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Wyświetlanie 1-15 z 382
Tytuł:
cDNA cloning, gene organization and expression analysis of human peptidylarginine deiminase type VI.
Autorzy:
Zhang, Jiayi
Dai, Jianliang
Zhao, Enpeng
Lin, Yun
Zeng, Li
Chen, Jinzhong
Zheng, Huari
Wang, Yu
Li, Xin
Ying, Kang
Xie, Yi
Mao, YuMin
Powiązania:
https://bibliotekanauki.pl/articles/1041522.pdf
Data publikacji:
2004
Wydawca:
Polskie Towarzystwo Biochemiczne
Tematy:
ovary
ePAD
peptidylarginine deiminase
hPADVI
Opis:
Peptidylarginine deiminase (PAD) catalyzes the post-translational modification of protein through the conversion of arginine to citrulline in the presence of calcium ions. Human, similar to rodents, has four isoforms of PAD (type I, II, III and IV/V), each of which is distinct in substrate specificity and tissue specific expression. In our large-scale sequencing project, we identified a new human PAD cDNA from a human fetal brain cDNA library. The putative protein encoded by this cDNA is designated hPADVI. Expression analysis of hPADVI showed that it is mainly expressed in adult human ovary and peripheral blood leukocytes. We conclude that hPADVI may be orthologous to mouse ePAD, basing on sequence comparison, chromosome localization and exon-intron structure analysis. PAD-mediated deimination of epithelial cell keratin resulting in cytoskeletal remodeling suggests a possible role for hPADVI in cytoskeletal reorganization in the egg and in early embryo development. This study describes a new important member of the human PAD family.
Źródło:
Acta Biochimica Polonica; 2004, 51, 4; 1051-1058
0001-527X
Pojawia się w:
Acta Biochimica Polonica
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Gradient flow optimization for reducing blocking effects of transform coding
Autorzy:
Gao, F.
Li, X.
Wang, X.
Wee, W. G.
Powiązania:
https://bibliotekanauki.pl/articles/907251.pdf
Data publikacji:
2004
Wydawca:
Uniwersytet Zielonogórski. Oficyna Wydawnicza
Tematy:
przepływ gradientu
efekt blokowania
kodowanie transformatowe
gradient flow
blocking effects
optimization
transform coding
Opis:
This paper addresses the problem of reducing blocking effects in transform coding. A novel optimization approach using the gradient flow is proposed. Using some properties of the gradient flow on a manifold, an optimized filter design method for reducing the blocking effects is presented. Based on this method, an image reconstruction algorithm is derived. The algorithm maintains the fidelity of images while reducing the blocking effects. Experimental tests demonstrate that the presented algorithm is effective.
Źródło:
International Journal of Applied Mathematics and Computer Science; 2004, 14, 1; 105-111
1641-876X
2083-8492
Pojawia się w:
International Journal of Applied Mathematics and Computer Science
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Ribosomal proteins L34 and S29 of amphioxus Branchiostoma belcheri tsingtauense: cDNAs cloning and gene copy number
Autorzy:
Liu, Lina
Zhang, Shicui
Liu, Zhenhui
Li, Hongyan
Liu, Mei
Wang, Yongjun
Ma, Lifang
Powiązania:
https://bibliotekanauki.pl/articles/1041331.pdf
Data publikacji:
2005
Wydawca:
Polskie Towarzystwo Biochemiczne
Tematy:
amphioxus
ribosomal protein
S29
copy number
Branchiostoma
L34
Opis:
The complete cDNA and deduced amino-acid sequences of ribosomal proteins L34 (AmphiL34) and S29 (AmphiS29) from the amphioxus Branchiostoma belcheri tsingtauense were identified in this study. The AmphiL34 cDNA is 435 nucleotides in length and encodes a 118 amino-acid protein with calculated molecular mass of 13.6 kDa. It shares 53.6-67.5% amino-acid sequence identity with its eukaryotic counterparts including human, mouse, rat, pig, frog, catfish, fruit fly, mosquito, armyworm, nematode and yeast. The AmphiS29 cDNA comprises 453 nucleotides and codes for a 56 amino-acid protein with a calculated molecular mass of 6.6 kDa. It shows 66.1-78.6% amino-acid sequence identity to eukaryotic S29 proteins from human, mouse, rat, pig, zebrafish, seahorse, fruit fly, nematode, sea hare and yeast. AmphiL34 contains a putative nucleolar localization signal, while AmphiS29 has a zinc finger-like domain. A phylogenetic tree deduced from the conserved sequences of AmphiL34 and AmphiS29 and other known counterparts indicates that the positions of AmphiL34/AmphiS29 are intermediate between the vertebrate and invertebrate L34/S29. Southern blot analysis demonstrates the presence of one copy of the L34 gene and 2-3 copies of the S29 gene in the genome of the amphioxus B. belcheri tsingtauense. This is in sharp contrast to the existence of 7-9 copies of the L34 gene and 14-17 copies of the S29 gene in the rat genome. These date suggest that housekeeping genes like AmphiL34 and AmphiS29 have undergone large-scale duplication in the chordate lineage.
