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Tytuł:
Calculation of laser radar cross-section of a spatial object and its experimental verification
Autorzy:
Cao, Y.-H.
Wang, Z.
Bai, L.
Wu, Z.
Li, H.-Y.
Li, Y.-H.
Powiązania:
https://bibliotekanauki.pl/articles/174167.pdf
Data publikacji:
2017
Wydawca:
Politechnika Wrocławska. Oficyna Wydawnicza Politechniki Wrocławskiej
Tematy:
bidirectional reflectance distribution function
BRDF
laser radar cross-section
LRCS
spatial object
Opis:
Bidirectional reflectance distribution function of some artificial satellite used materials at the wavelength of 1.06 μm was measured in laboratory. Bidirectional reflectance distribution function (BRDF) models of these materials were established with the five-parameter BRDF model. Laser radar cross-section of a scaled satellite with various materials was calculated, and characteristics of laser radar cross-section of the satellite were discussed. Measurement system to measure laser radar cross-section of the satellite was established and the scaled satellite model was measured. By analyzing the measured data and calculated data, it can be clearly seen that the BRDF of the surface materials and the laser incidence angle are two of main influential factors of the scaled satellite’s laser radar cross-section. These works can provide a reference for design of the lidar system.
Źródło:
Optica Applicata; 2017, 47, 4; 521-532
0078-5466
1899-7015
Pojawia się w:
Optica Applicata
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Badania okresu gwarancyjnego dla systemu wieloskładnikowego, w którym zachodzą interakcje uszkodzeniowe
Research on warranty interval of multi-component system with failure interaction
Autorzy:
Cheng, Z. H.
Bai, Y. S.
Cai, L. Y.
Wang, L. Ch.
Li, P. J.
Chen, L.
Powiązania:
https://bibliotekanauki.pl/articles/301278.pdf
Data publikacji:
2011
Wydawca:
Polska Akademia Nauk. Polskie Naukowo-Techniczne Towarzystwo Eksploatacyjne PAN
Tematy:
okres gwarancyjny
koszty
dostępność
interakcje uszkodzeniowe
wieloelementowy
warranty period
cost
availability
failure interaction
multi-component
Opis:
W oparciu o analizę interakcji uszkodzeniowych, przyjęto dla systemu wieloskładnikowego politykę gwarancyjną obejmującą niepełną odnowę profilaktyczną. Zbadano średnią intensywność uszkodzeń dla każdego okresu gwarancyjnego oraz skonstruowano modele kosztów obsługi gwarancyjnej oraz dostępności biorąc pod uwagę intensywność uszkodzeń interakcyjnych. Jako przykład podano projekt okresu gwarancyjnego, który może potwierdzić poprawność przyjętego modelu oraz przedstawiono zalety takiego projektu. W badaniach opracowano technikę i metody ustalania okresu gwarancyjnego dla systemów wieloskładnikowych, które stanowią istotny wkład do teorii gwarancji.
Based on the analysis of failure interaction, imperfect preventive warranty policy is adopted for the multi-component system. Average failure rate of each warranty interval is studied and warranty cost model and availability model are built as viewed from interactive failure rate. Then Warranty period project is brought forward as an example, which can validate the feasibility of model and show the advantage of the project. The research can provide technique and methods for determining Warranty Period of multi-component system, which further enriches and perfects the warranty theory.
Źródło:
Eksploatacja i Niezawodność; 2011, 4; 49-55
1507-2711
Pojawia się w:
Eksploatacja i Niezawodność
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
A super depth of field height measurement based on local disparity
Autorzy:
Cui, L.
Liu, Y.
Yu, M.
Jiang, G.
Fan, S.
Wang, Y.
Powiązania:
https://bibliotekanauki.pl/articles/173522.pdf
Data publikacji:
2015
Wydawca:
Politechnika Wrocławska. Oficyna Wydawnicza Politechniki Wrocławskiej
Tematy:
microscopes
binocular vision
local disparity map
depth of field (DOF)
height measurement
Opis:
A super depth of field height measurement method is proposed to measure the object height with the optical stereoscopic microscope. The quasi-Euclidean epipolar rectification algorithm is utilized on the original stereoimage to obtain rectified stereoimages and calibrate two camera parameters. Then, feature points are obtained by the SURF (speed up robust feature) algorithm and their corresponding disparities are calculated. The disparity-depth of field curve is fitted by combining the step height values of a stepper motor. Moreover, through local disparity value got from feature points on the object, the relative shift height is calculated through regression analysis. Finally, according to binocular vision geometry, the thickness of the object can be calculated. Experimental results show that the measurement error in Z direction is from 1.51% to 7.71%, which indicates that the proposed method is able to measure the height of a microobject beyond depth of field within a tolerant error.
