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Tytuł:
3D Simulation of Warp Knitted Structures with a New Algorithm Based on NURBS
Symulacja 3D struktur dzianych z zastosowaniem algorytmu opartego na algorytmie NURBS
Autorzy:
Xu, H Y
Chen, N L
Jiang, J H
Jin, L X
Wang, Z X
Powiązania:
https://bibliotekanauki.pl/articles/232913.pdf
Data publikacji:
2015
Wydawca:
Sieć Badawcza Łukasiewicz - Instytut Biopolimerów i Włókien Chemicznych
Tematy:
warp knitted stitch
three-dimensional simulation
NURBS
joint of stitches
MATLAB
ścieg osnowy tkaniny
struktura dzianin
symulacja trójwymiarowy
algorytm NURBS
wspólne szwów
oprogramowanie Matlab
Opis:
In order to obtain a three-dimensional computer simulation of warp knitted structures with more flexibility and realism, a new algorithm using Matlab was developed by NURBS based on empirical geometrical loop models. With the principles of NURBS curves, once the values of data points are known, the control points with two coincidence points at the start and end points can be uncomplicatedly calculated by Matlab. Then the NURBS curve of a single typical stitch can be simulated flexibly by Matlab. A new typical stitch selected from two stitches simulated directly by the new method is redefined to improve the joint of neighboring stitches, and it is found that there are two types of redefined typical warp knitted stitches judged by whether the two under lap on the same side or not. Based on the redefined typical warp knitted stitch, two warp knitted structures are simulated regardless of the loop offset, and all the joints of stitches are smooth.
Przeprowadzono symulację komputerową struktur dzianin w wymiarze 3D, opracowując w tym celu nową procedurę opartą na algorytmie NURBS. Do tego celu wykorzystano oprogramowanie MATLAB. Symulację 3D przeprowadzono w oparciu o procedury numeryczne umożliwiające wykreślenie przestrzennych figur geometrycznych opisujących strukturę dzianin. Symulowano kształt oczek zamkniętych i otwartych podstawowych splotów kolumienkowych trykotu, sukna i aksamitu. W oparciu o 8 punktów referencyjnych zorientowano konfigurację pętli oczka i łącznika w postaci przestrzennej linii eliptycznej. Do zapisu struktury splotu kształtu oczek wykorzystywano aparat matematyczny w postaci macierzy.
Źródło:
Fibres & Textiles in Eastern Europe; 2015, 1 (109); 57-60
1230-3666
2300-7354
Pojawia się w:
Fibres & Textiles in Eastern Europe
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
A new troodontid theropod from the Late Cretaceous of central China, and the radiation of Asian troodontids
Autorzy:
Lu, J
Xu, L.
Liu, Y.
Zhang, X.
Jia, S.
Ji, Q.
Powiązania:
https://bibliotekanauki.pl/articles/20912.pdf
Data publikacji:
2010
Wydawca:
Polska Akademia Nauk. Instytut Paleobiologii PAN
Tematy:
new theropod
Late Cretaceous
Central China
Asian troodontid
troodontid
Theropoda
Troodontidae
Xixiasaurus
Cretaceous
Henan province
China
paleontology
Opis:
A new troodontid dinosaur, Xixiasaurus henanensis gen. et sp. nov., from the Upper Cretaceous Majiacun Formation of the Xixia Basin, Henan Province, is erected, based on a partial skull. It is characterized by bearing 22 maxillary teeth, a distinct opening on the lateral surface of the base of nasal process of the premaxilla, the rostral end of the upper jaw forming a tapered U−shape, and the mandibular symphyseal region slightly inflected medially. Xixiasaurus is most closely related to the Mongolian Byronosaurus among troodontids. Byronosaurus, Urbacodon, and Xixiasaurus may form a new clade, suggesting an endemic radiation of troodontids across Asia, including multiple taxa without dental serrations. The discovery of Xixiasaurus in the Xixia Basin may imply that the Xixiasaurus−bearing Majiacun Formation is Campanian in age.
