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    Wyświetlanie 1-3 z 3
    Tytuł:
    Influence of change in the proportion of H1 histone variants on microsporogenesis and development of male gametophyte in transgenic plants of tobacco [Nicotiana tabacum L.]
    Autorzy:
    Slusarczyk, J
    Wierzbicki, A.
    Przewloka, M.
    Tykarska, T.
    Jerzmanowski, A.
    Kuras, M.
    Powiązania:
    https://bibliotekanauki.pl/articles/57064.pdf
    Data publikacji:
    2003
    Wydawca:
    Polskie Towarzystwo Botaniczne
    Tematy:
    sterile pollen grain
    male
    development
    transgenic plant
    gametophyte
    tobacco
    microsporogenesis disorder
    histone H1
    Nicotiana tabacum
    Opis:
    As continuation of investigations in to the mechanism of the role of the H1 histone, which is a crucial protein component chromosomes of all eukaryotes, transgenic tobacco plants with different levels of the H1 histone variants were examined. Tobacco has six sequential variants of the H1 histone: two major ones (H1A and H1B), constituting ca. 90% of all H1, and four minor ones (H1C, H1D, H1E and H1F), occurring in very small quantities. The following groups of plants were examined: K - control group with a full set of histone variants; -AB -with the A and B variants removed; -ABCD - with the A, B, C and D variants removed; and -CD - with the C and D variants removed. The analysis of microsporogenesis in those plants, based on preparations squeezed in acetoorcein, revealed the asynchronous course of meiosis in -AB and -ABCD plants, occurrence of chromosomal aberration, and, consequently, the formation of sterile pollen grains (accordingly: 84,4% and 81,4%). In -CD plants, the percentage of aberration and sterile pollen grains was similar to the control material. Electron microscope observations of microsporogenesis showed ultrastructural changes. In -AB and -ABCD plants, a major portion of the pollen grains were degraded. The smallest number of degraded pollen grains, in comparison with the control, was found in the -CD group.
    Źródło:
    Acta Societatis Botanicorum Poloniae; 2003, 72, 1
    0001-6977
    2083-9480
    Pojawia się w:
    Acta Societatis Botanicorum Poloniae
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Structural and ultrastructural study of Capsicum annuum leaves after treatment with Uncaria tomentosa bark extracts
    Autorzy:
    Tykarska, T
    Gulewicz, K.
    Zobel, A.
    Augustynowicz, J.
    Zurowska, K.
    Kuras, M.
    Powiązania:
    https://bibliotekanauki.pl/articles/56812.pdf
    Data publikacji:
    2003
    Wydawca:
    Polskie Towarzystwo Botaniczne
    Tematy:
    uncaria tomentosa
    structure
    extract
    Capsicum annuum
    treatment
    greenhouse condition
    leaf
    ultrastructure
    plant
    Opis:
    The influence of an Uncaria tomentosa extract on the development of Capsicum plants grown in green-house conditions was examined. The effect of the treatment was investigated with microscopic techniques (light and electron microscope) in leaves from three levels of control plants and plants after treatment with the extract added to the soil in doses of 0.4 and 16 mg/ml (200 ml per pot/plant). In control leaves, changes typical of the subsequent phases of normal development were observed: nuclear chromatin became slightly condensed, plastoglobuli of chloroplasts increased in number and size, intragranal thylakoids were somewhat dilatated. In addition to such commonly occurring changes, some symptoms typical of pepper were observed in the ontogenesis of the examined plant: an increased number of spherical electron-dense deposits in vacuoles, an increased number of peroxisomes, the occurrence of numerous paracrystalline structures in chloroplasts of mature leaves, and, starting in mature leaves, expulsion of plastoglobuli from chloroplasts. After the treatment, most of those changes, leading to ageing, occurred much earlier and were more distinct. Chloroplasts, already in the youngest examined leaves, showed dilatation of intergranal thylakoids, which intensified with aging of the leaves and degradation of grana in the oldest leaves. Starch grains decreased in size and number and plastoglobuli became large. Vesiculation of ground cytoplasm in all leaves was stronger than in the control. No paracrystalline structures in chloroplasts or expulsion of plastoglobuli were observed. Another unusual phenomenon was the disappearance of spherical electron-dense deposits in the central vacuoles of cells. Those observations suggested that U. tomentosa extract enhanced the natural ontogenesis of Capsicum annuum leaves, by accelerating and enhancing the typical characteristics of ageing, and, additionally, it changed the structure and physiology of cells.
    Źródło:
    Acta Societatis Botanicorum Poloniae; 2003, 72, 3
    0001-6977
    2083-9480
    Pojawia się w:
    Acta Societatis Botanicorum Poloniae
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Tapetum development in transgenic tobacco [Nicotiana tabacum L.] plants with modified level of histone H1 variants
    Autorzy:
    Slusarczyk, J
    Tykarska, T.
    Wierzbicki, A.
    Jerzmanowski, A.
    Kuras, M.
    Powiązania:
    https://bibliotekanauki.pl/articles/58139.pdf
    Data publikacji:
    2006
    Wydawca:
    Polskie Towarzystwo Botaniczne
    Tematy:
    tapetum
    development
    transgenic plant
    tobacco
    Nicotiana tabacum
    histone level
    H1 variant
    sterile pollen grain
    Opis:
    The phenomenon of male sterility has often been observed in investigations on the role of histone H1 in regulation of morphogenetic and cytological processes in transgenic tobacco plants. These changes were accumulated by disturbances in flower development, consisting in lengthening of the pistil style in relation to stamen heads. This prevented pollination and production of seeds. As similar abnormalities occurred also in the present investigations (depending on combination, the sterility% was 84.4 to 19.9, at only 8.1 in the control), the main problem of our investigations was an attempt to explain their reasons. It is commonly known that one of the conditions for formation of fertile pollen is the properly functioning tapetum. Here, we carried out observations of ultrastructure of anther tapetum control cells in respect of abnormalities which occurred during microsporogenesis of transgenic plants with inactivated expression of two major (A, B) and two minor (C, D) histone H1 variants. The investigations were carried out on the following groups of plants: (1) control group with a full set of histone variants (K), (2) with inactivated A and B variants (-AB); (3) with inactivated A, B, C and D variants (-ABCD), (4) with inactivated C and D variants (-CD). It was found that tapetal development was normal in all the investigated groups of plants, and the sequence of changes was similar as in the control. However, certain ultrastructural differences appeared when tapetum functioned as secretory tissue, and in the degeneration phase. In tapetal cell cytoplasm, with participation of rER, lipid bodies were formed, which, having penetrated to the cell surface and to locules, took part in formation of pollen grain sporoderm. Both in the control and in the remaining combination, excluding -ABCD, these bodies looked similar: they were grey, homogenous and surrounded by black jagged deposits. In -ABCD plants, these bodies were more translucent, slightly rarefied, and not surrounded by the deposits. Moreover, in -CD plants, large lipid deposits were frequently observed between remainders of degraded tapetal cells. They did not occur in the control and the remaining combinations.
    Źródło:
    Acta Societatis Botanicorum Poloniae; 2006, 75, 2; 95-105
    0001-6977
    2083-9480
    Pojawia się w:
    Acta Societatis Botanicorum Poloniae
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
      Wyświetlanie 1-3 z 3

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