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    Wyświetlanie 1-12 z 12
    Tytuł:
    A novel Δ12-fatty acid desaturase gene from methylotrophic yeast Pichia pastoris GS115
    Autorzy:
    Wei, D
    Li, M
    Zhang, X
    Zhou, H
    Xing, L
    Powiązania:
    https://bibliotekanauki.pl/articles/1041169.pdf
    Data publikacji:
    2006
    Wydawca:
    Polskie Towarzystwo Biochemiczne
    Tematy:
    fatty acid desaturase gene
    Pichia pastoris
    Saccharomyces cerevisiae
    linoleic acid
    Opis:
    The methylotrophic yeast Pichia pastoris GS115, a widely used strain in production of various heterologous proteins, especially membrane-bound enzymes, can also produce linoleic and linolenic acids, which indicates the existence of membrane-bound Δ12 and Δ15-fatty acid desaturases. This paper describes the cloning and functional characterization of a novel Δ12-fatty acid desaturase gene from this methylotrophic yeast. The open reading frame of the gene (named Pp-FAD12) is 1263 bp in size and encodes a 420-amino-acid peptide. The deduced Pp-FAD12 protein shows high identity (50-67%) with Δ12-fatty acid desaturases from other fungi. It also shows a high identity (57%) with Δ15-fatty acid desaturase (named Sk-FAD15) from Saccharomyces kluyveri. Expression of Pp-FAD12 in polyunsaturated fatty acids non-producing yeast Saccharomyces cerevisiae demonstrated that its product converted oleic acid (18 : 1) to linoleic acid (18 : 2). This result suggests that Pp-FAD12 encodes a novel Δ12-fatty acid desaturase in P. pastoris GS115. This is the first report about the cloning and functional characterization of Δ12-fatty acid desaturase gene in methylotrophic yeast.
    Źródło:
    Acta Biochimica Polonica; 2006, 53, 4; 753-759
    0001-527X
    Pojawia się w:
    Acta Biochimica Polonica
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Cranial anatomy of the iguanodontoid ornithopod Jinzhousaurus yangi from the Lower Cretaceous Yixian Formation of China
    Autorzy:
    Barrett, P M
    Butler, R.J.
    Xiao-Lin, W.
    Xing, X.
    Powiązania:
    https://bibliotekanauki.pl/articles/19937.pdf
    Data publikacji:
    2009
    Wydawca:
    Polska Akademia Nauk. Instytut Paleobiologii PAN
    Tematy:
    Cretaceous
    Yixian Formation
    Dinosauria
    Jinzhousaurus yangi
    paleontology
    China
    cranial anatomy
    Ornithopoda
    ornithopod
    Liaoning Province
    iguanodontoid ornithopod
    Lower Cretaceous
    dinosaur
    Iguanodontia
    non-avian dinosaur
    Opis:
    The Yixian Formation (Lower Cretaceous) of Liaoning Province, China, is justifiably famous for its exceptionally preserved fauna, which includes a remarkable diversity of non−avian dinosaurs. Here, we provide the first detailed description of the cranial skeleton of the iguanodontian ornithopod Jinzhousaurus yangi. Many previously unrecorded features have been recognised, permitting a new and more robust diagnosis for this taxon, which is based on a suite of autapomorphic features. Jinzhousaurus and an unnamed sauropod represent the largest, but some of the least abundant, animals in the Jehol Biota, a situation that contrasts with many other Lower Cretaceous faunas in which large dinosaurs are common faunal components. This rarity may be due to either palaeoenvironmental constraints or taphonomic bias, although it is not possible to choose between these alternatives on the basis of current data.
    Źródło:
    Acta Palaeontologica Polonica; 2009, 54, 1; 35-48
    0567-7920
    Pojawia się w:
    Acta Palaeontologica Polonica
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Effect of intergrown particle liberation on difficult-to-separate coking coal flotation
    Autorzy:
    Gui, X.
    Liu, J.
    Cao, Y.
    Xing, Y.
    Deng, Y.
    Li, S.
