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Wyszukujesz frazę "Szmidt-Jaworska, A." wg kryterium: Autor

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    Wyświetlanie 1-4 z 4
    Tytuł:
    Molecular cloning, characterization and expression of a calcium dependent protein kinase (CDPK) involved in the stress signalling in Hippeastrum x hybr.
    Autorzy:
    Szewczuk, P.
    Swiezawska, B.
    Pawelek, A.
    Jaworski, K.
    Szmidt-Jaworska, A.
    Powiązania:
    https://bibliotekanauki.pl/articles/80573.pdf
    Data publikacji:
    2013
    Wydawca:
    Polska Akademia Nauk. Czytelnia Czasopism PAN
    Tematy:
    conference
    molecular cloning
    expression
    calcium-dependent protein kinase
    flowering plant
    Hippeastrum x hybridum
    biotic stress
    abiotic stress
    Źródło:
    BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology; 2013, 94, 3
    0860-7796
    Pojawia się w:
    BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Optimization of extraction and purification of RNA from plant bulbs
    Autorzy:
    Duszyn, M.
    Swiezawska, B.
    Kwiatkowski, M.
    Jaworski, K.
    Szmidt-Jaworska, A.
    Powiązania:
    https://bibliotekanauki.pl/articles/2117778.pdf
    Data publikacji:
    2021
    Wydawca:
    Polska Akademia Nauk. Czasopisma i Monografie PAN
    Tematy:
    bulbs
    ornamental plants
    polyphenols
    polysaccharides
    RNA isolation
    Opis:
    RNA extraction involves several main stages, regardless of the method of extraction: homogenization, effective denaturation of proteins from RNA, inactivation of ribonuclease and removal of any DNA, protein, and some residual contamination. Isolation of undamaged intact RNA is challenging when the related tissue contains high levels of polysaccharides and phenols. Several efforts have been made towards the comparison and optimization of extraction and purification methods for RNA from plant tissues. This is dictated by the necessity of obtaining RNA of a good quality and in a sufficient quantity for further molecular analyzes. Plant storage organs (such as bulbs or seeds) rich in polysaccharide and polyphenolic compounds present distinct challenges for total RNA isolation. Such components, considered in this case as contamination, may bind and co-precipitate with nucleic acids and negatively affect later assays. Since standard routine protocols yield unacceptable results in bulbs, we have designed a new method for RNA extraction. We used two modified procedures (based on CTAB and sarkosyl reagents) of RNA extraction from so called “difficult plant material” and compared them to a popular RNA isolation base on the column isolation kit and TriPure reagent. Our modified protocols dealt with problems of both RNA degradation and low yield caused by co-purification with polysaccharides present in plant bulbs. In this study we have shown that improvement of the CTAB and sarkosyl method with a lyophilization step of plant tissues leads to isolation of high quality RNA from difficult material like storage organs of bulbous plants. The main changes in the procedure compared to the previously described methods concerned the different order of lithium chloride and sodium acetate addition, lithium chloride concentration increase and modification of centrifugation conditions. Gel electrophoresis and spectrophotometer analysis confirmed the high quality and integrity of the obtained RNA. The modified procedures allowed for obtaining a satisfying amount of RNA concentration in the range from 280 to 950 ng/μl depending on the plant species. Thus, the demonstrated RNA isolation methods are efficient and can be used for plant material rich in polysaccharides, such as bulbs.
    Źródło:
    Acta Biologica Cracoviensia. Series Botanica; 2020, 62, 2; 17-30
    0001-5296
    Pojawia się w:
    Acta Biologica Cracoviensia. Series Botanica
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Interactive physiological response of potato (Solanum tuberosum L.) plants to fungal colonization and Potato virus Y (PVY) infection
    Autorzy:
    Thiem, D.
    Szmidt-Jaworska, A.
    Baum, C.
    Muders, K.
    Niedojadlo, K.
    Hrynkiewicz, K.
    Powiązania:
    https://bibliotekanauki.pl/articles/67360.pdf
    Data publikacji:
    2014
    Wydawca:
    Polskie Towarzystwo Botaniczne
    Opis:
    Potato plants can be colonized by various viruses and by symbiotic, saprophytic and pathogenic fungi. However, the significance of interactions of viral infection and fungal colonization is hardly known. This work presents a model experiment in which the influence of three different types of fungal associations on the growth and physiology of the potato variety Pirol was tested individually or in combination with infection by PVY. It was hypothesized that simultaneous viral and fungal infections increase the biotic stress of the host plant, but mutualistic plant-fungal associations can mask the impact of viral infection. In the present study, a symbiotic arbsucular mycorrhizal fungus, Glomus intraradices, significantly stimulated the growth of plants infected with PVY. In contrast, two saprophytic Trichoderma spp. strains either did not influence or even inhibited the growth of PVY-infected plants. Also, inoculation of PVY-infected potato plants with a pathogenic strain of Colletotrichum coccodes did not inhibit the plant growth. Growth of the PVY-free potato plants was not promoted by the symbiotic fungus, whereas T. viride, T. harzianum and C. coccodes had an evident inhibitory effect. The strongest growth inhibition and highest concentration of H2O2, as an indicator of biotic stress, was observed in PVY-free potato plants inoculated with T. harzianum and C. coccodes strains. Surprisingly, ultrastructural analysis of PVY-infected plant roots colonized by G. intraradices showed virus-like structures in the arbuscules. This pointed to the possibility of mycorrhizal-mediated transmission of virus particles and has to be further biotic stress; Potato virus Y (PVY); Glomus intraradices; Trichoderma viride; T. harzianum; Colletotrichum coccodesexamined by testing with immunoassays and real transmission to uninfected plants. In conclusion, although mycorrhiza formation might decrease the impact of PVY infection on plants, a possible role of mycorrhizal fungi as virus vectors is discussed.
    Źródło:
    Acta Mycologica; 2014, 49, 2
    0001-625X
    2353-074X
    Pojawia się w:
    Acta Mycologica
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    The role of jasmonates in the formation of a compound of chalcones and flavans with phytoalexin-like properties in mechanically wounded scales of Hippeastrum × hybr. bulbs
    Autorzy:
    Wilmowicz, E.
    Frankowski, K.
    Grzegorzewska, W.
    Kesy, J.
    Kucko, A.
    Banach, M.
    Szmidt-Jaworska, A.
    Saniewski, M.
    Powiązania:
    https://bibliotekanauki.pl/articles/19110.pdf
    Data publikacji:
    2014
    Wydawca:
    Polska Akademia Nauk. Czytelnia Czasopism PAN
    Źródło:
    Acta Biologica Cracoviensia. Series Botanica; 2014, 56, 1
    0001-5296
    Pojawia się w:
    Acta Biologica Cracoviensia. Series Botanica
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
      Wyświetlanie 1-4 z 4

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