Źródło:
Acta Palaeontologica Polonica; 2010, 55, 3; 381-388
0567-7920
Pojawia się w:
Acta Palaeontologica Polonica
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
A reassessment of Kelmayisaurus petrolicus, a large theropod dinosaur from the Early Cretaceous of China
Autorzy:
Brusatte, S.L.
Benson, R.B.J.
Xu, X.
Powiązania:
https://bibliotekanauki.pl/articles/21593.pdf
Data publikacji:
2012
Wydawca:
Polska Akademia Nauk. Instytut Paleobiologii PAN
Tematy:
paleontology
Kelmayisaurus petrolicus
large theropod dinosaur
theropod dinosaur
Early Cretaceous
China
Dinosauria
Theropoda
Carcharodontosauridae
anatomy
Cretaceous
Opis:
The Early Cretaceous fossil record of large−bodied theropods from Asia is poor, hindering comparison of Asian predatory dinosaur faunas with those from other continents. One of the few large Asian theropod specimens from this interval is a partial skull (maxilla and dentary) from the Lianmugin Formation (?Valanginian–Albian), the holotype of Kelmayisaurus petrolicus. Most authors have either considered this specimen as an indeterminate basal tetanuran or a nomen dubium. We redescribe K. petrolicus and note that it possesses a single autapomorphy (a deep accessory groove on the lateral surface of the anterior dentary), as well as a unique combination of characters that differentiates it from other theropods, affirming its validity. A phylogenetic analysis recovers K. petrolicus as a basal carcharodontosaurid, which is supported by various features: very deep interdental plates (a carcharodontosaurid synapomorphy), fused interdental plates (present in carchardontosaurids and a limited number of other theropods), and the absence of diagnostic features of other clades of large−bodied theropods such as abelisaurids, megalosauroids, and coelurosaurs. As such,Kelmayisaurusis the second known carcharodontosaurid from Asia, and further evidence that this clade represented a global radiation of large−bodied predators during the Early–mid Cretaceous.
Źródło:
Acta Palaeontologica Polonica; 2012, 57, 1
0567-7920
Pojawia się w:
Acta Palaeontologica Polonica
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
An efficient connected dominating set algorithm in WSNs based on the induced tree of the crossed cube
Autorzy:
Zhang, J.
Xu, L.
Zhou, S. M.
Wu, W.
Ye, X.
Powiązania:
https://bibliotekanauki.pl/articles/330830.pdf
Data publikacji:
2015
Wydawca:
Uniwersytet Zielonogórski. Oficyna Wydawnicza
Tematy:
wireless sensor networks
connected dominating set
induced tree
approximation algorithm
crossed cube
bezprzewodowa sieć sensorowa
podgrafy indukowane
algorytm aproksymacyjny
Opis:
The connected dominating set (CDS) has become a well-known approach for constructing a virtual backbone in wireless sensor networks. Then traffic can forwarded by the virtual backbone and other nodes turn off their radios to save energy. Furthermore, a smaller CDS incurs fewer interference problems. However, constructing a minimum CDS is an NP-hard problem, and thus most researchers concentrate on how to derive approximate algorithms. In this paper, a novel algorithm based on the induced tree of the crossed cube (ITCC) is presented. The ITCC is to find a maximal independent set (MIS), which is based on building an induced tree of the crossed cube network, and then to connect the MIS nodes to form a CDS. The priority of an induced tree is determined according to a new parameter, the degree of the node in the square of a graph. This paper presents the proof that the ITCC generates a CDS with a lower approximation ratio. Furthermore, it is proved that the cardinality of the induced trees is a Fibonacci sequence, and an upper bound to the number of the dominating set is established. The simulations show that the algorithm provides the smallest CDS size compared with some other traditional algorithms.
Źródło:
International Journal of Applied Mathematics and Computer Science; 2015, 25, 2; 295-309
1641-876X
2083-8492
Pojawia się w:
International Journal of Applied Mathematics and Computer Science
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
An investigation of mechanical properties of recycled coarse aggregate concrete
Badania właściwości mechanicznych betonu kruszywowego wtórnego wykonanego z użyciem kruszywa gruboziarnistego także pochodzenia wtórnego
Autorzy:
Deng, X. H.
