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Wyświetlanie 1-15 z 17
Tytuł:
Airflow Characteristics during the Rotor Spun Composite Yarn Spinning Process
Charakterystyka przepływu powietrza podczas przędzenia przędzy kompozytowej
Autorzy:
Yang, R.
Gao, W.
Xue, Y.
Powiązania:
https://bibliotekanauki.pl/articles/232164.pdf
Data publikacji:
2017
Wydawca:
Sieć Badawcza Łukasiewicz - Instytut Biopolimerów i Włókien Chemicznych
Tematy:
rotor-spun composite yarn
simulation
airflow
performance
przędza kompozytowa
symulacja
przepływ powietrza
Opis:
Rotor spun composite yarn shows compound performances when combined with staple fibres and filaments, such as excellent hand feeling as well as extreme elasticity and strength. Air characteristics including pressure and speed are critical factors of the rotor spun composite yarn spinning process. In this paper, air flow characteristics in a rotor composite yarn spinning unit are simulated and analysed by Ansys, and then verified by experiments. The results show that with the same spinning conditions, static pressure within the filament guide tube is lowest: -9 kPa and in rotor around -5 kPa. The speed of the airstream accelerates from the transfer channel inlet to the outlet, and reaches the largest value of 386 m/s at the outlet. As the rotor speed increases, the airflow velocity increases; the static pressure decreases; the breaking strength and CV of the composite yarn increase, and the breaking elongation and hairiness decrease according to the experiment results.
Przędza kompozytowa charakteryzuje się doskonałym chwytem, a także wyjątkową elastycznością i wytrzymałością. Charakterystyka przepływu powietrza, w tym ciśnienie i prędkość, są czynnikami decydującymi o przędzeniu przędzy kompozytowej na przędzarce rotorowej. W artykule przeprowadzono stymulację i analizę charakterystyk przepływu powietrza, a następnie wyniki zweryfikowano eksperymentalnie. Prędkość strumienia powietrza przyspiesza od wlotu kanału przenoszenia do wylotu i osiąga największą wartość 386 m/s na wylocie. Gdy prędkość wirnika wzrasta, prędkość przepływu powietrza również wzrasta, a ciśnienie statyczne maleje, natomiast wytrzymałość na zerwanie przędzy kompozytowej wzrasta.
Źródło:
Fibres & Textiles in Eastern Europe; 2017, 5 (125); 13-17
1230-3666
2300-7354
Pojawia się w:
Fibres & Textiles in Eastern Europe
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
A Conceptual Framework for Business Model Innovation: The Case of Electric Vehicles in China
Koncepcyjne ramy dla modelowych rozwiązań biznesowych: przypadek samochodów elektrycznych w Chinach
Autorzy:
Shao, L.
Xue, Y.
You, J.
Powiązania:
https://bibliotekanauki.pl/articles/371763.pdf
Data publikacji:
2014
Wydawca:
Polska Akademia Nauk. Komitet Człowiek i Środowisko PAN
Tematy:
business model innovation
electric vehicles
“3-7” structure system
two-phase conceptual framework
value network
modelowa innowacja biznesowa
samochody elektryczne
system strukturalny „3-7”
dwufazowe ramy konceptualne
wartość sieci
Opis:
EVs (electric vehicle), as sustainable technologies, hold the potential to achieve the sustainability of the transport system and challenge the prevailing business models of internal combustion engines (ICEs). To unlock the dominant logic of ICEs and promote the diffusion of EVs, Business model (BM) innovation is necessary. However, BM innovation for EVs still faces many obstacles. This paper makes contribution theoretically and practically by constructing a “3-7” system and proposing a two-phase conceptual framework for BM innovation of EVs. The case of Shenzhen City, China is studied to illustrate how the “3-7” system and two-phase conceptual framework is applied. Based on the case study, this paper concludes two new barriers that are not highlighted by the previous literature and makes a number of suggestions to help address the issues found in BM innovation.
