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Wyświetlanie 1-15 z 138
Tytuł:
A new strategy: high level expression and immunogenicity analysis of triplicate repeated multigenes in Mycoplasma hyopneumoniae
Autorzy:
Li, J.
Wang, G.
Powiązania:
https://bibliotekanauki.pl/articles/16647587.pdf
Data publikacji:
2023
Wydawca:
Polska Akademia Nauk. Czasopisma i Monografie PAN
Tematy:
multi-epitope fusion protein
Mycoplasma hyopneumoniae
new generation vaccines
Opis:
Highly immunogenic nucleotide fragments from 3 genes of Mycoplasma hyopneumoniae strain 232 were selected using information software technology. After repeating each fragment three times, a total of 9 nucleotide fragments were joined together to form a new nucleotide sequence called Mhp2321092bp. Mhp2321092bp was directly synthesized and cloned into a pET100 vector and expressed in Escherichia coli. After purification, the proteins were successfully validated by SDS-PAGE and Western blotting using mouse His-tag antibody and pig anti-Mhp serum. BALB/c mice were intraperitoneally injected with purified proteins in the high-dose (100 µg), medium-dose group (50 µg) and low-dose (10 µg) groups. Mice in each group were injected on day 1, day 8 and day 15 of feeding, respectively. Serum samples were collected from all mice on the day before immunization and on day 22 after immunization. The antibody level in the mouse serum was detected using western blotting using purified expressed proteins as antigens. IL-2, TNF-α and IFN-γ were simultaneously detected in the mouse serum by ELISA. The results showed that the 60 kDa protein was successfully expressed and reacted specifically with the specific serum Mhp His-Tag mouse monoclonal antibody and pig anti-Mhp serum. From day 0 to day 22 of immunization, IFN-γ increased from 269.52 to 467.74 pg/mL, IL-2 increased from 14.03 to 145.16 pg/mL, and TNF-α increased from 6.86 to 12.37 pg/mL. The IgG antibody in mice increased significantly from 0 day to day 22 after immunization. This study suggests that the expressed recombinant protein may serve as one of the novel vaccine candidates for Mhp.
Źródło:
Polish Journal of Veterinary Sciences; 2023, 26, 2; 275-283
1505-1773
Pojawia się w:
Polish Journal of Veterinary Sciences
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Association between apparent temperature and hypertension hospital admissions: a case study in rural areas in western China
Autorzy:
Zhai, G.
Wang, Y.
Zhang, K.
Tian, Y.
Powiązania:
https://bibliotekanauki.pl/articles/28763043.pdf
Data publikacji:
2023
Wydawca:
Instytut Medycyny Wsi
Źródło:
Annals of Agricultural and Environmental Medicine; 2023, 30, 3; 490-497
1232-1966
Pojawia się w:
Annals of Agricultural and Environmental Medicine
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Rapid screening of monoclonal antibodies against porcine circovirus type 2 using colloidal gold-based paper test
Autorzy:
Jin, Q.Y.
Feng, L.L.
Wang, Y.B.
Li, P.
Yang, J.F.
Teng, M.
Chai, S.J.
Xing, G.X.
Zhang, G.P.
Powiązania:
https://bibliotekanauki.pl/articles/16539073.pdf
Data publikacji:
2022
Wydawca:
Polska Akademia Nauk. Czasopisma i Monografie PAN
Tematy:
porcine circovirus type 2 (PCV2)
screening of MAbs
paper test
Opis:
A proof of concept for using paper test as a suitable method in the production of monoclonal antibodies (MAbs) is reported. The paper test which detects antibodies against porcine circovirus type 2 (PCV2) using colloidal gold-labelled capsid protein as the antigen probe was applied exclusively in the screening of anti-PCV2 MAbs. It allowed the detection of 118 single cell clones within 30 min using naked eyes. MAbs with specific binding to authentic epitopes on the virus were selected using a blocking strategy in which the antibody was pre-incubated with PCV2 viral sample before applying to the test paper. Five hybridomas secreting MAbs against the capsid protein were obtained, with only three of them capable of binding to PCV2. The results were validated and confirmed using enzyme-linked immunosorbent assay and immunofluorescence assay. The paper test is simple, rapid, and independent on professional technicians and proves to be an excellent approach for the screening of MAbs against specific targets.