Lu, Z. L.
Li, P.
Xu, T.
Powiązania:
https://bibliotekanauki.pl/articles/962346.pdf
Data publikacji:
2016
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
beton
kruszywo gruboziarniste
kruszywo z recyklingu
stosunek wodno-cementowy
wskaźnik zamiany
wytrzymałość na ściskanie
wytrzymałość na rozciąganie
rozłupywanie
concrete
coarse aggregate
recycled aggregate
water-cement ratio
replacement rate
compression strength
tensile strength
splitting
Opis:
Recycling construction and demolition waste not only reduces project costs; and saves natural resources, but also solves the environmental threat caused by construction waste disposal. In this paper, C25 waste road concrete is used as an experimental material, the uniaxial compression strength and tensile splitting strength of C25 RAC whose coarse aggregate replacement rate is 0%, 25%, 50%, 75%, and 100% are tested under the condition that the water-to-cement ratio is 0.47, 0.55 and 0.61. The results show: (1) the uniaxial compression strength and tensile splitting strength decrease with the increase of RAC; (2) for concrete with the same water-to-cement ratio, when the coarse aggregate replacement rate changes from 0% to 50%, the uniaxial compression strength and tensile splitting strength of RAC changes slightly. When the coarse aggregate replacement rate changes from 50% to 100%, the uniaxial compression strength and tensile splitting strength of RAC decreases rapidly.
Recykling odpadów budowlanych oraz materiałów pochodzących z rozbiórek nie tylko zmniejsza koszty projektu i oszczędza zasoby naturalne, ale również zażegnuje zagrożenie dla środowiska spowodowane przez składowanie odpadów budowlanych. Szczególnie w odniesieniu do właściwości mechanicznych betonu z kruszywa gruboziarnistego poddanego recyklingowi, odpady betonu drogowego C25 są używane jako materiał eksperymentalny, pod warunkiem że stosunek wody do cementu wynosi 0,47, 0,55 i 0,61 oraz są przygotowane próbki betonu o wskaźniku zamiany betonu z kruszywa gruboziarnistego poddawanego recyklingowi wynoszącym 25%, 50%, 75% i 100% betonu z naturalnego kruszywa gruboziarnistego. Próbka to sześcian o wymiarach 150 × 150 × 150 mm. Przygotowaliśmy 6 próbek dla każdego wskaźnika zamiany betonu z kruszywa gruboziarnistego poddawanego recyklingowi. Przygotowano łącznie 90 próbek betonu. Próbki zostały usunięte 24 godziny po ustawieniu oraz były utwardzane w temperaturze 20°C i wilgotności 90% przez 28 dni. Następnie przeprowadzono próby ściskania jednoosiowego oraz wytrzymałości na rozciąganie przez rozłupywanie betonu z kruszywa poddawanego recyklingowi C25.
Źródło:
Archives of Civil Engineering; 2016, 62, 4/II; 19-34
1230-2945
Pojawia się w:
Archives of Civil Engineering
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Bacterial communities in PM2.5 and PM10 in broiler houses at different broiler growth stages in spring
Autorzy:
Zhang, J.
Li, Y.
Xu, E.
Jiang, L.
Tang, J.
Li, M.
Zhao, X.
Chen, G.
Zhu, H.
Yu, X.
Zhang, X.
Powiązania:
https://bibliotekanauki.pl/articles/2087465.pdf
Data publikacji:
2019
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
bacterial communities
broilers
high-throughput sequencing
particulate matter
Źródło:
Polish Journal of Veterinary Sciences; 2019, 3; 495-504
1505-1773
Pojawia się w:
Polish Journal of Veterinary Sciences
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Characterization of Phyllosticta hostae causing Phyllosticta leaf spot on spider lily in China
Autorzy:
Yi, R.H.
Gan, L.J.
Chen, J.
Xu, X.L.
