Autor: Wawrzyniak, Mikołaj - Katalog OPAC zbiorów

Skocz do pozycji: 1.

Tytuł:: Możliwości gromadzenia leśnych zasobów genowych w warunkach ex situ
Feasibility of the forest tree species genetic resources ex situ conservation
Autorzy:: Chmielarz, Paweł
Suszka, Jan
Bujarska-Borkowska, Barbara
Lewandowski, Andrzej
Wawrzyniak, Mikołaj
Kotlarski, Szymon
Hazubska-Przybył, Teresa
Litkowiec, Monika
Plitta-Michalak, Beata
Michalak, Marcin
Powiązania:: https://bibliotekanauki.pl/articles/2201308.pdf
Data publikacji:: 2020-12-07
Wydawca:: Instytut Hodowli i Aklimatyzacji Roślin
Źródło:: Biuletyn Instytutu Hodowli i Aklimatyzacji Roślin; 2018, 283; 29-30
0373-7837
2657-8913
Pojawia się w:: Biuletyn Instytutu Hodowli i Aklimatyzacji Roślin
Dostawca treści:: Biblioteka Nauki

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Skocz do pozycji: 2.

Tytuł:: Stimulation of somatic embryo growth and development in Picea spp. by polyethylene glycol
Autorzy:: Hazubska-Przybył, Teresa
Wawrzyniak, Mikołaj
Powiązania:: https://bibliotekanauki.pl/articles/956841.pdf
Data publikacji:: 2017
Wydawca:: Polska Akademia Nauk. Instytut Dendrologii PAN
Tematy:: somatic embryogenesis
osmotic stress
germination
norway spruce
serbian spruce
Opis:: The objective of the presented study was to compare the effect of osmotic stress, induced by various concentrations of polyethylene glycol (PEG) MW 4000, on the development, maturation, and conversion of somatic embryos of Picea abies (model species) and P. omorika (endemic species) into somatic seedlings. Embryogenic tissue (ET) of P. abies (line A and B) and P. omorika were incubated on a maturation medium with 20 μM abscisic acid (ABA), 1 μM indole-3-butyric acid (IBA) and PEG 4000 at 0, 5, and 7.5%. After 5 weeks, the number of somatic embryos produced, and the number of cotyledonary somatic embryos per 1 gram of ET, was assessed. Cotyledonary somatic embryos were selected and transferred to a Margara germination medium for two months. Their growth, as measured by hypocotyl and radicle length, was evaluated after one month, and the somatic embryos were transferred to fresh medium. After the second month of incubation, the conversion frequency of germinated embryos into the seedlings was assessed. In general, ETs of both of the tested spruce species cultured on a maturation medium amended with 5% PEG 4000 exhibited a tendency to produce a higher total number of embryos, as well as a higher number of cotyledonary embryos. The B line of P. abies produced a significantly greater number of cotyledonary embryos, relative to the control (no PEG). PEG only stimulated hypocotyl growth in embryos of both lines (A and B) of P. abies during the germination stage. Somatic embryos of Picea omorika were characterized by poor growth of both hypocotyls and radicles, irrespective of PEG treatment. The addition of PEG 4000 to the maturation medium did not improve the conversion frequency of germinated somatic embryos into somatic seedlings in either spruce species after a month of incubation on Margara medium. The effect of PEG 4000 on somatic embryogenesis efficiency was more evident in the ET of the two P. abies lines, relative to the P. omorika ET line, during both the maturation and the germination stage. Results indicated that the P. omorika ET line was almost completely insensitive to all of the PEG concentrations tested.
Źródło:: Dendrobiology; 2017, 78; 168-178
1641-1307
Pojawia się w:: Dendrobiology
Dostawca treści:: Biblioteka Nauki

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