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Wyszukujesz frazę "Volkov, M.E." wg kryterium: Autor


Wyświetlanie 1-4 z 4
Tytuł:
Insecticidal activity of three 10–12 nucleotides long antisense sequences from 5.8S ribosomal RNA gene of gypsy moth Lymantria dispar L. against its larvae
Autorzy:
Oberemok, V.V.
Laikova, K.V.
Useinov, R.Z.
Gal`chinsky, N.V.
Novikov, I.A.
Yurchenko, K.A.
Volkov, M.E.
Gorlov, M.V.
Brailko, V.A.
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Powiązania:
https://bibliotekanauki.pl/articles/2084849.pdf
Data publikacji:
2019
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
antisense oligonucleotides
DNA insecticides
gypsy moth
insect pest control
Lymantria dispar
5.8S ribosomal RNA
Opis:
5.8S ribosomal RNA plays an important role in protein synthesis and eukaryotic ribosome translocation. Contact DNA insecticides based on antisense fragments of 5.8S ribosomal RNA gene of gypsy moth Lymantria dispar L. showed prospective insecticidal activity on its larvae. The most pronounced insecticidal effect was found for antisense fragments 10 and 11 nucleotides long (oligoRIBO-10 and oligoRIBO-11), whereas 12 nucleotides long fragment (oligoRIBO-12) caused the lowest level of insect mortality. This data corresponds to results obtained earlier using rabbit reticulocyte and wheat germ extracts, where maximum inhibition of protein synthesis was observed when a relevant oligomer 10-11 nucleotides long was used, whilst longer chain lengths resulted in reduced inhibition. Using oligoRIBO-11 fragment we have shown penetration of antisense oligonucleotides to insect cells through insects’ exoskeletons. MALDI technique registered the penetration of the oligoRIBO-11 fragment into insect cells after 30 min and a significant response of insect cells to the applied oligonucleotide after 60 min, which indicates not only that the oligonucleotide enters the insect cells, but also the synthesis of new substances in response to the applied DNA fragment. Contact DNA insecticides developed from the L. dispar 5.8S ribosomal RNA gene provide a novel biotechnology for plant protection using unmodified antisense oligonucleotides.
Źródło:
Journal of Plant Protection Research; 2019, 59, 4; 561-564
1427-4345
Pojawia się w:
Journal of Plant Protection Research
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Environmental status of the Arctic soils
Autorzy:
Nikitina, M.
Popova, L.
Korobitcina, J.
Efremova, O.
Trofimova, A.
Nakvasina, E.
Volkov, A.
Powiązania:
https://bibliotekanauki.pl/articles/15937.pdf
Data publikacji:
2015
Wydawca:
Uniwersytet Warmińsko-Mazurski w Olsztynie / Polskie Towarzystwo Magnezologiczne im. Prof. Juliana Aleksandrowicza
Tematy:
environmental status
Arctic soil
soil
agrochemical parameter
heavy metal
Opis:
The Arctic region is under an ever-increasing anthropogenic influence. The nature in the Arctic is extremely vulnerable to the effects of pollutants, which may eventually lead to certain irreversible ecological processes. Heavy metals occupy a special place among priority pollutants. Therefore, the aim of this study has been to assess the ecological status and the degree of contamination with heavy metals of the soils of Arctic and sub-Arctic areas. Arctic soils were studied in the region of the White Sea and the Barents Sea during the Arctic Floating University, a research expedition held in July 2012 by M. Lomonosov Northern (Arctic) Federal University. During the expedition, samples were collected and the landscapes (topography, vegetation) were described. In order to assess the impact of environmental contamination, the total content of heavy metals in soils was evaluated by X-ray fluorescence analysis (XRF). The level of soil contamination was assessed according to the maximum permissible concentrations ( MPC), temporary permissible concentrations (TPC), chemical substance factor (Kc) and the total pollution index (Zc). Our analysis of the experimental data showed a wide-range change in the soil agrochemical parameters. It has been demonstrated that the investigated soils of the Arctic and sub-Arctic areas represent different soil properties, are affected by different climatic conditions and belong to different texture taxonomic classes, e.g. the grain-size distribution varies from sand to clay loam; the pH ranges from strongly acid to neutral and the content of organic substances can be very low to high. According to the total pollution index, almost all the soils present an acceptable heavy metal contamination level. In most of the studied soils, the heavy metals in terms of amounts accumulated can be ordered as follows: Co > Pb > Cu > Zn > As > Ni > V > Mn.
Źródło:
Journal of Elementology; 2015, 20, 3
1644-2296
Pojawia się w:
Journal of Elementology
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
The Microstructure and Fatigue of Ultrafine-Grained Al-Cu-Mg Alloy
Autorzy:
Islamgaliev, R.
Khafizova, E.
Kulyasova, O.
Nesterov, K.
Ganeev, A.
Golubovskiy, E.
Volkov, M.
Powiązania:
https://bibliotekanauki.pl/articles/1402104.pdf
Data publikacji:
2015-10
Wydawca:
Polska Akademia Nauk. Instytut Fizyki PAN
Tematy:
61.05.J-
81.05.Bx
Opis:
In this work the influence of equal-channel angular pressing on strength and fatigue of an aluminum alloy has been studied. Transmission electron microscopy was applied to determine an average grain size, shape, and size of precipitates. The ultimate tensile strength and fatigue endurance limit of ultrafine-grained and coarse-grained samples were evaluated at 20°C and 175°C.
Źródło:
Acta Physica Polonica A; 2015, 128, 4; 633-636
0587-4246
1898-794X
Pojawia się w:
Acta Physica Polonica A
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Some problems of plasma-material interactions in fusion devices
Autorzy:
Glazunov, G.
Volkov, E.
Lapshin, V.
Tereshin, V.
Sadowski, M.
Langner, J.
Stanisławski, J.
Powiązania:
https://bibliotekanauki.pl/articles/146712.pdf
Data publikacji:
2000
Wydawca:
Instytut Chemii i Techniki Jądrowej
Tematy:
boron-carbide erosion
plasma-material interaction
TiN-coated stainless-steel
Opis:
The paper concerns some questions connected with the interaction of high-temperature plasma-ion fluxes with solid materials inside experimental chambers used for fusion-oriented research, and particularly inside the Torsatron-type facilities. Mechanisms of the boron carbide erosion are described and discussed. Also described is the behaviour of TiN-coated stainlesssteel surfaces under the irradiation with hydrogen plasma-ion fluxes. In summary, there are described damages in the boron carbide layers and changes in the composition of the TiN-coatings.
Źródło:
Nukleonika; 2000, 45, 3; 209-213
0029-5922
1508-5791
Pojawia się w:
Nukleonika
Dostawca treści:
Biblioteka Nauki
Artykuł
    Wyświetlanie 1-4 z 4

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