Autor: Wojtal, Izabela - Katalog OPAC zbiorów

Skocz do pozycji: 1.

Tytuł:: Analysis of potyvirus terminal protein VPg-transgenic Arabidopsis thaliana plants
Autorzy:: Wojtal, Izabela
Piontek, Paulina
Grzela, Renata
Jarmołowski, Artur
Zagórski, Włodzimierz
Chroboczek, Jadwiga
Powiązania:: https://bibliotekanauki.pl/articles/1039885.pdf
Data publikacji:: 2011
Wydawca:: Polskie Towarzystwo Biochemiczne
Tematy:: VPg protein
Potyvirus
pathogen-derived resistance
transgenic plant
Opis:: Virus-coded VPg protein of Potato virus Y (PVY) does not have homologs apart from other VPgs. Since VPg is indispensable for the potyvirus life cycle, it appeared a good candidate for eliciting pathogen-derived resistance to PVY. Following agroinfection used to obtain PVY VPg-transgenic Arabidopsis thaliana plants, only few transgenic seeds were recovered giving rise to six transgenic plants that contained the VPg gene with the correct sequence. They generated VPg mRNA, but VPg protein was not detected. Some plants were immune to PVY infection suggesting post-transcriptional gene silencing. However, the likely PVY VPg toxicity exerted at an early stage of transformed seeds development precludes its use for engineering pathogen-derived resistance.
Źródło:: Acta Biochimica Polonica; 2011, 58, 3; 349-353
0001-527X
Pojawia się w:: Acta Biochimica Polonica
Dostawca treści:: Biblioteka Nauki

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Skocz do pozycji: 2.

Tytuł:: Certain protein transducing agents convert translocated proteins into cell killers
Autorzy:: Tcherniuk, Siergiej
Fiser, Anne-Laure
Derouazi, Madiha
Toussaint, Bertrand
Wang, Yan
Wojtal, Izabela
Kondo, Eisaku
Szolajska, Ewa
Chroboczek, Jadwiga
Powiązania:: https://bibliotekanauki.pl/articles/1039725.pdf
Data publikacji:: 2012
Wydawca:: Polskie Towarzystwo Biochemiczne
Tematy:: cytotoxic protein
cell transduction
protein delivery
cell translocation
transducing peptides
Opis:: The majority of proteins are unable to translocate into the cell interior. Hence for peptide- and protein-based therapeutics a direct intracytoplasmic delivery with the aid of transducing agents is an attractive approach. We wanted to deliver to the cell interior a putatively cytotoxic protein VPg. Protein transduction was achieved in vitro with three different commercial products. However, in our hands, delivery of various control proteins without known deleterious effects, as well as of protein VPg, always induced cell death. Finally, we used a novel transducing peptide Wr-T, which was not toxic to cultured cells, even in a quite large range of concentrations. Most importantly, control protein delivered to cells in culture did not display any toxicity while VPg protein exerted a strong cytotoxic effect. These data show that results obtained with cell-penetrating agents should be interpreted with caution.
Źródło:: Acta Biochimica Polonica; 2012, 59, 3; 433-439
0001-527X
Pojawia się w:: Acta Biochimica Polonica
Dostawca treści:: Biblioteka Nauki

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