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Wyświetlanie 1-15 z 35
Tytuł:
Rapid screening of monoclonal antibodies against porcine circovirus type 2 using colloidal gold-based paper test
Autorzy:
Jin, Q.Y.
Feng, L.L.
Wang, Y.B.
Li, P.
Yang, J.F.
Teng, M.
Chai, S.J.
Xing, G.X.
Zhang, G.P.
Powiązania:
https://bibliotekanauki.pl/articles/16539073.pdf
Data publikacji:
2022
Wydawca:
Polska Akademia Nauk. Czasopisma i Monografie PAN
Tematy:
porcine circovirus type 2 (PCV2)
screening of MAbs
paper test
Opis:
A proof of concept for using paper test as a suitable method in the production of monoclonal antibodies (MAbs) is reported. The paper test which detects antibodies against porcine circovirus type 2 (PCV2) using colloidal gold-labelled capsid protein as the antigen probe was applied exclusively in the screening of anti-PCV2 MAbs. It allowed the detection of 118 single cell clones within 30 min using naked eyes. MAbs with specific binding to authentic epitopes on the virus were selected using a blocking strategy in which the antibody was pre-incubated with PCV2 viral sample before applying to the test paper. Five hybridomas secreting MAbs against the capsid protein were obtained, with only three of them capable of binding to PCV2. The results were validated and confirmed using enzyme-linked immunosorbent assay and immunofluorescence assay. The paper test is simple, rapid, and independent on professional technicians and proves to be an excellent approach for the screening of MAbs against specific targets.
Źródło:
Polish Journal of Veterinary Sciences; 2022, 25, 1; 27-34
1505-1773
Pojawia się w:
Polish Journal of Veterinary Sciences
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Uniformity of electroelastic field within a three-phase anisotropic piezoelectric elliptical inhomogeneity in anti-plane shear
Autorzy:
Wang, X.
Schiavone, P.
Powiązania:
https://bibliotekanauki.pl/articles/38694715.pdf
Data publikacji:
2022
Wydawca:
Instytut Podstawowych Problemów Techniki PAN
Tematy:
three-phase elliptical inhomogeneity
confocal ellipses
monoclinic piezoelectric material
transversely isotropic piezoelectric material
Stroh quartic formalism
real-form solution
Opis:
Using the Stroh quartic formalism, we prove that the internal electroelastic field is unconditionally uniform inside a three-phase anisotropic piezoelectric elliptical inhomogeneity with two confocal elliptical interfaces when the surrounding matrix is subjected to uniform remote anti-plane mechanical and in-plane electrical loading. The inhomogeneity and the matrix comprise monoclinic piezoelectric materials with symmetry plane at x3 = 0 and with poling in the x3-direction; the intermediate interphase layer is a transversely isotropic piezoelectric material with poling in the x3-direction. Moreover, we obtain the internal uniform electroelastic field inside the elliptical inhomogeneity and the non-uniform electroelastic field in the interphase layer in real-form in terms of the fundamental piezoelectricity matrices for both the inhomogeneity and the matrix and the generalized Barnett–Lothe tensors for both the interphase layer and the matrix.
Źródło:
Archives of Mechanics; 2022, 74, 2-3; 143-156
0373-2029
Pojawia się w:
Archives of Mechanics
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
A screw dislocation located outside, inside or on the interface of a parabolic elastic inhomogeneity
Autorzy:
Wang, X.
Schiavone, P.
Powiązania:
https://bibliotekanauki.pl/articles/38627364.pdf
Data publikacji:
2021
Wydawca:
Instytut Podstawowych Problemów Techniki PAN
Tematy:
screw dislocation
parabolic elastic inhomogeneity
superposition
conformal mapping
analytic continuation
Opis:
Using conformal mapping techniques, superposition and analytic continuation, we derive analytic solutions to the problem of a screw dislocation interacting with a parabolic elastic inhomogeneity. The screw dislocation can be located anywhere either in the surrounding matrix or in the parabolic inhomogeneity or simply on the parabolic interface itself. We obtain explicit expressions for the two analytic functions in the image plane characterizing the elastic fields describing displacement and stresses in the two-phase composite. Using the Peach-Koehler formula, we also obtain the image force acting on the screw dislocation. The analytic function defined in the parabolic inhomogeneity in the physical plane can be interpreted in terms of real and image screw dislocations for any location of the real screw dislocation.
Źródło:
Archives of Mechanics; 2021, 73, 3; 219-235
0373-2029
Pojawia się w:
Archives of Mechanics
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Antibiofilm activity against Staphylococcus aureus and content analysis of Taraxacum Officinale phenolic extract
Autorzy:
Xu, P.
Xu, X.B.
Khan, A.
Fotina, T.
Wang, S.H.
