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Wyświetlanie 1-7 z 7
Tytuł:
Influence of oleic acid in different solvent media on BRL 3A cell growth and viability
Autorzy:
Liu, Runqi
Yang, Wei
Xia, Cheng
Chen, Yuanyuan
Gao, Sansi
Dong, Zhihao
Huang, Baoyin
Li, Ruirui
He, Ping
Xu, Chuang
Powiązania:
https://bibliotekanauki.pl/articles/1038377.pdf
Data publikacji:
2018
Wydawca:
Polskie Towarzystwo Biochemiczne
Tematy:
oleic acid
non-alcoholic fatty liver disease
liver lipid deposition
Opis:
Oleic acid (OA) is widely used in pathology studies of hepatocellular lipid deposition. Identifying the effects of different solvents on OA-induced liver lipid deposition would be beneficial for studies on hepatocytes. We treated BRL 3A cells with OA dissolved in different solvents. After 12 h incubation, cell viability was assessed using MTT assays. Reactive oxygen species (ROS), triglyceride (TG) and total cholesterol (TC) counts, and the expression level of glucose regulated protein (GRP78), sterol regulatory element binding protein (SREBP-1C) and fatty acid synthase (FAS) were analyzed. Water, PBS and DMSO were disadvantageous to the dissolution of OA and did not cause an OA-induced response in hepatocytes. In the alcohol+OA-treated cells, the severe ER stress, oxidative stress and cellular fat deposition were significantly increased. BSA promoted cell growth and the cells treated with 1.2% BSA+OA showed a lower grade TG and endoplasmic reticulum stress compared with KOH+OA and alcohol+OA treatments. KOH had no significant influence on BRL 3A cells viability. When treated with OA dissolved in KOH, BRL 3A cells showed a typical hepatocyte damage. KOH was considered the suitable choice for an OA solvent for BRL 3A cells in hepatic lipidosis research.
Źródło:
Acta Biochimica Polonica; 2018, 65, 3; 443-447
0001-527X
Pojawia się w:
Acta Biochimica Polonica
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Energy feature of a multi-flow column flotation process
Autorzy:
Cheng, G.
Yu, Y.
Ma, L.
Xia, W.
Xu, H.
Powiązania:
https://bibliotekanauki.pl/articles/109768.pdf
Data publikacji:
2017
Wydawca:
Politechnika Wrocławska. Oficyna Wydawnicza Politechniki Wrocławskiej
Tematy:
flotation column
turbulent kinetic energy
turbulent dissipation rate
bubble
Opis:
A cyclonic-static micro-bubble flotation column (FCSMC) has been widely used in mineral separation. FCSMC includes countercurrent, cyclone and jet flow mineralization zones in a single column. In this study, the energy feature of the three different zones was compared. The turbulent flow was evaluated in terms of the turbulent kinetic energy (k) and the turbulent dissipation rate (ε). An appropriate computing model was determined by comparing the flow field value measured by PIV with the results of the Fluent numerical simulation. Jet flow separation exhibited the maximum k and ε values among the three columns, whereas counter-current separation displayed the minimum values. The high circulating volumetric flowrate means great energy input and turbulent intensity. The higher turbulent dissipation rate, the smaller the bubble is. The better performance of the FCSMC was mainly attributed to the multiple mineralization steps. The floatability of mineral particles gradually decreases with an increase in flotation time, the mineralization energy gradually increased to overcome the decrease in mineral floatability. By contrast, the countercurrent was beneficial for recovering the coarse particles, and the jet flow was beneficial for recovering the fine particles.
Źródło:
Physicochemical Problems of Mineral Processing; 2017, 53, 2; 1266-1284
1643-1049
2084-4735
Pojawia się w:
Physicochemical Problems of Mineral Processing
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Transcriptomic analysis of different tissues in Korean arborvitae
Autorzy:
Mu, H.
Gao, Y.
Cheng, F.
Lin, L.