Powiązania:
https://bibliotekanauki.pl/articles/66837.pdf
Data publikacji:
2015
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
Phyllosticta hostae
leaf spot
plant disease
spider lily
Hymenocallis littoralis
tropical plant
herb
China
Opis:
Leaf spot disease on the spider lily [Hymenocallis littoralis (Jacq.) Salisb.] continues to cause serious problems in China. To confirm the pathogen, the pathogenicity of isolates from diseased leaves was tested according to Koch’s postulates. The isolates were tentatively identified using morphological characteristics and confirmation was done by phylogenetic analysis of the translation elongation factor 1-alpha gene (TEF1), the actin gene (ACT), and internal transcibed spacer (ITS) sequences using neighbor-joining (NJ), maximum parsimony (MP), and Bayesian inference (BI) methods. The pathogen was identified as Phyllosticta hostae. Molecular analysis indicated very little diversity in the TEF1, ACT, and ITS gene. This is the first report of P. hostae causing leaf spot disease on spider lily in China.
Źródło:
Journal of Plant Protection Research; 2015, 55, 4
1427-4345
Pojawia się w:
Journal of Plant Protection Research
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Comparative study of fault-tolerant performance of a segmented rotor SRM and a conventional SRM
Autorzy:
Sun, X.
Xue, Z.
Han, S.
Chen, L.
Xu, X.
Yang, Z.
Powiązania:
https://bibliotekanauki.pl/articles/200893.pdf
Data publikacji:
2017
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
fault tolerance
segmented rotor SRM
phase failure
finite element method
dynamic performance
rotor segmentowy SRM
metoda elementów skończonych
wydajność dynamiczna
tolerancja błędu
awaria jednej fazy
Opis:
Due to the separation of magnetic field, electrical isolation and thermal isolation, motor drives possess a high fault-tolerance characteristic. In this paper, comparative study of mutual inductance between the proposed segmented rotor switched reluctance motor (SSRM) and the conventional switched reluctance motor (SRM) is carried out first, illustrating that the proposed SSRM has less mutual inductance between phases than the conventional SRM. In addition, if winding faults or power converter faults lead to phase failure, a comparative analysis on fault-tolerant performance under phase failure condition between the proposed SSRM and the conventional SRM is simulated in detail using the finite element method (FEM). Simulation results reveal that dynamic performance of the proposed SSRM, including output torque and phase current, is better than that of the conventional SRM. That is, the capacity of operating with the fault under phase failure condition in the proposed SSRM is superior to that in the conventional SRM.
Źródło:
Bulletin of the Polish Academy of Sciences. Technical Sciences; 2017, 65, 3; 375-381
0239-7528
Pojawia się w:
Bulletin of the Polish Academy of Sciences. Technical Sciences
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Effect of gas holdup on the efficiency of cyclonic-static microbubble flotation column for oily wastewater treatment
Autorzy:
Ning, K.
Huang, G.
Xu, H.
Wu, L.
Li, X.
Wang, Y.
Powiązania:
https://bibliotekanauki.pl/articles/208139.pdf
Data publikacji:
2018
Wydawca:
Politechnika Wrocławska. Oficyna Wydawnicza Politechniki Wrocławskiej
Tematy:
bubble columns
efficiency
flotation
wastewater treatment
oil water separation
kolumna flotacyjna
efektywność
flotacja
oczyszczanie ścieków
separacja wody
Opis:
A cyclonic-static microbubble flotation column of a novel construction was used in oil-water separation fields and has high efficiency for oil-water separation. The gas holdup is a key parameter for the evaluation of the performance of a flotation column. The gas holdup, closely related to the bubble size, bubble velocity and superficial gas velocity, is one of the most important parameters characterizing the hydrodynamics of a bubble column. The effect of gas holdup in a cyclonic-static microbubble flotation column was investigated. In addition, several operating parameters such as the circulating pressure, superficial gas velocity, and frother consumption were also investigated. The gas holdup was positively correlated to the superficial gas velocity. The gas holdup of clean water and oil wastewater increased along with the increase of the frother consumption. The separation mechanism of cyclonic-static microbubble flotation column was analyzed.
