antibody response to Borrelia burgdorferi s.l. in small ruminants in China
    Autorzy:
    Yang, J.
    Liu, Z.
    Guan, G.
    Li, Y.
    Chen, Z.
    Ma, M.
    Liu, A.
    Ren, Q.
    Wang, J.
    Luo, J.
    Yin, H.
    Powiązania:
    https://bibliotekanauki.pl/articles/49708.pdf
    Data publikacji:
    2015
    Wydawca:
    Instytut Medycyny Wsi
    Źródło:
    Annals of Agricultural and Environmental Medicine; 2015, 22, 2
    1232-1966
    Pojawia się w:
    Annals of Agricultural and Environmental Medicine
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    The Microstructural Evolution in HMX Based Plastic-bonded Explosive During Heating and Cooling Process: an in situ Small-angle Scattering Study
    Autorzy:
    Yan, G.
    Tian, Q.
    Liu, J.
    Fan, Z.
    Sun, G.
    Zhang, C.
    Wang, Y.
    Chen, B.
    Gong, J.
    Zhou, X.
    Yang, Z.
    Nie, F.
    Li, J.
    Li, X.
    Powiązania:
    https://bibliotekanauki.pl/articles/358618.pdf
    Data publikacji:
    2016
    Wydawca:
    Sieć Badawcza Łukasiewicz - Instytut Przemysłu Organicznego
    Tematy:
    SANS
    SAXS
    HMX-PBX
    thermal damages
    phase transition
    Opis:
    The thermal damage in octahydro-1,3,5,7-tetranitro-1,3,5,7-tetrazocine (HMX) based plastic-bonded explosive (PBX) was investigated using in situ small-angle neutron and X-ray scattering techniques. The microstructural evolution was quantitatively characterized by the model fitting parameters of total interfacial surface area (Sv) and void volume distribution. The Sv of HMX-PBX decreased markedly above 100 °C, indicating the movement of binder into the voids. After subsequent cooling to room temperature, the scattering intensity increased significantly with increasing storage time, and a new population of voids with average diameter of 20 nm was observed, accompanied by the gradual phase transition of HMX from δ- to β-phase. The experimental results implied that serious damage within the HMX-PBX was developed during storage after heating.
    Źródło:
    Central European Journal of Energetic Materials; 2016, 13, 4; 916-926
    1733-7178
    Pojawia się w:
    Central European Journal of Energetic Materials
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
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