Autor: Wiśniewska, Anita - Katalog OPAC zbiorów

Skocz do pozycji: 1.

Tytuł:: Production of oleanolic acid glycosides by hairy root established cultures of Calendula officinalis L.
Autorzy:: Długosz, Marek
Wiktorowska, Ewa
Wiśniewska, Anita
Pączkowski, Cezary
Powiązania:: https://bibliotekanauki.pl/articles/1039552.pdf
Data publikacji:: 2013
Wydawca:: Polskie Towarzystwo Biochemiczne
Tematy:: Marigold
Agrobacterium rhizogenes
hairy roots
triterpenic saponins
oleanolic acid
transformation
Opis:: In order to initiate hairy root culture initiation cotyledons and hypocotyls of Calendula officinalis L. were infected with Agrobacterium rhizogenes strain ATCC 15834 or the same strain containing pCAMBIA 1381Z vector with β-glucuronidase reporter gene under control of promoter of NIK (Nematode Induced Kinase) gene. The efficiency of induction of hairy roots reached 33.8% for cotyledons and 66.6% for hypocotyls together for both transformation experiments. Finally, eight control and nine modified lines were established as a long-term culture. The hairy root cultures showed the ability to synthesize oleanolic acid mainly (97%) as glycosides; control lines contained it at the average 8.42 mg · g-1 dry weight in tissue and 0.23 mg · dm-3 in medium; modified lines: 4.59 mg · g-1 for the tissue, and 0.48 mg · dm-3 for the medium. Additionally lines showed high positive correlation between dry/fresh weight and oleanolic acid concentration in tissue. Using the Killiani mixture in acidic hydrolysis of oleanolic acid glycosides released free aglycones that were partially acetylated in such conditions.
Źródło:: Acta Biochimica Polonica; 2013, 60, 3; 467-473
0001-527X
Pojawia się w:: Acta Biochimica Polonica
Dostawca treści:: Biblioteka Nauki

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Tytuł:: Metabolic transformations of antitumor imidazoacridinone, C-1311, with microsomal fractions of rat and human liver
Autorzy:: Wiśniewska, Anita
Chrapkowska, Agnieszka
Kot-Wasik, Agata
Konopa, Jerzy
Mazerska, Zofia
Powiązania:: https://bibliotekanauki.pl/articles/1040879.pdf
Data publikacji:: 2007
Wydawca:: Polskie Towarzystwo Biochemiczne
Tematy:: antitumor drug
imidazoacridinone
C-1311
P450 isoenzymes
microsomes
Symadex
in vitro metabolism
Opis:: The imidazoacridinone derivative C-1311 is an antitumor agent in Phase II clinical trials. The molecular mechanism of enzymatic oxidation of this compound in a peroxidase model system was reported earlier. The present studies were performed to elucidate the role of rat and human liver enzymes in metabolic transformations of this drug. C-1311 was incubated with different fractions of liver cells and the reaction mixtures were analyzed by RP-HPLC. We showed that the drug was more sensitive to metabolism with microsomes than with cytosol or S9 fraction of rat liver cells. Incubation of C-1311 with microsomes revealed the presence of four metabolites. Their structures were identified as dealkylation product, M0, as well as a dimer-like molecule, M1. Furthermore, we speculate that the hydroxyl group was most likely substituted in metabolite M3. It is of note that a higher rate of transformation was observed for rat than for human microsomes. However, the differences in metabolite amounts were specific for each metabolite. The reactivity of C-1311 with rat microsomes overexpressing P450 isoenzymes, of CYP3A and CYP4A families was higher than that with CYP1A and CYP2B. Moreover, the M1 metabolite was selectively formed with CYP3A, whereas M3 with CYP4A. In conclusion, this study revealed that C-1311 varied in susceptibility to metabolic transformation in rat and human cells and showed selectivity in the metabolism with P450 isoenzymes. The obtained results could be useful for preparing the schedule of individual directed therapy with C-1311 in future patients.
Źródło:: Acta Biochimica Polonica; 2007, 54, 4; 831-838
0001-527X
Pojawia się w:: Acta Biochimica Polonica
Dostawca treści:: Biblioteka Nauki

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Skocz do pozycji: 3.

Tytuł:: Od „wjeżdżania w bramy” do „otwierania (się) bramy”. Przykład animacji kultury upełnomocniających głos dziecka w procesie rewitalizacji miejskiej
From “entering the gates” to “opening ourselves up the gates”. The example of cultural animations empowering children’s’ voice in the process of urban revitalization
Autorzy:: Gulczyńska, Anita
Wiśniewska-Kin, Monika
Powiązania:: https://bibliotekanauki.pl/articles/1386995.pdf
Data publikacji:: 2020-11-18
Wydawca:: Uniwersytet Gdański. Wydawnictwo Uniwersytetu Gdańskiego
Tematy:: urban revitalization
empowerment
cultural animation
exclusion
inclusion
Opis:: The subject of our study is the positionality of children in the process of urban revitalization. We understand the city as a learning space. From our point of view changes introduced into that space are always interventions into children’s learning processes. In the article we present an empirically based reconstruction of practices of community cultural animations that aim at empowering children’s voice in the processes of revitalization. Konrad Dworakowski’s and Pinokio Theatre’s community cultural animations, realised from 2014 to 2019 in Łódź, offer interesting examples of such practices. Even though we build or argument on the basis of observations collected in Łódź, it can be reliably reflected in discussions on educational issues in urban changes in other post-industrial cities.
Źródło:: Problemy Wczesnej Edukacji; 2020, 50, 3; 55-65
1734-1582
2451-2230
Pojawia się w:: Problemy Wczesnej Edukacji
Dostawca treści:: Biblioteka Nauki

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Tytuł:: Dyskurs edukacyjny w badaniach. Próba systematyzacji perspektyw badawczych
Educational discourse in the research. Draft framework of research perspectives
Autorzy:: Gulczyńska, Anita
Wiśniewska-Kin, Monika
Powiązania:: https://bibliotekanauki.pl/articles/1387964.pdf
Data publikacji:: 2017-02-21
Wydawca:: Uniwersytet Gdański. Wydawnictwo Uniwersytetu Gdańskiego
Tematy:: wczesna edukacja
dyskurs edukacyjny
perspektywa optymalizująca
perspektywa analityczna
perspektywa krytyczna
Opis:: Our aim throughout the contribution was to systemize perspectives employed by the researchers in the studies of educational discourse. Three perspectives of empirical insight into educational discourses were reconstructed in the course of our analysis of selected research examples: optimizing, analytic and critical. The criterion underpinning the analysis was a pragmatic function of the studies. Hence, the links between the choice of research perspective, results of research and practical implications for school improvement were discussed.
Źródło:: Problemy Wczesnej Edukacji; 2017, 36, 1; 7-20
1734-1582
2451-2230
Pojawia się w:: Problemy Wczesnej Edukacji
Dostawca treści:: Biblioteka Nauki