Źródło:
Polish Journal of Veterinary Sciences; 2022, 25, 1; 27-34
1505-1773
Pojawia się w:
Polish Journal of Veterinary Sciences
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Seroprevalence of antibodies to classical swine fever virus and porcine reproductive and respiratory syndrome virus in healthy pigs in Hunan Province, China
Autorzy:
Yu, H.
Zhang, L.
Cai, Y.
Hao, Z.
Luo, Z.
Peng, T.
Liu, L.
Wang, N.
Wang, G.
Deng, Z.
Zhan, Y.
Powiązania:
https://bibliotekanauki.pl/articles/16539345.pdf
Data publikacji:
2022
Wydawca:
Polska Akademia Nauk. Czasopisma i Monografie PAN
Tematy:
classical swine fever virus (CSFV)
porcine reproductive and respiratory syndrome virus (PRRSV)
pig
seroprevalence
antibody
Opis:
Classical swine fever (CSF) and porcine reproductive and respiratory syndrome (PRRS) are responsible for major economic losses and represent a threat to the swine industry worldwide. Routine surveillance serology for CSF and PRRS viruses is critical to maintaining the health status of sow farms in Hunan Province, which is one of the top pig production provinces in China. The aim of our study was to investigate the serological statistics of CSF virus (CSFV) and PRRS virus (PRRSV) in Hunan Province. The cohort serum samples were collected from vaccinated and unvaccinated pigs. Our findings showed that the average rates of CSFV and PRRSV antibody seropositivity were 82.2% (95% CI: 80.1-84.3) and 84.8% (95% CI: 82.5-87.1), respectively, in the immunized group and that these rates were higher than those in the unvaccinated group (58.6% for CSFV and 47.8% for PRRSV). Additionally, the level of CSFV antibody in piglet serum declined gradually with age, whereas PRRSV-specific antibody level increased initially (1 to 2 weeks old) and then declined with age (2 to 4 weeks old). In summary, we investigated the difference in CSFV/PRRSV antibody levels among piglets at various weeks old (1 to 4 weeks) to further establish the duration of maternal immunity in piglets. In addition, routine monitoring of CSFV/PRRSV antibodies in immunized pigs was carried out to evaluate the efficacy of vaccination.
Źródło:
Polish Journal of Veterinary Sciences; 2022, 25, 3; 375-381
1505-1773
Pojawia się w:
Polish Journal of Veterinary Sciences
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Antibacterial spectrum of four compounds from yeasts in koumiss
Autorzy:
Chen, Y.J.
Du, C.G.
Guo, Y.Q.
Zhao, Y.F.
Aorigele, C.
Wang, C.J.
Simujide, H.
Aqima, W.
Zhang, X.Y.
Powiązania:
https://bibliotekanauki.pl/articles/2087160.pdf
Data publikacji:
2021
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
Kluyveromyces marxianus
Saccharomyces cerevisiae
Escherichia coli
minimum inhibition concentration
minimum bactericidal concentration
Źródło:
Polish Journal of Veterinary Sciences; 2021, 24, 2; 167-173
1505-1773
Pojawia się w:
Polish Journal of Veterinary Sciences
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Expression and immunogenicity study of a novel mhp183 gene fragment of Mycoplasma hyopneumoniae
Autorzy:
Xu, M.
Zheng, J.
Hu, S.
Wang, G.
Powiązania:
https://bibliotekanauki.pl/articles/2087132.pdf
Data publikacji:
2021
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
Mycoplasma hyopneumoniae
Mhp183 gene
new generation vaccines
Źródło:
Polish Journal of Veterinary Sciences; 2021, 24, 4; 553-561
1505-1773
Pojawia się w:
Polish Journal of Veterinary Sciences
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
The Bending, Impact Fracture Behavior and Characteristics of Stainless Steel Clad Plates with Different Rolling Temperature
Autorzy:
An, Q.
