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Tytuł:
The Obg subfamily of bacterial GTP-binding proteins: essential proteins of largely unknown functions that are evolutionarily conserved from bacteria to humans.
Autorzy:
Czyż, Agata
Węgrzyn, Grzegorz
Powiązania:
https://bibliotekanauki.pl/articles/1041458.pdf
Data publikacji:
2005
Wydawca:
Polskie Towarzystwo Biochemiczne
Tematy:
ribosomes
small GTP-binding proteins
gene expression
DNA repair
chromosomal functions
Opis:
Members of the Obg subfamily of small GTP-binding proteins (called Obg, CgtA, ObgE or YhbZ in different bacterial species) have been found in various prokaryotic and eukaryotic organisms, ranging from bacteria to humans. Although serious changes in phenotypes are observed in mutant bacteria devoid of Obg or its homologues, specific roles of these GTP-binding proteins remain largely unknown. Recent genetic and biochemical studies, as well as determination of the structures of Obg proteins from Bacillus subtilis and Thermus thermophilus, shed new light on the possible functions of the members of the Obg subfamily and may constitute a starting point for the elucidation of their exact biological role.
Źródło:
Acta Biochimica Polonica; 2005, 52, 1; 35-43
0001-527X
Pojawia się w:
Acta Biochimica Polonica
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
An introduction to DNA chips: principles, technology, applications and analysis.
Autorzy:
Gabig, Magdalena
Węgrzyn, Grzegorz
Powiązania:
https://bibliotekanauki.pl/articles/1044090.pdf
Data publikacji:
2001
Wydawca:
Polskie Towarzystwo Biochemiczne
Tematy:
gene expression
DNA array
oligonucleotide array
hybridisation
Opis:
This review describes the recently developed GeneChip technology that provides efficient access to genetic information using miniaturised, high-density arrays of DNA or oligonucleotide probes. Such microarrays are powerful tools to study the molecular basis of interactions on a scale that would be impossible using conventional analysis. The recent development of the microarray technology has greatly accelerated the investigation of gene regulation. Arrays are mostly used to identify which genes are turned on or off in a cell or tissue, and also to evaluate the extent of a gene's expression under various conditions. Indeed, this technology has been successfully applied to investigate simultaneous expression of many thousands of genes and to the detection of mutations or polymorphisms, as well as for their mapping and sequencing.
Źródło:
Acta Biochimica Polonica; 2001, 48, 3; 615-622
0001-527X
Pojawia się w:
Acta Biochimica Polonica
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
The Escherichia coli RNA polymerase α subunit and transcriptional activation by bacteriophage λ CII protein
Autorzy:
Gabig, Magdalena
Obuchowski, Michał
Ciesielska, Agnieszka
Latała, Barbara
Węgrzyn2, Alicja
Thomas, Mark
Węgrzyn, Grzegorz
Powiązania:
https://bibliotekanauki.pl/articles/1044881.pdf
Data publikacji:
1998
Wydawca:
Polskie Towarzystwo Biochemiczne
Źródło:
Acta Biochimica Polonica; 1998, 45, 1; 271-280
0001-527X
Pojawia się w:
Acta Biochimica Polonica
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Combined effect of stringent or relaxed response, temperature and rom function on the replication of pUC plasmids in Escherichia coli
Autorzy:
Herman, Anna
Węgrzyn, Alicja
Węgrzyn, Grzegorz
Powiązania:
https://bibliotekanauki.pl/articles/1045341.pdf
Data publikacji:
1994
Wydawca:
Polskie Towarzystwo Biochemiczne
Źródło:
Acta Biochimica Polonica; 1994, 41, 2; 122-124
0001-527X
Pojawia się w:
Acta Biochimica Polonica
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Characterization of conditions and determination of practical tips for mtDNA level estimation in various human cells
Autorzy:
Jędrak, Paulina
Sowa, Natalia
Barańska, Sylwia
Węgrzyn, Grzegorz
Powiązania:
https://bibliotekanauki.pl/articles/1038562.pdf
Data publikacji:
2017
Wydawca:
Polskie Towarzystwo Biochemiczne
Tematy:
mtDNA level
leukocytes
fibroblasts
qPCR methodology
Huntington disease
Opis:
Determination of mtDNA copy number in the cell is crucial to understand many cellular processes. Recently, the number of studies with the use of mitochondrial DNA (mtDNA) content as the determinant of mitochondrial abnormalities increased greatly and is still growing, therefore, optimization of technical conditions for this analysis is crucial. Despite using similar laboratory protocols, some results cannot be compared between research centers, thus causing discrepancies in the assessment of mtDNA content. The aim of this work was to test which conditions of biological sample collection and storage affect estimation of mtDNA level relative to the nuclear DNA (nDNA) in the blood samples and dermal fibroblasts. We found that the time and temperature of sample storage, as well as the type of the blood sample (whole blood or leukocytes) influence the estimate of mtDNA/nDNA ratio in the blood. In the case of dermal fibroblasts collected from healthy control and Huntington disease patients, our data indicate that the passage number of cells is essential to obtain reliable results.