Źródło:
Environment Protection Engineering; 2018, 44, 2; 5-17
0324-8828
Pojawia się w:
Environment Protection Engineering
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Effects of organic cultivation pattern on tomato production: plant growth characteristics, quality, disease resistance, and soil physical and chemical properties
Autorzy:
Feng, X.
Xu, Y.
Liu, D.
Peng, L.
Dong, J.
Yao, S.
Feng, Y.
Feng, Z.
Li, F.
Hu, B.
Powiązania:
https://bibliotekanauki.pl/articles/12703546.pdf
Data publikacji:
2020
Wydawca:
Uniwersytet Przyrodniczy w Lublinie. Wydawnictwo Uniwersytetu Przyrodniczego w Lublinie
Opis:
Tomato (Solanum lycopersicum L.) is one of the world’s most important cultivated vegetable. In the traditional cultivation methods, the excessive use of pesticides and fertilizers leads to an imbalance of nutrient elements in the soil, an increase in pests and diseases and a decrease in vegetable quality and yield. In the face of increasingly serious environmental and food problems, organic agriculture is considered to be an effective solution. In this experiment, the effects of organic cultivation patterns on the growth, quality, disease resistance in tomatoes, and the physical and chemical properties of soil were studied by different treatments. The results showed that the application of effective microorganisms (EM) bio-organic fertilizer in the cultivation process can significantly improve the yield, quality, and antioxidant enzyme activity of tomato. The use of straw mulching was found to significantly increase the growth, chlorophyll content, transpiration rate, and soluble sugar content of tomatoes. The application of EM bio-organic fertilizer or straw mulching significantly increased the activity of antioxidant enzyme and the expression of LeCHI gene in tomato leaves and enhanced tomato resistance to diseases. Organic production practices were found to significantly improve the soil.
Źródło:
Acta Scientiarum Polonorum. Hortorum Cultus; 2020, 19, 1; 71-84
1644-0692
Pojawia się w:
Acta Scientiarum Polonorum. Hortorum Cultus
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Estimation of vehicle sideslip angle via pseudo-multisensor information fusion method
Autorzy:
Chen, T.
Chen, L.
Cai, Y.
Xu, X.
Powiązania:
https://bibliotekanauki.pl/articles/220661.pdf
Data publikacji:
2018
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
vehicle state estimation
sideslip angle
recursive least squares
multi-sensor information fusion
pseudo-measurements
Opis:
This paper presents a novel sideslip angle estimator based on the pseudo-multi-sensor fusion method. The kinematics-based and dynamics-based sideslip angle estimators are designed for sideslip angle estimation. Also, considering the influence of ill-conditioned matrix and model uncertainty, a novel sideslip angle estimator is proposed based on the wheel speed coupling relationship using a modified recursive least squares algorithm. In order to integrate the advantages of above three sideslip angle estimators, drawing lessons from the multisensory information fusion technology, a novel thinking of sideslip angle estimator design is presented through information fusion of pseudo-multi-sensors. Simulations and experiments were carried out, and effectiveness of the proposed estimation method was verified.
Źródło:
Metrology and Measurement Systems; 2018, 25, 3; 499-516
0860-8229
Pojawia się w:
Metrology and Measurement Systems
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Fast near-infrared palmprint recognition using nonnegative matrix factorization extreme learning machine
Autorzy:
Xu, X.
Zhang, X.
Lu, L.
Deng, W.
Zuo, K
Powiązania:
https://bibliotekanauki.pl/articles/173572.pdf
Data publikacji:
2014
Wydawca:
Politechnika Wrocławska. Oficyna Wydawnicza Politechniki Wrocławskiej
Tematy:
extreme learning machine
palmprint recognition
superior speed
support vector machine (SVM)
Opis:
Support vector machine and artificial neural network are widely used in classification applications. Extreme learning machine (ELM) is a novel and efficient learning algorithm based on the generalized single hidden layer feed forward networks, which performs well in classification applications. The research results have shown the superiority of ELM with the existing classical algorithms: support vector machine (SVM) and back propagation neural network. In this study, we firstly propose a novel nonnegative matrix factorization extreme learning machine (NMFELM) to improve the performance of standard ELM method. Then we propose a novel near-infrared palmprint recognition approach based on NMFELM classifier. As the test data, we use the near-infrared palmprint database provided by Hong Kong Polytechnic University. The experimental results demonstrate that the proposed NMFELM method outperforms the standard ELM- and SVM-based methods.