Źródło:
Optica Applicata; 2015, 45, 2; 205-214
0078-5466
1899-7015
Pojawia się w:
Optica Applicata
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Research on rub-impact loads response of ship shafting
Autorzy:
Dong, L.
Shi, Y.
Wang, S.
Powiązania:
https://bibliotekanauki.pl/articles/260309.pdf
Data publikacji:
2018
Wydawca:
Politechnika Gdańska. Wydział Inżynierii Mechanicznej i Okrętownictwa
Tematy:
rub-impact
Torsional Vibration
Test Rig
Rubbing Generator
Opis:
The anti-impact ability of shafting affects stability and security of the ship power transmission directly. Moreover, it also cannot be ignored that the rub-impact loads have influence on the torsion vibration of ship shafting. In order to solve the problem of engineering application of reliability assessment under rub-impact loads, a test rig with rubbing generator is established. By carrying out the integrative analysis, the torsional vibration characteristics, such as vibration amplitude and orbit of axle center under the rub impact load are studied. According to the rub-impact conditions obtained through numerical simulation, the experimental verification is carried out on the test rig with rubbing generator. The results show that it is not obvious the influence of rub-impact loads upon the shafting torsion vibration except in special working conditions, that can be simulated by the rubbing generator. The maximum amplitude of torsional vibration is influenced by the radial rigidity as well as the friction coefficient of rubbing body, and the degree of influence is difference under conditions of continuous rubbing and serious rubbing. By adjusting the rigidity of stern bearing, the influence of rub-impact upon shafting can be weaken, which provides a theoretical reference for the safety evaluation of ship shafting.
Źródło:
Polish Maritime Research; 2018, S 2; 85-91
1233-2585
Pojawia się w:
Polish Maritime Research
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Serological and molecular evidence of Coxiella burnetii in samples from humans and animals in China
Autorzy:
El-Mahallawy, H.S.
Kelly, P.
Zhang, J.
Yang, Y.
Wei, L.
Tian, L.
Fan, W.
Zhang, Z.
Wang, C.
Powiązania:
https://bibliotekanauki.pl/articles/49293.pdf
Data publikacji:
2016
Wydawca:
Instytut Medycyny Wsi
Źródło:
Annals of Agricultural and Environmental Medicine; 2016, 23, 1
1232-1966
Pojawia się w:
Annals of Agricultural and Environmental Medicine
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Cold rolling mill thickness control using the cascade-correlation neural network
Autorzy:
Frayman, Y.
Wang, L.
Wan, C.
Powiązania:
https://bibliotekanauki.pl/articles/206844.pdf
Data publikacji:
2002
Wydawca:
Polska Akademia Nauk. Instytut Badań Systemowych PAN
Tematy:
konstrukcja dynamicznej sieci neuronowej
sieć neuronowa kaskadowo-korelacyjna
sterowanie grubością walcowni zimnej
cascade-correlation neural network
cold rolling mill thickness control
direct MRAC
dynamic neural network construction
Opis:
The improvements in thickness accuracy of a steel strip produced by a tandem cold-rolling mill are of substantial interest to the steel industry. In this paper, we designed a direct model-reference adaptive control (MRAC) scheme that exploits the natural level of excitation existing in the closed-loop with a dynamically constructed cascade-correlation neural network (CCNN) as a controller for cold rolling mill thickness control. Simulation results show that the combination of a such a direct MRAC scheme and the dynamically constructed CCNN significantly improves the thickness accuracy in the presence of disturbances and noise in comparison with to the conventional PID controllers.
Źródło:
Control and Cybernetics; 2002, 31, 2; 327-342
0324-8569
Pojawia się w:
Control and Cybernetics
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Pretreatment of coking wastewater by an adsorption process using fine coking coal
Autorzy:
Gao, L.
Li, S.
Wang, Y.
Gui, X.