Źródło:
Acta Biochimica Polonica; 2017, 64, 4; 699-704
0001-527X
Pojawia się w:
Acta Biochimica Polonica
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Clinical parameters of inflammatory bowel disease in children do not correlate with four common polymorphisms of the transforming growth factor β1 gene
Autorzy:
Liberek, Anna
Jakóbkiewicz-Banecka, Joanna
Kloska, Anna
Świderska, Joanna
Kmieć, Zbigniew
Łuczak, Grażyna
Wierzbicki, Piotr
Liberek, Tomasz
Marek, Krzysztof
Plata-Nazar, Katarzyna
Sikorska-Wiśniewska, Grażyna
Kamińska, Barbara
Węgrzyn, Grzegorz
Powiązania:
https://bibliotekanauki.pl/articles/1039871.pdf
Data publikacji:
2011
Wydawca:
Polskie Towarzystwo Biochemiczne
Tematy:
pediatric patients
gene polymorphism
Transforming growth factor β1
inflammatory bowel disease
Opis:
Transforming growth factor β1 (TGF-β1) is a cytokine affecting cell proliferation and development, which also has an immunomodulatory activity. Correlations between polymorphisms of the TGF-β1 gene and clinical parameters of inflammatory bowel disease (IBD) were reported previously in adults. Here, we tested whether such correlations occur in pediatric patients suffering from IBD. One hundred and four pediatric IBD patients were involved in this study. Among them, 36 were diagnosed with Crohn's Disease (CD) and 68 were diagnosed with ulcerative colitis (UC). The control group consisted of 103 children, in which IBD was excluded. TGF-β1 levels were determined in plasma and intestinal mucosa samples. The presence of the TGF β1 protein and the amount of TGF β1 mRNA were estimated in intestinal mucosa by immunohistochemistry and reverse transcription Real-Time PCR, respectively. Four common polymorphisms of the TGF-β1 gene were investigated: -800G/A, -509C/T, 869T/C and 915G/C. No significant correlation between TGF-β1 genotypes and (i) TGF-β1 levels in plasma and tissue samples, (ii) TGF-β1 gene expression efficiency in intestinal mucosa, (iii) IBD clinical parameters and (iv) inflammatory activity could be detected in children suffering from IBD. We conclude that, contrary to previous suggestions, the four common polymorphisms of the TGF-β1 gene do not influence the susceptibility to or clinical parameters of IBD in the tested population of children.
Źródło:
Acta Biochimica Polonica; 2011, 58, 4; 641-644
0001-527X
Pojawia się w:
Acta Biochimica Polonica
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Elektryczne biochipy
Electrical biochips
Autorzy:
Łoś, Marcin
Węgrzyn, Grzegorz
Powiązania:
https://bibliotekanauki.pl/articles/1198610.pdf
Data publikacji:
2005
Wydawca:
Polskie Towarzystwo Przyrodników im. Kopernika
Opis:
Rapid detection and quantification of microorganisms, particularly their nucleic acids and proteins, appears to be crucial in clinical practice, veterinary medicine, agriculture, basic research as well as in biotechnological factories. Although various techniques were described and are currently used, development of more rapid, more sensitive and quantitative methods seems to be still important. Here we describe a method for rapid detection of nucleic acids, proteins and small molecules, based on electrical biochip technology. This method is quick and sensitive. It can be thus expected that electrical bio-chips will soon be commonly used in biotechnology and medicine.
