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Wyszukujesz frazę "Urban-Chmiel, R." wg kryterium: Autor

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    Wyświetlanie 1-9 z 9
    Tytuł:
    Pterofagia oraz kanibalizm jako następstwa obniżonego poziomu dobrostanu u drobiu
    Feather pecking and cannibalism - the consequences of reduced welfare in poultry
    Autorzy:
    Urban-Chmiel, R.
    Powiązania:
    https://bibliotekanauki.pl/articles/861206.pdf
    Data publikacji:
    2014
    Wydawca:
    Krajowa Izba Lekarsko-Weterynaryjna
    Tematy:
    drob
    chow zwierzat
    dobrostan zwierzat
    zachowanie sie zwierzat
    pogorszenie dobrostanu
    zaburzenia zachowania
    pterofagia
    kanibalizm
    przyczyny
    czynniki genetyczne
    czynniki srodowiska
    warunki utrzymania zwierzat
    bledy zywieniowe
    pasozyty zwierzat
    zapobieganie
    Źródło:
    Życie Weterynaryjne; 2014, 89, 09
    0137-6810
    Pojawia się w:
    Życie Weterynaryjne
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    An electrophoretic characterization of iron-transporting proteins in Mannheimia haemolytica A1
    Autorzy:
    Puchalski, A.
    Urban-Chmiel, R.
    Dec, M.
    Wernicki, A.
    Powiązania:
    https://bibliotekanauki.pl/articles/30623.pdf
    Data publikacji:
    2013
    Wydawca:
    Polska Akademia Nauk. Czytelnia Czasopism PAN
    Opis:
    Iron-regulated outer membrane proteins (IROMPs) in Mannheimia haemolytica A1, which function as a receptor for complexes containing iron ions, are induced by iron deficiency in the growth environment of the bacteria. Densitometric analysis of SDS-PAGE separation showed expression of IROMPs of 71, 77, and 100 kDa in the case of bacteria grown in a medium with 2,2-dipyridyl. The electrophoregrams obtained in 2-DE separations confirmed the presence of protein fractions with these molecular weights and isoelectric points ranging from 5.4 to 6.4. The results of the study also confirmed the ability of M. haemolytica A1 proteins involved in iron uptake to induce a protective immune response. In Western blot with serum from convalescent calves naturally infected with M. haemolytica A1, distinct reactions were obtained for IROMPs of 71, 77, and 100 kDa.
    Źródło:
    Polish Journal of Veterinary Sciences; 2013, 16, 3
    1505-1773
    Pojawia się w:
    Polish Journal of Veterinary Sciences
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Application of loop-mediated isothermal amplification (LAMP) assays for the detection of bovine herpesvirus 1
    Autorzy:
    Socha, W.
    Rola, J.
    Urban-Chmiel, R.
    Żmudziński, J.F.
    Powiązania:
    https://bibliotekanauki.pl/articles/2087889.pdf
    Data publikacji:
    2017
    Wydawca:
    Polska Akademia Nauk. Czytelnia Czasopism PAN
    Źródło:
    Polish Journal of Veterinary Sciences; 2017, 3; 619-622
    1505-1773
    Pojawia się w:
    Polish Journal of Veterinary Sciences
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Oxidative parameters and expression of 70kDa heat shock proteins in pig heart tissue after transport and slaughter
    Autorzy:
    Urban-Chmiel, R.
    Pyz-Lukasik, R.
    Dudzic, A.
    Wernicki, A.
    Powiązania:
    https://bibliotekanauki.pl/articles/31697.pdf
    Data publikacji:
    2014
    Wydawca:
    Polska Akademia Nauk. Czytelnia Czasopism PAN
    Opis:
    In view of the significant role of Hsp70 in protecting the organism against the destructive effects of stress, and the possibility of using this protein as a marker of the infarction process in the heart, the aim of this study was to conduct an evaluation of the expression of 70kDa heat shock proteins (Hsp70) and the concentration of TBARS (thiobarbituric acid reactive substances) and nitric oxide ions (NO), determined as nitrite ions, as markers of oxidative stress in hearts obtained from healthy pigs following slaughter and pigs which had died during or immediately after transport with symptoms of sudden cardiac death. The material consisted of hearts obtained from 90 pigs following slaughter and from pigs which had died. Oxidative stress was determined in heart lysates based on the concentration of TBARS and nitrite ions. Expression and concentration of Hsp70 were determined using SDS-PAGE, Western blotting, and ELISA. Expression of Hsp70 was observed in hearts lysates obtained from slaughtered pigs and from those which had died with symptoms of sudden death. The strongest reaction in the Western Blotting was noted in hearts lysates from pigs with no pathological changes. The highest TBARS concentration was observed in lysates from hearts in pigs which had died during or immediately after transport. The highest concentration of NO ions, determined as nitrite ions, was noted in hearts from pigs with myocardial infarction lesions. The significant decrease observed in Hsp70 concentration in heart tissue obtained from the pigs which had died in comparison to the hearts from healthy pigs indicates the important role of this protein in protecting the heart muscle against the destructive effects of stress, which limits the occurrence of post-stress cardiomyopathy in pigs following transport.
