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    Wyświetlanie 1-15 z 15
    Tytuł:
    The construction of the hybrid plasmid containing genes of the central part of phage T4 baseplate and gene 29 product separation
    Autorzy:
    Nieradko, Józef
    Szewczyk, Bogusław
    Powiązania:
    https://bibliotekanauki.pl/articles/1045868.pdf
    Data publikacji:
    1988
    Wydawca:
    Polskie Towarzystwo Biochemiczne
    Źródło:
    Acta Biochimica Polonica; 1988, 35, 4; 357-366
    0001-527X
    Pojawia się w:
    Acta Biochimica Polonica
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Antivirals - current trends in fighting influenza
    Autorzy:
    Król, Ewelina
    Rychłowska, Małgorzata
    Szewczyk, Bogusław
    Powiązania:
    https://bibliotekanauki.pl/articles/1039254.pdf
    Data publikacji:
    2014
    Wydawca:
    Polskie Towarzystwo Biochemiczne
    Tematy:
    influenza virus
    antivirals
    novel anti-influenza drugs
    antiviral therapy
    inhibitors
    Opis:
    Influenza virus infection is a major source of morbidity and mortality worldwide. Due to the variable effectiveness of existing vaccines, especially in the early stages of an epidemic, antiviral drugs represent the first line of defense against the virus. Currently, there are two major classes of anti-influenza drugs approved by the FDA for clinical use: M2 protein inhibitors (amantadine and rimantadine) and neuraminidase inhibitors (zanamivir and oseltamivir). However, increasing resistance to these available influenza antivirals among circulating influenza viruses highlights the need to develop alternative approaches for the prevention and/or treatment of influenza. This review presents an overview of currently available drugs for influenza treatment as well as summarizes some new antiviral strategies that are now being tested covering agents targeting both the viral proteins and the host-virus interaction. We discuss their mechanisms of action, resistance and the therapeutic potential as new antiviral drug for use in future influenza pandemics. Additionally, combination therapy based on these drugs is also described.
    Źródło:
    Acta Biochimica Polonica; 2014, 61, 3; 495-504
    0001-527X
    Pojawia się w:
    Acta Biochimica Polonica
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Effect of N-glycosylation inhibition on the synthesis and processing of classical swine fever virus glycoproteins
    Autorzy:
    Tyborowska, Jolanta
    Zdunek, Ewa
    Szewczyk, Bogusław
    Powiązania:
    https://bibliotekanauki.pl/articles/1040876.pdf
    Data publikacji:
    2007
    Wydawca:
    Polskie Towarzystwo Biochemiczne
    Tematy:
    glycoproteins
    pestivirus
    glycosylation
    classical swine fever virus
    Opis:
    Classical swine fever virus (CSFV) is often used as a surrogate model in molecular studies of the closely related hepatitis C virus. In this report we have examined the effect of the inhibition of glycosylation on the survival and maturation of CSFV. Viral glycoproteins (Erns, E1, E2) form biologically active complexes - homo- and heterodimers, which are indispensable for viral life cycle. Those complexes are highly N-glycosylated. We studied the influence of N-glycosylation on dimer formation using Erns and E2 glycoproteins produced in insect cells after infection with recombinant baculoviruses. The glycoproteins were efficiently synthesized in insect cells, had similar molecular masses and formed dimers like their natural counterparts. Surprisingly, the addition of tunicamycin (an antibiotic which blocks early steps of glycosylation) to insect cell culture blocked not only dimer formation but it also led to an almost complete disappearance of E2 even in monomeric form. Tunicamycin did not exert a similar effect on the synthesis and formation of Erns dimers; the dimers were still formed, which suggests that Erns glycan chains are not necessary for dimer formation. We have also found that very low doses of tunicamycin (much lower than those used for blocking N-glycosylation) drastically reduced CSFV spread in SK6 (swine kidney) cell culture and the virus yield. These facts indicate that N-glycosylation inhibitors structurally similar to tunicamycin may be potential therapeutics for the inhibition of the spread of CSFV and related viruses.