Źródło:
Kosmos; 2005, 54, 2-3; 175-182
0023-4249
Pojawia się w:
Kosmos
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Changes is genes coding for laccases 1 and 2 may contribute to deformation and reduction of wings in apollo butterfly (Parnassius apollo, Lepidoptera: Papilionidae) from the isolated population in Pieniny National Park (Poland)
Autorzy:
Łukasiewicz, Kinga
Węgrzyn, Grzegorz
Powiązania:
https://bibliotekanauki.pl/articles/1038862.pdf
Data publikacji:
2016
Wydawca:
Polskie Towarzystwo Biochemiczne
Tematy:
apollo butterfly
deformed wings
reduced wings
genes of laccases
Opis:
An isolated population of apollo butterfly (Parnassius apollo, Lepidoptera: Papilionidae) occurs in Pieniny National Park (Poland). Deformations and reductions of wings in a relatively large number of individuals from this population is found, yet the reasons for these defects are unknown. During studies devoted to identify cause(s) of this phenomenon, we found that specific regions of genes coding of enzymes laccases 1 and 2 could not be amplified from DNA samples isolated from large fractions of malformed insects while expected PCR products were detected in almost all (with one exception) normal butterflies. Laccases (p-diphenol:dioxygen oxidoreductases) are oxidases containing several copper atoms. They catalyse single-electron oxidations of phenolic or other compounds with concomitant reduction of oxygen to water. In insects, their enzymatic activities were found previously in epidermis, midgut, Malpighian tubules, salivary glands, and reproductive tissues. Therefore, we suggest that defects in genes coding for laccases might contribute to deformation and reduction of wings in apollo butterflies, though it seems obvious that deficiency in these enzymes could not be the sole cause of these developmental improperties in P. apollo from Pieniny National Park.
Źródło:
Acta Biochimica Polonica; 2016, 63, 1; 177-180
0001-527X
Pojawia się w:
Acta Biochimica Polonica
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Effect of coliphage λP gene mutations on the stability of the λO protein, theinitiator of λDNA replication
Autorzy:
Pawłowicz, Alicja
Węgrzyn, Grzegorz
Taylor, Karol
Powiązania:
https://bibliotekanauki.pl/articles/1045496.pdf
Data publikacji:
1993
Wydawca:
Polskie Towarzystwo Biochemiczne
Źródło:
Acta Biochimica Polonica; 1993, 40, 1; 29-31
0001-527X
Pojawia się w:
Acta Biochimica Polonica
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Differential effects of various soy isoflavone dietary supplements (nutraceuticals) on bacterial growth and human fibroblast viability
Autorzy:
Pierzynowska, Karolina
Rzeszótko, Agata
Blendowska, Aleksandra
Wieczerzak, Ewa
Rodziewicz-Motowidło, Sylwia
Piotrowska, Ewa
Węgrzyn, Grzegorz
Powiązania:
https://bibliotekanauki.pl/articles/1038411.pdf
Data publikacji:
2018
Wydawca:
Polskie Towarzystwo Biochemiczne
Tematy:
genistein
soy isoflavone extracts
anti-menopausal dietary supplements
bacterial growth
viability of human cells
Opis:
Flavonoids, polyphenolic compounds present in many food products, affect growth of different bacterial species when tested as purified or synthetic substances. They can also influence gene expression in human cells, like fibroblasts. Here, we asked if soy isoflavone extracts, commonly used in many products sold as anti-menopausal dietary supplements, influence bacterial growth similarly to a synthetic isoflavone, genistein. Four commercially available products were tested in amounts corresponding to genistein concentrations causing inhibition of growth of Vibrio harveyi (a model bacterium sensitive to this isoflavone) and Escherichia coli (a model bacterium resistant to genistein). Differential effects of various extracts on V. harveyi and E. coli growth, from stimulation, to no changes, to inhibition, were observed. Moreover, contrary to genistein, the tested extracts caused a decrease (to different extent) in viability of human dermal fibroblasts. These results indicate that effects of various soy isoflavone extracts on bacterial growth and viability of human cells are different, despite similar declared composition of the commercially available products.
Źródło:
Acta Biochimica Polonica; 2018, 65, 2; 325-332
0001-527X
Pojawia się w:
Acta Biochimica Polonica
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Methylxanthines (caffeine, pentoxifylline and theophylline) decrease the mutagenic effect of daunomycin, doxorubicin and mitoxantrone
Autorzy:
Piosik, Jacek
Gwizdek-Wiśniewska, Anna
Ulanowska, Katarzyna
Ochociński, Jakub
Czyż, Agata
Węgrzyn, Grzegorz
Powiązania:
https://bibliotekanauki.pl/articles/1041344.pdf
Data publikacji:
2005
Wydawca:
Polskie Towarzystwo Biochemiczne
Tematy:
Vibrio harveyi mutagenicity assay
mitoxantrone
doxorubicin
daunomycin
xanthines
Opis:
Previously performed experiments showed that methylxanthines, especially caffeine, may protect cells against cytostatic or cytotoxic effects of several aromatic compounds. One of the proposed mechanisms of this protection is based on stacking interactions between π electron systems of polycyclic aromatic molecules. In this work, we demonstrate that caffeine and other methylxanthines - pentoxifylline and theophylline - significantly decrease mutagenicity of the anticancer aromatic drugs daunomycin, doxorubicin and mitoxantrone. The spectrophotometric titration of these aromatic compounds by methylxanthines indicated formation of mixed aggregates. The concentrations of free active forms of the drugs decreased when the concentrations of methylxanthines increased in the mixture. Therefore, likely methylxanthines may play a role of scavengers of the free active forms of daunomycin, doxorubicin and mitoxantrone.