Xu, H.
Powiązania:
https://bibliotekanauki.pl/articles/110417.pdf
Data publikacji:
2016
Wydawca:
Politechnika Wrocławska. Oficyna Wydawnicza Politechniki Wrocławskiej
Tematy:
coking coal
coking wastewater
adsorption
COD
phenol
Opis:
A new technique for pretreatment of coking wastewater is introduced based on the concept of circular economy. Coal is fed into a coking system after adsorption. This study validates the feasibility of using coking coal to adsorb organic pollutants in coking wastewater. The sorption kinetics and equilibrium sorption isotherms of coking coal for removal of chemical oxygen demand (COD) and phenol from coking wastewater was also discussed in this paper. Gas chromatograph/mass spectroscopy (GC/MS) was used to detect changes in the quality of coking wastewater. The results showed that when coking coal dosage was 120 g/dm3, 65% of COD and 34% of phenol in waste water can be removed after 40 min of agitation. The surface functional groups of coking coal before and after adsorption were observed with a Fourier transform infrared spectrometer. The kinetics of COD and phenol adsorption from coking wastewater by coking coal fitted the pseudo second-order model. The adsorption process of coking coal can be classified into two categories, namely, rapid and slow. The Freundlich isotherm provided a better fit with all adsorption isotherms than the Langmuir isotherm. Coking coal could be a suitable low-cost adsorbent for recalcitrant organic pollutants.
Źródło:
Physicochemical Problems of Mineral Processing; 2016, 52, 1; 422-436
1643-1049
2084-4735
Pojawia się w:
Physicochemical Problems of Mineral Processing
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Optimal combinatorial replacement policy under a given maintenance interval for the combined governor in diesel locomotives
Optymalna strategia wymiany kombinatorycznej dla danej częstotliwości przeglądów regulacji mieszanej w lokomotywach spalinowych
Autorzy:
Gao, W.
Zhang, Z.
Zhou, Y.
Wang, R.
Jiang, L.
Powiązania:
https://bibliotekanauki.pl/articles/302197.pdf
Data publikacji:
2013
Wydawca:
Polska Akademia Nauk. Polskie Naukowo-Techniczne Towarzystwo Eksploatacyjne PAN
Tematy:
modele niezawodności
rozkład Weibulla
strategia wymiany kombinatorycznej
lokomotywa spalinowa o mieszanej regulacji
reliability models
mixed-Weibull distribution
combinatorial replacement policy
combined governor of diesel locomotives
Opis:
Combined governor is one of key components in diesel locomotives, as a subcomponent it must meet the existing maintenance periodic of the diesel locomotive, while it is passively replaced or maintained in a midway because of over/under-maintenance in practice. In this paper, four reliability models of a sequential PM cycle are developed using three years of maintenance data of combined governors in one Chinese Railway Bureau to determine its reliability distribution, in which some zero-failure data and censor data are used. Meanwhile, a novel combinatorial replacement (CR) policy is proposed to optimize its preventive maintenance (PM), in which a component is replaced several times using a preventively maintained one in a given operational interval. After that, necessary optimizations are introduced based on the determined reliability models and the maintenance interval given by the PM criterion of diesel locomotives (23000km ~ 25000km), and then the genetic algorithm is also used to solve the constraint optimization function. Results show that the proposed CR policy is the best policy among the existing policy and the general (T, δ) policy, and other results can be viewed as an optional policy when spare components are limited.
Regulacja mieszana jest jednym z kluczowych elementów w lokomotywach spalinowych, i jako taka musi być ujęta w istniejącym systemie konserwacji okresowej lokomotyw spalinowych. W praktyce jednak, podlega ona biernej wymianie lub przedwczesnej konserwacji z powodu niewystarczających lub nadmiernych praktyk utrzymania w ruchu. W niniejszym artykule opracowano cztery modele niezawodnościowe sekwencyjnego cyklu konserwacji zapobiegawczej z wykorzystaniem danych z trzech lat konserwacji mieszanej regulacji jednego Biura Kolei Chińskich w celu ustalenia ich rozkładu niezawodności, przy użyciu wybranych danych nt. nieuszkadzalności oraz danych cenzurowanych. W celu optymalizacji konserwacji zapobiegawczej (PM), zaproponowano nową strategię wymiany kombinatorycznej (combinatorial replacement - CR), w której element składowy zostaje kilkakrotnie zastąpiony innym elementem uprzednio poddanym konserwacji zapobiegawczej w danym okresie eksploatacyjnym. Następnie, wprowadzono konieczne optymalizacje na podstawie opracowanych modeli niezawodnościowych oraz częstotliwości przeglądów podanej w kryteriach konserwacji zapobiegawczej lokomotyw spalinowych (23000 km ~ 25000 km). W dalszej kolejności, wykorzystano algorytm genetyczny do rozwiązania funkcji optymalizacji ograniczeń. Wyniki pokazują, że proponowana strategia CR jest najlepsza spośród istniejących strategii i ogólnych strategii (T, δ); inne wyniki można traktować jako strategię opcjonalną w sytuacji gdy dostępność elementów zamiennych jest ograniczona.
