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    Wyświetlanie 1-3 z 3
    Tytuł:
    Cloning, purification and enzymatic characterization of recombinant human superoxide dismutase 1 (hSOD1) expressed in Escherichia coli
    Autorzy:
    Lin, Feng
    Yan, Dandan
    Chen, Yawen
    E, Fletcher
    Shi, Haifeng
    Han, Bangxing
    Zhou, Yang
    Powiązania:
    https://bibliotekanauki.pl/articles/1038395.pdf
    Data publikacji:
    2018
    Wydawca:
    Polskie Towarzystwo Biochemiczne
    Tematy:
    superoxide dismutase 1
    Escherichia coli
    soluble expression
    metal ions
    catalytic activity
    Opis:
    Superoxide dismutase 1 (SOD1) is a metalloenzyme that catalyzes the disproportionation of superoxide into molecular oxygen and hydrogen peroxide. In this study, the human SOD1 (hSOD1) gene was cloned, expressed and purified. The hSOD1 gene was amplified from a pool of Bxpc3 cell cDNAs by PCR and cloned into expression vector pET-28a (+). The recombinant soluble hSOD1 was expressed in E. coli BL21 (DE3) at 37°C and purified using nickel column affinity chromatography. Soluble hSOD1 was produced with a yield of 5.9 μg/mL medium. As metal ions can have a certain influence on protein structure and activity, we researched the influences of different concentrations of Cu2+ and Zn2+ on hSOD1 activity at induction and the time of activity detection. The results implied that Cu2+ and Zn2+ do not enhance SOD1 expression and solubility; they can, however, improve the catalytic activity at induction. Meanwhile, Cu2+ and Zn2+ also enhanced the enzyme activity at the time of detection. Furthermore, most other bivalent cations had the potential to replace Zn2+ and Cu2+, and also improved enzyme activity at the time of detection.
    Źródło:
    Acta Biochimica Polonica; 2018, 65, 2; 235-240
    0001-527X
    Pojawia się w:
    Acta Biochimica Polonica
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Understanding the urbanization impacts of high-speed rail in China
    Autorzy:
    Perl, Anthony
    Deng, Taotao
    Correa, Leandro
    Wang, Dandan
    Yan, Yulin
    Powiązania:
    https://bibliotekanauki.pl/articles/1832929.pdf
    Data publikacji:
    2021
    Wydawca:
    Polska Akademia Nauk. Czytelnia Czasopism PAN
    Tematy:
    High-Speed Rail
    high-speed rail's impacts
    China
    urbanization
    industry
    population
    Kolej Dużych Prędkości
    wpływ kolei dużych prędkości
    Chiny
    urbanizacja
    przemysł
    Opis:
    Advances in transport technology have been shown to play a vital role in urban development over millennia. From the engineering and pavement innovations of the Roman road network to the aerospace breakthroughs that enabled jet aircraft, cities have been reshaped by the mobility changes resulting from new designs for moving people and goods. This article explores the urbanization impacts of High-Speed Rail’s introduction in China, which has built the world’s largest High-Speed Rail network in record time. Since High-Speed Rail was launched in Japan in 1964, this technology has worked to reshape intercity travel as a revolutionary transportation alternative. High-Speed Rail has developed steadily across Japan, France, Germany, Italy, Switzerland during the 1970s and 1980s. It expanded to Russia, Spain, the United Kingdom, and Sweden in the 1990s. In the 21st century, China began developing High-Speed Rail on an unprecedented scale, and now has a national network that is longer than the totality of the rest of the world’s High-Speed Rail operations combined. China’s High-Speed Rail operation is exerting a transformative influence on urban form and function. This article synthesizes secondary research results to analyse the impacts of HSR on urbanization. These effects include population redistribution, urban spatial expansion and industrial development. We offer a typology that considers the urban effects of High-Speed Rail at three spatial levels: the station area, the urban jurisdiction, and the regional agglomeration. When organized through our typology, research findings demonstrate that High-Speed Rail influences urban population size, urban spatial layout and industrial development by changing the accessibility of cities. We highlight the processes by which High-Speed Rail ultimately affects the urbanization process for people, land use, and industrial development. However, High-Speed Rail’s impacts on urbanization are not always positive. While leveraging the development opportunity enabled by High-Speed Rail, governments around the world should also avoid potential negative impacts by drawing lessons from the experience of High-Speed Rail’s rapid deployment in China.
    Źródło:
    Archives of Transport; 2021, 58, 2; 21-34
    0866-9546
    2300-8830
    Pojawia się w:
    Archives of Transport
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    A new scheme of the environmental classification standards for the bronze cultural relic’s preservation in museums
    Autorzy:
    Li, Dandan
    Zhou, Hao
    Xu, Fangyuan
    Yan, Ying
    Wu, Laiming
    Cai, Lankun
    Powiązania:
    https://bibliotekanauki.pl/articles/28407393.pdf
    Data publikacji:
    2023
    Wydawca:
    Politechnika Wrocławska. Oficyna Wydawnicza Politechniki Wrocławskiej
    Tematy:
    QCM
    temperature
    humidity
    indoor exposure
    cultural relics
    mikrowaga kwarcowa
    temperatura
    wilgotność
    ekspozyja w pomieszczeniach
    zabytki kultury
    Opis:
    The environmental risk classification of the metal relics is usually determined by the corrosion rate of the metal but it is difficult to monitor the deterioration of the metal relics directly. A strong relationship exists between indoor exposure, the air quality classification of atmospheric corrosion, and the actual deterioration of metal relics. The copper-silver hanging plate method requires a long period of environmental exposure and has certain hysteresis, thus reflecting the current environmental quality of the museum in real time poses some difficulties. However, the application of the environmental reactivity monitor (ERMs) based on the piezoelectric effect can solve the above problems. The invented quartz crystal microbalance (QCM) reactivity monitoring device is applied to study the influence of temperature and humidity on the corrosion of the bronze-simulated materials and the relationship between the corrosion depth rate of the bronze-simulated materials and the frequency change of the crystal oscillator. Then, the recommended classification range of temperature and humidity and the airquality classification standards for the preservation environment of the bronze cultural relics in museums are proposed.
    Źródło:
    Environment Protection Engineering; 2023, 49, 4; 45--59
    0324-8828
    Pojawia się w:
    Environment Protection Engineering
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
      Wyświetlanie 1-3 z 3

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