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    Wyświetlanie 1-5 z 5
    Tytuł:
    Podocyturia jako nowy marker w diagnostyce uszkodzeń ciałek nerkowych w medycynie weterynaryjnej
    Podocyturia as a new marker in renal corpuscles damage diagnostic tests in veterinary medicine
    Autorzy:
    Szczepankiewicz, B.
    Paslawska, U.
    Slawuta, P.
    Madej, J.P.
    Nowak, M.
    Bachor, R.
    Marycz, K.
    Gebarowski, T.
    Czyzewska-Buczynska, A.
    Szewczuk, Z.
    Powiązania:
    https://bibliotekanauki.pl/articles/860245.pdf
    Data publikacji:
    2019
    Wydawca:
    Krajowa Izba Lekarsko-Weterynaryjna
    Źródło:
    Życie Weterynaryjne; 2019, 94, 10
    0137-6810
    Pojawia się w:
    Życie Weterynaryjne
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Spektrometria mas w analizie białek i peptydów : znaczniki jonizacyjne
    Mass spectrometry in analysis of peptides and proteins : ionization markers
    Autorzy:
    Bąchor, R.
    Biernat, M.
    Cebrat, M.
    Kijewska, M.
    Kluczyk, A.
    Kuczer, M.
    Paluch, A.
    Waliczek, M.
    Wierzbicka, M.
    Stefanowicz, P.
    Szewczuk, Z.
    Powiązania:
    https://bibliotekanauki.pl/articles/171509.pdf
    Data publikacji:
    2018
    Wydawca:
    Polskie Towarzystwo Chemiczne
    Tematy:
    spektrometria mas
    modyfikacje potranslacyjne
    znaczniki jonizacyjne
    fosforylacja
    glikacja
    biblioteki kombinatoryczne
    mass spectrometry
    post-translational modifications
    ionization markers
    phosphorylation
    glycation
    combinatorial libraries
    Opis:
    High sensitivity, accuracy, and ability to provide structural information makes mass spectrometry (MS) the method of choice for both qualitative and quantitative analysis in proteome research. Peptide sequencing by tandem mass spectrometry (MS/MS) was successfully applied to discover new peptide sequences and modifications. Insufficient ionization of some peptides is one of the main limitations of MS- based peptide identification. The development of sensitive detection techniques for the efficient analysis of such samples is very important. Differences in ionizability cause difficulties in quantification studies, which could be overcome by derivatization of peptides to improve both the detectability and the selectivity of an analysis. Incorporation of ionization markers and isotopic labels (particularly the isobaric tags) is often used for this reason. Isobaric labeling reagents (including commercially available iTRAQ, TMT, DiLeu and DiART) have found a wide application in quantitative proteomics. Mass spectrometry is a very good tool for the determination of posttranslational modifications (PTMs), but the modified proteins are usually present in low concentrations. The development of ionization tags specific to a particular PTM and suitable for sensitive analysis of the modified proteins is required. For the analysis of phosphorylated peptides, a combination of β-elimination and the reaction of resulting α,β-dehydroamino acid residues with the nucleophilic thiol group could be used to detect a labile PTM. Such reaction may be used to introduce derivatizing reagents at the original site of phosphorylation, to enhance ionization in MS analysis. Glycation and glycosylation of proteins are other very important PTMs associated with many natural processes as well as diseases. We have designed and synthesized bifunctional quaternary ammonium salt derivatives of phenylboronic acids for selective detection of carbohydrates and peptide-derived Amadori products by ESI-MS. The attachment of a fixed charge (e.g. in a form of a quaternary ammonium salt) to the amino groups in peptides leads to the enhancement of a precursor ion signal in mass spectra. We have developed several new QAS-containing ionization reagents including bicyclic tags with DABCO, ABCO or azoniaspiro groups. It is worth noting that 2,4,6-substituted pyrylium salts react with amino groups in peptides introducing a stable positive charge and improve peptide detection by MS. The newly developed ionization tags were successfully applied for the analysis of OBOC combinatorial libraries as well as for studying possible biomarkers of preeclampsia, a pregnancy disorder.
    Źródło:
    Wiadomości Chemiczne; 2018, 72, 7-8; 609-633
    0043-5104
    2300-0295
    Pojawia się w:
    Wiadomości Chemiczne
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Effect of supplementing selenized yeast to ewes from an organic farm on serum Se concentration in lambs
    Autorzy:
    Pilarczyk, B.
    Szewczuk, M.
    Pilarczyk, R.
    Tomza-Marciniak, A.
    Marciniak, A.
    Dobrzański, Z.
    Bąkowska, M.