Źródło:
Eksploatacja i Niezawodność; 2013, 15, 2; 89-98
1507-2711
Pojawia się w:
Eksploatacja i Niezawodność
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Auto mission planning system design for imaging satellites and its applications in environmental field
Autorzy:
He, Y.
Wang, Y.
Chen, Y.
Xing, L.
Powiązania:
https://bibliotekanauki.pl/articles/258754.pdf
Data publikacji:
2016
Wydawca:
Politechnika Gdańska. Wydział Inżynierii Mechanicznej i Okrętownictwa
Tematy:
systems engineering
imaging satellite
meteorological monitoring
space-ground integration
Opis:
Satellite hardware has reached a level of development that enables imaging satellites to realize applications in the area of meteorology and environmental monitoring. As the requirements in terms of feasibility and the actual profit achieved by satellite applications increase, we need to comprehensively consider the actual status, constraints, unpredictable information, and complicated requirements. The management of this complex information and the allocation of satellite resources to realize image acquisition have become essential for enhancing the efficiency of satellite instrumentation. In view of this, we designed a satellite auto mission planning system, which includes two sub-systems: the imaging satellite itself and the ground base, and these systems would then collaborate to process complicated missions: the satellite mainly focuses on mission planning and functions according to actual parameters, whereas the ground base provides auxiliary information, management, and control. Based on the requirements analysis, we have devised the application scenarios, main module, and key techniques. Comparison of the simulation results of the system, confirmed the feasibility and optimization efficiency of the system framework, which also stimulates new thinking for the method of monitoring environment and design of mission planning systems.
Źródło:
Polish Maritime Research; 2016, S 1; 59-70
1233-2585
Pojawia się w:
Polish Maritime Research
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Vibration analysis of ball bearing considering waviness under high speed and an axial load
Autorzy:
Hou, P. P.
Wang, L. Q.
Peng, Q. Y.
Powiązania:
https://bibliotekanauki.pl/articles/201434.pdf
Data publikacji:
2020
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
rotational speed
ball bearing
vibration characteristics
frequency band
RMS
Opis:
Based on the rolling bearing vibration measurement principle in ISO standard, a nonlinear dynamic model of ball bearing is built and motion equations of the inner ring, outer ring, and rolling elements are derived by using Lagrange’s equation. The ball bearing model includes the influence of waviness, rotational speed, external load, arbor supporting stiffness and arbor eccentricity. Ball bearing high-speed vibration tests are performed and used to verify the theoretical results. Simulated results showed that specific waviness orders produced the principal frequencies that were proportional to rotational speed. Rotational speed mainly affected the value of the natural frequency of the bearing system, and RMS (Root Mean Square) of the full band had a great fluctuation with the increase of rotational speed. In the experiment, spectrum and RMS of 2 ƒs-30 kHz (ƒs : the rotational frequency of inner ring/arbor) under high speed could include not only the influence of rotational speed but also principal frequencies produced by waviness, which could cover the part of requirements of the standard bearing vibration measurement.
Źródło:
Bulletin of the Polish Academy of Sciences. Technical Sciences; 2020, 68, 3; 517-527
0239-7528
Pojawia się w:
Bulletin of the Polish Academy of Sciences. Technical Sciences
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Influence of selenium on growth, lipid peroxidation and antioxidative enzyme activity in melon (Cucumis melo L.) seedlings under salt stress
Autorzy:
Hu, K.L.
Zhang, L.
Wang, J.T.
You, Y.
