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Wyświetlanie 1-15 z 44
Tytuł:
Indirect enzyme-linked immunosorbent assay method based on Streptococcus agalactiae rSip-Pgk-FbsA fusion protein for detection of bovine mastitis
Autorzy:
Bu, R.-E.
Wang, I.-L.
Wu, J.-H.
Xilin, G.W.
Chen, J.-L.
Wang, H.
Powiązania:
https://bibliotekanauki.pl/articles/30156.pdf
Data publikacji:
2017
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Opis:
Objective: The aim of this study was to establish a rapid and accurate method for the detection of the Streptococcus agalactiae antibody (SA-Ab) to determine the presence of the bovine mastitis (BM)-causative pathogen. Methods: The multi-subunit fusion protein rSip-Pgk-FbsA was prokaryotically expressed and purified. The triple activities of the membrane surface-associated proteins Sip, phosphoglycerate kinase (Pgk), and fibronectin (FbsA) were used as the diagnostic antigens to establish an indirect enzyme-linked immunosorbent assay (ELISA) method for the detection of SA-Ab in BM. Results: The optimal antigen coating concentration was 2 μg/mL, the optimal serum dilution was 1:160, and the optimal dilution of the enzyme-labeled secondary antibody was 1:6000. The sensitivity, specificity, and repeatability tests showed that the method established in this study had no cross-reaction with antibodies to Streptococcus pyogenes, Escherichia coli, Staphylococcus aureus, and Staphylococcus epidermidis in the sera. The results of the sensitivity test showed that a positive result could be obtained even if the serum dilution reached 1:12,800, indicating the high sensitivity and good repeatability of the method. The positive coincidence rate of this method was 98.6%, which is higher than that of previous tests established with the Sip or Pgk mono-antigen fusion protein, respectively, demonstrating the relatively higher sensitivity of this newly established method. The detection rate for 389 clinical samples was 46.53%. Conclusions: The indirect ELISA method established in this study could provide a more accurate and reliable serological method for the rapid detection of S. agalactiae in cases of BM.
Źródło:
Polish Journal of Veterinary Sciences; 2017, 20, 2
1505-1773
Pojawia się w:
Polish Journal of Veterinary Sciences
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
A super depth of field height measurement based on local disparity
Autorzy:
Cui, L.
Liu, Y.
Yu, M.
Jiang, G.
Fan, S.
Wang, Y.
Powiązania:
https://bibliotekanauki.pl/articles/173522.pdf
Data publikacji:
2015
Wydawca:
Politechnika Wrocławska. Oficyna Wydawnicza Politechniki Wrocławskiej
Tematy:
microscopes
binocular vision
local disparity map
depth of field (DOF)
height measurement
Opis:
A super depth of field height measurement method is proposed to measure the object height with the optical stereoscopic microscope. The quasi-Euclidean epipolar rectification algorithm is utilized on the original stereoimage to obtain rectified stereoimages and calibrate two camera parameters. Then, feature points are obtained by the SURF (speed up robust feature) algorithm and their corresponding disparities are calculated. The disparity-depth of field curve is fitted by combining the step height values of a stepper motor. Moreover, through local disparity value got from feature points on the object, the relative shift height is calculated through regression analysis. Finally, according to binocular vision geometry, the thickness of the object can be calculated. Experimental results show that the measurement error in Z direction is from 1.51% to 7.71%, which indicates that the proposed method is able to measure the height of a microobject beyond depth of field within a tolerant error.
Źródło:
Optica Applicata; 2015, 45, 2; 205-214
0078-5466
1899-7015
Pojawia się w:
Optica Applicata
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Study on the design of container highway and railway automatic transfer vehicle in ocean port
Autorzy:
Dong, L.
Liu, G.
Ye, X.
Wang, W.
Powiązania:
https://bibliotekanauki.pl/articles/962741.pdf
Data publikacji:
2018
Wydawca:
Politechnika Gdańska. Wydział Inżynierii Mechanicznej i Okrętownictwa
Tematy:
ocean port
multimodal transport
highway and railway
unmanned automatic
seamless connection
Opis:
To realize “seamless” connection of ocean port container multimodal transport, efficiently carry out “door-to-door” transport of ocean port containers and overcome the shortcomings of existing highway and railway vehicles, this study takes the standard for heavy-duty container vehicles in TB1335-1996 Railway Vehicle Strength Design and Test Identification Code as the design basis and designs a new ocean port container transport vehicle in combination with automatic guidance technology. This study innovatively designs the automatic lifting system of the bogie and the docking part of the vehicle, introduces the automatic guidance technology and the remote-control technology to optimize the car body structure, and uses the SAP software to carry out the finite element analysis of the car body load capacity and Flexsim software to carry out the simulation analysis on the operation of vehicles. The designed transfer vehicle can improve the transfer efficiency of ocean port containers, reduce the transit time of field and station equipment and container transport links, and improve the level of multimodal transport and comprehensive economic benefits.