Powiązania:
https://bibliotekanauki.pl/articles/2087162.pdf
Data publikacji:
2021
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
Taraxacum Officinale
phenolic
antibacterial mechanism
HPLC
Staphylococcus aureus
Źródło:
Polish Journal of Veterinary Sciences; 2021, 24, 2; 243-251
1505-1773
Pojawia się w:
Polish Journal of Veterinary Sciences
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Development of a SYBR Green I real-time PCR assay for detection of novel porcine parvovirus 7
Autorzy:
Li, Y.D.
Yu, Z.D.
Bai, C.X.
Zhang, D.
Sun, P.
Peng, M.L
Liu, H.
Wang, J.
Wang, Y.
Powiązania:
https://bibliotekanauki.pl/articles/2087207.pdf
Data publikacji:
2021
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
Capsid gene
PPV7
SYBR Green I real-time PCR
Źródło:
Polish Journal of Veterinary Sciences; 2021, 24, 1; 43-49
1505-1773
Pojawia się w:
Polish Journal of Veterinary Sciences
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
The Bending, Impact Fracture Behavior and Characteristics of Stainless Steel Clad Plates with Different Rolling Temperature
Autorzy:
An, Q.
Fan, K. Y.
Ge, Y. F.
Liu, B. X.
He, J.
Wang, S.
Chen, C. X.
Ji, P. G.
Tolochko, O.
Powiązania:
https://bibliotekanauki.pl/articles/2049433.pdf
Data publikacji:
2021
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
stainless steel clad plate
interface characteristics
bending behavior
impact morphologies
delamination crack
Opis:
The interface characteristics, bending and impact behavior, as well as fracture characteristics of stainless steel clad plates fabricated by vacuum hot rolling at different rolling temperatures of 1100°C, 1200°C and 1300°C are investigated in detail. The interface bonding strength is gradually increased with the increasing rolling temperature due to the sufficient diffusion behavior of alloy element. The bending toughness and impact toughness are gradually decreased, while the bending strength increase with the increase of the rolling temperature, which is attributed to mechanisms of matrix softening and interface strengthening at high rolling temperature. Due to the weak interface at 1100°C, the bending and impact crack propagation path was displaced by delamination cracks, which in turn lead to reduction in stress intensity of the main crack, playing an effective role in toughening the stainless steel clad plates. Moreover, the impact fracture morphologies of clad plates show a typical ductile-brittle transition phenomenon, which is attributed to the matrix softening behavior with the increasing rolling temperature.
Źródło:
Archives of Metallurgy and Materials; 2021, 66, 1; 229-239
1733-3490
Pojawia się w:
Archives of Metallurgy and Materials
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Uniform field within a non-elliptical inhomogeneity in the vicinity of a nearby non-circular Eshelby inclusion
Autorzy:
Wang, X.
Schiavone, P.
Powiązania:
https://bibliotekanauki.pl/articles/38629511.pdf
Data publikacji:
2021
Wydawca:
Instytut Podstawowych Problemów Techniki PAN
Tematy:
non-elliptical inhomogeneity
Booth’s lemniscate inclusion
uniform field
conformal mapping
anti-plane elasticity
inverse problem
Opis:
We rigorously prove that a non-elliptical inhomogeneity continues to permit an internal uniform stress field despite the presence of a nearby non-circular Eshelby inclusion undergoing uniform anti-plane eigenstrains when the surrounding matrix is subjected to uniform remote anti-plane stresses. Here, we adopt a specific representation of the non-circular Eshelby inclusion as a Booth’s lemniscate inclusion. Our analysis indicates that the internal uniform stress field inside the non-elliptical inhomogeneity is independent of the existence of the Booth’s lemniscate inclusion whereas the non-elliptical shape of the inhomogeneity is attributed entirely to its presence. Representative numerical examples are presented to demonstrate the feasibility of the proposed method of general solution.
Źródło:
Archives of Mechanics; 2021, 73, 5-6; 541-555
0373-2029
Pojawia się w:
Archives of Mechanics
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Uniform stress field inside a non-parabolic open inhomogeneity interacting with a mode III crack
Autorzy:
Wang, X.
Schiavone, P.
Powiązania:
https://bibliotekanauki.pl/articles/38612202.pdf
Data publikacji:
2021
Wydawca:
Instytut Podstawowych Problemów Techniki PAN
Tematy:
non-parabolic inhomogeneity
mode III crack
uniform stress field
antiplane elasticity
conformal mapping
singular integral equation
Opis:
Using conformal mapping techniques, analytic continuation and the theory of Cauchy singular integral equations, we prove that a non-parabolic open inhomogeneity embedded in an elastic matrix subjected to a uniform remote anti-plane stress nevertheless admits an internal uniform stress field despite the presence of a finite mode III crack in its vicinity. Our analysis indicates that: (i) the internal uniform stress field is independent of the specific shape of the inhomogeneity and the presence of the finite crack; (ii) the existence of the finite crack plays a key role in the non-parabolic open shape of the inhomogeneity and in the non-uniform stresses in the surrounding matrix; (iii) the two-term asymptotic expansion at infinity of the stress field in the matrix is independent of the presence of the finite crack. Detailed numerical results are presented to demonstrate the proposed theory.