    Źródło:
    Polish Journal of Veterinary Sciences; 2014, 17, 3
    1505-1773
    Pojawia się w:
    Polish Journal of Veterinary Sciences
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Assessment of lipid and protein peroxidation markers in non-pregnant and pregnant female dogs
    Autorzy:
    Szczubial, M.
    Kankofer, M.
    Dabrowski, R.
    Bochniarz, M.
    Urban-Chmiel, R.
    Powiązania:
    https://bibliotekanauki.pl/articles/2087973.pdf
    Data publikacji:
    2015
    Wydawca:
    Polska Akademia Nauk. Czytelnia Czasopism PAN
    Opis:
    The aim of the study was to investigate oxidative stress during normal pregnancy in female dogs based on an evaluation of plasma markers for lipid and protein peroxidation. Twenty clinically healthy female dogs (10 non-pregnant and 10 pregnant) were used in the study. Blood samples from the pregnant animals were collected at 19-21, 38-40, and 56-58 days of pregnancy. Blood samples from non-pregnant female dogs were obtained between 20 and 35 days after ineffective breeding. As indicators of oxidative stress, we measured the following using spectrophotometric and spectrof-luorimetric methods: thiobarbituric acid reactive substances (TBARS), radical cations of N,N, die-thylpara-phenylene diamine (RC-DEPPD), sulfhydryl groups (SH groups), bityrosine and formyl-kynurenine. The mean plasma TBARS concentration in the pregnant dogs (0.486 ± 0.071 - 0.581 ± 0.191 |imol/g protein) was significantly higher (p<0.05) than that found in the non-pregnant animals (0.274 ± 0.111 |imol/g protein). A marked, although not significant, decrease in SH group content, as well as an increase in bityrosine and formylkynurenine concentration were concurrently observed in the pregnant dogs. No significant differences were found in terms of the studied markers in the pregnant animals when comparing the values obtained during the investigated periods of pregnancy, although there was a progressive decrease in TBARS concentration and a progressive increase in RC-DEPPD, bityrosine and formylkynurenine contents. Our findings suggest that normal pregnancy in female dogs is associated with oxidative stress. Further studies are necessary to establish the physiological ranges of antioxidative/oxidative profiles in pregnant dogs and to explain if and how the intensity of oxidative stress might contribute to the risk of the complications of pregnancy.
    Źródło:
    Polish Journal of Veterinary Sciences; 2015, 18, 1
    1505-1773
    Pojawia się w:
    Polish Journal of Veterinary Sciences
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    The influence of selected techniques of bovine leukocyte isolation on their viability and metabolism
    Autorzy:
    Urban-Chmiel, R.
    Lisiecka, U.
    Chlopas, A.
    Kurek, L.
    Wernicki, A.
    Powiązania:
    https://bibliotekanauki.pl/articles/30570.pdf
    Data publikacji:
    2011
    Wydawca:
    Polska Akademia Nauk. Czytelnia Czasopism PAN
    Opis:
    The aim of the study was to assess the effect of selected isolation methods on the viability and metabolism of bovine leukocytes. The cells were isolated using a Ficoll 1077, Histopaque 1083 gradient and osmotic shock method, and Ficoll or Histopaque with osmotic shock. Evaluation were made of the total number of cells, viability after isolation and in 24h culture on RPMI 1640 medium and metabolism with NBT reduction assay. Microscopic and cytometric evaluation of the leukocytes revealed that the isolation methods applied had an influence on their number and viability. Based on the results it can be concluded that isolation methods of cells in a Histopaque or Ficoll yield highly pure cell fractions with high viability.
    Źródło:
    Polish Journal of Veterinary Sciences; 2011, 14, 4
    1505-1773
    Pojawia się w:
    Polish Journal of Veterinary Sciences
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Effect of conglutinin on phagocytic activity of bovine granulocytes
    Autorzy:
    Dec, M.
    Wernicki, A.
    Puchalski, A.
    Urban-Chmiel, R.
    Radej, S.