    Powiązania:
    https://bibliotekanauki.pl/articles/962847.pdf
    Data publikacji:
    2016
    Wydawca:
    Uniwersytet Warmińsko-Mazurski w Olsztynie / Polskie Towarzystwo Magnezologiczne im. Prof. Juliana Aleksandrowicza
    Opis:
    Our objective has been to determine the effect of supplementing selenized yeast to ewes kept on a poor diet, composed of fodder from periodically flooded meadows, on the reproductive results of ewes and the serum Se concentration in lambs, their body weight and daily weight gain. The study involved 25 ewes and 25 lambs, which were divided into two groups: experimental (12 ewes) and control (13 ewes). Their lambs were also assigned to different groups. Prior to supplementation, all the sheep from the two groups were analysed for selenium concentrations in the serum. The serum selenium concentrations were determined fluorometrically using a DAN and Shimadzu RF-5001 PC spectrofluorometer. The excitation wavelength was 376 nm, while the fluorescence emission wavelength was 518 nm. All sheep in the analysed flock were found to be selenium deficient. The supplementation of selenized yeast to the experimental group caused an almost 2.5-fold increase in the serum selenium concentration in the ewes. Lambs from the experimental group were characterized by a significantly higher concentration of selenium at 7 and 14 days of age (P ≤ 0.05) compared to control lambs. Very high (P < 0.001) positive correlations were found between the serum selenium concentration in Se-supplemented ewes and the serum selenium concentration in their lambs at 7 days (rx,y = 0.779) and 14 days of age (rx,y = 0.774). The enrichment of ewes’ diet with selenized yeast contributed to an increase in the serum selenium concentration in their lambs. However, only 14.29% of lambs achieved optimum levels of Se on day 7 after birth. At day 14 after birth, none of the lambs reached the optimum Se concentration. The serum selenium concentration in sheep can be a good indicator of the body’s supply with this element in adult sheep and of a possible selenium status in their offspring. On organic farms, the selenium content in sheep should be monitored constantly.
    Źródło:
    Journal of Elementology; 2016, 21, 4
    1644-2296
    Pojawia się w:
    Journal of Elementology
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Information Exchange Automation in Maritime Transport
    Autorzy:
    Pietrzykowski, Z.
    Banaś, P.
    Wójcik, A.
    Szewczuk, T.
    Powiązania:
    https://bibliotekanauki.pl/articles/116246.pdf
    Data publikacji:
    2014
    Wydawca:
    Uniwersytet Morski w Gdyni. Wydział Nawigacyjny
    Tematy:
    maritime transport
    Information Exchange
    safety at sea
    Information Technology (IT)
    Intelligent Communication
    Automatic Communication
    IHO S-100
    e-Navigation
    Opis:
    To ensure the safety of maritime transport the access to information must be provided through a commonly used services. However, an equally important task is to determine or agree on interpretation of incoming data and assessment of a current and predicted navigational situation and, in further step, intentions of the participants in the transport process. Thanks to the standardization of information format, automatic information exchange gets increasingly wider. Another step ahead is automatic interpretation of information and automation of negotiation processes - intelligent communication. Rapid development of IT and ICT technologies creates such opportunities. This article presents the results of research on a system of automatic communication and co-operation in maritime transport.
    Źródło:
    TransNav : International Journal on Marine Navigation and Safety of Sea Transportation; 2014, 8, 2; 189-193
    2083-6473
    2083-6481
    Pojawia się w:
    TransNav : International Journal on Marine Navigation and Safety of Sea Transportation
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Dynamic model with reaction nodes of tracked agricultural tractor
    Model dynamiczny z ogniwami reakcyjnymi układu napędowego ciągnika gąsienicowego
    Autorzy:
    Godżajew, Z. A.
    Liaszenko, M. W.
    Szechowcow, W. W.
    Szewczuk, W. P.
    Sokołow-Dobriew, N. S.
    Michałowski, B.
    Powiązania:
    https://bibliotekanauki.pl/articles/244389.pdf
    Data publikacji:
    2007
    Wydawca:
    Instytut Techniczny Wojsk Lotniczych
    Tematy:
    ciągnik gąsienicowy
    układ napędowy
    model matematyczny
    obciążenia dynamiczne
    tracked tractor
    transmission system
    mathematical model
    dynamic loads
    Opis:
    In the paper there were described assumptions and methodology of building the mathematical model of the transmission system of tracked agricultural tractor. There was presented model including 34 mass elements jointed by elasto-damping elements. As a result of calculations were estimated parameters of those elements basing on measurements of real object as well as were formulated differential equations of movement taking into account the underslung working equipment. As well were described the method of modelling transmission components, especially taking into consideration reaction moments between elements transmitting power and the chassis of the tractor. There was presented the way of defining the course of engine torque and estimated the main harmonics, with ultimate influence on dynamic loads of transmission. In the range of examination of the transmission operating loads, model takes into account the inputting changes of clutch friction torque during switching it on and off. Model gives ability to analyze the dynamic loads of transmission system depending on clutch slide. Model also allows existing clearances, what gives the possibility of analysis of abrasive wear influence on functioning of the transmission. Model enables the simulation of turning mechanism brakes operation during manoeuvring movement of the tractor.
    Źródło:
    Journal of KONES; 2007, 14, 4; 101-112
    1231-4005
    2354-0133
    Pojawia się w:
    Journal of KONES
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
      Wyświetlanie 1-5 z 5

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