Źródło:
Archives of Mechanics; 2021, 73, 1; 67-81
0373-2029
Pojawia się w:
Archives of Mechanics
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
17 beta-estradiol affects proliferation and apoptosis of canine bone marrow mesenchymal stem cells in vitro
Autorzy:
Zhou, Z.-H.
Gu, C.-W.
Li, J.
Huang, X.-Y.
Deng, J.-Q.
Shen, L.-H.
Cao, S.-Z.
Deng, J.-L.
Zuo, Z.-C.
Wang, Y.
Ma, X.-P.
Ren, Z.-H.
Yu, S.-M.
Powiązania:
https://bibliotekanauki.pl/articles/2087308.pdf
Data publikacji:
2020
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
apoptosis
bone marrow mesenchymal stem cells (BMSCs)
canine
proliferation
17 beta-estradiol (E2)
Źródło:
Polish Journal of Veterinary Sciences; 2020, 23, 2; 235-245
1505-1773
Pojawia się w:
Polish Journal of Veterinary Sciences
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
SYBR Green-based real-time polymerase chain reaction assay for detection of porcine parvovirus 6 in pigs
Autorzy:
Sun, P.
Bai, C.X.
Zhang, D.
Wang, J.
Yang, K.K.
Cheng, B.Z.
Li, Y.D.
Wang, Y.
Powiązania:
https://bibliotekanauki.pl/articles/2087328.pdf
Data publikacji:
2020
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
porcine parvovirus 6
real-time polymerase chain reaction
SYBR Green
Źródło:
Polish Journal of Veterinary Sciences; 2020, 23, 2; 197-202
1505-1773
Pojawia się w:
Polish Journal of Veterinary Sciences
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Three-phase parabolic inhomogeneities with internal uniform stresses in plane and anti-plane elasticity
Autorzy:
Wang, X.
Schiavone, P.
Powiązania:
https://bibliotekanauki.pl/articles/38442156.pdf
Data publikacji:
2020
Wydawca:
Instytut Podstawowych Problemów Techniki PAN
Tematy:
three-phase parabolic inhomogeneity
coating
internal uniform stresses
plane elasticity
anti-plane elasticity
Opis:
We examine the in-plane and anti-plane stress states inside a parabolic inhomogeneity which is bonded to an infinite matrix through an intermediate coating. The interfaces of the three-phase parabolic inhomogeneity are two confocal parabolas. The corresponding boundary value problems are studied in the physical plane rather than in the image plane. A simple condition is found that ensures that the internal stress state inside the parabolic inhomogeneity is uniform and hydrostatic. Furthermore, this condition is independent of the elastic properties of the coating and the two geometric parameters of the composite: in fact, the condition depends only on the elastic constants of the inhomogeneity and the matrix and the ratio between the two remote principal stresses. Once this condition is met, the mean stress in the coating is constant and the hoop stress on the coating side is also uniform along the entire inhomogeneity-coating interface. The unconditional uniformity of stresses inside a three-phase parabolic inhomogeneity is achieved when the matrix is subjected to uniform remote anti-plane shear stresses. The internal uniform anti-plane shear stresses inside the inhomogeneity are independent of the shear modulus of the coating and the two geometric parameters of the composite.
Źródło:
Archives of Mechanics; 2020, 72, 1; 27-38
0373-2029
Pojawia się w:
Archives of Mechanics
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
A polarization filter at 1550 nm based on photonic crystal fiber with symmetry around gold-coated holes
Autorzy:
Zha, F.
Li, J.
Sun, P.
Wang, X.
Powiązania:
https://bibliotekanauki.pl/articles/173897.pdf
Data publikacji:
2018
Wydawca:
Politechnika Wrocławska. Oficyna Wydawnicza Politechniki Wrocławskiej
Tematy:
photonic crystal fiber
polarization filter
finite element method
surface plasmon resonance
Opis:
A polarization photonic crystal fiber based on surface plasmon resonance is proposed in this paper. The photonic crystal fiber with gold coated holes is studied through using the finite element method. The impacts of structural parameters on the resonance characteristics are discussed. Numerical simulations show that the resonance wavelength can be modulated by changing the parameters of the air holes and thickness of gold layer. At the resonance wavelength 1550 nm, the loss is 3.8045 dB/m in x-polarization and the loss is 28464 dB/m in y-polarization by adjusting the size of the gold-coated holes and the place of air holes. Results show that the loss of y-polarized mode is much larger than the loss of x-polarized mode. The y-polarized mode is suppressed, and only x-polarized mode can be guided at the resonance wavelength of y-polarized mode. The results indicate that the mode polarized in one direction can be filtered out selectively by adjusting the diameter of air holes, and the filtering effect in a communication band is achieved.
