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    Wyświetlanie 1-4 z 4
    Tytuł:
    Evaluation of hydrophobicity and quantitative analysis of biofilm formation by Alicyclobacillus sp.
    Autorzy:
    Tyfa, Agnieszka
    Kunicka-Styczyńska, Alina
    Zabielska, Julia
    Powiązania:
    https://bibliotekanauki.pl/articles/1038914.pdf
    Data publikacji:
    2015
    Wydawca:
    Polskie Towarzystwo Biochemiczne
    Tematy:
    Alicyclobacillus sp.
    acidothermophilic bacteria
    biofilm
    hydrophobicity
    glass surface
    Opis:
    Alicyclobacillus sp. are acidothermophilic bacteria frequently contaminating fruit based products (juices and juice concentrates). These sporulating bacteria are able to survive at elevated temperatures and highly acidic environments which causes difficulties in their removal from industrial environments. Although numerous literature data examine Alicyclobacillus sp. presence in fruit based products and methods of their elimination, there is still a limited knowledge on ability of these bacteria to adhere to abiotic surfaces. Therefore, the objective of this study was to determine Alicyclobacillus sp. cells' hydrophobicity and capability of biofilm formation on a glass surface. The degree of cells hydrophobicity, according to Microbial Adhesion to Hydrocarbon (MATH) and Salt Aggregation Test (SAT), was investigated for eleven environmental isolates from natural Polish habitats, identified as Alicyclobacillus sp., and a Alicyclobacillus acidoterrestris DSM 3922 reference strain. The dynamics of biofilm formation within 3-day incubation on a glass surface was evaluated and quantified by a plate count method both, for cultures with and without agitation. All of the bacterial strains tested expressed ability to colonize a glass surface and four environmental isolates were classified as fast-adherent strains. The mature biofilm structures were predominantly formed after 48 hours of incubation. Dynamic culturing conditions were observed to accelerate the biofilm formation. The majority of strains expressed a moderate hydrophobicity level both, in SAT (41.7%) and MATH-PBS (75.0%), as well as MATH-PUM (91.7%) tests. However, no correlation between hydrophobicity and cell adherence to a glass slide surface was observed.
    Źródło:
    Acta Biochimica Polonica; 2015, 62, 4; 785-790
    0001-527X
    Pojawia się w:
    Acta Biochimica Polonica
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Experimental investigations of the PMMA bone cement distribution inside a model of lumbar vertebrae
    Autorzy:
    Bonik, Artur
    Tyfa, Zbigniew
    Ciupik, Lechosław Franciszek
    Sobczak, Krzysztof
    Kierzkowska, Agnieszka
    Obidowski, Damian Stanisław
    Witkowski, Dariusz
    Pałczyński, Tomasz
    Elgalal, Marcin
    Komorowski, Piotr
    Słoński, Edward
    Jóźwik, Krzysztof
    Walkowiak, Bogdan
    Powiązania:
    https://bibliotekanauki.pl/articles/2232172.pdf
    Data publikacji:
    2022
    Wydawca:
    Akademia Górniczo-Hutnicza im. Stanisława Staszica w Krakowie. Polskie Towarzystwo Biominerałów
    Tematy:
    percutaneous vertebroplasty
    osteoporotic spinal fractures
    spinal cement injection
    cement distribution
    bone cement preparation
    Opis:
    The use of bone cement in procedures such as vertebroplasty and kyphoplasty can reduce pain and mechanically support the spine. This study aimed to evaluate whether air entrapped within bone cement affected its distribution in a vertebral body model. The study included 3D printed anatomical models of vertebrae together with their internal trabecular structure. Aeration was achieved by mixing the bone cement using three different altered procedures, whilst the control sample was prepared according to the manufacturer’s instructions. The further two samples were prepared by reducing or increasing the number of cycles required to mix the bone cement. A test rig was used to administer the prepared bone cement and introduce it into the vertebral model. Each time the injection was stopped when either the cement started to flow out of the vertebral model or when the entire cement volume was administered. The computer tomography (CT) scanning was performed to evaluate aerification and its influence on the bone cement distribution in each of the patient-specific models. The large number of small pores visible within the cement, especially in the cannula vicinity, indicated that the cement should not be treated as a homogenous liquid. These results suggest that a high level of aerification can influence the further cement distribution.
