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    Wyświetlanie 1-10 z 10
    Tytuł:
    Modelling of the interactions between magnetic nanoparticles in aqueous solutions
    Autorzy:
    Wolak, Wiktor W.
    Drzewiński, Andrzej
    Marć, Maciej
    Dudek, Mirosław R.
    Powiązania:
    https://bibliotekanauki.pl/articles/2202047.pdf
    Data publikacji:
    2022
    Wydawca:
    Politechnika Częstochowska. Wydawnictwo Politechniki Częstochowskiej
    Tematy:
    iron oxide nanoparticles
    aqueous base solutions
    stability of magnetic suspensions
    nanocząstki tlenku żelaza
    stabilność zawiesin magnetycznych
    Opis:
    The ability of magnetic nanoparticles and their aggregates to form larger structures or new materials is primarily based on the interactions between individual particles. The article analyzes the behavior of spherical nanoparticles Fe3O4 placed in an aqueous base solution as a result of their mutual interactions, i.e. repulsive (electrostatic forces) and attractive (van der Waals forces and dipolar magnetic forces) for the full range of parameter values. Considering the application of magnetic aqueous suspensions in industry or environmental research, the presented method allows for a preliminary selection of the parameters of the dispersed material and the solution so as to obtain a suspension with the desired properties.
    Źródło:
    Journal of Applied Mathematics and Computational Mechanics; 2022, 21, 4; 98--106
    2299-9965
    Pojawia się w:
    Journal of Applied Mathematics and Computational Mechanics
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Characterization of thiamine uptake and utilization in Candida spp. subjected to oxidative stress
    Autorzy:
    Wolak, Natalia
    Tomasi, Massimo
    Kozik, Andrzej
    Rapala-Kozik, Maria
    Powiązania:
    https://bibliotekanauki.pl/articles/1038981.pdf
    Data publikacji:
    2015
    Wydawca:
    Polskie Towarzystwo Biochemiczne
    Tematy:
    vitamin B1
    thiamine diphosphate-dependent enzymes
    antioxidants
    reactive oxygen species
    Candida spp.
    Opis:
    Candida species are associated with an increasing number of life-threatening infections (candidiases), mainly due to the high resistance of these yeast-like fungi to antifungal drugs and oxidative stress. Recently, thiamine (vitamin B1) was found to alleviate stress responses in Saccharomyces cerevisiae; however, thiamine influence on defense systems in pathogenic fungi has never been investigated. The current work was aimed to elucidate the role of thiamine in stress reactions of C. albicans, C. glabrata, C. tropicalis and C. dubliniensis, subjected to hydrogen peroxide treatment. As compared to S. cerevisiae, Candida strains exposed to oxidative stress showed: (i) a much higher dependence on exogenous thiamine; (ii) an increased demand for thiamine diphosphate (TDP) and TDP-dependent enzyme, transketolase; (iii) no changes in gene expression of selected stress markers - superoxide dismutase and catalase - depending on thiamine availability in medium; (iv) a similar decrease of reactive oxygen species (ROS) generation in the presence of thiamine. Moreover, the addition of therapeutic doses of thiamine to yeast culture medium revealed differences in its accumulation between various Candida species. The current findings implicate that the protective action of thiamine observed in S. cerevisiae differs significantly form that in pathogenic Candida strains, both in terms of the cofactor functions of TDP and the effects on fungal defense systems.