    Źródło:
    Acta Biochimica Polonica; 2007, 54, 4; 813-819
    0001-527X
    Pojawia się w:
    Acta Biochimica Polonica
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Introduction to molecular biology of influenza a viruses
    Autorzy:
    Szewczyk, Bogusław
    Bieńkowska-Szewczyk, Krystyna
    Król, Ewelina
    Powiązania:
    https://bibliotekanauki.pl/articles/1039236.pdf
    Data publikacji:
    2014
    Wydawca:
    Polskie Towarzystwo Biochemiczne
    Tematy:
    Orthomyxoviridae
    influenza A virus
    virion structure
    genome
    entry
    release
    new polypeptides
    Opis:
    This minireview presents an overview of current knowledge on virion structure, genome organization and basic events in the development of influenza A virus. The processes of entry, transcription/replication and viral release are described. In this context, the roles of viral proteins (including recently discovered minor polypeptides) in the subsequent stages of viral development are also discussed.
    Źródło:
    Acta Biochimica Polonica; 2014, 61, 3; 397-401
    0001-527X
    Pojawia się w:
    Acta Biochimica Polonica
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Effect of tunicamycin on the biogenesis of hepatitis C virus glycoproteins
    Autorzy:
    Reszka, Natalia
    Krol, Ewelina
    Patel, Arvind
    Szewczyk, Boguslaw
    Powiązania:
    https://bibliotekanauki.pl/articles/1040320.pdf
    Data publikacji:
    2010
    Wydawca:
    Polskie Towarzystwo Biochemiczne
    Tematy:
    glycoproteins
    glycosylation inhibition
    hepatitis C virus
    tunicamycin
    Opis:
    Hepatitis C virus (HCV) infects humans, with a prevalence around 3% of population, causing acute and chronic hepatitis and hepatocellular carcinoma. We studied the effect of inhibition of glycosylation on the assembly of the HCV particle. HCV possesses two envelope glycoproteins E1 and E2 that are highly modified by N-glycans. These glycan residues are crucial for viral entry and maturation of the progeny. Here, we examined the influence of inhibition of N-glycosylation on expression of E1 and E2. Since the propagation of HCV in cell culture is limited, we used a recombinant baculovirus producing viral-like particles in insect cells. Our data showed that blocking of N-glycan transfer to the nascent polypeptide chain with the antibiotic tunicamycin resulted in the loss of E1 and E2. We also found that a dose of tunicamycin that did not influence the cell viability significantly reduced the E2 level in infected cells. The results indicate that blocking of glycosylation at an early step efficiently reduces the assembly of HCV virions. Thus, we suggest that derivatives of tunicamycin that preferentially block glycosylation of viral proteins may become potential therapeutic agents against HCV.
    Źródło:
    Acta Biochimica Polonica; 2010, 57, 4; 541-546
    0001-527X
    Pojawia się w:
    Acta Biochimica Polonica
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Expression of bacteriophage T4 minor baseplate proteins in the bacteriophage T7 promoter/RNA polymerase expression system
    Autorzy:
    Schlichtholtz, Beata
    Nieradko, Józef
    Gajek, Małgorzata
    Szewczyk, Bogusław
    Powiązania:
    https://bibliotekanauki.pl/articles/1045704.pdf
    Data publikacji:
    1991
    Wydawca:
    Polskie Towarzystwo Biochemiczne
    Źródło:
    Acta Biochimica Polonica; 1991, 38, 3; 311-319
    0001-527X
    Pojawia się w:
    Acta Biochimica Polonica
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Hemagglutinin stalk domain from H5N1 strain as a potentially universal antigen
    Autorzy:
    Uranowska, Karolina
    Tyborowska, Jolanta
    Jurek, Anna
    Szewczyk, Bogusław
    Gromadzka, Beata
    Powiązania:
    https://bibliotekanauki.pl/articles/1039261.pdf
    Data publikacji:
    2014
    Wydawca:
    Polskie Towarzystwo Biochemiczne
    Tematy:
    H5N1
    universal influenza antigen
    hemagglutinin stalk domain
    universal influenza antibodies
    FI6
    Opis:
    Influenza A virus infections are the major public health concern and cause significant morbidity and mortality each year worldwide. Vaccination is the main strategy of influenza epidemic prevention. However, seasonal vaccines induce strain-specific immunity and must be reformulated annually based on prediction of the strains that will circulate in the next season. Thus, it is essential to develop vaccines that would induce broad and persistent immunity to influenza viruses. Hemagglutinin is the major surface antigen of the influenza virus. Recent studies revealed the importance of HA stalk-specific antibodies in neutralization of different influenza virus strains. Therefore, it is important to design an immunogen that would focus the immune response on the HA stalk domain in order to elicit neutralizing antibodies. In the present study, we report characterization of a conserved truncated protein, potentially a universal influenza virus antigen from the H5N1 Highly Pathogenic Avian Influenza A virus strain. Our results indicate that exposure of the HA stalk domain containing conserved epitopes results in cross reactivity with different antibodies (against group 1 and 2 HAs). Additionally, we conclude that HA stalk domain contains not only conformational epitopes recognized by universal FI6 antibody, but also linear epitopes recognized by other antibodies.