Samochody elektryczne (EVs – electric vehicles), jako technologie prośrodowiskowe mogą doprowadzić do zrównoważoności całego systemu transportowego, stanowiąc tym samym wyzwanie dla dominującego modelu biznesowego tradycyjnych pojazdów spalinowych. Aby przełamać monopol maszyn spalinowych i doprowadzić do upowszechnienia samochodów elektrycznych, konieczne jest opracowanie biznesowego modelu innowacji. Niestety, w przypadku samochodów elektrycznych nadal napotyka on na wiele przeszkód. W tym artykule proponujemy rozwiązania zarówno na płaszczyźnie teoretycznej, jak i praktycznej, proponując system „3-7” i składające się z dwóch faz ramy konceptualne. W celach zobrazowania tego systemu wybrano przypadek miasta Shenzen w Chinach. Na tej podstawie w dyskusji zidentyfikowano dwie nowe bariery, który nie były dotąd omawiane w literaturze i zaproponowano szereg nowych rozwiązań, które powinny przyczynić się do rozwiązania istniejących problemów.
Źródło:
Problemy Ekorozwoju; 2014, 9, 2; 27-37
1895-6912
Pojawia się w:
Problemy Ekorozwoju
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Abnormal prediction of dense crowd videos by a purpose-driven lattice Boltzmann model
Autorzy:
Xue, Y.
Liu, P.
Tao, Y.
Tang, X.
Powiązania:
https://bibliotekanauki.pl/articles/329703.pdf
Data publikacji:
2017
Wydawca:
Uniwersytet Zielonogórski. Oficyna Wydawnicza
Tematy:
video surveillance
crowd analysis
abnormal events
lattice Boltzmann model
purpose driven strategy
monitoring wizyjny
analiza tłumu
zdarzenie nieprawidłowe
Opis:
In the field of intelligent crowd video analysis, the prediction of abnormal events in dense crowds is a well-known and challenging problem. By analysing crowd particle collisions and characteristics of individuals in a crowd to follow the general trend of motion, a purpose-driven lattice Boltzmann model (LBM) is proposed. The collision effect in the proposed method is measured according to the variation in crowd particle numbers in the image nodes; characteristics of the crowd following a general trend are incorporated by adjusting the particle directions. The model predicts dense crowd abnormal events in different intervals through iterations of simultaneous streaming and collision steps. Few initial frames of a video are needed to initialize the proposed model and no training procedure is required. Experimental results show that our purpose-driven LBM performs better than most state-of-the-art methods.
Źródło:
International Journal of Applied Mathematics and Computer Science; 2017, 27, 1; 181-194
1641-876X
2083-8492
Pojawia się w:
International Journal of Applied Mathematics and Computer Science
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Knitted silk mesh-like scaffold incorporated with sponge-like regenerated silk fibroin/collagen I and seeded with mesenchymal stem cells for repairing Achilles tendon in rabbits
Autorzy:
Tang, L.
Yang, Y.
Li, Y.
Yang, G.
Luo, T.
Xue, Y.
Zhang, W.
Powiązania:
https://bibliotekanauki.pl/articles/306523.pdf
Data publikacji:
2018
Wydawca:
Politechnika Wrocławska. Oficyna Wydawnicza Politechniki Wrocławskiej
Tematy:
ścięgno Achillesa
kolagen
komórki macierzyste
szpik kostny
Bombyx mori silk
regenerated silk fibroin
collagen I
bone marrow-derived mesenchymal stem cells
weft-knit
Achilles tendon action
Opis:
A scaffold knit with natural sericin-free silk fibroin fiber possesses desirable mechanical properties, biocompatibility, ease of fabrication, and slow degradability. However, regenerated silk fibroin degrades faster than natural silk. In this study, natural silk fibroin fiber mesh-like scaffolds were prepared by a weft-knitting method and the pores were filled with sponge-like regenerated silk fibroin-collagen I. The microporous sponge and mesh-like scaffolds were fused to achieve gradient degradation of the scaffolds, and rabbit bone marrow mesenchymal stem cells (BMSCs) were seeded onto the scaffolds to form scaffold–BMSCs composites. The composites were implanted into gap defects made in the rabbit Achilles tendon. Twenty weeks after implantation, histological observation showed that tendon-like tissue had formed, collagen I mRNA was expressed, abundant collagen was generated, and that there was no obvious degradation of silk. The maximum load of the neo-Achilles tendon was 62.14% that of the natural Achilles tendon. These outcomes were superior to those obtained in the group implanted with a scaffold without BMSCs. These findings suggest the feasibility of constructing tissue-engineered tendons using weft-knitted silk scaffolds incorporated with sponge-like regenerated silk fibroin/collagen I and seeded with BMSCs, and show potential of the scaffold–BMSCs composites to repair Achilles tendon defects.