    Powiązania:
    https://bibliotekanauki.pl/articles/31860.pdf
    Data publikacji:
    2012
    Wydawca:
    Polska Akademia Nauk. Czytelnia Czasopism PAN
    Tematy:
    conglutinin
    phagocytic activity
    bovine granulocyte
    granulocyte
    chemotaxis
    leucocyte
    cattle
    Opis:
    In the present study we investigated the effect of bovine conglutinin on the phagocytic activity of leukocytes. We measured both the chemotactic activity of conglutinin and its effect on the internalization of zymosan particles and E. coli by granulocytes. We also assessed the binding of conglutinin to various microorganisms isolated from clinical cases in cattle. We showed that conglutinin binds strongly to the surface of yeast cells and to mannan-rich zymosan particles, while weak binding was observed in the case of the bacterial strains tested, including those whose O antigen is composed of mannan. Conglutinin (1-10 μg/ml) neither acts as a chemotactic factor for peripheral blood leukocytes nor affects ingestion of E. coli by granulocytes. However, as flow cytometry based assay showed, conglutinin (0.1-1 μg/ml) increased ingestion of zymosan expressed as mean fluorescence intensity (MFI) of positive cells.
    Źródło:
    Polish Journal of Veterinary Sciences; 2012, 15, 3
    1505-1773
    Pojawia się w:
    Polish Journal of Veterinary Sciences
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Characterization of outer membrane proteins participating in iron transport in Pasteurella multocida serotype A3
    Autorzy:
    Puchalski, A
    Dec, M.
    Wernicki, A.
    Urban-Chmiel, R.
    Gieral, A.
    Powiązania:
    https://bibliotekanauki.pl/articles/30100.pdf
    Data publikacji:
    2010
    Wydawca:
    Polska Akademia Nauk. Czytelnia Czasopism PAN
    Tematy:
    iron-regulated outer membrane protein
    Pasteurella multocida
    iron transport
    iron ion
    iron deficiency
    protein
    new protein
    bacterial growth
    standard condition
    molecular weight
    amino acid sequence
    Opis:
    Iron-regulated outer membrane proteins (IROMPs) of P. multocida serotype A3, which function as receptors for complexes containing iron ions, are induced by iron deficiency in the bacterial growth environment. Analysis of an electrophoresis image of proteins isolated from bacteria grown on medium supplemented with 2,2'-dipyridyl revealed expression of 16 new proteins that were not noted in the case of the bacteria grown in standard conditions, with molecular weights from 30 to 160 kDa. Induction of IROMP expression occurred within 30 minutes after restricted iron conditions were established. In immunoblotting, distinct reactions were noted for proteins of molecular weight ranges of 25-49 kDa, 61-95 kDa, and 108-214 kDa. Proteins of the molecular weight of 68, 75 and 86 kDa were analysed using mass spectrometry and matched with the highest probability to proteins in the NCBI data base. Several dozen different proteins with similar amino acid sequences were matched to each sample.
    Źródło:
    Polish Journal of Veterinary Sciences; 2010, 13, 1; 121-127
    1505-1773
    Pojawia się w:
    Polish Journal of Veterinary Sciences
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Detection of the bovine viral diarrhoea virus (BVDV) in young beef cattle in eastern and south-eastern regions of Poland
    Autorzy:
    Wernicki, A.
    Urban-Chmiel, R.
    Stegierska, D.
    Adaszek, L.
    Kalinowski, M.
    Puchalski, A.
    Dec, M.
    Powiązania:
    https://bibliotekanauki.pl/articles/30337.pdf
    Data publikacji:
    2015
    Wydawca:
    Polska Akademia Nauk. Czytelnia Czasopism PAN
    Opis:
    In view of the scarcity of information concerning viral diarrhoea virus (BVDV) infections in beef cattle in Poland, the aim of this study was to evaluate the presence of the BVDV in young beef cattle from selected herds in eastern and south-eastern regions of Poland. The material consisted of 78 sera obtained from beef cattle from 15 farms, aged 6-12 months. The anti-BVDV antibody level in the sera was estimated with an ELISA kit, and detection of the BVDV was carried out by standard PCR and one step Real-Time RT-PCR. The ELISA results showed a high degree (80%) of positivity in 5 of the 78 samples. In 7 samples the degree of positivity was in the very low range: <40%. Of the 78 cDNA samples, the presence of genetic material with a length of 288 bp was found by standard PCR in 3 sera. The genetic material of BVDV was also found in the sera of the same three calves by Real-Time HRM PCR. BVDV infection in young beef cattle in south-eastern Poland is not a significant problem. This was confirmed by the positive ELISA results for 6.4% of the animals and the positive PCR results for 3.9%. The percentage of positive beef herds was about 8.6%. However, due to the severe nature of the disease and rapid transmission of the virus, regular monitoring of BVDV should be carried out.
    Źródło:
    Polish Journal of Veterinary Sciences; 2015, 18, 1
    1505-1773
    Pojawia się w:
    Polish Journal of Veterinary Sciences
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
      Wyświetlanie 1-9 z 9

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