Wang, G.
Xia, F.
Powiązania:
https://bibliotekanauki.pl/articles/2077762.pdf
Data publikacji:
2019
Wydawca:
Polska Akademia Nauk. Instytut Dendrologii PAN
Tematy:
Thuja koraiensis
transcriptome
tissue specific
Opis:
Korean arborvitae (Thuja koraiensis) is an evergreen shrub or small tree native to Korea and the extreme Changbai Mountain of China threatened by habitat loss. Due to the limited genomic sources of Ko- rean arborvitae, it is important to explore transcriptome to understand this economically important plant. We used RNA-seq technology to characterize the transcriptome of root, stem and leaf in Korean arborvitae, respectively. Based on the bioinformatics analysis of assembled transcriptome data, transcriptional path- ways and differentially expressed genes (DEGs) were identified. There were 152.26 million reads generated, and 446,568 unigenes with a mean size of 423.51 nt obtained via de novo assembly. Of these, 204,091 unigenes (45.70%) were further annotated by comparison to public protein databases. A total of 63,495 unigenes (14.22%) were associated into 130 pathways by searching against the KEGG database. DEGs analysis identified 11,890, 5,900 and 10,136 DEGs from the comparison of root vs. stem, root vs. leaf and stem vs. leaf, respectively. Besides, photosynthesis, plant hormone signal transduction and metabolism and biosynthesis of sugar and amino acids were changed in different tissues. We obtained 446,568 unigenes in Korean arborvitae and 11,890, 5,900 and 10,136 DEGs from the compar- ison of root vs. stem, root vs. leaf and stem vs. leaf, respectively. These results will aid in understanding and carrying out future studies on the molecular basis of Korean arborvitae and contribute to future artificial production and applications.
Źródło:
Dendrobiology; 2019, 81; 31-39
1641-1307
Pojawia się w:
Dendrobiology
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Expression and immunological evaluation of elongation factor Tu of Streptococcus suis serotype 2
Autorzy:
Xia, X.J.
Wang, L.
Cheng, L.K.
Shen, Z.Q.
Li, S.G.
Wang, J.L.
Powiązania:
https://bibliotekanauki.pl/articles/31256.pdf
Data publikacji:
2017
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Opis:
Streptococcus suis serotype 2 (SS2) is considered as a major pathogen that causes sepsis and meningitis in piglets and humans, but knowledge of its antigenic proteins remains limited so far. The surface-related proteins of pathogens often play significant roles in bacterium-host interactions and infection. Here, we obtained the elongation factor Tu (EF-Tu) gene of Streptococcus suis and constructed the recombinant expression plasmid successfully. The target recombinant plasmid was then expressed in Escherichia coli and the immuno-protection of the recombinant protein was subsequently evaluated as well. The EF-Tu gene of Streptococcus suis is 1197 bp in length, encodes 398 amino acids. The target recombinant EF-Tu (rEF-Tu) protein can recognize the antiserum of Streptococcus suis and can provoke obvious humoral immune responses in rabbits and conferred protection to rabbits against Streptococcus suis ear-vein challenge, implying that the EF-Tu may be used as an attractive candidate antigen for a component of subunit vaccine.