Źródło:
Acta Biochimica Polonica; 2005, 52, 4; 857-862
0001-527X
Pojawia się w:
Acta Biochimica Polonica
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
A novel polypeptide from Cervus nippon Temminck proliferation of epidermal cells and NIH3T3 cell line
Autorzy:
Guan, Shu-Wen
Duan, Leng-Xin
Li, Yuan-Yuan
Wang, Ben-Xiang
Zhou, Qiu-Li
Powiązania:
https://bibliotekanauki.pl/articles/1041255.pdf
Data publikacji:
2006
Wydawca:
Polskie Towarzystwo Biochemiczne
Tematy:
polypeptide
velvet antler
promoting cell proliferation
Opis:
A novel polypeptide, velvet antler polypeptide (VAPPs), having a stimulary effect on proliferation of some cell was isolated from the velvet antler of sika deer (Cervus nippon Temminck). This polypeptide consists of a single chain of 32 amino-acid residues VLSAT DKTNV LAAWG KVGGN APAFG AEALE RM. VAPPs showed marked stimulary effect on rat epidermal cells and NIH3T3 cell line (dose range from 10-40 mg·L-1 and 5-80 mg·L-1, respectively).
Źródło:
Acta Biochimica Polonica; 2006, 53, 2; 395-397
0001-527X
Pojawia się w:
Acta Biochimica Polonica
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Growth of β-$Ga_2O_3$ Nanorods and Photoluminescence Properties
Autorzy:
Zhang,, S.
Zhuang, H.
Xue, C.
Li, B.
Shen, J.
Wang, D.
Powiązania:
https://bibliotekanauki.pl/articles/1814023.pdf
Data publikacji:
2007-12
Wydawca:
Polska Akademia Nauk. Instytut Fizyki PAN
Tematy:
68.65.-k
79.60.Jv
81.15.Cd
Opis:
β-$Ga_2O_3$ nanorods were successfully fabricated through annealing $Ga_2O_3$/Mo films deposited on the Si (111) substrate by radio frequency magnetron sputtering technique. The morphology and structure of the as-synthesized nanorods were characterized by X-ray diffraction, scanning electron microscopy, high-resolution transmission electron microscopy, and energy dispersive X-rays spectroscopy. The results show that the formed nanorods are single-crystalline $Ga_2O_3$ with monoclinic structure. The diameters of nanorods are 200 nm and lengths typically up to several micrometers. A photoluminescence spectrum at room temperature under excitation at 325 nm exhibits two strong blue-light peaks located at about 413.0 nm and 437.5 nm, attributed to the recombination of bound electron-hole exciton in β-$Ga_2O_3$ single crystal. The growth process of the β-$Ga_2O_3$ nanorods is probably dominated by conventional vapor-solid mechanism.