    Źródło:
    Acta Biochimica Polonica; 2015, 62, 3; 445-455
    0001-527X
    Pojawia się w:
    Acta Biochimica Polonica
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Kinetic and thermodynamic characterization of the interactions between the components of human plasma kinin-forming system and isolated and purified cell wall proteins of Candida albicans
    Autorzy:
    Seweryn, Karolina
    Karkowska-Kuleta, Justyna
    Wolak, Natalia
    Bochenska, Oliwia
    Kedracka-Krok, Sylwia
    Kozik, Andrzej
    Rapala-Kozik, Maria
    Powiązania:
    https://bibliotekanauki.pl/articles/1038926.pdf
    Data publikacji:
    2015
    Wydawca:
    Polskie Towarzystwo Biochemiczne
    Tematy:
    Candida albicans cell wall
    candidiasis
    contact system
    surface plasmon resonance
    Opis:
    Cell wall proteins of Candida albicans, besides their best known role in the adhesion of this fungal pathogen to host's tissues, also bind some soluble proteins, present in body fluids and involved in maintaining the biochemical homeostasis of the human organism. In particular, three plasma factors - high-molecular-mass kininogen (HK), factor XII (FXII) and prekallikrein (PPK) - have been shown to adhere to candidal cells. These proteins are involved in the surface-contact-catalyzed production of bradykinin-related peptides (kinins) that contribute to inflammatory states associated with microbial infections. We recently identified several proteins, associated with the candidal cell walls, and probably involved in the binding of HK. In our present study, a list of potential FXII- and PPK-binding proteins was proposed, using an affinity selection (on agarose-coupled FXII or PPK) from a whole mixture of β-1,3-glucanase-extrated cell wall-associated proteins and the mass-spectrometry protein identification. Five of these fungal proteins, including agglutinin-like sequence protein 3 (Als3), triosephosphate isomerase 1 (Tpi1), enolase 1 (Eno1), phosphoglycerate mutase 1 (Gpm1) and glucose-6-phosphate isomerase 1 (Gpi1), were purified and characterized in terms of affinities to the human contact factors, using the surface plasmon resonance measurements. Except Gpm1 that bound only PPK, and Als3 that exhibited an affinity to HK and FXII, the other isolated proteins interacted with all three contact factors. The determined dissociation constants for the identified protein complexes were of 10-7 M order, and the association rate constants were in a range of 104-105 M-1s-1. The identified fungal pathogen-host protein interactions are potential targets for novel anticandidal therapeutic approaches.
    Źródło:
    Acta Biochimica Polonica; 2015, 62, 4; 825-835
    0001-527X
    Pojawia się w:
    Acta Biochimica Polonica
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Technical Assessment of River Training Impact on the Raba River Bed Stability on the Selected Longitudinal Section
    Autorzy:
    Łapuszek, Marta
    Wolak, Andrzej
    Powiązania:
    https://bibliotekanauki.pl/articles/125367.pdf
    Data publikacji:
    2019
    Wydawca:
    Polskie Towarzystwo Inżynierii Ekologicznej
    Tematy:
    river training
    river bed stability
    river restoration
    Opis:
    Short assessment and prediction of movement of selected Raba river sections in Southern Poland, using the existing, historical sources is presented in this paper. It was done to the most practicable extent, with regard to the river training structures as groynes and revetments stability structures. The old maps (almost non-existent) and/or aerial photographs of the selected areas and the archival projects of technical Raba river channel regulation were complemented with specific stream power and bed shear stress. In the 8.2 km long study area, four specific sections were studied in the current paper. The general trends over the last fifty years included artificial narrowing of the active channel width with uniform riverbed longitudinal slope. The current studies lead to the conclusion that the narrowed river channel tend to restoring its previous state. However, throughout each analyzed section, the channel-forming processes are varied, mostly related to restoring the local natural longitudinal slope. In Section-1, the process of sediment deposition was observed and it continues till now. In Section-2, the channel-forming processes were carried out with high intensity with tendency to rebuild its previous braided course. Currently, this tendency is continuing. The third Section seems to be the most stable after the river training works. In Section-4 the process of high erosion, especially of the left bank, is observed. The results of the analysis lead to the conclusion that more comprehensive investigation is needed of the whole 8.2-km long reach to assess the river state.
    Źródło:
    Journal of Ecological Engineering; 2019, 20, 9; 145-155
    2299-8993
    Pojawia się w:
    Journal of Ecological Engineering
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Inactivation of α1-proteinase inhibitor by Candida albicans aspartic proteases favors the epithelial and endothelial cell colonization in the presence of neutrophil extracellular traps
    Autorzy:
    Gogol, Mariusz
    Ostrowska, Dominika
    Klaga, Kinga
    Bochenska, Oliwia
    Wolak, Natalia
    Aoki, Wataru
    Ueda, Mitsuyoshi
    Kozik, Andrzej
    Rapala-Kozik, Maria
    Powiązania:
    https://bibliotekanauki.pl/articles/1038860.pdf
    Data publikacji:
    2016
    Wydawca:
    Polskie Towarzystwo Biochemiczne
    Tematy:
    Candida albicans
    aspartic proteases
    α1-proteinase inhibitor
    elastase
    neutrophil extracellular traps
    inflammation
    Opis:
    Candida albicans, a causative agent of opportunistic fungal infections in immunocompromised patients, uses ten secreted aspartic proteases (SAPs) to deregulate the homeostasis of the host organism on many levels. One of these deregulation mechanisms involves a SAP-dependent disturbance of the control over proteolytic enzymes of the host by a system of dedicated proteinase inhibitors, with one important example being the neutrophil elastase and alpha1-proteinase inhibitor (A1PI). In this study, we found that soluble SAPs 1-4 and the cell membrane-anchored SAP9 efficiently cleaved A1PI, with the major cleavage points located at the C-terminal part of A1PI in a close vicinity to the reactive-site loop that plays a critical role in the inhibition mechanism. Elastase is released by neutrophils to the environment during fungal infection through two major processes, a degranulation or formation of neutrophil extracellular traps (NET). Both, free and NET-embedded elastase forms, were found to be controlled by A1PI. A local acidosis, resulting from the neutrophil activity at the infection sites, favors A1PI degradation by SAPs. The deregulation of NET-connected elastase affected a NET-dependent damage of epithelial and endothelial cells, resulting in the increased susceptibility of these host cells to candidal colonization. Moreover, the SAP-catalyzed cleavage of A1PI was found to decrease its binding affinity to a proinflammatory cytokine, interleukin-8. The findings presented here suggest a novel strategy used by C. albicans for the colonization of host tissues and overcoming the host defense.