    Powiązania:
    https://bibliotekanauki.pl/articles/109875.pdf
    Data publikacji:
    2016
    Wydawca:
    Politechnika Wrocławska. Oficyna Wydawnicza Politechniki Wrocławskiej
    Tematy:
    liberation
    particle size
    coking coal
    hydrophobicity
    Opis:
    Flotation tests with intergrown particle liberation were conducted to explore a separation method of difficult-to-separate coking coal from the Tangshan Kailuan mine in China. The particle size distribution, density and coal petrography were investigated. The difficult-to-separate coking coal sample resulted in intergrown particles, such as non-liberated coal and rocks. Thus, intergrown coal particle liberation and re-separation tests were conducted. The results showed that grinding time had a great effect on the flotation performance. Grinding prompted coal to dissociate and improve the surface hydrophobic properties of minerals. However, heterogeneous fine silt covered the surface of coal particles when coal was ground too long. The inorganic mineral particles were over-ground and reduced the contact angle of coal. The results of coal rock dissociation and laboratory re-separation tests showed that clean coal combustible recovery increased through intergrown particle liberation and re-separation.
    Źródło:
    Physicochemical Problems of Mineral Processing; 2016, 52, 1; 279-294
    1643-1049
    2084-4735
    Pojawia się w:
    Physicochemical Problems of Mineral Processing
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Flotation behavior of hard-to-separate and high-ash fine coal
    Autorzy:
    Xing, Y.
    Gui, X.
    Liu, J.
    Cao, Y.
    Zhang, Y.
    Li, S.
    Powiązania:
    https://bibliotekanauki.pl/articles/110585.pdf
    Data publikacji:
    2016
    Wydawca:
    Politechnika Wrocławska. Oficyna Wydawnicza Politechniki Wrocławskiej
    Tematy:
    flotation behavior
    fine coal
    Fuerstenau upgrading curve
    energy input
    selectivity
    Opis:
    The flotation behavior of hard-to-separate and high-ash fine coal was investigated using conventional flotation with constant power input. A new flotation process, based on energy input and distribution, was designed to lower the ash content of concentrate. The results obtained using Fourier transform infrared (FTIR) analysis show that the coal samples have good floatability because of many hydrophobic and few hydrophilic functional groups. Under a constant power input, a large number of ash-forming materials floated into a froth product at the start of flotation. Based on the Fuerstenau upgrading curves, it was determined that the 0.25-0.074 mm size fraction range showed the worst selectivity when compared with 0.50-0.25 mm and -0.074 mm size fractions. The desired concentrate with an ash content of 13.98%, 27.59% of ash recovery, and 80.01% combustible matter recovery could be obtained by transferring the excess energy of the flotation-conditioning stage to the pre-conditioning stage and increasing the power input step-by-step in the flotation-conditioning stage at equal total energy consumption.
    Źródło:
    Physicochemical Problems of Mineral Processing; 2016, 52, 2; 703-717
    1643-1049
    2084-4735
    Pojawia się w:
    Physicochemical Problems of Mineral Processing
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Genetic diversity and relationship of Hunan province of China local tree peonies based on SSR markers
    Autorzy:
    Zhang, M.-H.
    Jin, X.-L.
    Wen, Y.-F.
    Shen, S.
    Wen-Xing
    Wu, S.
    Lu, J.-H.
    Ye-Ye
    Powiązania:
    https://bibliotekanauki.pl/articles/12665369.pdf
    Data publikacji:
    2019
    Wydawca:
    Uniwersytet Przyrodniczy w Lublinie. Wydawnictwo Uniwersytetu Przyrodniczego w Lublinie
    Tematy:
    China
    Hunan Province
    plant cultivation
    tree peony
    Paeonia suffruticosa
    ornamental plant
    genetic diversity
    genetic relationship
    SSR marker
    Opis:
    Paeonia sect. Moutan is a wide known ornamental plant in the world. The objective of this study was to provide the theoretical basis for scientific preservation and utilization of tree peony resources of Hunan province of China. Simple sequence repeat (SSR) markers were applied to reveal the genetic diversity and relationship of 21 tree peony resources and 45 domestic and foreign tree peony cultivars. Clear bands, the size of which ranged from 115 bp to 379 bp, were detected with 14 primers. In total, 90 alleles were detected and the number of alleles detected with one primer varied between 5 and 13; the number of effective alleles ranged from 1.183 to 2.070; the polymorphism ratio of each locus was 100%. The observed heterozygosity, which ranged from 0.120 to 0.851 with an average of 0.532, was larger than the expected one, which ranged from 0.090 to 0.470 with an average of 0.300. Shannon index ranged from 0.137 to 0.695 and fixation index ranged from −0.332 to −0.869. The results show abundant genetic diversity in tree peony of Hunan province and SSR markers distinguishing homonymous tree peony resources successfully.
    Źródło:
    Acta Scientiarum Polonorum. Hortorum Cultus; 2019, 18, 4; 213-223
    1644-0692
    Pojawia się w:
    Acta Scientiarum Polonorum. Hortorum Cultus
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Large deformation and stability analysis of a cylindrical rubber tube under internal pressure
    Autorzy:
    Sang, J.