Źródło:
Acta Physica Polonica A; 2007, 112, 6; 1195-1201
0587-4246
1898-794X
Pojawia się w:
Acta Physica Polonica A
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Amino acid substitutions of His296 alter the catalytic properties of Zymomonas mobilis 10232 levansucrase
Autorzy:
Li, Shu
Chen, Ming
Li, Gang
Yan, Yong
Yu, Hai
Zhan, Yu
Peng, Zi
Wang, Jin
Lin, Min
Powiązania:
https://bibliotekanauki.pl/articles/1040841.pdf
Data publikacji:
2008
Wydawca:
Polskie Towarzystwo Biochemiczne
Tematy:
Zymomonas mobilis
His296
levansucrase
levan
transfructosylation activity
hydrolysis activity
Opis:
His296 of Zymomonas mobilis levansucrase (EC 2.4.1.10) is crucial for the catalysis of the transfructosylation reaction. The three-dimensional structures of levansucrases revealed the His296 is involved in the substrate recognition and binding. In this study, nine mutants were created by site-directed mutagenesis, in which His296 was substituted with amino acids of different polarity, charge and length. The substitutions of His296 with Arg or Trp retained partial hydrolysis and transfructosylation activities. The positively charged Lys substitution resulted in a 2.5-fold increase of sucrose hydrolysis. Substitutions with short (Cys or Ser), negatively charged (Glu) or polar (Tyr) amino acids virtually abolished both the activities. Analysis of transfructosylation products indicated that the mutants synthesized different oligosaccharides, suggesting that amino acid substitutions of His296 strongly affected both the enzyme activity and transfructosylation products.
Źródło:
Acta Biochimica Polonica; 2008, 55, 1; 201-206
0001-527X
Pojawia się w:
Acta Biochimica Polonica
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Easy Vortex Motion in an Artificial Channel of $YBa_2Cu_3O_{7-δ}$ Superconducting Films
Autorzy:
Jukna, A.
Barboy, I.
Jung, G.
Abrutis, A.
Li, X.
Wang, D.
Sobolewski, R.
Powiązania:
https://bibliotekanauki.pl/articles/1813381.pdf
Data publikacji:
2008-03
Wydawca:
Polska Akademia Nauk. Instytut Fizyki PAN
Tematy:
74.25.Sv
74.25.Qt
74.78.Bz
Opis:
Quasi-Josephson effect produced by a coherent vortex motion in the horizontal part of the laser-performedΠ-shaped channel of a $YBa_2Cu_3O_{7-δ}$ superconducting bridge was investigated by means of electric transport measurements. We observed that in our structures, in a limited range of temperatures and bias currents, the vortices were confined in the channel only and moved coherently with the velocity of $3×10^4$ m/s. The corresponding current-voltage characteristics of the bridge exhibited Josephson-like voltage steps with the amplitude dependent on temperature, but independent of the bias current.
Źródło:
Acta Physica Polonica A; 2008, 113, 3; 959-962
0587-4246
1898-794X
Pojawia się w:
Acta Physica Polonica A
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Fabrication of High-Density GaN Nanowires through Ammoniating $Ga_2O_3//Nb$ Films
Autorzy:
Zhuang, H.
Li, B.
Zhang, S.
Zhang, X.
Xue, Ch.
Wang, D.
Shen, J.
Powiązania:
https://bibliotekanauki.pl/articles/1813498.pdf
Data publikacji:
2008-02
Wydawca:
Polska Akademia Nauk. Instytut Fizyki PAN
Tematy:
68.65.-k
81.05.Ea
81.15.Cd
Opis:
High-density GaN nanowires were successfully synthesized on Si(111) substrates through ammoniating $Ga_2O_3//Nb$ films under flowing ammonia atmosphere at 950°C. The as-synthesized GaN nanowires are characterized by X-ray diffraction, selected-area-electron diffraction, Fourier transform infrared, scanning electron microscopy, and field-emission transmission electron microscopy. The results show that the synthesized nanowires are single-crystal hexagonal wurtzite GaN with diameters ranging from 30 to 100 nm and lengths up to several microns. The photoluminescence spectra measured at room temperature only exhibit a strong and broad emission peak at 367.8 nm. Finally, the growth mechanism of GaN nanowires is discussed.
Źródło:
Acta Physica Polonica A; 2008, 113, 2; 723-730
0587-4246
1898-794X
Pojawia się w:
Acta Physica Polonica A
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Experimental Studies on Charging Operation of a Compact Repetitive Tesla Transformer
Autorzy:
Zhang, Z.
Zhang, J.
Yang, H.
Qian, B.
Meng, Z.
Li, D.
Wang, S.
Cao, Y.
Zhou, W.