    Źródło:
    Engineering of Biomaterials; 2022, 25, 165; 23--30
    1429-7248
    Pojawia się w:
    Engineering of Biomaterials
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Methods for eradication of the biofilms formed by opportunistic pathogens using novel techniques – A review
    Autorzy:
    Zabielska, Julia
    Tyfa, Agnieszka
    Kunicka-Styczyńska, Alina
    Powiązania:
    https://bibliotekanauki.pl/articles/764814.pdf
    Data publikacji:
    2016
    Wydawca:
    Uniwersytet Łódzki. Wydawnictwo Uniwersytetu Łódzkiego
    Tematy:
    biofilm eradication
    Pseudomonas aeruginosa
    microbial colonization
    Opis:
    W niekorzystnych warunkach środowiska, mikroorganizmy zasiedlają zarówno powierzchnie abiotyczne, jak i biotyczne takie jak tkanki zwierzęce czy roślinne, tworząc struktury biofilmu charakteryzujące się wysoką opornością. Adhezja mikroorganizmów, szczególnie patogenów oportunistycznych, niesie niebezpieczeństwo zasiedlania materiałów medycznych, co może doprowadzić do infekcji u osób z obniżoną odpornością. Chociaż dotychczasowe badania wskazują różne metody zapobiegania tworzeniu biofilmu, jego całkowita eliminacja ze środowiska jest nadal niemożliwa. Przedstawione opracowanie stanowi przegląd nowoczesnych metod usuwania dojrzałego biofilmu tworzonego przez patogeny oportunistyczne. Spośród wielu metod opisano m.in. zastosowanie: zimnej plazmy, ultradźwięków, pola elektrycznego, ozonowania wody, terapii fagowej, enzymów działających bezpośrednio na macierz biofilmu, bakteriocyn, środków chemicznych syntetycznych oraz pochodzenia naturalnego.
    The inconvenient environmental conditions force microorganisms to colonize either abiotic surfaces or animal and plant tissues and, therefore, form more resistant structures – biofilms. The phenomenon of microbial adherence, opportunistic pathogens in particular, is of a great concern. Colonization of medical devices and biofilm formation on their surface, may lead to severe infections mainly in humans with impaired immune system. Although, current research consider various methods for prevention of microbial biofilms formation, still, once a biofilm is formed, its elimination is almost impossible. This study focuses on the overview of novel methods applied for eradication of mature opportunistic pathogens' biofilms. Among various techniques the following: cold plasma, electric field, ultrasounds, ozonated water treatment, phagotherapy, matrix targeting enzymes, bacteriocins, synthetic chemicals and natural origin compounds used for biofilm matrix disruption were briefly described.
    Źródło:
    Acta Universitatis Lodziensis. Folia Biologica et Oecologica; 2016, 12; 26-37
    1730-2366
    2083-8484
    Pojawia się w:
    Acta Universitatis Lodziensis. Folia Biologica et Oecologica
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Opportunistic Gram-negative rods capability of creating biofilm structures on polivynyl chloride and styrene-acronitrile copolymer surfaces
    Autorzy:
    Zabielska, Julia
    Kunicka-Styczyńska, Alina
    Rajkowska, Katarzyna
    Tyfa, Agnieszka
    Powiązania:
    https://bibliotekanauki.pl/articles/1038901.pdf
    Data publikacji:
    2015
    Wydawca:
    Polskie Towarzystwo Biochemiczne
    Tematy:
    Pseudomonas aeruginosa
    biofilm
    environmental strains
    polymer surfaces
    Opis:
    Biofilms are highly organized microbial communities displaying high resistance to disinfectants and other external environmental factors. Medical equipment, such as stents and catheters, can be colonized by a variety of bacteria including opportunistic pathogens circulating in the environment and dangerous to immunocompromised patients. Application of materials resistant to biofilm formation will minimize the risk of patients' infection. Hence, the aim of this research was to determine the biofilm growth of environmental bacteria isolates on polyvinyl chloride and styrene-acronitrile copolymer surfaces. Nine strains (Pseudomonas aeruginosa, Burkholderia cepacia and Serratia liquefacies) isolated from cosmetics, and a reference P. aeruginosa strain ATCC 15442, were tested. The ability and dynamics of biofilm formation on intubation catheters (30°C, up to 24 h) in bacterial growth cultures (107-108 CFU/ml) was investigated, with subsequent sonication and quantification by agar plate count method. The results indicated that all the tested bacteria expressed a strong ability for the polymer surface adhesion, reaching 4.6 to 6.7 log CFU/cm2 after 30 minutes. Moreover, for the majority of strains, the level of 24-hour biofilm production was from 6.67-7.61 log CFU/cm2. This research indicates that the environmental strains circulating between the cosmetics and patients may pose a threat of biofilm formation on medical equipment surfaces, and presumably in the clinical surroundings as well.
    Źródło:
    Acta Biochimica Polonica; 2015, 62, 4; 733-737
    0001-527X
    Pojawia się w:
    Acta Biochimica Polonica
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
      Wyświetlanie 1-4 z 4

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