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    Wyświetlanie 1-7 z 7
    Tytuł:
    Activity of phosphofructokinase and phosphoenolopyruvate carboxylase in Norway maple (Acer platanoides L.) seeds during dormancy breaking
    Autorzy:
    Krawiarz, K
    Szczotka, Z.
    Powiązania:
    https://bibliotekanauki.pl/articles/41615.pdf
    Data publikacji:
    2002
    Wydawca:
    Polska Akademia Nauk. Instytut Dendrologii PAN
    Tematy:
    phosphoenolopyruvate carboxylase
    dormancy breaking
    glucose
    activity
    sucrose
    phosphofructokinase
    Norway maple
    Acer platanoides
    seed
    Opis:
    We analysed changes in the activity of phosphofructokinase (PFK) and phosphoenolopyruvate carboxylase (PEPC), and in the glucose and sucrose contents of Norway maple seeds stratified at 3°C (dormancy broken) or treated at temperature 15°C (dormancy not broken). We found that changes in the activity of enzymes are not linear, and 2-3 stages may be distinguished. Dormancy breaking and seed germination is associated with a high activity of PFK and PEPC, and a high glucose level in embryo axes.
    Źródło:
    Dendrobiology; 2002, 47
    1641-1307
    Pojawia się w:
    Dendrobiology
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Application of real-time PCR for detection of Lawsonia intracellularis and Brachyspira hyodysenteriage in fecal samples from pigs
    Autorzy:
    Zmudzki, J.
    Szczotka, A.
    Podgorska, K.
    Nowak, A.
    Grzesiak, A.
    Dors, A.
    Pejsak, Z.
    Powiązania:
    https://bibliotekanauki.pl/articles/30775.pdf
    Data publikacji:
    2012
    Wydawca:
    Polska Akademia Nauk. Czytelnia Czasopism PAN
    Opis:
    The aim of the study was to develop and validate real-time PCR method for the quantification of Lawsonia intracellularis and Brachyspira hyodysenteriae in porcine feces. Before the optimization process was performed two different extraction methods were compared to select the more efficient one. Based on the results achieved at this stage the boiling procedure was rejected and a commercially available silica-membrane based method was chosen for further analysis. The primers and the Taqman probe for B. hyodysenteriae and L. intracellularis were based on the sequence of NADH oxidase gene and 16S rDNA gene, respectively. The detection limit of the real-time PCR for suspension of feces inoculated with B. hyodysenteriae and L. intracellularis was determined to be 1.5x103 CFU/ml and 6.5x101 CFU/ml, respectively. The results of this study demonstrate that our real-time PCR is able to detect low number of B. hyodysenteriae and L. intracellularis cells which is satisfying in routine diagnosis of swine dysentery and proliferative enteropathy. Therefore, it is possible to identify both subclinically infected pigs and those representing an acute form of mentioned diseases. In summary, the quantitative real-time PCR is useful for routine diagnosis of L. intracellularis and B. hyodysenteriae. Compared to conventional PCR, the new validated quantification method based on real-time PCR is fast and with reduced risk of laboratory contamination. The novel technique is specific and even more sensitive than the previously used one. Furthermore, the new real-time PCR enables quick detection and quantification of both pathogens in fecal samples, which helps to estimate the health status of a pig herd.
    Źródło:
    Polish Journal of Veterinary Sciences; 2012, 15, 2
    1505-1773
    Pojawia się w:
    Polish Journal of Veterinary Sciences
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Blood dendritic cells in cattle infected with bovine leukemia virus (BLV): isolation and phenotyping
    Autorzy:
    Szczotka, M.
    Kuzmak, J.
    Kostro, K.
    Bednarek, D.
    Purzycka, M.
    Powiązania:
    https://bibliotekanauki.pl/articles/30807.pdf
    Data publikacji:
    2012
    Wydawca:
    Polska Akademia Nauk. Czytelnia Czasopism PAN
    Opis:
    Dendritic cells (DCs) are most potent antigen presenting cells (APCs) with unique ability to prime effective immune responses. They express higher levels of MHC class II and accesory molecules on their surface, than other professional APCs. The investigations were performed on DCs generated from blood with the use of microbeads magnetically labeled with mouse anti human CD14. Flow cytometry was applied for determination of DCs immunophenotype in healthy and naturally infected with BLV cattle. For immunophenotyping mouse monoclonal antibodies anti bovine: CD11a, CD11b, CD11c, MHC-I and MHC-II were used. Our results demonstrated that dendritic cells infected with BLV expressed very high percentage of determinants: CD11a, CD11b, CD11c, MHC-I and MHC-II class. Leukaemic DCs exhibited DCs morphology and had a phenotype of mature DCs. The expression of gp51 glycoprotein of BLV on leukaemic DCs was detected in flow cytometry investigations.
    Źródło:
    Polish Journal of Veterinary Sciences; 2012, 15, 4
    1505-1773
    Pojawia się w:
    Polish Journal of Veterinary Sciences
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
      Wyświetlanie 1-7 z 7

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