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Wyszukujesz frazę "Ustiatik, Reni" wg kryterium: Autor


Wyświetlanie 1-2 z 2
Tytuł:
Siderophore Production of the Hg-Resistant Endophytic Bacteria Isolated from Local Grass in the Hg-Contaminated Soil
Autorzy:
Ustiatik, Reni
Nuraini, Yulia
Suharjono, -
Handayanto, Eko
Powiązania:
https://bibliotekanauki.pl/articles/1838397.pdf
Data publikacji:
2021
Wydawca:
Polskie Towarzystwo Inżynierii Ekologicznej
Tematy:
endophytic bacteria
growth promoter
metal chelator
siderophore production
Hg phytoremediation
Opis:
Mercury (Hg)-contaminated soil remediation has become an urgent necessity due to its harmful effect on the environment and living organisms. The use of plant-endophyte partnership for phytoremediation demonstrates an excellent opportunity for cleaning heavy metal contaminated soil. This study aimed to screen and characterize the phenotype of the Hg-resistant endophytic bacteria from local grasses (Cynodon dactylon and Eleusine indica) in the Hg-contaminated soil of West Nusa Tenggara, Indonesia with siderophore-producing traits. Siderophore production of bacteria was qualitatively tested using overlay-chrome azurol S (O-CAS) medium and quantitatively tested using the succinic acid medium. The assay was designed using a Completely Randomized Factorial Design consisting of two factors, i.e., isolate type and incubation time with three replicates. The selected isolates were pathogenicity tested, then they were phenotypically characterized. All tested isolates showed a positive result on changing O-CAS medium color from blue to yellow/brown that indicated hydroxamate type of siderophore. The highest siderophore production was achieved at 72 hours of incubation, by the EI5 and EI6 isolates (62.90% and 35.31%, respectively). In turn, the CD6, EI5 and EI6 isolates achieved high siderophore production at a short incubation period (48 hours). However, during the hemolysis test, only the CD6 and EI6 isolates were not pathogenic. The CD6 and EI6 isolates would be used for phytoremediation on Hg-contaminated soil in the future study. On the basis of the 16S rDNA analysis, it was shown that the CD6 isolate was Jeotgalicoccus huakuii and the EI6 isolate was Bacillus amyloliquefaciens.
Źródło:
Journal of Ecological Engineering; 2021, 22, 5; 129-138
2299-8993
Pojawia się w:
Journal of Ecological Engineering
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Changes in Essential Soil Nutrients and Soil Disturbance Directly Affected Soil Microbial Community Structure: A Metagenomic Approach
Autorzy:
Wiryawan, Adam
Eginarta, Wahyu Satria
Hermanto, Feri Eko
Ustiatik, Reni
Dinira, Layta
Mustafa, Irfan
Powiązania:
https://bibliotekanauki.pl/articles/2173263.pdf
Data publikacji:
2022
Wydawca:
Polskie Towarzystwo Inżynierii Ekologicznej
Tematy:
forest soil
metagenome
soil bacteria
soil nutrients
tilled land
Opis:
Soil environment, both biotic (e.g., microbial community) and abiotic (e.g., nutrients and water availability) factors determine soil fertility and health and are directly affected by soil management systems. However, only limited studies evaluate the combined effect of nutrients availability and soil disturbance on the soil bacteria community structure, especially in conventional agricultural practices, on the forests converted to agricultural land. This study aimed to provide a viewpoint of the effect of different soil management systems, i.e., forest soil (natural process) and tilled land, on soil bacteria community structure on forest converted to agricultural land, according to a metagenomics approach. Moreover, each land use was sampled to identify the bacterial community using 16S gene as a biomarker. The sequencing was performed using MinION (Oxford Nanopore Technologies) to read the DNA sequence from each soil sample. Principle Component Analysis (PCA) was performed to comprehend the relationship between availability of nutrients and bacterial diversity. The results revealed that the concentrations of soil micronutrients, such as iron, zinc, and magnesium, were significantly higher in forest soil than in tilled land. According to diversity indices, soil bacteria were more diverse in forest soil than in tilled land. Forest soil had more distinctive taxa than tilled land. Several species comprised the most abundant taxa, such as Candidatus Koribacter versatilis, Candidatus Solibacter usiatus, Rhodoplanes sp., Luteitalea pratensis, and Betaproteobacteria bacterium, were more scarce in tilled land. On the distinctive taxa in each soil sample, Anseongella ginsenosidimutans and Janthinobacterium sp. were the most abundant species in forest and tilled land, respectively. According to PCA analysis, soil management system affected the soil micro-and macronutrients also microbial community structure between forest and tilled land. In conclusion, soil management influences the essential nutrient content and bacterial community structure of soil. Better management should be adopted to maintain soil quality near forest soil.
Źródło:
Journal of Ecological Engineering; 2022, 23, 7; 238--245
2299-8993
Pojawia się w:
Journal of Ecological Engineering
Dostawca treści:
Biblioteka Nauki
Artykuł
    Wyświetlanie 1-2 z 2

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