Źródło:
Acta of Bioengineering and Biomechanics; 2018, 20, 3; 77-87
1509-409X
2450-6303
Pojawia się w:
Acta of Bioengineering and Biomechanics
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
New information on sexual dimorphism and allometric growth in Keichousaurus hui, a pachypleurosaur from the Middle Triassic of Guizhou, South China
Autorzy:
Xue, Y.
Jiang, D.
Motani, R.
Rieppel, O.
Sun, Y.
Sun, Z.
Ji, C.
Yang, P.
Powiązania:
https://bibliotekanauki.pl/articles/19926.pdf
Data publikacji:
2015
Wydawca:
Polska Akademia Nauk. Instytut Paleobiologii PAN
Opis:
Keichousaurus hui is a small pachypleurosaur (Reptilia: Sauropterygia) from the Triassic of China. Many specimens of various growth stages are known, making them ideal for ontogenetic research. We report 22 new specimens from the Middle Triassic of Xingyi (Guizhou, south China), and combined their skeletal measurements with those from 85 published specimens to analyze the ontogenetic trajectory of sexual dimorphism. An Exploratory Factor Analysis suggests that the largest factors behind morphological disparity within the species are body size followed by gender. Sexual dimorphism is most clearly reflected in selected skeletal ratios that are more pronounced in males than in females. We found that the relative length of femur to body size was useful in gender identification, in addition to three ratios that are traditionally used, namely a distal expansion of the humerus relative to its shaft, humerus length relative to body size, and humerus length relative to femur length. Two distinctive patterns exist in allometric changes of these four ratios. The distal expansion of the humerus is exceptional in that it is equally pronounced in juvenile and adult males and therefore must have been fully established during embryonic growth. The other three features are not pronounced at birth size and subsequently become pronounced during postembryonic growth. However, males and females already show different growth trajectories at birth size even in these three. Therefore, the fate of sexually dimorphic features seems to have already been set during embryonic growth in K. hui.
Źródło:
Acta Palaeontologica Polonica; 2015, 60, 3
0567-7920
Pojawia się w:
Acta Palaeontologica Polonica
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Synthesis and Characterization of a High Energy Combustion Agent (BHN) and Its Effects on the Combustion Properties of Fuel Rich Solid Rocket Propellants
Autorzy:
Pang, W.-Q.
Zhao, F.-Q.
Xue, Y.-N.
Xu, H.-X.
Fan, X.-Z.
Xie, W.-X.
Zhang, W.
Lv, J.
Deluca, L. T.
Powiązania:
https://bibliotekanauki.pl/articles/358474.pdf
Data publikacji:
2015
Wydawca:
Sieć Badawcza Łukasiewicz - Instytut Przemysłu Organicznego
Tematy:
fuel rich solid propellant
BHN
DSC
TG-DTG
burning rate
combustion properties
Opis:
A high energy combustion agent (tetraethylammonium decahydrodecaborate, BHN) was prepared by means of an ion exchange reaction (IER), and the prepared samples were characterized by the advanced diagnostic techniques of Scanning Electron Microscopy (SEM), X-ray diffraction (XRD), Thermogravimetric Analysis (TGA), and Differential Scanning Calorimetry (DSC) etc. The effects of BHN particles on the hazard and combustion properties of fuel rich solid propellants were investigated. The results showed that the BHN samples and fuel rich propellants containing BHN particles can be prepared successfully and solidified safely. The peak temperature of thermal decomposition and the heat of decomposition of the BHN samples prepared were 305.8 °C and 210.9 J•g-1 at a heating rate of 10 K•min-1, respectively. The burning rate and pressure exponent of fuel rich solid propellants decreases with increases in the fraction of BHN particles in the propellant formulation. Compared with the reference formulation (sample BP-1), the burning rate of the propellant with 10% mass fraction of BHN particles (sample BP-4) had decreased 30% at 3.0 MPa, and the pressure exponent had dropped from 0.44 to 0.41.