Źródło:
Optica Applicata; 2014, 44, 2; 285-298
0078-5466
1899-7015
Pojawia się w:
Optica Applicata
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Heavy Metal Contamination of Surface Water and Groundwater in and Around Gejiu Tin Mine, Southwest China
Zanieczyszczenie wód powierzchniowych i podziemnych metalami ciężkimi w kopalni Gejiu Tin, południowo-zachodnie Chiny i jej okolicach
Autorzy:
Cheng, X.
Qi, W.
Danek, T.
Matysek, D.
Huang, Q.
Zhao, X.
Zhou, Z.
Fang, R.
Zou, L.
Xu, J.
Powiązania:
https://bibliotekanauki.pl/articles/317873.pdf
Data publikacji:
2016
Wydawca:
Polskie Towarzystwo Przeróbki Kopalin
Tematy:
metale ciężkie
zanieczyszczenie
kopalnia cyny Gejiu
wody powierzchniowe
wody kopalniane
heavy metals
contamination
Gejiu tin mine
surface water
mine water
Opis:
Heavy metal contamination due to mining activity is a global major concern because of its potential health risks to local inhabitants. The heavy metal contamination of surface water and ground water by mining activities in Gejiu tin-polymetallic mining area, Southwest China, was studied. Surface water and ground water were sampled and analyzed using AAS for Cr6+, Cd, As, Hg, Cu, Pb, Zn, Se, Fe and Mn. Analysis of HCO3–, Cl–, SO42–, F– and NO3– in water samples was also undertaken by ion chromatography. It was shown that none of water samples exceeded the guideline of Cr6+, Se and Hg, while the contamination degree of heavy metals was Mn > Fe > Cd > Zn > Pb > As > Cu, all of which were serious contamination except mild contamination for Cu. The ground waters were polluted much worse than surface water.
Zanieczyszczenie metalami ciężkimi spowodowane działalnością kopalń jest światowym problemem z powodu ryzyka utraty zdrowia przez okolicznych mieszkańców. Zbadano zanieczyszczenie wód powierzchniowych oraz wód podziemnych wywołane działalnością kopalni cyny w rejonie górniczym Gejiu, południowo-zachodnie Chiny. Pobrano próbki wody powierzchniowej oraz podziemnej i zanalizowano je ze względu na obecność Cd, As, Hg, Cu, Pb, Zn, Se, Fe oraz Mn. Analizy HCO3–, Cl–, SO42–, F– oraz NO3– w próbkach wody również zostały przeprowadzone za pomocą chromatografii jonowej. Ukazano, że żadna z badanych wód nie przekroczyła dopuszczalnych poziomów dla Cr6+, Se oraz Hg. Jednakże poziom zanieczyszczenia metali ciężkimi tj. Mn > Fe > Cd > Zn > Pb > As > Cu był wysoki, za wyjątkiem niewielkiego przekroczenia norm dla Cu. Wody podziemne były znacznie bardziej zanieczyszczone niż wody powierzchniowe.
Źródło:
Inżynieria Mineralna; 2016, R. 17, nr 1, 1; 93-98
1640-4920
Pojawia się w:
Inżynieria Mineralna
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Identification of medicinal plant Schisandra chinensis using a potential DNA barcode ITS2
Autorzy:
Li, X.-K.
Wang, B.
Han, R.-C.
Zheng, Y.-C.
Yin, H.-B.
Xu, L.
Zhang, J.-K.
Xu, B.-L.