Źródło:
Polish Maritime Research; 2018, S 3; 5-12
1233-2585
Pojawia się w:
Polish Maritime Research
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Electric Interaction between Two Identical Conducting Spheres in a Uniform Electric Field
Autorzy:
Gao, X.
Wang, Q.
Li, C.
Hu, L.
Sun, G.
Powiązania:
https://bibliotekanauki.pl/articles/1401870.pdf
Data publikacji:
2015-09
Wydawca:
Polska Akademia Nauk. Instytut Fizyki PAN
Tematy:
41.20.Cv
47.65.Gx
Opis:
An experimental apparatus is devised to measure the interaction between two spheres separated by a small gap in a uniform electric field. The results show that the interaction between two conducting spheres is near that between two dielectric spheres with high permittivity. Accordingly, the calculation can be simplified by mirror image method, for it is only available for conducting system. A method using multiple mirror images of point charges is put forward to analyze the induction of two identical conducting spheres in a uniform electric field. The key operation on how to add compensative charges is emphasized and given out in detail. The results from experiment and calculation are compared, and they agree with each other very well.
Źródło:
Acta Physica Polonica A; 2015, 128, 3; 289-293
0587-4246
1898-794X
Pojawia się w:
Acta Physica Polonica A
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Technical application of petroleum logging instruments in marine logging
Autorzy:
Gu, G.
Zhao, P.
Wang, L.
Guo, H.
Powiązania:
https://bibliotekanauki.pl/articles/260000.pdf
Data publikacji:
2018
Wydawca:
Politechnika Gdańska. Wydział Inżynierii Mechanicznej i Okrętownictwa
Tematy:
oil logging instrument
pulsed neutron oxygen activation
injection profile logging
application
Opis:
To solve the problem of offshore oilfield development, based on the newly introduced pulsed neutron oxygen activation logging instrument, the application research of test design and interpretation method was carried out and applied to actual production. The structure, technical indicators and logging principles of pulsed neutron oxygen activation logging tools were introduced. The test design under different well conditions was studied, including general positive and negative injection, oil sleeve injection, single oil pipe configuration and multitubing configuration. A large amount of field test data was collected and analysed technically. A set of effective interpretation models was proposed. The corresponding interpretation software was developed. A set of test design methods and operating specifications for different well conditions were developed. Based on the conventional interpretation method, the peak selection, the double-tuber peak identification and the carbon dioxide flooding interpretation method were added. The results show that the test design and interpretation methods were applied well through a large number of field tests and production applications. Therefore, pulsed neutron oxygen activated injection profile logging technology is successfully applied in offshore oil fields.
Źródło:
Polish Maritime Research; 2018, S 3; 22-28
1233-2585
Pojawia się w:
Polish Maritime Research
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Factors regulating the compositions and distributions of dissolved organic matter in the estuaries of Jiaozhou Bay in North China
Autorzy:
Hu, J.
Zou, L.
Wang, J.
Ren, Q.
Xia, B.
Yu, G.
Powiązania:
https://bibliotekanauki.pl/articles/48070.pdf
Data publikacji:
2020
Wydawca:
Polska Akademia Nauk. Instytut Oceanologii PAN
Tematy:
organic matter
chromophoric dissolved organic matter
dissolved organic matter
particulate organic matter
total carbohydrate
amino acid
biogeochemical process
coastal environment
chlorophyll a
North China
Źródło:
Oceanologia; 2020, 62, 1
0078-3234
Pojawia się w:
Oceanologia
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
A multiplex PCR for simultaneous detection of classical swine fever virus, African swine fever virus, highly pathogenic porcine reproductive and respiratory syndrome virus, porcine reproductive and respiratory syndrome virus and pseudorabies in swines
Autorzy:
Hu, L.
Lin, X.Y.
Yang, Z.X.
Yao, X.P.
Li, G.L.
Peng, S.Z.
Wang, Y.
