Autor: Wasik, Anita - Katalog OPAC zbiorów

Skocz do pozycji: 1.

Tytuł:: Społeczeństwo – tożsamości – obrazy. Kobieta i mężczyzna w czasie pracy i czasie wolnym w przedstawieniach saotomejskiego malarstwa reklamowego
Society – Identities – Images. The Feminine and the Masculine During Work and Leisure on São Toméan Advertisement Painting Images
Autorzy:: Wasik, Anita
Stańczyk, Piotr
Powiązania:: https://bibliotekanauki.pl/articles/522778.pdf
Data publikacji:: 2017
Wydawca:: Uniwersytet Warszawski. Katedra Języków i Kultur Afryki. Polskie Towarzystwo Afrykanistyczne
Tematy:: visual discourse analysis
visual semiotics
São Tomé
advertisement painting
popular art
visual design
work
free time
identity
Opis:: The main aim of this article is to present the results of research of the São Toméan advertisement painting phenomenon. Visual discourse analysis methods were used during the research procedure. The first part of the article presents various types of research on advertisement painting and the basic characteristics of this phenomenon in West Africa. The next part of the article describes the empirical methods used to analyse the visual discourse of São Toméan advertisement painting. The main part of the text presents an analysis of empirical visual material focused on the discursive construction of identity strategies towards production and work, leisure and consumption, as well as gender.
Źródło:: Afryka; 2017, 46; 95-116
1234-0278
Pojawia się w:: Afryka
Dostawca treści:: Biblioteka Nauki

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Skocz do pozycji: 2.

Tytuł:: Comparison of the utility of five commercial kits for extraction of DNA from Aspergillus fumigatus spores
Autorzy:: Nawrot, Urszula
Wlodarczyk, Katarzyna
Wrobel, Magdalena
Wasik, Anita
Dobosz, Tadeusz
Powiązania:: https://bibliotekanauki.pl/articles/1040325.pdf
Data publikacji:: 2010
Wydawca:: Polskie Towarzystwo Biochemiczne
Tematy:: DNA extraction
aspergillosis
Aspergillus
Opis:: The aim of this study was to compare the efficiency of DNA extraction from water as well as from blood samples spiked with A. fumigatus spores, using selected commercial kits. Extraction of DNA according to manufacturer's protocols was preceded by blood cells lysis and disruption of fungal cells by enzymatic digestion or bead beating. The efficiency of DNA extraction was measured by PCR using Aspergillus-specific primers and SYBR Green I dye or TaqMan probes targeting 28S rRNA gene. All methods allowed the detection of Aspergillus at the lowest tested density of water suspensions of spores (101 cells/ml). The highest DNA yield was obtained using the ZR Fungal/Bacterial DNA kit, YeastStar Genomic DNA kit, and QIAamp DNA Mini kit with mechanical cell disruption. The ZR Fungal/Bacterial DNA and YeastStar kits showed the highest sensitivity in examination of blood samples spiked with Aspergillus (100 % for the detection of 102 spores and 75 % for 101 spores). Recently, the enzymatic method ceased to be recommended for examination of blood samples for Aspergillus, thus ZR Fungal/Bacterial DNA kit and QIAamp DNA Mini kit with mechanical cell disruption could be used for extraction of Aspergillus DNA from clinical samples.
Źródło:: Acta Biochimica Polonica; 2010, 57, 4; 567-571
0001-527X
Pojawia się w:: Acta Biochimica Polonica
Dostawca treści:: Biblioteka Nauki

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Skocz do pozycji: 3.

Tytuł:: Metabolic transformations of antitumor imidazoacridinone, C-1311, with microsomal fractions of rat and human liver
Autorzy:: Wiśniewska, Anita
Chrapkowska, Agnieszka
Kot-Wasik, Agata
Konopa, Jerzy
Mazerska, Zofia
Powiązania:: https://bibliotekanauki.pl/articles/1040879.pdf
Data publikacji:: 2007
Wydawca:: Polskie Towarzystwo Biochemiczne
Tematy:: antitumor drug
imidazoacridinone
C-1311
P450 isoenzymes
microsomes
Symadex
in vitro metabolism
Opis:: The imidazoacridinone derivative C-1311 is an antitumor agent in Phase II clinical trials. The molecular mechanism of enzymatic oxidation of this compound in a peroxidase model system was reported earlier. The present studies were performed to elucidate the role of rat and human liver enzymes in metabolic transformations of this drug. C-1311 was incubated with different fractions of liver cells and the reaction mixtures were analyzed by RP-HPLC. We showed that the drug was more sensitive to metabolism with microsomes than with cytosol or S9 fraction of rat liver cells. Incubation of C-1311 with microsomes revealed the presence of four metabolites. Their structures were identified as dealkylation product, M0, as well as a dimer-like molecule, M1. Furthermore, we speculate that the hydroxyl group was most likely substituted in metabolite M3. It is of note that a higher rate of transformation was observed for rat than for human microsomes. However, the differences in metabolite amounts were specific for each metabolite. The reactivity of C-1311 with rat microsomes overexpressing P450 isoenzymes, of CYP3A and CYP4A families was higher than that with CYP1A and CYP2B. Moreover, the M1 metabolite was selectively formed with CYP3A, whereas M3 with CYP4A. In conclusion, this study revealed that C-1311 varied in susceptibility to metabolic transformation in rat and human cells and showed selectivity in the metabolism with P450 isoenzymes. The obtained results could be useful for preparing the schedule of individual directed therapy with C-1311 in future patients.
Źródło:: Acta Biochimica Polonica; 2007, 54, 4; 831-838
0001-527X
Pojawia się w:: Acta Biochimica Polonica
Dostawca treści:: Biblioteka Nauki

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