Powiązania:
https://bibliotekanauki.pl/articles/58727.pdf
Data publikacji:
2013
Wydawca:
Polskie Towarzystwo Botaniczne
Tematy:
selenium
plant growth
lipid peroxidation
antioxidative enzyme
enzyme activity
melon
Cucumis melo
seedling
salt stress
Opis:
The objective of this study was to investigate the effect of exogenous selenium (Se) supply (0, 2, 4, 8, 16 μM) on the growth, lipid peroxidation and antioxidative enzyme activity of 100 mM NaCl-stressed melon (Cucumis melo L.) seedlings. Salt stress significantly reduced the growth attributes including stem length, stem diameter, dry weight and increased antioxidative enzyme activity [superoxide dismutase (SOD), peroxidase (POD), catalase (CAT)]. Moreover, the plant exhibited a significant increase in electrolyte leakage and malondialdehyde (MDA) content under NaCl stress. Se supplementation not only improved the growth parameters but also successfully ameliorated the adverse effect caused by salt stress in melon seedlings. However, the mitigation of NaCl-stressed seedlings was different depending on the Se concentration. At lower concentrations (2–8 μM), Se improved growth and acted as antioxidant by inhibiting lipid peroxidation and increasing in SOD and POD enzymes activity under salt stress. At higher concentrations (16 μM), Se exerted diminished beneficial effects on growth. Whereas CAT activity was enhanced. The result indicated that Se supplementation had a positive physiological effect on the growth and development of salt-stressed melon seedlings.
Źródło:
Acta Societatis Botanicorum Poloniae; 2013, 82, 3
0001-6977
2083-9480
Pojawia się w:
Acta Societatis Botanicorum Poloniae
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
A multiplex PCR for simultaneous detection of classical swine fever virus, African swine fever virus, highly pathogenic porcine reproductive and respiratory syndrome virus, porcine reproductive and respiratory syndrome virus and pseudorabies in swines
Autorzy:
Hu, L.
Lin, X.Y.
Yang, Z.X.
Yao, X.P.
Li, G.L.
Peng, S.Z.
Wang, Y.
Powiązania:
https://bibliotekanauki.pl/articles/30249.pdf
Data publikacji:
2015
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Opis:
In this assay, we developed and evaluated a multiplex PCR (mPCR) for its ability in detecting multiple infections of swine simultaneously. Four pairs of primers were used to detect five viruses. Specific primers were designed for classical swine fever virus (CSFV), African swine fever virus (ASFV) and pseudorabies (PRV). A pair of primers was designed prudently for two different types of porcine reproductive and respiratory syndrome virus that respectively were porcine reproductive and respiratory syndrome virus (PRRSV), highly pathogenic porcine reproductive and respiratory syndrome virus (HP-PRRSV). The detection limits of the mPCR were 1.09×10⁴, 1.50×10³, 2.10×10³, 1.30×10³ and 8.97×10² copies/reaction for CSFV, ASFV, HP-PRRSV, PRRSV and PRV, respectively. A total of 49 clinical specimens were tested by the mPCR, and the result showed that co-infection by two or three viruses was 51%. In conclusion, the PCR is a useful tool for clinical diagnosis of not only single infections but also mixed infections in swines.
Źródło:
Polish Journal of Veterinary Sciences; 2015, 18, 4
1505-1773
Pojawia się w:
Polish Journal of Veterinary Sciences
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Effect of overlying water pH, dissolved oxygen and temperature on heavy metal release from river sediments under laboratory conditions
Autorzy:
Huang, Y.
Zhang, D.
Xu, Z.
Yuan, S.
Li, Y.
Wang, L.