Źródło:
Optica Applicata; 2018, 48, 4; 609-620
0078-5466
1899-7015
Pojawia się w:
Optica Applicata
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Adsorption efficiency of pentafluorobenzene on ionic liquids-based silicas
Autorzy:
Wang, X.
Lin, L.
Xie, J.
Yan, X.
Xiao, W.
Tian, P.
Powiązania:
https://bibliotekanauki.pl/articles/778559.pdf
Data publikacji:
2018
Wydawca:
Zachodniopomorski Uniwersytet Technologiczny w Szczecinie. Wydawnictwo Uczelniane ZUT w Szczecinie
Tematy:
ionic liquids based silica
pentafl uorobenzene
adsorption
solid-phase extraction
Opis:
The adsorption of pentafl uorobenzene on nine ionic liquid-based silicas was investigated using solid phase extraction. The effects of several variables such as the type of ionic liquid groups, adsorption time, temperatures and water ratio in the solution system were experimentally evaluated. The imidazole-chloride ionic liquid group based silica exhibited the highest adsorption efficiency under the optimized conditions of 5 min adsorption at 30°C in water/methanol (30:70, vol%) solution. In addition, the effects of pH, as well as type and concentrations of chloride salts were investigated. At pH values other than neutral and high salt concentration, the adsorption efficiency was reduced. Finally, the relative standard deviation of less than 5.8% over a 5-day period showed a high precision for the nine tested sorbents.
Źródło:
Polish Journal of Chemical Technology; 2018, 20, 3; 47-52
1509-8117
1899-4741
Pojawia się w:
Polish Journal of Chemical Technology
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Hydrogen sulfide modulates gastric acid secretion in rats via involvement of substance P and nuclear factor-kappaB signaling
Autorzy:
Sun, H.-Z.
Gong, X.-Y.
Wu, L.
Wang, X.-X.
Nie, Y.-N.
Shang, R.
Wang, H.
Li, Y.-C.
Sun, Q.-F.
Gao, P.-F.
Bi, J.-X.
Powiązania:
https://bibliotekanauki.pl/articles/69523.pdf
Data publikacji:
2018
Wydawca:
Polskie Towarzystwo Fizjologiczne
Tematy:
hydrogen sulphide
gastric acid secretion
rat
nuclear factor-kappaB
substance P
transient receptor potential vanilloid type 1
neurokinin-1
Źródło:
Journal of Physiology and Pharmacology; 2018, 69, 3
0867-5910
Pojawia się w:
Journal of Physiology and Pharmacology
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Simultaneous extraction of vanadium and chromium from vanadium slag using low-pressure liquid phase oxidation method
Autorzy:
Xia, J.-P.
Zheng, S.-L.
Wang, S.-N.
Liu, B.
Zou, X.
Powiązania:
https://bibliotekanauki.pl/articles/110818.pdf
Data publikacji:
2018
Wydawca:
Politechnika Wrocławska. Oficyna Wydawnicza Politechniki Wrocławskiej
Tematy:
vanadium slag
low pressure leaching
liquid phase oxidation
kinetics
Opis:
A low-pressure liquid oxidation method was proposed and proven to be effective to extract vanadium and chromium simultaneously from the vanadium slag in concentrated NaOH aqueous solutions. The effect of temperature, NaOH mass concentration, liquid-to-solid mass ratio, stirring speed and pressure on the extraction of vanadium and chromium in NaOH aqueous solutions were systematically investigated. Under the optimal reaction conditions (temperature of 473 K, liquid-to-solid mass ratio of 6:1, stirring speed of 700 rpm, NaOH mass concentration of 50%, pressure of 1 MPa and reaction time of 180 min), the vanadium and chromium recovery reached 95% and 90%, respectively. It was found that the reaction temperature and NaOH concentration were important factors for the extraction of vanadium and chromium. The kinetics of the decomposition of vanadium slag in concentrated NaOH aqueous under low pressure was analyzed using the shrinking core model, and the results indicated that the extraction of vanadium and chromium were both governed by the internal diffusion step, with apparent activation energies calculated to be 26.22 and 32.79 kJ/mol, respectively.
Źródło:
Physicochemical Problems of Mineral Processing; 2018, 54, 2; 609-619
1643-1049
2084-4735
Pojawia się w:
Physicochemical Problems of Mineral Processing
Dostawca treści:
Biblioteka Nauki
Artykuł
Wyświetlanie 1-15 z 35

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