    Źródło:
    Acta Biochimica Polonica; 2016, 63, 1; 167-175
    0001-527X
    Pojawia się w:
    Acta Biochimica Polonica
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Kolokacje w tekstach prawnych – problemy proceduralne
    Collocations in legal texts – procedural issues
    Autorzy:
    Gębka-Wolak, Małgorzata
    Moroz, Andrzej
    Powiązania:
    https://bibliotekanauki.pl/articles/911200.pdf
    Data publikacji:
    2017-07-01
    Wydawca:
    Uniwersytet im. Adama Mickiewicza w Poznaniu
    Tematy:
    language for special purposes
    legal text
    collocation
    automatic text analysis
    język specjalistyczny
    tekst prawny
    kolokacja
    analiza automatyczna.
    Opis:
    Artykuł wpisuje się w krąg badań, których celem jest przedstawienie ograniczeń w budowaniu konstrukcji składniowych w tekstach prawnych. Za jeden z przejawów tych ograniczeń uznaje się swoistą łączliwość leksykalną, opisywaną za pomocą pojęcia kolokacji. Kolokacją nazywa się wyrażenie składające się z komponentów, których współwystępowanie jest motywowane leksykalnymi ograniczeniami ich łączliwości. Celem poniższego opracowania jest objaśnienie wstępnej, częś- ciowo zautomatyzowanej procedury rozpoznawania kolokacji w podkorpusie tekstów prawnych z dziedziny prawa karnego.
    This article is a part of research dedicated to describe restrictions in the building syntactic structures in legal texts. One of the manifestations of these restrictions is a specific lexical connection, that is described by the concept of collocation. Collocation is defined as an expression consisting of components, whose coexistence is motivated by the lexical restrictions of their connectivity. The aim of this study is to explain the initial partially automated procedure undertaken in recognizing collocations in subcorpus of legal texts – from criminal law.
    Źródło:
    Poznańskie Spotkania Językoznawcze; 2016, 31
    2082-9825
    2450-0259
    Pojawia się w:
    Poznańskie Spotkania Językoznawcze
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Z tygodnia na tydzień, z powieści na powieść, z meczu na mecz jako przykłady nieswobodnych grup syntaktycznych
    From week to week, from novel to novel, from match to match as Examples of Constrained Syntactic Groups
    Autorzy:
    Gębka-Wolak, Małgorzata
    Moroz, Andrzej
    Powiązania:
    https://bibliotekanauki.pl/articles/1045418.pdf
    Data publikacji:
    2014-01-01
    Wydawca:
    Uniwersytet im. Adama Mickiewicza w Poznaniu
    Tematy:
    contemporary Polish language
    lexis
    syntax
    multisegmental lexical unit
    Opis:
    The article has verified a hypothesis suggesting that the sequences mentioned in the title and similar to them are constrained syntactic groups, i.e., they do not form a regular combination of simple signs. The verification has been conducted on the basis of a three-stage procedure taking into account the semantic features and inner qualities of the studied expressions, namely, their composition as well as the structural and linear links between segments. It has been indicated that the components of the studied sequences jointly express meaning which can be considered as a form of perlocution: ‘something changes within fragments of time undergoing observation whose measure is determined by a variable component’. Moreover, it has been demonstrated that the two components of a sequence that can be exchanged can be realized not only by standard measures of time (second, minute, hour, week), but also by nouns referring to events (match, meeting, term). Also, there is a presentation of the basic limitations in the realization of a variable component and a mutual linear arrangement of variable and constant components. A characteristic of the expressions of the type from week to week, from novel to novel, from match to match is supplemented by the remarks concerning their external syntax.