Powiązania:
https://bibliotekanauki.pl/articles/1807860.pdf
Data publikacji:
2009-06
Wydawca:
Polska Akademia Nauk. Instytut Fizyki PAN
Tematy:
84.30.Ng
84.30.Jc
84.30.Qi
84.70.+p
Opis:
Charging operations of a compact Tesla transformer were experimentally investigated, in single-shot and rep-rate (50 pps for 1 s) modes, respectively. The charging limitations were also explored. The experimental results were compared and analyzed. The maximum secondary charging voltages of the Tesla transformer were measured to be 380 kV and 300 kV in single-shot and rep-rate modes, respectively. The RMS pulse-to-pulse instability of the secondary charging voltage is generally less than 10% but increases with the increasing initial voltage across the primary capacitor. Since the secondary capacitor of the Tesla transformer is a pulse forming line (PFL), continued operation is possible if there is breakdown in the PFL. Furthermore, operation can even be continued under occasional breakdown for some pulses, without the effects on the operations of subsequent pulses.
Źródło:
Acta Physica Polonica A; 2009, 115, 6; 973-975
0587-4246
1898-794X
Pojawia się w:
Acta Physica Polonica A
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Improving Triggering Characteristics for a Surface-Flashover Triggered Vacuum Switch
Autorzy:
He, Z.
Wang, L.
Li, F.
Yao, W.
Dong, M.
Zhao, C.
Powiązania:
https://bibliotekanauki.pl/articles/1807889.pdf
Data publikacji:
2009-06
Wydawca:
Polska Akademia Nauk. Instytut Fizyki PAN
Tematy:
52.80.Vp
52.75.Kq
52.25.Mq
Opis:
A surface-breakdown triggered vacuum switch with parallel disk electrodes and semiconductor surface flashover trigger electrode has many advantages, it is easy to be triggered and machined. The resistance of the trigger gap has been increased in terms of modifying the thickness of the semiconductor layer and improving coating process technology. The trigger gap resistance, trigger voltage and trigger waveforms were experimentally tested. The results show that the triggering characteristics can be improved by increasing the trigger resistance.
Źródło:
Acta Physica Polonica A; 2009, 115, 6; 1019-1021
0587-4246
1898-794X
Pojawia się w:
Acta Physica Polonica A
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Theoretical Analysis of the TE Mode Cerenkov Type Second Harmonic Generation in Ion-Implanted X-Cut Lithium Niobate Planar Waveguides
Autorzy:
Du, G.
Li, G.
Zhao, S.
Qiao, W.
Yang, K.
An, J.
Li, M.
Wang, J.
Wang, W.
Powiązania:
https://bibliotekanauki.pl/articles/1808109.pdf
Data publikacji:
2009-03
Wydawca:
Polska Akademia Nauk. Instytut Fizyki PAN
Tematy:
61.72.Dd
42.82.Et
Opis:
We present a study of the Cerenkov configuration second harmonic generation in X-cut ion-implanted lithium niobate waveguides. An approximate solution of conversion efficiency is given and plotted which shows that it is very sensitive to the waveguide depth and pump wavelength. The results can be used in the design of waveguides for the efficient second harmonic generation in the Cerenkov configuration.
Źródło:
Acta Physica Polonica A; 2009, 115, 3; 685-689
0587-4246
1898-794X
Pojawia się w:
Acta Physica Polonica A
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Cloning and characterization of a pathogenesis-related gene (ThPR10) from Tamarix hispida
Autorzy:
Zhang, R.
Wang, Y.
Liu, G.
Li, H.
Powiązania:
https://bibliotekanauki.pl/articles/19850.pdf
Data publikacji:
2010
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Opis:
A PR10 gene (ThPR10) was cloned from Tamarix hispida and characterized. Real-time RT-PCR was employed to evaluate gene expression levels. ThPR10 was expressed in both leaves and roots of T. hispida under normal growth conditions, and can be highly induced in both leaf and root tissues by abiotic stresses including NaCl, PEG, cold, CdCl2, and ABA (abscisic acid) treatments. Our results indicated that ThPR10 is involved in the abiotic stress response, and regulated by an ABA-dependent signaling pathway. Subsequently, ThPR10 was localized at the subcellular level. The gene was fused with the GFP N-terminal driven by CaMV35S promoter and transiently expressed in onion epidermal cells. This strategy localized the ThPR10 protein to the nucleus of onion epidermal cells, suggesting that the pathogenesis-related proteins play a functional role in the cell nucleus.
Źródło:
Acta Biologica Cracoviensia. Series Botanica; 2010, 52, 2
0001-5296
Pojawia się w:
Acta Biologica Cracoviensia. Series Botanica
Dostawca treści:
Biblioteka Nauki
Artykuł
Wyświetlanie 1-15 z 382

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