    Źródło:
    Acta Biochimica Polonica; 2014, 61, 3; 541-550
    0001-527X
    Pojawia się w:
    Acta Biochimica Polonica
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Detection and differentiation of Newcastle disease virus and influenza virus by using duplex real-time PCR
    Autorzy:
    Nidzworski, Dawid
    Wasilewska, Edyta
    Smietanka, Krzysztof
    Szewczyk, Bogusław
    Minta, Zenon
    Powiązania:
    https://bibliotekanauki.pl/articles/1039554.pdf
    Data publikacji:
    2013
    Wydawca:
    Polskie Towarzystwo Biochemiczne
    Tematy:
    Newcastle disease virus
    influenza virus
    detection
    differentiation
    real-time PCR
    duplex
    Opis:
    Newcastle disease virus (NDV), member of the Paramyxoviridae family and avian influenza virus (AIV), member of the Orthomyxoviridae family, are two main avian pathogens causing serious economic problems in poultry health. Both are enveloped, single-stranded, negative-sense RNA viruses and cause similar symptoms, ranging from sub-clinical infections to severe diseases, including decrease in egg production, acute respiratory syndrome, and high mortality. Similar symptoms hinder the differentiation of infection with the two viruses by standard veterinary procedures like clinical examination or necropsy. To overcome this problem, we have developed a new duplex real-time PCR assay for the detection and differentiation of these two viruses. Eighteen NDV strains, fourteen AIV strains, and twelve other (negative control) strains viruses were isolated from allantoic fluids of specific pathogen-free (SPF), embryonated eggs. Four-weeks-old SPF chickens were co-infected with both viruses (NDV - LaSota and AIV - H7N1). Swabs from cloaca and trachea were collected and examined. The results obtained in this study show that by using duplex real-time PCR, it was possible to detect and distinguish both viruses within less than three hours and with high sensitivity, even in case a bird was co-infected. Additionally, the results show the applicability of the real-time PCR assay in laboratory practice for the identification and differentiation of Newcastle disease and influenza A viruses in birds.
    Źródło:
    Acta Biochimica Polonica; 2013, 60, 3; 475-480
    0001-527X
    Pojawia się w:
    Acta Biochimica Polonica
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Recombinant VP60 in the form of virion-like particles as a potential vaccine against rabbit hemorrhagic disease virus*
    Autorzy:
    Gromadzka, Beata
    Szewczyk, Bogusław
    Konopa, Grażyna
    Fitzner, Andrzej
    Kęsy, Andrzej
    Powiązania:
    https://bibliotekanauki.pl/articles/1041251.pdf
    Data publikacji:
    2006
    Wydawca:
    Polskie Towarzystwo Biochemiczne
    Tematy:
    baculovirus
    VLP
    RHDV
    VP60
    Opis:
    Rabbit hemorrhagic disease virus (RHDV) which causes a highly contagious disease of wild and domestic rabbits belongs to the family Caliciviridae. It is a small, positive single-stranded RNA virus with a genome of 7.5 kb and has a diameter of approximately 40 nm. In negatively stained electron micrographs the virus shows typical calicivirus morphology with regularly arranged cup-shaped structures on the surface. It is a major pathogen of rabbits in many countries. Vp60 - a coat protein of molecular mass around 60 kDa is the major antigen of RHDV. It is present as 90 dimeric units per virion particle. We have expressed VP60 gene in the baculovirus system with the aim to use it as a potential vaccine against RHDV and a diagnostic reagent in immunological tests. cDNA of the vp60 gene of strain SGM, was cloned into a baculovirus transfer vector as full-length gene, as well as truncated gene lacking 600 5´-terminal nucleotides. The sequence of SGM VP60 differed markedly from that of the reference strain. Full-length recombinant VP60 protein from the SGM strain self-assembled to form virus-like particles (VLPs). These particles observed by electron microscopy were morphologically similar to native virions and were able to agglutinate human group 0 erythrocytes. After immunization the recombinant particles induced RHDV-specific antibodies in rabbits and guinea pigs. Rabbits immunized with the VLPs were fully protected against challenge with a virulent RHDV.