    Źródło:
    Poznańskie Studia Polonistyczne. Seria Językoznawcza; 2014, 21, 2; 9-24
    1233-8672
    2450-4939
    Pojawia się w:
    Poznańskie Studia Polonistyczne. Seria Językoznawcza
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Verification of sensors for yaw rate and lateral acceleration in car ESP system
    Autorzy:
    Gajek, Andrzej
    Janczur, Robert
    Pieniążek, Wiesław
    Wolak, Stanisław
    Powiązania:
    https://bibliotekanauki.pl/articles/263196.pdf
    Data publikacji:
    2020
    Wydawca:
    Sieć Badawcza Łukasiewicz. Przemysłowy Instytut Motoryzacji
    Tematy:
    yaw rate
    lateral acceleration
    operating sensor
    reference sensor
    diagnostic tester
    Opis:
    This study analyzed the possibilities to validate the indications of sensors for yaw rate and lateral acceleration within the scope of extended diagnostic routine of the car ESP system at Vehicle Inspection Stations. The stand tests were performed using the reference sensors and a standard diagnostic tester. The comparison of the yaw rate and lateral acceleration results obtained from the working sensor under examination, recorded with the Vbox unit, and the results from the reference sensor demonstrated a good conformity both in quality and quantity. But these tests demonstrated also a pure conformity (both in quality and quantity) with runs obtained from tester. The Root Mean Square (RMS) was used as factor of qualitative estimation. These inconveniences were caused by in sampling frequency incompatibility and lack of recording synchronization for signals from the tester and from reference sensors. The question of primary importance is a firmware modification for selected testers. Such a tester should be able to record signals from a reference sensor and have a set sampling frequency for signals from a working sensor. On the basis of already carried out research analyses the suggested frequency should be no lower than approximately 10 Hz. It resulted from a spectral analysis carried out for possessed runs maintaining the Nyquist limit frequency.
    Źródło:
    Archiwum Motoryzacji; 2020, 88, 2; 61-76
    1234-754X
    2084-476X
    Pojawia się w:
    Archiwum Motoryzacji
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    The action of ten secreted aspartic proteases of pathogenic yeast Candida albicans on major human salivary antimicrobial peptide, histatin 5
    Autorzy:
    Bochenska, Oliwia
    Rapala-Kozik, Maria
    Wolak, Natalia
    Aoki, Wataru
    Ueda, Mitsuyoshi
    Kozik, Andrzej
    Powiązania:
    https://bibliotekanauki.pl/articles/1038755.pdf
    Data publikacji:
    2016
    Wydawca:
    Polskie Towarzystwo Biochemiczne
    Tematy:
    secreted aspartic proteases
    Sap
    Candida albicans
    antimicrobial peptides
    histatin 5
    candidiasis
    Opis:
    Candida albicans, belonging to the most common fungal pathogens of humans, exploits many virulence factors to infect the host, of which the most important is a family of ten secreted aspartic proteases (Saps) that cleave numerous peptides and proteins, often deregulating the host's biochemical homeostasis. It was recently shown that C. albicans cells can inactivate histatin5 (His5), a salivary histidine-rich anticandidal peptide, through the hydrolytic action of Saps. However, the current data on this subject are incomplete as only four out of ten Saps have been studied with respect to hydrolytic processing of His5 (Sap2, Sap5, Sap9-10). The aim of the study was to investigate the action of all Saps on His5 and to characterize this process in terms of peptide chemistry. It was shown that His5 was degraded by seven out of ten Saps (Sap1-4, Sap7-9) over a broad range of pH. The cleavage rate decreased in an order of Sap2>Sap9>Sap3>Sap7>Sap4>Sap1>Sap8. The degradation profiles for Sap2 and Sap9 were similar to those previously reported; however, in contrast to the previous study, Sap10 was shown to be unable to cleave His5. On a long-time scale, the peptide was completely degraded and lost its antimicrobial potential but after a short period of Sap treatment several shorter peptides (His1-13, His1-17, His1-21) that still decreased fungal survival were released. The results, presented hereby, provide extended characteristics of the action of C. albicans extracellular proteases on His5. Our study contribute to deepening the knowledge on the interactions between fungal pathogens and the human host.
    Źródło:
    Acta Biochimica Polonica; 2016, 63, 3; 403-410
    0001-527X
    Pojawia się w:
    Acta Biochimica Polonica
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
      Wyświetlanie 1-10 z 10

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