Fan, K. Y.
Ge, Y. F.
Liu, B. X.
He, J.
Wang, S.
Chen, C. X.
Ji, P. G.
Tolochko, O.
Powiązania:
https://bibliotekanauki.pl/articles/2049433.pdf
Data publikacji:
2021
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
stainless steel clad plate
interface characteristics
bending behavior
impact morphologies
delamination crack
Opis:
The interface characteristics, bending and impact behavior, as well as fracture characteristics of stainless steel clad plates fabricated by vacuum hot rolling at different rolling temperatures of 1100°C, 1200°C and 1300°C are investigated in detail. The interface bonding strength is gradually increased with the increasing rolling temperature due to the sufficient diffusion behavior of alloy element. The bending toughness and impact toughness are gradually decreased, while the bending strength increase with the increase of the rolling temperature, which is attributed to mechanisms of matrix softening and interface strengthening at high rolling temperature. Due to the weak interface at 1100°C, the bending and impact crack propagation path was displaced by delamination cracks, which in turn lead to reduction in stress intensity of the main crack, playing an effective role in toughening the stainless steel clad plates. Moreover, the impact fracture morphologies of clad plates show a typical ductile-brittle transition phenomenon, which is attributed to the matrix softening behavior with the increasing rolling temperature.
Źródło:
Archives of Metallurgy and Materials; 2021, 66, 1; 229-239
1733-3490
Pojawia się w:
Archives of Metallurgy and Materials
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Antibiotic resistance and molecular characteristics of Staphylococcus aureus isolated from pigs in Hunan, China
Autorzy:
Zhang, X.
Wang, G.
Yin, C.
Powiązania:
https://bibliotekanauki.pl/articles/2087249.pdf
Data publikacji:
2020
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
antibiotic resistance
molecular characterization
pig-associated S. aureus
Hunan
Źródło:
Polish Journal of Veterinary Sciences; 2020, 23, 4; 563-570
1505-1773
Pojawia się w:
Polish Journal of Veterinary Sciences
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Characterization of serum adiponectin and leptin in healthy perinatal dairy cows or cows with ketosis, and their effectson ketosis involved indices
Autorzy:
Shen, L.
Qian, B.
Xiao, J.
Zhu, Y.
Hussain, S.
Deng, J.
Peng, G.
Zuo, Z.
Zou, L.
Yu, S.
Ma, X.
Zhong, Z.
Ren, Z.
Wang, Y.
Liu, H.
Zhou, Z.
Cai, D.
Hu, Y.
Zong, X.
Cao, S.
Powiązania:
https://bibliotekanauki.pl/articles/2087289.pdf
Data publikacji:
2020
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
adiponectin
dairy cows
ketosis
leptin
perinatal period
Źródło:
Polish Journal of Veterinary Sciences; 2020, 23, 3; 373-381
1505-1773
Pojawia się w:
Polish Journal of Veterinary Sciences
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Factors regulating the compositions and distributions of dissolved organic matter in the estuaries of Jiaozhou Bay in North China
Autorzy:
Hu, J.
Zou, L.
Wang, J.
Ren, Q.
Xia, B.
Yu, G.
Powiązania:
https://bibliotekanauki.pl/articles/48070.pdf
Data publikacji:
2020
Wydawca:
Polska Akademia Nauk. Instytut Oceanologii PAN
Tematy:
organic matter
chromophoric dissolved organic matter
dissolved organic matter
particulate organic matter
total carbohydrate
amino acid
biogeochemical process
coastal environment
chlorophyll a
North China
Źródło:
Oceanologia; 2020, 62, 1
0078-3234
Pojawia się w:
Oceanologia
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Revealing the core transcriptome modulating plant growth phase in Arabidopsis thalianaby RNA sequencingand coexpression analysis of the FHY3 FAR1 mutant
Autorzy:
Wang, W.