Źródło:
Central European Journal of Energetic Materials; 2015, 12, 3; 537-552
1733-7178
Pojawia się w:
Central European Journal of Energetic Materials
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
A fully coupled solid and fluid model for simulating coal and gas outburst with DEM and LBM
W pełni zintegrowany model oddziaływania pomiędzy cieczą a ciałem stałym do symulacji wyrzutów węgla i gazu metodą elementów dyskretnych i metodą siatkową Boltzmanna
Autorzy:
Wang, Y. C.
Xue, S.
Xie, J.
Powiązania:
https://bibliotekanauki.pl/articles/348657.pdf
Data publikacji:
2012
Wydawca:
Akademia Górniczo-Hutnicza im. Stanisława Staszica w Krakowie. Wydawnictwo AGH
Tematy:
wyrzut węgla i gazu
oddziaływanie ciecz-ciało stałe
metoda elementów dyskretnych
metoda siatkowa Boltzmanna
coal and gas outbursts
solid-fluid coupling
Discrete Element Method
lattice Boltzmann method
Opis:
In this paper, we present a fully coupled solid-fluid code which is developed to model the whole process of coal and gas outbursts. The Discrete Element Method is used to model the deformation and fracture of solid, while Lattice Boltzmann Method models fluid flow, including free flow and Darcy flow. These two methods are coupled in a two-way process: the solid part provides a moving boundary condition and transfers momentum to the fluid, and the fluid exerts a dragging force to the solid. Gas desorption occurs at solid-fluid boundary, and gas diffusion is implemented in the solid code where particles are assumed as porous material. Some preliminary simulations are carried out to validate the code.
W niniejszej publikacji prezentujemy w pełni zintegrowany kod oddziaływania pomiędzy cieczą a ciałem stałym, opracowany do modelowania całego procesu wyrzutów węgla i gazu. Metoda elementów dyskretnych stosowana jest do modelowania deformacji i pęknięcia ciała stałego, podczas gdy metoda siatkowa Boltzmanna - do modelowania przepływu cieczy, w tym przepływu swobodnego i przepływu zgodnie z prawem Darcy'ego. Te dwie metody połączone są w procesie dwukierunkowym: część stała zapewnia warunki ruchomej granicy rozdziału, przenosząc pęd do cieczy, a ciecz wywiera opór na ciele stałym. Desorpcja gazu występuje na granicy oddziaływania pomiędzy cieczą a ciałem stałym, a do rozproszenia gazu dochodzi w kodzie ciała stałego, gdzie cząsteczki traktowane są jako materiał porowaty. Prowadzone są wstępne symulacje w celu sprawdzenia poprawności kodu.
Źródło:
AGH Journal of Mining and Geoengineering; 2012, 36, 3; 377-384
1732-6702
Pojawia się w:
AGH Journal of Mining and Geoengineering
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Identification and expression of GA-related genes associated with in vitro micro-tubers formation in Pinellia ternata
Autorzy:
Xue, T.
Yue, E.
Chao, E.
Su, Y.
Zhang, W.
Zhu, Y.
Teng, J.
Xue, J.