    Xing, S.
    Liu, H.
    Li, X.
    Wang, J.
    Lv, Y.
    Powiązania:
    https://bibliotekanauki.pl/articles/279927.pdf
    Data publikacji:
    2017
    Wydawca:
    Polskie Towarzystwo Mechaniki Teoretycznej i Stosowanej
    Tematy:
    large deformation analysis
    stability analysis
    rubber tube
    nonlinear elastic theory
    Opis:
    Rubber tubes under pressure can undergo large deformations and exhibit a particular nonlinear elastic behavior. In order to reveal mechanical properties of rubber tubes subjected to internal pressure, large deformation analysis and stability analysis have been proposed in this paper by utilizing a modified Gent’s strain energy function. Based on the nonlinear elastic theory, by establishing the theoretical model of a rubber tube under internal pressure, the relationship between the internal pressure and circumferential principal stretch has been deduced. Meanwhile stability analysis of the rubber tube has also been proposed and the relationship between the internal pressure and the internal volume ratio has been achieved. The effects on the deformation by different parameters and the failure reasons of the rubber tube have been discussed, which provided a reasonable reference for the design of rubber tubes.
    Źródło:
    Journal of Theoretical and Applied Mechanics; 2017, 55, 1; 177-188
    1429-2955
    Pojawia się w:
    Journal of Theoretical and Applied Mechanics
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Mechanical behavior of clinched sheet material joints and strength design procedure
    Autorzy:
    He, X.
    Gao, A.
    Yang, H.
    Xing, B.
    Powiązania:
    https://bibliotekanauki.pl/articles/1155166.pdf
    Data publikacji:
    2016-04
    Wydawca:
    Polska Akademia Nauk. Instytut Fizyki PAN
    Tematy:
    46.70.-p
    Opis:
    Clinching is an effective joining technique for lightweight sheet materials that are difficult or impossible to weld. A theoretical model for clinched joints in metal sheets was established and a design method for improving joint strength by selecting different clinching tools was proposed. The analytic model is defined as a function of the neck-thickness and the undercut, which are the key parameters of joint geometry. Based on the analytic model, the design method of clinched joint strength that can satisfy required strength was proposed. Clinching experiments were conducted with 2.00 mm thick aluminium alloy 5052 sheets. Various conditions were used during the clinching process to validate the joint strength model. Tensile-shear strength of clinched joints was measured by a servo-hydraulic testing machine. The calculated joint strength was in good agreement with the experimental results.
    Źródło:
    Acta Physica Polonica A; 2016, 129, 4; 698-700
    0587-4246
    1898-794X
    Pojawia się w:
    Acta Physica Polonica A
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Mechanical Properties and Fracture Analysis of Clinched Joints in Titanium Sheet Materials
    Autorzy:
    He, X.
    Lei, L.
    Zhang, Y.
    Xing, B.
    Powiązania:
    https://bibliotekanauki.pl/articles/1030360.pdf
    Data publikacji:
    2017-01
    Wydawca:
    Polska Akademia Nauk. Instytut Fizyki PAN
    Tematy:
    46.70.-p
    Opis:
    Mechanical fastening techniques are used extensively in different industry fields for joining various materials in the assembly of components and structures. The use of mechanical clinching is of interest to different industries such as aerospace, automotive, packaging and domestic appliance. This, together with increasing use of light-weight materials, has produced a significant increase in the use of mechanical clinching in light-weight structures in recent years. This paper deals with the mechanical properties and fracture analysis of extensible die clinched joints in the dissimilar metal sheets combinations of titanium sheet material and aluminum sheet material. Tensile-shear tests were carried out to characterize the mechanical properties of different clinched joints made of the dissimilar metal sheets combinations. The normal hypothesis tests were performed to examine the rationality of the test data. The load-bearing capacity and failure modes of different titanium sheets clinched joints were studied. Results showed that most titanium sheets clinched joints failed in the neck fracture mode. Results also showed that the load-bearing capacity of clinch joints with titanium as upper sheets is higher than that of the clinched joints with titanium as lower sheets.
    Źródło:
    Acta Physica Polonica A; 2017, 131, 1; 16-19
    0587-4246
    1898-794X
    Pojawia się w:
    Acta Physica Polonica A
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Network resource reallocation strategy based on an improved capacity-load model
    Strategia realokacji zasobów sieciowych oparta o udoskonalony model przepustowości-obciążenia
    Autorzy:
    Zhou, J.