    Źródło:
    Acta Biochimica Polonica; 2006, 53, 2; 371-376
    0001-527X
    Pojawia się w:
    Acta Biochimica Polonica
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Characterization of mAb6-9-1 monoclonal antibody against hemagglutinin of avian influenza virus H5N1 and its engineered derivative, single-chain variable fragment antibody
    Autorzy:
    Sawicka, Róża
    Siedlecki, Paweł
    Kalenik, Barbara
    Radomski, Jan
    Sączyńska, Violetta
    Porębska, Anna
    Szewczyk, Bogusław
    Sirko, Agnieszka
    Góra-Sochacka, Anna
    Powiązania:
    https://bibliotekanauki.pl/articles/1038689.pdf
    Data publikacji:
    2017
    Wydawca:
    Polskie Towarzystwo Biochemiczne
    Tematy:
    influenza virus
    monoclonal antibody
    scFv
    hemagglutinin
    antigenic epitope
    Opis:
    Hemagglutinin (HA), as a major surface antigen of influenza virus, is widely used as a target for production of neutralizing antibodies. Monoclonal antibody, mAb6-9-1, directed against HA of highly pathogenic avian influenza virus A/swan/Poland/305-135V08/2006(H5N1) was purified from mouse hybridoma cells culture and characterized. The antigenic specificity of mAb6-9-1 was verified by testing its cross-reactivity with several variants of HA. The mimotopes recognized by mAb6-9-1 were selected from two types of phage display peptide libraries. The comparative structural model of the HA variant used for antibody generation was developed to further facilitate epitope mapping. Based on the sequences of the affinity- selected polypeptides and the structural model of HA the epitope was located to the region near the receptor binding site (RBS). Such localization of the epitope recognized by mAb6-9-1 is in concordance with its moderate hemagglutination inhibiting activity and its antigenic specificity. Additionally, total RNA isolated from the hybridoma cell line secreting mAb6-9-1 was used for obtaining two variants of cDNA encoding recombinant single-chain variable fragment (scFv) antibody. To ensure high production level and solubility in bacterial expression system, the scFv fragments were produced as chimeric proteins in fusion with thioredoxin or displayed on a phage surface after cloning into the phagemid vector. Specificity and affinity of the recombinant soluble and phage-bound scFv were assayed by suitable variants of ELISA test. The observed differences in specificity were discussed.
    Źródło:
    Acta Biochimica Polonica; 2017, 64, 1; 85-92
    0001-527X
    Pojawia się w:
    Acta Biochimica Polonica
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Expression of avian influenza haemagglutinin (H5) and chicken interleukin 2 (chIL-2) under control of the ptcB promoter in Lactococcus lactis
    Autorzy:
    Szatraj, Katarzyna
    Szczepankowska, Agnieszka
    Sączyńska, Violetta
    Florys, Katarzyna
    Gromadzka, Beata
    Łepek, Krzysztof
    Płucienniczak, Grażyna
    Szewczyk, Bogusław
    Zagórski-Ostoja, Włodzimierz
    Bardowski, Jacek
    Powiązania:
    https://bibliotekanauki.pl/articles/1039270.pdf
    Data publikacji:
    2014
    Wydawca:
    Polskie Towarzystwo Biochemiczne
    Tematy:
    Lactococcus lactis
    ptcB promoter
    heterologous gene expression
    avian influenza H5N1
    H5 haemagglutinin
    chicken interleukin-2
    Opis:
    Gram-positive and nonpathogenic lactic acid bacteria (LAB) are considered to be promising candidates for the development of new, safe systems of heterologous protein expression. Recombinant LAB has been shown to induce specific local and systemic immune response against selected pathogens, and could be a good alternative to classical attenuated carriers. The main goal of our study was to express the avian influenza haemagglutinin (H5) and chicken interleukin 2 (chIL-2) in Lactococcus lactis. Results of this study were anticipated to lead to construction of lactococcal strain(s) with potential vaccine properties against the avian influenza A (H5N1) virus. Expression of the cloned H5 gene, its His-tagged variant and chIL-2 gene, under the control of the ptcB gene promoter was attested by RT-PCR on transcriptional level and Western or dot blot analysis on translational level, demonstrating that system can be an attractive solution for production of heterologous proteins. The results of the preliminary animal trial conducted in mice are a promising step toward development of a vaccine against avian bird flu using Lactococcus lactis cells as antigen carriers.
