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    Wyświetlanie 1-2 z 2
    Tytuł:
    Improving growth indices and productivity of phytochemical compounds in lemon balm (Melissa officinalis L.) through induced polyploidy
    Autorzy:
    Talei, D.
    Fotokian, M.H.
    Powiązania:
    https://bibliotekanauki.pl/articles/2097129.pdf
    Data publikacji:
    2020
    Wydawca:
    Polska Akademia Nauk. Czytelnia Czasopism PAN
    Tematy:
    colchicine
    flavonoid
    lemon balm
    physiological traits
    rosmarinic acid
    Opis:
    The induction of polyploidy using mutagenic chemicals is one of the plant breeding methods to enhance the production of secondary metabolites. In the current research, to induce polyploidy in lemon balm (Melissa officinalis) plants, seeds were treated with different concentrations of colchicine for various exposure times. A factorial experiment was performed using a randomized complete block design with two factors: colchicine concentrations with four levels (control, 0.05%, 0.1%, and 0.2%) and exposure times with three levels (24, 48, and 72 h) and three replicates. The physiological and phytochemical traits of plants were measured at a 4–6 leave stage. The results indicated that different concentrations of colchicine had a significant effect on the chlorophyll a (Chl.a), chlorophyll b (Chl.b), carotenoid, phenol, flavonoid, and rosmarinic acid contents. The exposure times of colchicine also caused significant changes in Chl.a, Chl.b, carotenoid, phenol, flavonoid, and rosmarinic acid amounts (P # 0.01). Increasing the colchicine concentration significantly increased the physiological and phytochemical traits at 0.05% and 0.1% concentration in comparison to the control (P # 0.01). In contrast, the interaction of colchicine concentration and exposure time had a significant effect on Chl.a, Chl.b, carotenoid, and rosmarinic acid amounts. The findings of this study indicate that one of the effective methods in primary screening of polyploidy plants in the polyploidization breeding program is the estimation of the physiological changes, the contents of chlorophyll a and b, and the total amount of chlorophyll and secondary metabolites. Flow cytometry is recommended to be used for the accurate identification of the ploidy level in M. officinalis.
    Źródło:
    BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology; 2020, 101, 3; 215-226
    0860-7796
    Pojawia się w:
    BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Protein patterns and their association with photosynthetic pigment content, agronomic behavior, and origin of purslane accessions (Portulaca oleracea L.)
    Autorzy:
    Talei, D.
    Naji, A.M.
    Powiązania:
    https://bibliotekanauki.pl/articles/2096425.pdf
    Data publikacji:
    2021
    Wydawca:
    Polska Akademia Nauk. Czytelnia Czasopism PAN
    Tematy:
    chlorophyll
    genetic diversity
    morphometric
    Portulaca oleracea
    protein pattern
    Opis:
    In this study, the proteomic, morphometric, and photosynthetic pigment data of purslane (Portulaca oleracea) accessions were combined together to show their impact on genetic variation in order to establish a relationship between protein patterns and phenotypic behavior of the plant. Seeds of 18 collected purslane accessions were cultivated based on a completely randomized design with three replicates. Before the flowering stage, the data on morphology, photosynthetic pigment content, and seed proteins were obtained. The results showed a significant difference among purslane accessions in terms of the most studied agronomic characteristics and the content of photosynthetic pigments and proteins. The cluster analysis of the 18 purslane accessions based on agronomic data, and photosynthetic pigment content, and protein pattern data produced three main clusters. Moreover, the seed protein analysis revealed that the two polymorphic protein bands of size 40 kDa (protein “a”) and 30 kDa (protein “b”) effectively diversified the agronomic, photosynthetic pigment, and phylogenetic relationships among the purslane accessions. Interestingly, protein “a” was produced in plants growing in low altitude areas and played a suppressive role for TDW, while protein “b” was produced in plants growing in high altitude areas and functioned as an activator agent for this trait. Overall, the outcomes of the present study indicated the presence of high genetic variability (77.6%) among the purslane accessions. These findings suggest that these proteins should be sequenced for further proteomic analyses and can be used for hybridization to generate useful recombinants in segregating generations and improve breeding varieties of P. oleracea.
    Źródło:
    BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology; 2021, 102, 3; 245-255
    0860-7796
    Pojawia się w:
    BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
      Wyświetlanie 1-2 z 2

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