Powiązania:
https://bibliotekanauki.pl/articles/30249.pdf
Data publikacji:
2015
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Opis:
In this assay, we developed and evaluated a multiplex PCR (mPCR) for its ability in detecting multiple infections of swine simultaneously. Four pairs of primers were used to detect five viruses. Specific primers were designed for classical swine fever virus (CSFV), African swine fever virus (ASFV) and pseudorabies (PRV). A pair of primers was designed prudently for two different types of porcine reproductive and respiratory syndrome virus that respectively were porcine reproductive and respiratory syndrome virus (PRRSV), highly pathogenic porcine reproductive and respiratory syndrome virus (HP-PRRSV). The detection limits of the mPCR were 1.09×10⁴, 1.50×10³, 2.10×10³, 1.30×10³ and 8.97×10² copies/reaction for CSFV, ASFV, HP-PRRSV, PRRSV and PRV, respectively. A total of 49 clinical specimens were tested by the mPCR, and the result showed that co-infection by two or three viruses was 51%. In conclusion, the PCR is a useful tool for clinical diagnosis of not only single infections but also mixed infections in swines.
Źródło:
Polish Journal of Veterinary Sciences; 2015, 18, 4
1505-1773
Pojawia się w:
Polish Journal of Veterinary Sciences
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Rapid screening of monoclonal antibodies against porcine circovirus type 2 using colloidal gold-based paper test
Autorzy:
Jin, Q.Y.
Feng, L.L.
Wang, Y.B.
Li, P.
Yang, J.F.
Teng, M.
Chai, S.J.
Xing, G.X.
Zhang, G.P.
Powiązania:
https://bibliotekanauki.pl/articles/16539073.pdf
Data publikacji:
2022
Wydawca:
Polska Akademia Nauk. Czasopisma i Monografie PAN
Tematy:
porcine circovirus type 2 (PCV2)
screening of MAbs
paper test
Opis:
A proof of concept for using paper test as a suitable method in the production of monoclonal antibodies (MAbs) is reported. The paper test which detects antibodies against porcine circovirus type 2 (PCV2) using colloidal gold-labelled capsid protein as the antigen probe was applied exclusively in the screening of anti-PCV2 MAbs. It allowed the detection of 118 single cell clones within 30 min using naked eyes. MAbs with specific binding to authentic epitopes on the virus were selected using a blocking strategy in which the antibody was pre-incubated with PCV2 viral sample before applying to the test paper. Five hybridomas secreting MAbs against the capsid protein were obtained, with only three of them capable of binding to PCV2. The results were validated and confirmed using enzyme-linked immunosorbent assay and immunofluorescence assay. The paper test is simple, rapid, and independent on professional technicians and proves to be an excellent approach for the screening of MAbs against specific targets.
Źródło:
Polish Journal of Veterinary Sciences; 2022, 25, 1; 27-34
1505-1773
Pojawia się w:
Polish Journal of Veterinary Sciences
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
The Machine Learning Method of PIDVCA
Autorzy:
Li, L.
Wang, X.
Chen, G.
Powiązania:
https://bibliotekanauki.pl/articles/1841618.pdf
Data publikacji:
2020
Wydawca:
Uniwersytet Morski w Gdyni. Wydział Nawigacyjny
Tematy:
learning methods
personified intelligence
collision avoidance
dynamic collision avoidance
static collision avoidance
Opis:
Building a dynamic collision knowledge base of self-learning is one of the core contents of implementing "personified intelligence" in Personifying Intelligent Decision-making for Vessel Collision Avoidance (short for PIDVCA). In the paper, the machine learning method of PIDVCA combined with offline artificial learning and online machine learning is proposed. The static collision avoidance knowledge is acquired through offline artificial learning, and the isomeric knowledge representation integration method with process knowledge as the carrier is established, and the Dynamic collision avoidance knowledge is acquired through online machine learning guided by inference engine. A large number of simulation results show that the dynamic collision avoidance knowledge base constructed by machine learning can achieve the effect of anthropomorphic intelligent collision avoidance. It is verified by examples that the machine learning method of PIDVCA can realize target perception, target cognition and finally obtain an effective collision avoidance decision-making.
Źródło:
TransNav : International Journal on Marine Navigation and Safety of Sea Transportation; 2020, 14, 3; 533-540
2083-6473
2083-6481
Pojawia się w:
TransNav : International Journal on Marine Navigation and Safety of Sea Transportation
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Study on self-repairing and non-diffraction of Airy beams in slant atmospheric turbulence
Autorzy:
Li, Y.-Q.
Wang, L.-G.