Powiązania:
https://bibliotekanauki.pl/articles/11872893.pdf
Data publikacji:
2018
Wydawca:
Uniwersytet Przyrodniczy w Lublinie. Wydawnictwo Uniwersytetu Przyrodniczego w Lublinie
Opis:
Pinellia ternata is one of the most important herbs in traditional Chinese medicine. Recently, its yield is hard to meet the market demand, therefore the system of in vitro micro-tubers was developed for its propagation and endogenous GA was revealed probably function negatively during micro-tubers induction. However, the mechanism is still unknown. In this study, 70 mg/L chlormequat chloride (CCC) was successfully used to inhibit the endogenous GA content and promote the micro-tubers induction. Subsequently, suppression subtractive hybridization (SSH) was performed to identify GA-related genes associated with in vitro micro-tubers formation in P. ternata. The cDNAs of micro-tubers induced with and without CCC were used as the “tester”, and “driver”, respectively. SSH library sequencing yielded 300 expressed sequence tags (ESTs). Finally, 226 ESTs were retained after screening, 84 of which had no significant homology to any of previously identified genes and 39 of the remaining 142 ESTs represented singletons. Real-time quantitative RT-PCR analysis of the expression patterns showed that all 5 transcripts showed signal alteration during the process of in vitro micro-tubers formation. The sequences appeared to be highly homologous with 60S ribosomal protein, 26S ribosomal RNA gene, zinc transporter protein, 12kD storage protein and malate dehydrogenase, respectively. These results would facilitate the functional characterization of the GA-related genes associated with in vitro micro-tubers development and subsequent in vitro manipulation.
Źródło:
Acta Scientiarum Polonorum. Hortorum Cultus; 2018, 17, 6; 85-94
1644-0692
Pojawia się w:
Acta Scientiarum Polonorum. Hortorum Cultus
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Fluorescence Analysis as an Effective Method Used in Micro/Trace Explosive Detection
Autorzy:
Liu, Y.
Shu, Y.
Liu, Xue-yong.
Xiong, Y.
Zhong, Fa-chun.
Sun, Y.
Powiązania:
https://bibliotekanauki.pl/articles/358808.pdf
Data publikacji:
2009
Wydawca:
Sieć Badawcza Łukasiewicz - Instytut Przemysłu Organicznego
Tematy:
micro/trace explosive detection
fluorescence analysis
conjugated polymer
sensing materials
Opis:
There are many kinds of explosives, and their detection methods vary. Nitroaromatic compound is one of the composition of the explosives commonly used. The fuorescence will be quenched when they touch the fuorescent conjugated polymers. General methods for explosives detection have been summarized in this paper, in addition the application of novel fuorescence analysis technique in explosives detection has been introduced. Fluorescent conjugated polymer as chemical sensing material for explosive detection has been reviewed in detail, also a novel fuorescent sensing flm self-assembled by fuorescent small molecule pyrene and homogeneous fuoroimmunoassay have been presented briefy. The development of fuorescence analysis used in the area of determining explosives has been prospected.
Źródło:
Central European Journal of Energetic Materials; 2009, 6, 3-4; 303-311
1733-7178
Pojawia się w:
Central European Journal of Energetic Materials
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Experimental study on fracturing coal seams using CaO demolition materials to improve permeability
Autorzy:
Tang, Y.
Yuan, L.
Xue, J
Duan, C.
Powiązania:
https://bibliotekanauki.pl/articles/92132.pdf
Data publikacji:
2017
Wydawca:
Główny Instytut Górnictwa
Tematy:
soundless chemical demolition
coal seam
gas drainage
CaO
pokład węgla
odmetanowanie
Opis:
Currently, methane gas disasters in underground coal mines are a major problem which seriously threatens safe mining. This study employed soundless chemical demolition for fracturing coal seams with low permeability. Additionally, the fracturing theory and failure mechanisms of soundless chemical demolition agents were investigated. Materials such as CaO, naphthalene-based water reducer, sodium gluconate and silicate cement were used to prepare the novel soundless chemical demolition agent, whose optimum proportion was discovered to be 90:3:5:7 by carrying out orthogonal experiments. The innovative demolition agent cracked briquettes and the maximum width of cracks reached 16.33 mm, showing significant potential for improving the permeability of coal seams.
Źródło:
Journal of Sustainable Mining; 2017, 16, 2; 47-54
2300-1364
2300-3960
Pojawia się w:
Journal of Sustainable Mining
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Preparation of micro-encapsulated strawberry fragrance and its application in the aromatic wallpaper
Autorzy:
Xiao, Z.
Zhang, Y.
Zhu, G.
Niu, Y.
Xue, Z.
Zhu, J.