Źródło:
Polish Journal of Veterinary Sciences; 2017, 20, 2
1505-1773
Pojawia się w:
Polish Journal of Veterinary Sciences
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Physics-of-failure and computer-aided simulation fusion approach with a software system for electronics reliability analysis
Metoda i oprogramowanie do analizy niezawodności urządzeń elektronicznych oparte na połączeniu metodologii fizyki uszkodzeń i symulacji komputerowej
Autorzy:
Sun, Bo
Li, Yu
Wang, Zili
Li, Zhifeng
Xia, Quan
Ren, Yi
Feng, Qiang
Yang, Dezhen
Qian, Cheng
Powiązania:
https://bibliotekanauki.pl/articles/301540.pdf
Data publikacji:
2020
Wydawca:
Polska Akademia Nauk. Polskie Naukowo-Techniczne Towarzystwo Eksploatacyjne PAN
Tematy:
physics of failure
reliability analysis
electronics
prognostics and health management
computeraided simulation
software system
fizyka uszkodzeń
analiza niezawodności
urządzenia elektroniczne
prognostyka i zarządzanie zdrowiem
symulacja komputerowa
system oprogramowania
Opis:
Electronics, such as those used in the communication, aerospace and energy domains, often have high reliability requirements. To reduce the development and testing cost of electronics, reliability analysis needs to be incorporated into the design stage. Compared with traditional approaches, the physics of failure (PoF) methodology can better address cost reduction in the design stage. However, there are many difficulties in practical engineering applications, such as processing large amounts of engineering information simultaneously. Therefore, a flexible approach and a software system for assisting designers in developing a reliability analysis based on the PoF method in electronic product design processing are proposed. This approach integrates the PoF method and computer-aided simulation methods, such as CAD, FEM and CFD.The software system integrates functional modules such as product modeling, load-stress analysis and reliability analysis, which can help designers analyze the reliability of electronic products in actual engineering design. This system includes software and hardware that validate the simulation models. Finally, a case study is proposed in which the software system is used to analyze the filter module reliability of an industrial communication system. The results of the analysis indicate that the system can effectively promote reliability and can ensure the accuracy of analysis with high computing efficiency.
Urządzenia elektroniczne, na przykład te używane w łączności, lotnictwie i energetyce, często muszą spełniać wysokie wymagania dotyczące niezawodności. Aby zmniejszyć koszty rozwoju i testowania tego typu urządzeń, należy opracować metodę analizy niezawodności, którą można wykorzystywać już na etapie projektowania. Metodologia fizyki uszkodzeń (PoF) pozwala, lepiej niż tradycyjne podejścia, rozwiązywać problemy związane z niezawodnością już na etapie powstawania projektu. Jednak jej zastosowanie w praktyce inżynierskiej nastręcza wielu trudności, związanych, między innymi, z koniecznością jednoczesnego przetwarzania dużych ilości informacji inżynieryjnych. W związku z tym, w przedstawionej pracy zaproponowano elastyczne podejście oraz system oprogramowania, które mogą być wykorzystywane przez projektantów do opracowania analizy niezawodności produktu elektronicznego w opaciu o PoF na etapie projektowania. Podejście to stanowi połączenie metody PoF i metod symulacji komputerowej, takich jak CAD, FEM i CFD. System oprogramowania zawiera moduły funkcjonalne, takie jak modelowanie produktu, analiza obciążeń, analiza niezawodności i inne, które mogą wspomagać projektantów w analizie niezawodności projektowanych przez nich produktów elektronicznych. Na system ten, oprócz oprogramowania składa się także sprzęt komputerowy, który służy do walidacji modeli symulacyjnych. W artykule przedstawiono studium przypadku, w którym zaproponowany system oprogramowania wykorzystano do analizy niezawodności modułu filtra wykorzystywanego w systemie łączności przemysłowej. Wyniki analizy pokazują, że opracowane oprogramowanie skutecznie poprawia niezawodność urządzeń jak też zapewnia dokładność analizy przy jednoczesnej wysokiej wydajności obliczeniowej.