Powiązania:
https://bibliotekanauki.pl/articles/204852.pdf
Data publikacji:
2017
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
heavy metal
release
river sediment
Opis:
The heavy metal release experiments were conducted in the laboratory to examine the effects of 3 factors - pH, dissolved oxygen (DO), and temperature on the metal release from sediments taken from the Huangpu River. The metal concentrations in the dry sediments ranged from 0.030 to 0.296 mg g-1 for Cr, 0.021 to 0.097 mg g-1 for Ni, 0.014 to 0.219 mg g-1 for Cu, 0.035 mg to 0.521 mg g-1 for Zn, 0.0002 to 0.001 mg g-1 for Cd and 0.023 to 0.089 mg g-1 for Pb. Most of the metals found in the sediments were in the form of residual fraction, the exchangeable fraction consisted of only a small portion of total metals. The average dissolved metal concentrations in the overlying water during the 13-day period under different conditions were ranging from 0.82 to 1.93 μg L-1 for Cr, 1.08 to 4.19 μg L-1 for Ni, 40.79 to 82.28 μg L-1 for Cu, 20.30 to 29.96 μg L-1 for Zn, 1.57 to 4.07 μg L-1 for Cd, and 22.26 to 75.50 μg L-1 for Pb, respectively. Statistical interpretation of the data indicated that pH (7, 8, 9), dissolved oxygen DO (1.0 and 5.0 mg L-1) and temperature (4, 16, 25°C) had no significant effects on the heavy metal release under the studied conditions. Cu and Pb had the highest release flux, while Cd, Pb and Cu had higher mobility. The main factors controlling the metals release might be the inherent characters of metals and sediments.
Źródło:
Archives of Environmental Protection; 2017, 43, 2; 28-36
2083-4772
2083-4810
Pojawia się w:
Archives of Environmental Protection
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Self-Plasticization of PVC via click reaction of a monooctyl phthalate derivative
Autorzy:
Jia, P.
Wang, R.
Hu, L.
Zhang, M.
Zhou, Y.
Powiązania:
https://bibliotekanauki.pl/articles/779324.pdf
Data publikacji:
2017
Wydawca:
Zachodniopomorski Uniwersytet Technologiczny w Szczecinie. Wydawnictwo Uczelniane ZUT w Szczecinie
Tematy:
poly(vinyl chloride)
plasticization
migration
TGA
Opis:
Modified PVC (M-PVC) material with suppressed migration and low glass transition temperature was prepared via click reaction of a monooctyl phthalate derivative. Chemical structure and composition of M-PVC were characterized by FT-IR, 1H NMR and element analysis. Thermal stability, glass transition temperature and migration stability of M-PVC were studied with TGA, DSC and migration tests, respectively. The study showed that M-PVC exhibited poor thermal stability, and low glass transition temperature of 66.0°C. No migration was found in distilled water, 10% (v/v) ethanol, 30% (w/v)acetic acid and petroleum ether. The PVC material is expected to preparing PVC products in the areas with high migration resistance requirement.
Źródło:
Polish Journal of Chemical Technology; 2017, 19, 3; 16-19
1509-8117
1899-4741
Pojawia się w:
Polish Journal of Chemical Technology
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Rapid screening of monoclonal antibodies against porcine circovirus type 2 using colloidal gold-based paper test
Autorzy:
Jin, Q.Y.
Feng, L.L.
Wang, Y.B.
Li, P.
Yang, J.F.
Teng, M.
Chai, S.J.
Xing, G.X.
Zhang, G.P.
Powiązania:
https://bibliotekanauki.pl/articles/16539073.pdf
Data publikacji:
2022
Wydawca:
Polska Akademia Nauk. Czasopisma i Monografie PAN
Tematy:
porcine circovirus type 2 (PCV2)
screening of MAbs
paper test
Opis:
A proof of concept for using paper test as a suitable method in the production of monoclonal antibodies (MAbs) is reported. The paper test which detects antibodies against porcine circovirus type 2 (PCV2) using colloidal gold-labelled capsid protein as the antigen probe was applied exclusively in the screening of anti-PCV2 MAbs. It allowed the detection of 118 single cell clones within 30 min using naked eyes. MAbs with specific binding to authentic epitopes on the virus were selected using a blocking strategy in which the antibody was pre-incubated with PCV2 viral sample before applying to the test paper. Five hybridomas secreting MAbs against the capsid protein were obtained, with only three of them capable of binding to PCV2. The results were validated and confirmed using enzyme-linked immunosorbent assay and immunofluorescence assay. The paper test is simple, rapid, and independent on professional technicians and proves to be an excellent approach for the screening of MAbs against specific targets.
Źródło:
Polish Journal of Veterinary Sciences; 2022, 25, 1; 27-34
1505-1773
Pojawia się w:
Polish Journal of Veterinary Sciences
Dostawca treści:
Biblioteka Nauki
Artykuł
Wyświetlanie 1-15 z 96

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