Powiązania:
https://bibliotekanauki.pl/articles/56996.pdf
Data publikacji:
2013
Wydawca:
Polskie Towarzystwo Botaniczne
Tematy:
identification
internal transcribed spacer
medicinal plant
Schisandra chinensis
DNA barcode
Opis:
To test whether the internal transcribed spacer 2 (ITS2) region is an effective marker for using in authenticating of the Schisandra chinensis at the species and population levels, separately. And the results showed that the wild populations had higher percentage of individuals that had substitution of C→A at site 86-bp than the cultivated populations. At sites 10-bp, 37-bp, 42-bp and 235-bp, these bases of the Schisandra sphenanthera samples differed from that of S. chinensis. Two species showed higher levels of inter-specific divergence than intra-specific divergence within ITS2 sequences. However, 24 populations did not demonstrate much difference as inter-specific and intra-specific divergences were concerned. Both S. chinensis and S. sphenanthera showed monophyly at species level, yet the samples of different populations shown polyphyly at population level. ITS2 performed well when using BLAST1 method. ITS2 obtained 100% identification success rates at the species level for S. chinensis, with no ambiguous identification at the genus level for ITS2 alone. The ITS2 region could be used to identify S. chinensis and S. sphenanthera in the “Chinese Pharmacopoeia”. And it could also correctly distinguish 100% of species and 100% of genera from the 193 sequences of S. chinensis. Hence, the ITS2 is a powerful and efficient tool for species identification of S. chinensis.
Źródło:
Acta Societatis Botanicorum Poloniae; 2013, 82, 4
0001-6977
2083-9480
Pojawia się w:
Acta Societatis Botanicorum Poloniae
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Impact of different sources of donor cells upon the nuclear transfer efficiency in Chinese indigenous Meishan pig
Autorzy:
Hua, Z.
Xu, G.
Liu, X.
Bi, Y.
Xiao, H.
Hua, W.
Li, L.
Zhang, L.
Ren, H.
Zheng, X.
Powiązania:
https://bibliotekanauki.pl/articles/31224.pdf
Data publikacji:
2016
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Opis:
Somatic cell nuclear transfer (SCNT) is currently the most efficient and precise method to generate genetically tailored pig models for both agricultural and biomedical research. However, its efficiency is crucially dependent on the source of nuclear donor cells. In this study, we compared the cloning efficiency by using three lines of donor cells that are derived from fetal, newborn and adult fibroblasts of Chinese indigenous Meishan pig. We showed that cleavage rate and blastocyst formation rate of the reconstructed embryos were not significantly different between the fetal (80.7% and 15.6%) and newborn ear skin (77.5% and 12.3%) fibroblast groups (p>0.05), but in both groups these indices were significantly higher than that found in the adult ear skin (70.5% and 8.8%; p<0.05). Reconstructed embryos derived from fetal, newborn, and adult ear skin fibroblasts were transferred to four surrogates, respectively. For the fetal, newborn, and adult ear skin fibroblasts, the number of pregnancies were two (50.0%), two (50.0%), and one (25.0%), respectively, and the number of deliveries were two (50.0%), one (25.0%), and zero (0.0%), respectively. Seven and two cloned piglets were obtained from the fetal and newborn ear skin fibroblasts respectively, while no piglets were obtained from the adult ear skin fibroblasts. Two cloned piglets from the newborn ear skin fibroblasts died shortly after birth because of neonatal asphyxia caused by dystocia. The birth weights of the piglets derived from the fetal and newborn ear skin fibroblasts were 1230.5 and 1310.0g, respectively, which were statistically insignificant (p>0.05), but both were significantly higher than that of the control groups (p<0.05). Microsatellite analyses demonstrated that the genotypes of all cloned piglets were identical to their donor cells. Therefore, cloned pigs were successfully produced using two sources of donor cells isolated from the fetal and newborn ear skin fibroblasts of Meishan piglet, and indicating a better cloning efficiency than that obtained from adult fibroblasts. We concluded that the nuclear donor cell lines have significant impact on the developmental competence of cloned embryos as well as on the cloning efficiency of Meishan pig.
Źródło:
Polish Journal of Veterinary Sciences; 2016, 19, 1
1505-1773
Pojawia się w:
Polish Journal of Veterinary Sciences
Dostawca treści:
Biblioteka Nauki
Artykuł
Wyświetlanie 1-15 z 29

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