    Huang, N.
    Sun, X.
    Xing, L.
    Zhang, S.
    Powiązania:
    https://bibliotekanauki.pl/articles/300643.pdf
    Data publikacji:
    2015
    Wydawca:
    Polska Akademia Nauk. Polskie Naukowo-Techniczne Towarzystwo Eksploatacyjne PAN
    Tematy:
    network reliability
    maintenance
    resource reallocation
    cascading failures
    capacity-load relationship
    niezawodność sieci
    utrzymanie ruchu
    realokacja zasobów
    awarie kaskadowe
    zależność przepustowość
    obciążenie
    Opis:
    Network resource reallocation is a common way to help restore performance of network systems subject to cascading failures. Majority of current network resource allocation strategies either give little regard to or make impractical assumptions about the relationship between capacity and load of network nodes, despite this relationship is closely related to the propagation of network failures. In this work we present and verify an improved nonlinear network capacity-load model based on the actual relation between network capacity and load. According to the verified model and realistic dynamic characteristics of network loads, we propose a new network resource reallocation strategy for networks under attacks from the perspective of maintenance. The strategy aims to effectively reallocate new capacity to network nodes after cascading failures occur. Both theoretical analysis and empirical studies are performed on three typical types of complex networks. Results show that the proposed network resource reallocation strategy is more efficient in mitigating devastating impact of cascading failures on network performance, in comparison to other three existing network resource reallocation strategies.
    Realokacja zasobów sieci jest powszechnym sposobem, stosowanym w celu przywrócenia działania systemów sieciowych objętych awariami kaskadowymi. Większość współczesnych strategii alokacji zasobów sieciowych kładzie mały nacisk lub czyni niepraktyczne założenia dotyczące zależności między przepustowością i obciążeniem węzłów sieci, choć zależność ta jest ściśle związana z rozchodzeniem się awarii sieci. W niniejszej pracy przedstawiono i zweryfikowano udoskonalony nieliniowy model przepustowości-obciążenia sieci na podstawie rzeczywistej relacji między przepustowością sieci i jej obciążeniem. Na podstawie zweryfikowanych modelu i realistycznych cech dynamicznych obciążeń sieciowych, proponujemy nową strategię realokacji zasobów dla sieci poddawanych atakom z perspektywy utrzymania ruchu. Celem strategii jest skuteczna realokacja nowej przepustowości węzłom sieci po wystąpieniu kaskadowych awarii. Przeprowadzono zarówno teoretyczne analizy, jak i badania empiryczne na trzech typowych rodzajach sieci złożonych. Wyniki pokazują, że proponowana strategia realokacji zasobów sieci jest bardziej skuteczna w zwalczaniu niszczącego wpływu kaskadowych awarii na przepustowość sieci w porównaniu do pozostałych trzech wykorzystywanych strategii realokacji zasobów sieciowych.
    Źródło:
    Eksploatacja i Niezawodność; 2015, 17, 4; 487-495
    1507-2711
    Pojawia się w:
    Eksploatacja i Niezawodność
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    New insights into reliability problems for supply chains management based on conventional reliability model
    Nowe spojrzenie na problemy związane z niezawodnością w zarządzaniu łańcuchami dostaw z punktu widzenia tradycyjnego modelu niezawodności
    Autorzy:
    Jia, X.
    Cui, L.
    Xing, L.
    Powiązania:
    https://bibliotekanauki.pl/articles/301238.pdf
    Data publikacji:
    2018
    Wydawca:
    Polska Akademia Nauk. Polskie Naukowo-Techniczne Towarzystwo Eksploatacyjne PAN
    Tematy:
    supply chain management
    conventional reliability
    supply chain reliability
    reliability system structures
    reliability indexes
    zarządzanie łańcuchami dostaw
    niezawodność w ujęciu tradycyjnym
    niezawodność łańcucha dostaw
    struktury systemu niezawodności
    wskaźniki niezawodności
    Opis:
    The paper aims to find the relationship between conventional reliability and supply chain reliability, and introduce and adapt conventional reliability models to the field of supply chains, expanding the horizon of solving supply chain reliability problems. Based on a comprehensive literature review, the paper summarizes definitions of reliability in supply chain systems and presents reliability system structures and reliability indexes for supply chains. Relationship and differences between conventional reliability and supply chain reliability are shown. Illustrative examples such as the supply chain reliability problem in China are provided to show how to convert a supply chain reliability problem into a conventional reliability problem and then solve it using reliability techniques in conventional reliability.
