- Tytuł:
- In vitro inhibition of topoisomerase IIα by reduced glutathione
- Autorzy:
-
Delwar, Zahid
Vita, Marina
Siden, Åke
Cruz, Mabel
Yakisich, Juan - Powiązania:
- https://bibliotekanauki.pl/articles/1039933.pdf
- Data publikacji:
- 2011
- Wydawca:
- Polskie Towarzystwo Biochemiczne
- Tematy:
-
DNA topoisomerases
glutathione
redox regulation - Opis:
- In most cells, the major intracellular redox buffer is glutathione (GSH) and its disulfide-oxidized (GSSG) form. The GSH/GSSG system maintains the intracellular redox balance and the essential thiol status of proteins by thiol disulfide exchange. Topoisomerases are thiol proteins and are a target of thiol-reactive substances. In this study, the inhibitory effect of physiological concentration of GSH and GSSG on topoisomerase IIα activity in vitro was investigated. GSH (0-10 mM) inhibited topoisomerase IIα in a concentration-dependent manner while GSSG (1-100 µM) had no significant effect. These findings suggest that the GSH/GSSG system could have a potential in vivo role in regulating topoisomerase IIα activity.
- Źródło:
-
Acta Biochimica Polonica; 2011, 58, 2; 265-267
0001-527X - Pojawia się w:
- Acta Biochimica Polonica
- Dostawca treści:
- Biblioteka Nauki
Artykuł