    Źródło:
    Acta Biochimica Polonica; 2014, 61, 3; 609-614
    0001-527X
    Pojawia się w:
    Acta Biochimica Polonica
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Expression, purification and characterization of glycosylated influenza H5N1 hemagglutinin produced in Pichia pastoris
    Autorzy:
    Kopera, Edyta
    Dwornyk, Angela
    Kosson, Piotr
    Florys, Katarzyna
    Sączyńska, Violetta
    Dębski, Janusz
    Cecuda-Adamczewska, Violetta
    Szewczyk, Bogusław
    Zagórski-Ostoja, Włodzimierz
    Grzelak, Krystyna
    Powiązania:
    https://bibliotekanauki.pl/articles/1039268.pdf
    Data publikacji:
    2014
    Wydawca:
    Polskie Towarzystwo Biochemiczne
    Tematy:
    Avian influenza
    H5N1
    recombinant hemagglutinin
    Pichia pastoris
    Opis:
    The A/swan/Poland/305-135V08/2006 (H5N1-subtype) hemagglutinin (HA) gene was cloned and expressed in yeast Pichia pastoris (P. pastoris). The HA cDNA lacking the C-terminal transmembrane anchor-coding sequence was fused to an α-factor leader peptide and placed under control of the methanol-inducible P. pastoris alcohol oxidase 1 (AOX1) promoter. Two P. pastoris strains: SMD 1168 and KM 71 were used for protein expression. Recombinant HA protein was secreted into the culture medium reaching an approximately 15 mg/L (KM 71 strain). Fusion protein with a His6 tag was purified to homogeneity in one step affinity chromatography. SDS-PAGE and MS/MS analysis indicated that the protein is cleaved into HA1 and HA2 domains linked by a disulfide bond. Analysis of the N-linked glycans revealed that the overexpressed HA is fully glycosylated at the same sites as the native HA in the vaccine strain. Immunological activity of the hemagglutinin protein was tested in mice, where rHA elicited a high immune response.
    Źródło:
    Acta Biochimica Polonica; 2014, 61, 3; 597-602
    0001-527X
    Pojawia się w:
    Acta Biochimica Polonica
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Hydroizolacja elementów budowli w wybranych okresach historii architektury czyli o uszczelnieniach z nietypowych materiałów, o dawnych impregnatach, drenażach i pokrewnych rozwiązaniach budowlanych
    Autorzy:
    Szewczyk, Jarosław
    Barek, Radosław
    Szuba, Bogusław
    Powiązania:
    https://bibliotekanauki.pl/books/2098327.pdf
    Data publikacji:
    2019
    Wydawca:
    Politechnika Białostocka. Oficyna Wydawnicza Politechniki Białostockiej
    Opis:
    Na podstawie dawnego piśmiennictwa technicznego, poradnikowego i encyklopedycznego, którego analizę poparto współczesnymi opracowaniami etnograficznymi i archeologicznymi, rozpoznano dawne, dziś już nieużywane, techniki hydroizolacji budynków. Opisano służące temu celowi materiały budowlane. W opisie skoncentrowano się na rozwiązaniach obecnie uchodzących za nietypowe czy alternatywne, opisywanych w publikacjach do połowy XIX wieku. Uwzględniono też i scharakteryzowano odnośne surowce materiałowe, takie jak glina, kora brzozowa, węgiel drzewny, dziegieć i tym podobne. Rozpoznanie i porównanie rozpoznanych surowców, budulców i rozwiązań technologicznych uzasadnia wniosek o znacznej różnorodności dawnych sposobów ochrony budynków przed wilgocią. W świetle takiej oceny walka z wilgocią wydaje się jednym z najsilniejszych stymulatorów postępu technologicznego i wręcz motorem rozwoju cywilizacji.
    An extended bibliography survey has supported comprehensive assessment of old and nonstandard materials and techniques for building waterproofing, impregnation and sealing. Vernacular uses of raw materials such as clay, birch bark, birch or pine tar, charcoal etc., were mentioned by ancient writers and also evidenced by contemporary ethnographers and archaeologists. Such mentions and evidences substantiate the conclusion that techniques of waterproofing, impregnation and sealing altogether constituted extremely multifarious group of building technologies, being essential for construction technology development throughout the whole history of civilization.
    Dostawca treści:
    Biblioteka Nauki
    Książka
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