    Celem artykułu jest znalezienie związku między niezawodnością w ujęciu tradycyjnym a niezawodnością łańcuchów dostaw, a także wprowadzenie i dostosowanie tradycyjnych modeli niezawodności do badań nad łańcuchami dostaw, co pozwoli na rozszerzenie możliwości rozwiązywania problemów dotyczących niezawodności tych ostatnich. W oparciu o obszerny przegląd literatury, w artykule przedstawiono pokrótce definicje niezawodności w systemach łańcucha dostaw oraz omówiono struktury systemów niezawodnościowych i wskaźniki niezawodności dla łańcuchów dostaw. Pokazano zależności i różnice między niezawodnością w rozumieniu tradycyjnym a niezawodnością łańcucha dostaw. Przedstawiono przykład problemu niezawodności łańcucha dostaw zaczerpnięty z realiów chińskich, ilustrujący jak można przekształcić problem niezawodności łańcucha dostaw w tradycyjny problem niezawodnościowy, a następnie rozwiązać go za pomocą technik niezawodnościowych stosowanych w tradycyjnej analizie niezawodności.
    Źródło:
    Eksploatacja i Niezawodność; 2018, 20, 3; 465-470
    1507-2711
    Pojawia się w:
    Eksploatacja i Niezawodność
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Rapid screening of monoclonal antibodies against porcine circovirus type 2 using colloidal gold-based paper test
    Autorzy:
    Jin, Q.Y.
    Feng, L.L.
    Wang, Y.B.
    Li, P.
    Yang, J.F.
    Teng, M.
    Chai, S.J.
    Xing, G.X.
    Zhang, G.P.
    Powiązania:
    https://bibliotekanauki.pl/articles/16539073.pdf
    Data publikacji:
    2022
    Wydawca:
    Polska Akademia Nauk. Czasopisma i Monografie PAN
    Tematy:
    porcine circovirus type 2 (PCV2)
    screening of MAbs
    paper test
    Opis:
    A proof of concept for using paper test as a suitable method in the production of monoclonal antibodies (MAbs) is reported. The paper test which detects antibodies against porcine circovirus type 2 (PCV2) using colloidal gold-labelled capsid protein as the antigen probe was applied exclusively in the screening of anti-PCV2 MAbs. It allowed the detection of 118 single cell clones within 30 min using naked eyes. MAbs with specific binding to authentic epitopes on the virus were selected using a blocking strategy in which the antibody was pre-incubated with PCV2 viral sample before applying to the test paper. Five hybridomas secreting MAbs against the capsid protein were obtained, with only three of them capable of binding to PCV2. The results were validated and confirmed using enzyme-linked immunosorbent assay and immunofluorescence assay. The paper test is simple, rapid, and independent on professional technicians and proves to be an excellent approach for the screening of MAbs against specific targets.
    Źródło:
    Polish Journal of Veterinary Sciences; 2022, 25, 1; 27-34
    1505-1773
    Pojawia się w:
    Polish Journal of Veterinary Sciences
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Strength and Fracture Analysis of Single-Lap Self-Pierce Riveted Joints
    Autorzy:
    He, X.
    Lu, Y.
    Liu, F.
    Xing, B.
    Zeng, K.
    Powiązania:
    https://bibliotekanauki.pl/articles/1192386.pdf
    Data publikacji:
    2015-04
    Wydawca:
    Polska Akademia Nauk. Instytut Fizyki PAN
    Tematy:
    46.70.-p
    Opis:
    Due to the increased use of lightweight sheet materials, there has been a significant increase in the use of self-pierce riveting. This paper deals with the strength and fracture mechanism of single-lap self-pierce riveted joints. The online window technique was introduced in the single-lap self-pierce riveting processes for evaluating the quality of joints. Signals obtained from sensors were amplified and transferred to the data acquisition system which measures, processes and saves the signals. Monotonic tensile tests were carried out to measure the ultimate tensile strengths for the single-lap self-pierce riveted joints. For investigating the fracture mechanism of the single-lap self-pierce riveted joints, the electrolytic polishing and anode film coating were used for dealing with the cross-section. The differential interference contrast method was used for observing the micro-structure of the cross-section of the joints. The distribution of the hardness in the cross-section of the single-lap self-pierce riveted joints was also studied. The normal hypothesis tests were performed to examine the rationality of the test data.
    Źródło:
    Acta Physica Polonica A; 2015, 127, 4; 1424-1426
    0587-4246
    1898-794X
    Pojawia się w:
    Acta Physica Polonica A
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
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