Powiązania:
https://bibliotekanauki.pl/articles/174867.pdf
Data publikacji:
2018
Wydawca:
Politechnika Wrocławska. Oficyna Wydawnicza Politechniki Wrocławskiej
Tematy:
atmospheric turbulence
Airy beam
self-repairing
non-diffraction
numerical simulation
Opis:
The Airy beams propagation in atmospheric turbulence along a slant path was simulated numerically, based on the split-step Fourier method. Also, the self-repairing and non-diffraction characteristics of Airy beams were investigated and compared with beams propagation on a horizontal path. The effects of parameters including zenith angle, propagation distance, radii of Gaussian aperture and turbulence intensity on the two characteristics of beams were revealed. Additionally, the two characteristics of the Airy beam were compared with those of a Bessel–Gauss beam. The results showed that the two beams obscured by Gaussian apertures can be repaired after propagating some distance along a slant path. However, the non-diffraction characteristic of an Airy beam was stronger than that of a Bessel–Gauss beam and the amplitude attenuation rate of the Bessel–Gauss beam was greater than that of the Airy beam in the process of self-repairing. Results obtained can provide a theoretical basis for an outdoor experiment as well as theoretical guidance for various practical applications including laser communications, laser warning systems, and remote sensing.
Źródło:
Optica Applicata; 2018, 48, 3; 435-447
0078-5466
1899-7015
Pojawia się w:
Optica Applicata
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Demonstration of a new indicator for studying upwelling in the Northern South China Sea
Autorzy:
Lin, L.
Wang, Y.-S.
Sun, C.-C.
Li, N.
Wang, H.
Mitchell, B.G.
Wu, M.-L.
Song, H.
Wu, J.-F.
Powiązania:
https://bibliotekanauki.pl/articles/48889.pdf
Data publikacji:
2011
Wydawca:
Polska Akademia Nauk. Instytut Oceanologii PAN
Tematy:
China Sea
cluster analysis
multivariate statistical analysis
principal component analysis
remote sensing
satellite monitoring
sea surface temperature
silicate
spatial distribution
upwelling
Opis:
In order to demonstrate that silicate (SiO3-Si) can be used as an indicator to study upwelling in the northern South China Sea, hierarchical cluster analysis (CA) and principle component analysis (PCA) were applied to analyse the metrics of the data consisting of 14 physical-chemical-biological parameters at 32 stations. CA categorized the 32 stations into two groups (low and high nutrient groups). PCA was applied to identify five Principal Components (PCs) explaining 78.65% of the total variance of the original data. PCA found important factors that can describe nutrient sources in estuarine, upwelling, and non-upwelling areas. PC4, representing the upwelling source, is strongly correlated to SiO3-Si. The spatial distribution of silicate from the surface to 200 m depth clearly showed the upwelling regions, which is also supported by satellite observations of sea surface temperature.
Źródło:
Oceanologia; 2011, 53, 2
0078-3234
Pojawia się w:
Oceanologia
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Mitigation scratch on fused silica optics using CO2 laser
Autorzy:
Liu, C.-M.
Yan, Z.-H.
Yang, L.
Jiang, Y.
Zu, X.-T.
Wang, H.-J.
Liao, W.
Yuan, X.-D.
Zheng, W.-G.
Powiązania:
https://bibliotekanauki.pl/articles/174978.pdf
Data publikacji:
2016
Wydawca:
Politechnika Wrocławska. Oficyna Wydawnicza Politechniki Wrocławskiej
Tematy:
mitigation
scratch
fused silica
CO2 laser
Opis:
The scratch on a fused silica surface was treated as a chain of connected damage sites and mitigated one after another using CO2 laser irradiation. The optical microscopy image shows that a scratch with the width of about 30 μm and length of several millimeters can be completely mitigated without the formation of debris and bubbles. The mitigated scratch can survive under raster scan laser irradiation with the fluency increased up to 11.0J/cm2 at 3ns and 351nm. On the contrary, the substrate without CO2 laser mitigation is seriously damaged under this irradiation. The light modulation induced by mitigation is much smaller when the scratch is mitigated before being damaged. The light modulation is about 2 when the distance to the mitigated sample is larger than 20cm. The birefringence induced by residual stress in the mitigated scratch is measured. The retardance of the mitigated scratch before being damaged is not visible. Therefore, residual stress in this mitigated scratch before being damaged should be not a critical potential risk in laser damage.
Źródło:
Optica Applicata; 2016, 46, 3; 387-397
0078-5466
1899-7015
Pojawia się w:
Optica Applicata
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Factors Affecting the Measurement of the Percentage of Gaseous Products from Boron-based Fuel-rich Propellants
Autorzy:
Liu, L.-L.
He, G.-Q.
Wang, Y.-H.
Liu, P.-J.