Źródło:
Eksploatacja i Niezawodność; 2020, 22, 2; 340-351
1507-2711
Pojawia się w:
Eksploatacja i Niezawodność
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
The relationship between manganism and the workplace environment in China
Autorzy:
Wang, Yongyi
Xue, Jian
Cheng, Shuqun
Ding, Yubin
He, Junlin
Liu, Xueqing
Chen, Xuemei
Wang, Yingxiong
Feng, Xiangyu
Xia, Yinyin
Powiązania:
https://bibliotekanauki.pl/articles/2180079.pdf
Data publikacji:
2012-09-01
Wydawca:
Instytut Medycyny Pracy im. prof. dra Jerzego Nofera w Łodzi
Tematy:
manganese
occupational exposure
welding
mining
occupational intoxication
chronic manganism
Opis:
Manganese is a trace element and a cofactor of many enzymes, so it is essential for physiologic functioning, but it is also a neurotoxin at high doses. Manganism is most often caused by occupational exposure. It is manifested by a myriad of signs and symptoms ranging from the neurasthenia syndrome, such as headache and dizziness, to the Parkinson-like syndrome, depending on the blood manganese levels as well as the duration of exposure. We are reporting a case of manganism using both clinical and occupational hygiene investigation methods. The patient presented the neurasthenia syndrome accompanied by hypertonicity of arm muscles and was diagnosed to have mild chronic manganism. Finally, the patient was discharged from the hospital after the treatment had improved her condition. In China, there are many chronic manga nese cases, partly due to a rapid industrial development with great use of Mn and the low self-protection awareness among the workers and the factories management that cannot catch up with the speed of the economical development. Therefore, factories are responsible for improving the conditions at the workplace.
Źródło:
International Journal of Occupational Medicine and Environmental Health; 2012, 25, 4; 501-505
1232-1087
1896-494X
Pojawia się w:
International Journal of Occupational Medicine and Environmental Health
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Evaluation of differential serum expression of three factors and pulmonary function in patients with silicosis
Autorzy:
Zhu, Ying
Duan, Xia Yu
Cheng, Yun Qi
Yao, Xin Jing
Xu, Hong
Zhang, Kui Sheng
Li, Feng Shi
Yang, Fang
Liu, Liang He
Yuan, Xiang Ju
Powiązania:
https://bibliotekanauki.pl/articles/2086531.pdf
Data publikacji:
2021-08-05
Wydawca:
Instytut Medycyny Pracy im. prof. dra Jerzego Nofera w Łodzi
Tematy:
silicosis
pulmonary function
early diagnosis
ROC
PTPN2
factor B
Opis:
ObjectivesSilicosis is a chronic occupational lung disease. As was previously found by the authors, some proteins increased in the lung tissue of activated rats, and protein tyrosine phosphatase non-receptor type 2 (PTPN2), factor B, and vaccinia-related kinase 1 (VRK1) showed highly differential expressions.Material and MethodsIn this study, serum and bronchoalveolar lavage fluid samples were collected from patients with silicosis and healthy people to verify the expression of PTPN2, factor B, and VRK1. The diagnostic value of differentially expressed proteins for silicosis was judged.ResultsThe expression levels of serum PTPN2, VRK1, and factor B in patients with silicosis were significantly higher than those in the control group (p < 0.01). Higher serum PTPN2 and factor B concentrations significantly and negatively correlated with the ratio of forced expiratory volume in 1 s to forced vital capacity (FEV1/FVC), maximum vital capacity (VCmax), FEV1, and FVC, suggesting that the high expression of PTPN2 and factor B is associated with decreased pulmonary ventilation function and restrictive ventilatory impairment in patients with silicosis. All area under curve (AUC) measurements generated from single detection events were >0.744, with PTPN2 reaching the highest value (0.858). The AUC, sensitivity, and specificity for the combined diagnosis using factor B and PTPN2 were 0.907, 86.91% and 85.07%, respectively, for factor B and PTPN2. The 3 differentially expressed proteins are potential classes of predictive biomarkers for silicosis.ConclusionsRegarding the economy and test practicality, the best diagnostic combination is factor B and PTPN2 for the analysis of AUC, sensitivity and specificity.
Źródło:
International Journal of Occupational Medicine and Environmental Health; 2021, 34, 4; 527-540
1232-1087
1896-494X
Pojawia się w:
International Journal of Occupational Medicine and Environmental Health
Dostawca treści:
Biblioteka Nauki
Artykuł
    Wyświetlanie 1-7 z 7

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