Zhao, G.
Wu, S.
Hua, W.
Zhang, T.
Ruan, R.
Cheng, Y.
Powiązania:
https://bibliotekanauki.pl/articles/2130692.pdf
Data publikacji:
2020
Wydawca:
Polskie Towarzystwo Botaniczne
Tematy:
weekly transcriptome analysis
plant development
different expression genes
(DEGs)
immune response
Opis:
Plants must continually calibrate their growth in response to the environment throughout their whole life cycle. Revealing the regularity of plant early growth and development is of great significance to plant genetic modification. It was previously demonstrated that loss of two key light signaling transcription factors, FHY3 and FAR1, can cause a stunted stature in the plant adult stage, and numerous defense response genes can be continuously activated. In this study, we performed a time-course transcriptome analysis of the early 4 weeks of leaf samples from wild plants and their fhy3 and far1 transcription factors. By comparative transcriptome analysis, we found that during the early 4 weeks of plant growth, plants primarily promoted morphogenesis by organizing their microtubules in the second week. In the third week, plants began to trigger large- scale defense responses to resist various external stresses. In the fourth week, increased photosynthetic efficiency promoted rapid biomass accumulation. Weighted gene coexpression network analysis of FHY3 and FAR1 revealed that the two light signaling transcription factors may be originally involved in the regulation of genes during embryonic development, and in the later growth stage, they might regulate gene expression of some defense-related genes to balance plant growth and immunity. Remarkably, our yeast two-hybrid and bimolecular fluorescence complementation experiments showed that FAR1 interacts with the immune signaling factor EDS1. Taken together, this study demonstrates the major biological processes occurring during the early 4 weeks of plant growth. The light signaling transcription factors, FHY3 and FAR1, may integrate light signals with immune signals to widely regulate plant growth by directly interacting with EDS1.
Źródło:
Acta Societatis Botanicorum Poloniae; 2020, 89, 2
0001-6977
2083-9480
Pojawia się w:
Acta Societatis Botanicorum Poloniae
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Room-temperature sensing performance of hydrogen using palladium-based film by optical setup
Autorzy:
Yao, Yiyun
Corso, Alain J.
Bazzan, Marco
Tessarolo, Enrico
Wang, Zhanshan
Huang, Qiushi
Qi, Runze
Martucci, A.
Pelizzo, Maria G.
Powiązania:
https://bibliotekanauki.pl/articles/1835811.pdf
Data publikacji:
2020
Wydawca:
Politechnika Wrocławska. Oficyna Wydawnicza Politechniki Wrocławskiej
Tematy:
hydrogen detection
palladium-based film
palladium–platinum alloy film
optical sensor
Opis:
H2 sensing performance of novel Pd–Pt alloy films has been compared with those obtained by using Pd films and H2-reducted PdO films. Two different detecting systems were used to measure the hydrogenation and de-hydrogenation phases with a H2 concentration of both 5% v/v nitrogen and 1% v/v nitrogen at room temperature. The sensitivity loss observed for the Pd–Pt alloy and H2-reducted PdO samples with respect to pure Pd samples can be explained in terms of the reduction in the lattice constant and interstitial volume due to the Pt addition, which determine a decrement of hydrogen atoms penetrating in the films. On the other hand, results show an improvement in time-response for Pd–Pt alloy and H2-reducted PdO films with respect to pure Pd ones, presumably due to the increase of its permeability to H2. Moreover, the sensing measurements repeated after 60 days show that the Pd–Pt alloy films, unlike the Pd-based ones, fully preserve their performances, demonstrating the advantage of the Pt inclusion for stability purposes when the samples are stored upon humidity.
Źródło:
Optica Applicata; 2020, 50, 4; 649-661
0078-5466
1899-7015
Pojawia się w:
Optica Applicata
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Seismic damage behaviour of aeolian sand reinforced concrete columns with I-shaped structural steel
Autorzy:
Wang, Y. H.