Powiązania:
https://bibliotekanauki.pl/articles/358720.pdf
Data publikacji:
2014
Wydawca:
Sieć Badawcza Łukasiewicz - Instytut Przemysłu Organicznego
Tematy:
fuel-rich propellant
percentage gaseous products
combustion
filter media
Opis:
Acid-washed asbestos, carbon fibre, and MgO with carbon fibre were used as the filter media in order to compare their filtering qualities in the estimation of the percentage of gaseous products (PGP) arising from the combustion of singlebase propellants, double-base propellants, and boron-based fuel-rich propellants. The comparison was based on an analysis of the experimentally registered influence of the propellant formulation, the propellant load, the maximum chamber pressure and the thickness of the MgO filter layer on the PGP from the fuel-rich propellant, and in particular on the PGP produced by combustion of boron-based fuel-rich propellant. The results showed that the experimental values of the PGP were closer to the theoretically predicted values when carbon fibre mixed with MgO powder was used as the filter medium. The PGP of boron-based fuel-rich propellant increased when the AP was in part replaced by HMX, when the AP content was increased and when boron was in part replaced by magnesium-aluminum alloy. In terms of the apparatus used in these experiments, the propellant loading density was found to have little correspondence with the PGP for boron-based fuel-rich propellant. The optimal propellant loading density for the chamber volume of 85 cm3 was found to be 2-2.5 g, in view of the reliability and safety of the experiment. It is emphasised that the thickness of the MgO filter layer is very important for the accuracy and reliability of the experiment, and that the optimum should be determined by experiment.
Źródło:
Central European Journal of Energetic Materials; 2014, 11, 1; 15-29
1733-7178
Pojawia się w:
Central European Journal of Energetic Materials
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Ignition and Combustion Performance of the Primary Condensed-phase Combustion Products from Boron-based Fuel-rich Propellants
Autorzy:
Liu, L.-L.
He, G.-G.
Wang, Y.-H.
Hu, S.-G.
Powiązania:
https://bibliotekanauki.pl/articles/358859.pdf
Data publikacji:
2017
Wydawca:
Sieć Badawcza Łukasiewicz - Instytut Przemysłu Organicznego
Tematy:
boron-based fuel-rich propellant
primary combustion products
ignition
combustion performance
Opis:
The primary condensed-phase combustion products of a boron-based fuel-rich propellant were ignited using a high-power CO2 laser in a pressurized and windowed combustion chamber under variable pressure. The ignition and combustion performances were characterized using an ultraviolet-visible spectrometer and a high-speed camera. The experimental results showed that the combustion of the condensed-phase combustion products originate from the combustion of carbon, and boron may not take any part in the combustion process because of the formation of a thick boron oxide coating and agglomeration after the primary combustion process. Both particle size and the ingredients play an important role in the combustion reaction of the condensed-phase products. It was observed that a lower particle size and a higher boron carbide content are beneficial for the combustion reaction of the products; higher primary and secondary combustion pressures clearly improved the secondary combustion efficiency of the propellant in the combustion reaction.
Źródło:
Central European Journal of Energetic Materials; 2017, 14, 2; 448-460
1733-7178
Pojawia się w:
Central European Journal of Energetic Materials
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Disparity servoing based fast autofocusing method for stereomicroscope
Autorzy:
Liu, Y.
Yu, M.
Cui, L.
Jiang, G.
Wang, G.
Fan, S.
Powiązania:
https://bibliotekanauki.pl/articles/174449.pdf
Data publikacji:
2016
Wydawca:
Politechnika Wrocławska. Oficyna Wydawnicza Politechniki Wrocławskiej
Tematy:
autofocusing
stereomicroscope
stereomicroscopic images
disparity
Opis:
A disparity servoing based fast autofocusing method is proposed for stereomicroscopes according to linear relationship between the disparity change in stereomicroscopic images and the move distance of a motorized translation stage. For a certain stereomicroscope, the calibration of a disparity range of clear images at each magnification is implemented offline. After that, the disparity of the stereomicroscopic image is used as an index to represent the sharpness of an arbitrary image. If the disparity does not satisfy the requirement, move steps and direction of a step motor are calculated by utilizing the linear relationship between the disparity change and the move distance of the stage. The iteration will be continued until the disparity of the captured stereomicroscopic image approximates to the clearest disparity. The experimental results show that the proposed method only requires a few iterations and less time to reach the focus position, and the disparity error is less than 0.5 pixel.
Źródło:
Optica Applicata; 2016, 46, 4; 651-663
0078-5466
1899-7015
Pojawia się w:
Optica Applicata
Dostawca treści:
Biblioteka Nauki
Artykuł
Wyświetlanie 1-15 z 44

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