Powiązania:
https://bibliotekanauki.pl/articles/778533.pdf
Data publikacji:
2017
Wydawca:
Zachodniopomorski Uniwersytet Technologiczny w Szczecinie. Wydawnictwo Uczelniane ZUT w Szczecinie
Tematy:
micro-encapsulation
fragrance
wallpaper
controlled release
Opis:
Micro-encapsulated strawberry fragrance was successfully prepared with wall materials including maltodextrin, sodium octenylsuccinate and gum Arabic. The micro-capsule was added to wallpaper and aromatic wallpaper with strawberry characteristics was obtained. The particle distribution, surface morphology, chemical structure, thermal property and controlled release performance of micro-encapsulated fragrance and aromatic wallpaper were investigated using laser particle size analyzer, scanning electron microscopy (SEM), Fourier transform infrared spectroscopy (FT-TR), thermal gravity analysis (TGA) and chromatography-mass spectrometer (GC-MS). The results showed that the average diameter of micro-capsule was 2 μm and the particles mainly distributed in the tissues of wallpaper. The result of TGA showed that the micro-capsule had a good stability. Meanwhile, the aromatic wallpaper had strawberry aroma more than 3 months and took on excellent controlled release performance.
Źródło:
Polish Journal of Chemical Technology; 2017, 19, 1; 89-94
1509-8117
1899-4741
Pojawia się w:
Polish Journal of Chemical Technology
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Experimental and simulative study on accumulator function in the process of wave energy conversion
Autorzy:
Zhang, W.
Liu, Y.
Luo, H.
Xue, G.
Zhang, J.
Powiązania:
https://bibliotekanauki.pl/articles/259862.pdf
Data publikacji:
2016
Wydawca:
Politechnika Gdańska. Wydział Inżynierii Mechanicznej i Okrętownictwa
Tematy:
wave energy converter
AMESim simulation
accumulator
experimental verification
Opis:
In this paper, a floating-buoy wave energy converter using hydrostatic transmission system is studied. The entire work progress of wave energy power generation device is introduced, and the hydraulic transmission principles are emphasized through the simulation to verify the feasibility of design principle of hydraulic transmission system. The mathematical model of the accumulator is established and applied in the AMEsim simulation. The simulation results show that the accumulator plays an important role in the wave power hydraulic transmission system and that the correct configuration of accumulator parameters can improve the rapidity and stability of the system work. Experimental results are compared with the simulation results to validate the correctness of the simulation results. This would provide a valuable reference to the optimal design of wave power generation.
Źródło:
Polish Maritime Research; 2016, 3; 79-85
1233-2585
Pojawia się w:
Polish Maritime Research
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Proteomics profiles reveal the potential roles of proteins involved in chicken macrophages stimulated by Lipopolysaccharide
Autorzy:
Li, S.
Chen, Y.
Xue, W.
Wang, Q.
Huai, Z.
An, C.
Wang, Y.
Powiązania:
https://bibliotekanauki.pl/articles/16647586.pdf
Data publikacji:
2023
Wydawca:
Polska Akademia Nauk. Czasopisma i Monografie PAN
Tematy:
chicken macrophage (HD11)
immune response
lipopolysaccharide (LPS)
proteomics
Opis:
Lipopolysaccharide (LPS), a core part of gram-negative bacteria, is crucial for inducing an inflammatory response in living things. In the current study, we used LPS from Salmonella to stimulate chicken macrophages (HD11). Proteomics was used to investigate immune-related proteins and their roles further. Proteomics investigation revealed 31 differential expression proteins (DEPs) after 4 hours of LPS infection. 24 DEPs expressions were up-regulated, while seven were down-regulated. In this investigation, ten DEPs were mainly enriched in S. aureus infection, complement, and coagulation cascades, which were all implicated in the inflammatory response and clearance of foreign pathogens. Notably, complement C3 was shown to be up-regulated in all immune-related pathways, indicating that it is a potential protein in this study. This work contributes to a better understanding and clarification of the processes of Salmonella infection in chickens. It might bring up new possibilities for treating and breeding Salmonella-infected chickens.