Wang, H. Y.
Wang, Y. P.
Zhao, M.
Qi, J.
Huo, G. Z.
Liu, P. Q.
Powiązania:
https://bibliotekanauki.pl/articles/1849772.pdf
Data publikacji:
2020
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
piasek eoliczny
słup żelbetowy
stal konstrukcyjna
przekrój I
zachowanie
uszkodzenie sejsmiczne
model uszkodzenia
aeolian sand
column
I-shaped steel
structural steel
seismic damage
behaviour
damage model
Opis:
To promote the application of aeolian sand resources for steel-concrete composite structures, an aeolian sand reinforced concrete column with I-shaped structural steel is proposed in this study. Four specimens are designed and manufactured with different replacement rates of aeolian sand. The seismic behaviour and damage evolution process of the specimens are studied by low-cycle repeated loading tests. Based on the test results, the mechanical characteristics, failure modes, hysteresis curves, skeleton curves, energy dissipation capacity, displacement ductility, and stiffness degradation of the specimens with different replacement rates of aeolian sand are analysed. In addition, the effects of the design parameters on the seismic behaviour of the specimens are also studied. The results show that the indexes of the seismic behaviour can be significantly improved by adding steel. Moreover, a revised damage model is proposed, to better reflect the evolution law of seismic damage of aeolian sand reinforced concrete columns with steel. The proposed model can provide an important reference for seismic damage assessment of the columns.
Źródło:
Archives of Civil Engineering; 2020, 66, 4; 633-650
1230-2945
Pojawia się w:
Archives of Civil Engineering
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
The Machine Learning Method of PIDVCA
Autorzy:
Li, L.
Wang, X.
Chen, G.
Powiązania:
https://bibliotekanauki.pl/articles/1841618.pdf
Data publikacji:
2020
Wydawca:
Uniwersytet Morski w Gdyni. Wydział Nawigacyjny
Tematy:
learning methods
personified intelligence
collision avoidance
dynamic collision avoidance
static collision avoidance
Opis:
Building a dynamic collision knowledge base of self-learning is one of the core contents of implementing "personified intelligence" in Personifying Intelligent Decision-making for Vessel Collision Avoidance (short for PIDVCA). In the paper, the machine learning method of PIDVCA combined with offline artificial learning and online machine learning is proposed. The static collision avoidance knowledge is acquired through offline artificial learning, and the isomeric knowledge representation integration method with process knowledge as the carrier is established, and the Dynamic collision avoidance knowledge is acquired through online machine learning guided by inference engine. A large number of simulation results show that the dynamic collision avoidance knowledge base constructed by machine learning can achieve the effect of anthropomorphic intelligent collision avoidance. It is verified by examples that the machine learning method of PIDVCA can realize target perception, target cognition and finally obtain an effective collision avoidance decision-making.
Źródło:
TransNav : International Journal on Marine Navigation and Safety of Sea Transportation; 2020, 14, 3; 533-540
2083-6473
2083-6481
Pojawia się w:
TransNav : International Journal on Marine Navigation and Safety of Sea Transportation
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Catalytic oxidation of lignin to aromatics over salen-porphyrin complex as a biomimetic catalyst
Autorzy:
Lu, X.-J.
Zhou, X.-F.
Zhu, Z.-L.
Sun, Y.
Tang, K.
Lei, F.-H.
Liu, Z.-G.
Wang, T.
Powiązania:
https://bibliotekanauki.pl/articles/52771.pdf
Data publikacji:
2019
Wydawca:
Sieć Badawcza Łukasiewicz - Instytut Technologii Drewna
Źródło:
Drewno. Prace Naukowe. Doniesienia. Komunikaty; 2019, 62, 203
1644-3985
Pojawia się w:
Drewno. Prace Naukowe. Doniesienia. Komunikaty
Dostawca treści:
Biblioteka Nauki
Artykuł
Wyświetlanie 1-15 z 138

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