Źródło:
Acta Biochimica Polonica; 2005, 52, 4; 923-926
0001-527X
Pojawia się w:
Acta Biochimica Polonica
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Staphylococcus aureus as an infectious agent: overview of biochemistry and molecular genetics of its pathogenicity
Autorzy:
Plata, Konrad
Rosato, Adriana
Węgrzyn, Grzegorz
Powiązania:
https://bibliotekanauki.pl/articles/1040468.pdf
Data publikacji:
2009
Wydawca:
Polskie Towarzystwo Biochemiczne
Tematy:
Staphylococcus aureus
virulence
pathogenicity genes
toxins
methicillin resistance
Opis:
Although it is estimated that 20-30% of the general human population are carriers of Staphylococcus aureus, this bacterium is one of the most important etiological agents responsible for healthcare-associated infections. The appearance of methicillin resistant S. aureus (MRSA) strains has created serious therapeutical problems. Detailed understanding of the mechanisms of S. aureus infections seems necessary to develop new effective therapies against this pathogen. In this article, we present an overview of the biochemical and genetic mechanisms of pathogenicity of S. aureus strains. Virulence factors, organization of the genome and regulation of expression of genes involved in virulence, and mechanisms leading to methicilin resistance are presented and briefly discussed.
Źródło:
Acta Biochimica Polonica; 2009, 56, 4; 597-612
0001-527X
Pojawia się w:
Acta Biochimica Polonica
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Improved HPLC method for total plasma homocysteine detection and quantification
Autorzy:
Sawuła, Wojciech
Banecka-Majkutewicz, Zyta
Kadziński, Leszek
Jakóbkiewicz-Banecka, Joanna
Węgrzyn, Grzegorz
Nyka, Walenty
Banecki, Bogdan
Powiązania:
https://bibliotekanauki.pl/articles/1040824.pdf
Data publikacji:
2008
Wydawca:
Polskie Towarzystwo Biochemiczne
Tematy:
homocysteine measurement
stroke risk factors
HPLC
validation
Opis:
Recent clinical research has pointed at hyperhomocysteinemia as an independent risk factor in a number of cardiovascular and neurological diseases. We have improved a chromatographic method of total plasma homocysteine measurements in order to obtain higher sensitivity, reliability and reproducibility. The method demonstrates excellent linearity (R = 0.999), range (< 2-100 µM), precision (instrumental RSD 0.06 and method RSD 1.17), accuracy (recovery of 99.92 and RSD 1.27), reproducibility, quantification limit and ruggedness (e.g. pH from 2.0 to 2.5). Because even a small increase in homocysteine level can be a significant risk factor of cardiovascular diseases, such a precise method is required. The constructed method allows the measurement of plasma pyridoxal phosphate, PLP, the co-enzyme form of vitamin B6, on the same column and similar reagents. The developed method has been successfully applied to measure both total plasma and serum homocysteine in a group of acute stroke patients.
Źródło:
Acta Biochimica Polonica; 2008, 55, 1; 119-126
0001-527X
Pojawia się w:
Acta Biochimica Polonica
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
A method for isolation of plasmid DNA replication intermediates from unsynchronized bacterial cultures for electron microscopy analysis
Autorzy:
Śrutkowska, Sylwia
Konopa, Grażyna
Węgrzyn, Grzegorz
Powiązania:
https://bibliotekanauki.pl/articles/1044875.pdf
Data publikacji:
1998
Wydawca:
Polskie Towarzystwo Biochemiczne
Źródło:
Acta Biochimica Polonica; 1998, 45, 1; 233-240
0001-527X
Pojawia się w:
Acta Biochimica Polonica
Dostawca treści:
Biblioteka Nauki
Artykuł

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