Źródło:
Polish Journal of Veterinary Sciences; 2023, 26, 2; 265-274
1505-1773
Pojawia się w:
Polish Journal of Veterinary Sciences
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Modulation of virulence genes by the two-component system PhoP - PhoQ in avian pathogenic Escherichia coli
Autorzy:
Tu, J.
Huang, B.
Zhang, Y.
Xue, T.
Li, S.
Qi, K.
Powiązania:
https://bibliotekanauki.pl/articles/30418.pdf
Data publikacji:
2016
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Opis:
Avian pathogenic Escherichia coli (APEC) infections are a very important problem in the poultry industry. PhoP-PhoQ is a two-component system that regulates virulence genes in APEC. In this study, we constructed strains that lacked the PhoP or PhoQ genes to assess regulation of APEC pathogenicity by the PhoP-PhoQ two-component system. The PhoP mutant strain AE18, PhoQ mutant strain AE19, and PhoP/PhoQ mutant strain AE20 were constructed by the Red homologous recombination method. Swim plates were used to evaluate the motility of the APEC strains, viable bacteria counting was used to assess adhesion and invasion of chick embryo fibroblasts, and Real-Time PCR was used to measure mRNA expression of virulence genes. We first confirmed that AE18, AE19, and AE20 were successfully constructed from the wild-type AE17 strain. AE18, AE19, and AE20 showed significant decreases in motility of 70.97%, 83.87%, and 37.1%, respectively, in comparison with AE17. Moreover, in comparison with AE17, AE18, AE19, and AE20 showed significant decreases of 63.11%, 65.42%, and 30.26%, respectively, in CEF cell adhesion, and significant decreases of 59.83%, 57.82%, and 37.90%, respectively, in CEF cell invasion. In comparison with AE17, transcript levels of sodA, polA, and iss were significantly decreased in AE18, while transcript levels of fimC and iss were significantly decreased in AE19. Our results demonstrate that deletion of PhoP or PhoQ inhibits invasion and adhesion of APEC to CEF cells and significantly reduces APEC virulence by regulating transcription of virulence genes.
Źródło:
Polish Journal of Veterinary Sciences; 2016, 19, 1
1505-1773
Pojawia się w:
Polish Journal of Veterinary Sciences
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Optimal design and numerical simulation on fish-like flexible hydrofoil propeller
Autorzy:
Xue, G.
Liu, Y.
Zhang, M.
Zhang, W.
Zhang, J.
Luo, H.
Jia, R.
Powiązania:
https://bibliotekanauki.pl/articles/259984.pdf
Data publikacji:
2016
Wydawca:
Politechnika Gdańska. Wydział Inżynierii Mechanicznej i Okrętownictwa
Tematy:
bionics
flexible hydrofoil
phase angle
hydrodynamic coefficient
Opis:
Hydrofoil is widely used in underwater vehicle for the excellent hydrodynamic characteristics. Currently, researches are mostly about the rigid hydrofoil while the flexible hydrofoil, like the caudal fin, has not been studied adequately. In this paper, the fish was regarded as the bionic object. Then the kinematics model to describe the fish swimming was put forward. A fin-peduncle propulsion mechanism was designed based on the kinematics model to achieve the similar sine curve swimming model. The propulsion mechanism was optimized by Matlab to reduc the deviation between the output curve of the fin-peduncle propulsion mechanism and the ideal motion trajectory. Moreover, the motion phase angles among flexible articulations are optimized to reduce fluid resistance and improve propulsive efficiency. Finally, the fish-like hydrofoil oscillation is simulated by fluid-solid coupling method based on the Fluent. It was shown that the optimized flexible fish-like oscillation could generate the motion that follows the similar law of sine. The propulsive efficiency of oscillating hydrofoil propeller is much higher than that of the screw propeller, and the flexible oscillation has higher propulsive efficiency than the rigid oscillation without obvious fluid resistance increase.
Źródło:
Polish Maritime Research; 2016, 4; 59-66
1233-2585
Pojawia się w:
Polish Maritime Research
Dostawca treści:
Biblioteka Nauki
Artykuł
Wyświetlanie 1-15 z 17

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